primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ycf2	1	GGAACCATATCACATCCCGG	ACTGTCTACGGTTCGATTGC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.192834	1710	2709	1793-1793	0.500000	58.5
ycf2_p2	ycf2	2	GGAACCATATCACATCCCGG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	57.8	58.0	0.0	7.6	0.194205	1710	2709	1226-1226	0.500000	58.4
ycf2_p3	ycf2	3	CGGAACCATATCACATCCCG	ACTGTCTACGGTTCGATTGC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.212063	1710	2709	1794-1794	0.500000	58.3
ycf2_p4	ycf2	4	CGGAACCATATCACATCCCG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.2	58.0	0.0	7.6	0.213433	1710	2709	1227-1227	0.500000	58.3
ycf2_p5	ycf2	5	GGAACCATATCACATCCCGG	TTCTACCCTTTCCCGGAAGT	20	20	55.0	50.0	57.8	58.0	0.0	0.7	0.219440	1710	2709	1614-1614	0.500000	58.2
rpl16_p1	rpl16	1	TGTTCAATCAAAACAAAACCA	AAACCGGCACCTGTTATTGT	21	20	28.6	45.0	52.9	58.0	0.0	0.0	6.103635	6524	7880	1492-1492	0.500000	20.0
rpl16_p2	rpl16	2	TGTTCAATCAAAACAAAACCA	AACCGGCACCTGTTATTGTT	21	20	28.6	45.0	52.9	58.0	0.0	0.0	6.103635	6524	7880	1491-1491	0.500000	20.0
rpl16_p3	rpl16	3	TGTTCAATCAAAACAAAACCA	ACCGGCACCTGTTATTGTTT	21	20	28.6	45.0	52.9	58.0	0.0	0.0	6.103635	6524	7880	1490-1490	0.500000	20.0
rpl16_p4	rpl16	4	TGTTCAATCAAAACAAAACCA	TCCAAAGAAAGAAACCGGCA	21	20	28.6	45.0	52.9	57.9	0.0	0.0	6.153392	6524	7880	1503-1503	0.500000	20.0
rpl16_p5	rpl16	5	TGTTCAATCAAAACAAAACCA	AAAGAAAGAAACCGGCACCT	21	20	28.6	45.0	52.9	57.9	0.0	0.0	6.160707	6524	7880	1500-1500	0.500000	20.0
rpoA_p1	rpoA	1	TGTAGACCTCCGAAAAAGCG	ACGACCTGTGTATCTAGGGA	20	20	50.0	50.0	57.9	56.9	0.0	0.0	1.191547	10178	11239	1252-1252	0.500000	50.5
rpoA_p2	rpoA	2	TGTAGACCTCCGAAAAAGCG	CACGACCTGTGTATCTAGGG	20	20	50.0	55.0	57.9	56.8	0.0	0.0	1.233029	10178	11239	1253-1253	0.500000	50.1
rpoA_p3	rpoA	3	CGAAAAAGCGACGTGTGTAG	ACGACCTGTGTATCTAGGGA	20	20	50.0	50.0	57.8	56.9	0.0	0.0	1.309463	10178	11239	1242-1242	0.500000	49.5
rpoA_p4	rpoA	4	CGAAAAAGCGACGTGTGTAG	CACGACCTGTGTATCTAGGG	20	20	50.0	55.0	57.8	56.8	0.0	0.0	1.350945	10178	11239	1243-1243	0.500000	49.2
rpoA_p5	rpoA	5	TGTAGACCTCCGAAAAAGCG	CACGACCTGTGTATCTAGGGA	20	21	50.0	52.4	57.9	58.3	0.0	0.0	1.423092	10178	11239	1253-1253	0.500000	48.6
psbH_p1	psbH	1	CGCGGTCGAACTACCAAAAT	ACAAAGTCAACAGCTCTCAATG	20	22	50.0	40.9	58.9	57.2	0.0	0.0	3.752369	14321	14542	360-360	0.500000	30.0
psbH_p2	psbH	2	CGCGGTCGAACTACCAAAAT	ACAAAGTCAACAGCTCTCAA	20	20	50.0	40.0	58.9	55.1	0.0	0.0	3.793889	14321	14542	360-360	0.500000	29.6
psbH_p3	psbH	3	CGCGGTCGAACTACCAAAAT	CAAAGTCAACAGCTCTCAATGA	20	22	50.0	40.9	58.9	56.9	0.0	0.0	4.013178	14321	14542	359-359	0.500000	27.9
psbH_p4	psbH	4	CGCGGTCGAACTACCAAAAT	AGGGTTGCTGTTTCCATTAT	20	20	50.0	40.0	58.9	54.9	0.0	0.0	4.049365	14321	14542	442-442	0.500000	27.6
psbH_p5	psbH	5	CGCGGTCGAACTACCAAAAT	ACAAAGTCAACAGCTCTCAAT	20	21	50.0	38.1	58.9	55.7	0.0	0.0	4.205428	14321	14542	360-360	0.500000	26.4
psbN_p1	psbN	1	CATTGAGAGCTGTTGACTTTGT	GAGAACCGCCTAAAGTTCCA	22	20	40.9	50.0	57.2	57.5	0.0	0.0	3.293112	14645	14776	337-337	0.500000	33.7
psbN_p2	psbN	2	TTGAGAGCTGTTGACTTTGT	GAGAACCGCCTAAAGTTCCA	20	20	40.0	50.0	55.1	57.5	0.0	0.0	3.334632	14645	14776	335-335	0.500000	33.3
psbN_p3	psbN	3	CATTGAGAGCTGTTGACTTTGT	AACCGCCTAAAGTTCCAACT	22	20	40.9	45.0	57.2	57.3	0.0	0.0	3.476927	14645	14776	334-334	0.500000	32.2
psbN_p4	psbN	4	TTGAGAGCTGTTGACTTTGT	AACCGCCTAAAGTTCCAACT	20	20	40.0	45.0	55.1	57.3	0.0	0.0	3.518448	14645	14776	332-332	0.500000	31.9
psbN_p5	psbN	5	TCATTGAGAGCTGTTGACTTTG	GAGAACCGCCTAAAGTTCCA	22	20	40.9	50.0	56.9	57.5	0.0	0.0	3.553921	14645	14776	338-338	0.500000	31.6
psbB-trnH-GUG_p1	psbB-trnH-GUG	1	CGATACCAAGGCAAACCCAT	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.2	58.1	0.0	0.2	0.366212	16692	19578	2980-2980	0.500000	57.1
psbB-trnH-GUG_p2	psbB-trnH-GUG	2	CGATACCAAGGCAAACCCAT	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.370574	16692	19578	2966-2966	0.500000	57.0
psbB-trnH-GUG_p3	psbB-trnH-GUG	3	CGATACCAAGGCAAACCCAT	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.372081	16692	19578	2965-2965	0.500000	57.0
psbB-trnH-GUG_p4	psbB-trnH-GUG	4	CGATACCAAGGCAAACCCAT	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.372081	16692	19578	2967-2967	0.500000	57.0
psbB-trnH-GUG_p5	psbB-trnH-GUG	5	ACACGATACCAAGGCAAACC	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.5	58.1	0.0	0.2	0.598596	16692	19578	2983-2983	0.500000	55.2
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	19654	19842	306-336	1.000000	80.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	19654	19842	256-286	1.000000	80.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	19654	19842	320-350	1.000000	80.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	19654	19842	319-349	1.000000	80.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	19654	19842	321-351	1.000000	80.3
trnK_p1	trnK	1	TCTAGACCTATCCAAGCTTT	ACATAAGAACGAAGAGGTGGTC	20	22	40.0	45.5	52.7	57.8	0.0	0.0	7.473882	21104	23705	2758-2758	1.000000	40.6
trnK_p2	trnK	2	TCTAGACCTATCCAAGCTTTT	ACATAAGAACGAAGAGGTGGTC	21	22	38.1	45.5	53.7	57.8	0.0	0.0	7.526852	21104	23705	2758-2758	1.000000	40.6
trnK_p3	trnK	3	TCTAGACCTATCCAAGCTTT	ACATAAGAACGAAGAGGTGGT	20	21	40.0	42.9	52.7	56.7	0.0	0.0	7.614321	21104	23705	2758-2758	1.000000	40.6
trnK_p4	trnK	4	TCTAGACCTATCCAAGCTTTTT	ACATAAGAACGAAGAGGTGGTC	22	22	36.4	45.5	54.5	57.8	0.0	0.0	7.666992	21104	23705	2758-2758	1.000000	40.6
trnK_p5	trnK	5	TCTAGACCTATCCAAGCTTTT	ACATAAGAACGAAGAGGTGGT	21	21	38.1	42.9	53.7	56.7	0.0	0.0	7.667292	21104	23705	2758-2758	1.000000	40.6
matK-trnK_p1	matK-trnK	1	AACCATGATCATGCGCAAGT	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	58.5	58.2	27.2	17.1	0.701563	22923	23668	879-880	1.000000	74.8
matK-trnK_p2	matK-trnK	2	AACCATGATCATGCGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	58.5	58.2	27.2	17.1	0.701563	22923	23668	878-879	1.000000	74.8
matK-trnK_p3	matK-trnK	3	AAACCATGATCATGCGCAAG	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.4	58.2	27.2	17.1	0.740610	22923	23668	880-881	1.000000	74.5
matK-trnK_p4	matK-trnK	4	AAACCATGATCATGCGCAAG	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.4	58.2	27.2	17.1	0.740610	22923	23668	879-880	1.000000	74.5
matK-trnK_p5	matK-trnK	5	AACCATGATCATGCGCAAGT	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	58.5	57.5	27.2	0.0	0.993901	22923	23668	892-893	1.000000	72.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	47800	48518	760-775	1.000000	79.7
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	47800	48518	821-836	1.000000	78.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	47800	48518	778-793	1.000000	77.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	47800	48518	839-854	1.000000	75.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	47800	48518	759-774	1.000000	73.8
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.250489	48609	49766	1332-1346	1.000000	79.1
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.450004	48609	49766	1271-1285	1.000000	77.5
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.544620	48609	49766	1331-1345	1.000000	76.7
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.744134	48609	49766	1270-1284	1.000000	75.2
petN-psbM_p5	petN-psbM	5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	57.1	57.9	0.0	0.0	0.991926	48609	49766	1333-1347	1.000000	73.1
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	57.9	58.1	4.1	11.5	0.219123	61826	62521	816-826	1.000000	78.6
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	61826	62521	803-813	1.000000	78.2
psaA-ycf3_p3	psaA-ycf3	3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	58.2	58.1	0.0	11.5	0.282564	61826	62521	839-849	1.000000	78.1
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	61826	62521	807-817	1.000000	77.9
psaA-ycf3_p5	psaA-ycf3	5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.342602	61826	62521	826-836	1.000000	77.6
cemA_p1	cemA	1	TTGGATGGCGTAGAGTCAAC	ACATGCTTTGCTTCTGTACAAC	20	22	50.0	40.9	57.6	57.8	0.0	0.0	2.608996	80788	81477	828-828	1.000000	59.4
cemA_p2	cemA	2	TGGATGGCGTAGAGTCAACT	ACATGCTTTGCTTCTGTACAAC	20	22	50.0	40.9	58.4	57.8	0.0	0.0	2.671813	80788	81477	827-827	1.000000	58.9
cemA_p3	cemA	3	TTGGATGGCGTAGAGTCAAC	ACATGCTTTGCTTCTGTACA	20	20	50.0	40.0	57.6	55.6	0.0	0.0	2.802676	80788	81477	828-828	1.000000	57.9
cemA_p4	cemA	4	TGGATGGCGTAGAGTCAACT	ACATGCTTTGCTTCTGTACA	20	20	50.0	40.0	58.4	55.6	0.0	0.0	2.865494	80788	81477	827-827	1.000000	57.4
cemA_p5	cemA	5	TTGGATGGCGTAGAGTCAAC	TGCTTTGCTTCTGTACAACT	20	20	50.0	40.0	57.6	55.5	0.0	0.0	2.873568	80788	81477	825-825	1.000000	57.3
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	82686	83706	1148-1211	1.000000	74.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	82686	83706	1135-1198	1.000000	74.4
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	82686	83706	1149-1212	1.000000	74.1
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	82686	83706	1136-1199	1.000000	73.7
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	82686	83706	1153-1216	1.000000	73.2
clpP_p1	clpP	1	GGGCTGGTTTAGATCGATCC	TACGTTTCATCACCACCACC	20	20	55.0	50.0	57.8	57.8	21.7	0.0	0.344192	89583	91671	2288-2288	0.500000	57.2
clpP_p2	clpP	2	GGGCTGGTTTAGATCGATCC	GGATCGACGGGAAGATTTGT	20	20	55.0	50.0	57.8	57.7	21.7	0.0	0.482465	89583	91671	2316-2316	0.500000	56.1
clpP_p3	clpP	3	GGGCTGGTTTAGATCGATCC	CGTTTCATCACCACCACCTT	20	20	55.0	50.0	57.8	58.4	21.7	0.0	0.566737	89583	91671	2286-2286	0.500000	55.5
clpP_p4	clpP	4	GAAATCGAGAATTTGCGCGA	TACGTTTCATCACCACCACC	20	20	45.0	50.0	57.6	57.8	12.1	0.0	0.608536	89583	91671	2203-2203	0.500000	55.1
clpP_p5	clpP	5	GGAAATCGAGAATTTGCGCG	TACGTTTCATCACCACCACC	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.716119	89583	91671	2204-2204	0.500000	54.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	117290	118227	1057-1062	1.000000	80.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	117290	118227	1058-1063	1.000000	80.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	117290	118227	1078-1083	1.000000	79.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	117290	118227	1080-1085	1.000000	79.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	117290	118227	1067-1072	1.000000	79.5