primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	76	654	679-727	1.000000	89.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ACTGCCTTAATCCACTTGGC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.193257	76	654	680-728	1.000000	89.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CTGCCTTAATCCACTTGGCT	TATCATCAACCGCGCTAACC	20	20	50.0	50.0	57.9	58.1	0.0	5.6	0.274113	76	654	690-738	1.000000	88.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACTGCCTTAATCCACTTGGC	TATCATCAACCGCGCTAACC	20	20	50.0	50.0	58.2	58.1	0.0	5.6	0.299900	76	654	691-739	1.000000	88.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CTGCCTTAATCCACTTGGCT	CTGATATCATCAACCGCGCT	20	20	50.0	50.0	57.9	58.2	0.0	5.7	0.346531	76	654	694-742	1.000000	87.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTACCAACGGTATGGACGA	TTCTTGAAAAGGGCGCTCAA	20	20	50.0	45.0	57.9	58.3	10.6	11.4	0.440916	4503	5048	673-982	1.000000	82.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCTACCAACGGTATGGACGA	TTTCTTGAAAAGGGCGCTCA	20	20	50.0	45.0	57.9	58.3	10.6	11.4	0.440916	4503	5048	674-983	1.000000	82.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCTTGAAAAGGGCGCTCAA	20	20	50.0	45.0	58.2	58.3	20.3	11.4	0.482065	4503	5048	608-917	1.000000	82.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCTTGAAAAGGGCGCTCAA	20	20	50.0	45.0	58.2	58.3	20.3	11.4	0.482065	4503	5048	607-916	1.000000	82.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TTTCTTGAAAAGGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	11.4	0.482065	4503	5048	608-917	1.000000	82.0
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	20	20	45.0	45.0	58.0	56.4	0.0	0.0	1.674622	8124	8801	788-800	1.000000	69.5
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AAGGAACGGAAAGAGAGGGA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.662051	8124	8801	842-854	1.000000	69.4
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.2	56.4	0.0	0.0	1.787764	8124	8801	837-849	1.000000	68.5
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AGGAACGGAAAGAGAGGGAT	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	57.7	56.4	0.0	0.0	1.892186	8124	8801	841-853	1.000000	67.6
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AAGGAACGGAAAGAGAGGGA	AAACCGAAAGACCCCTTAACT	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.896174	8124	8801	843-855	1.000000	67.6
atpF-atpH_p1	atpF-atpH	1	TTAAATCCGAAACTCCCGGC	CGGAGGCAGAGGGTAAAATC	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.303545	12788	13283	673-695	1.000000	80.0
atpF-atpH_p2	atpF-atpH	2	TTAAATCCGAAACTCCCGGC	GAGGCAGAGGGTAAAATCCG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.303545	12788	13283	671-693	1.000000	80.0
atpF-atpH_p3	atpF-atpH	3	TTAAATCCGAAACTCCCGGC	GCAGAGGGTAAAATCCGAGG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.303545	12788	13283	668-690	1.000000	80.0
atpF-atpH_p4	atpF-atpH	4	TTAAATCCGAAACTCCCGGC	GGCAGAGGGTAAAATCCGAG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.303545	12788	13283	669-691	1.000000	80.0
atpF-atpH_p5	atpF-atpH	5	CCCAGGGAGACGAAAGAATC	GCAGAGGGTAAAATCCGAGG	20	20	55.0	55.0	57.7	58.0	0.0	0.0	0.362603	12788	13283	635-657	1.000000	79.7
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	TCCTATTCGTGGGCCATTTG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.9	58.1	10.7	0.0	0.110009	36992	37243	445-445	1.000000	82.0
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	TCCTATTCGTGGGCCATTTG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	57.9	58.1	10.7	0.0	0.180254	36992	37243	456-456	1.000000	81.4
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TCCTATTCGTGGGCCATTTG	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.9	58.2	10.7	17.2	0.268263	36992	37243	391-391	1.000000	81.1
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTATTCGTGGGCCATTTGT	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.2	58.1	10.7	0.0	0.295967	36992	37243	444-444	1.000000	80.5
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	CCTATTCGTGGGCCATTTGT	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.2	58.1	10.7	0.0	0.366212	36992	37243	455-455	1.000000	79.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	48386	49225	882-913	1.000000	82.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	48386	49225	870-901	1.000000	82.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	20	20	55.0	50.0	57.9	57.7	0.0	0.0	0.412322	48386	49225	879-910	1.000000	82.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	20	20	50.0	50.0	57.8	57.8	15.5	0.0	0.465562	48386	49225	914-945	1.000000	82.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	48386	49225	902-933	1.000000	81.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	22	20	36.4	50.0	56.1	57.8	0.0	0.0	4.083948	52635	54279	1591-1701	1.000000	54.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCGTGAAGCAGAAACA	AGTGCATGTTGGGTCTTTGA	22	20	36.4	45.0	56.1	57.6	0.0	0.0	4.249971	52635	54279	1658-1768	1.000000	52.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	22	20	36.4	55.0	56.1	57.5	0.0	0.0	4.427720	52635	54279	1590-1700	1.000000	51.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	22	20	36.4	55.0	56.1	58.7	0.0	0.0	4.570014	52635	54279	1579-1689	1.000000	50.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	21	20	38.1	50.0	54.6	57.8	0.0	0.0	4.585537	52635	54279	1590-1700	1.000000	50.0
rrn16_p1	rrn16	1	GGCTCCAAGTTGTTCAGGAA	TGAATCGCCCCATCTATCCT	20	20	50.0	50.0	58.0	58.0	1.4	0.0	0.048771	102947	104437	2390-2393	1.000000	86.2
rrn16_p2	rrn16	2	GGCTCCAAGTTGTTCAGGAA	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	58.0	58.0	1.4	0.0	0.034771	102947	104437	1962-1962	0.750000	69.8
rrn16_p3	rrn16	3	GAGCAAACTCCAGGCGAATA	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040203	102947	104437	1682-1682	0.750000	69.7
rrn16_p4	rrn16	4	GCAAGAAGAAGGCCGTATCA	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040203	102947	104437	2246-2246	0.750000	69.7
rrn16_p5	rrn16	5	GAGCAAACTCCAGGCGAATA	TGAATCGCCCCATCTATCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054203	102947	104437	2113-2113	0.750000	69.6
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	110470	111576	1189-1248	1.000000	82.5
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	110470	111576	1226-1285	1.000000	81.8
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110470	111576	1140-1199	1.000000	81.4
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	TGAGGGTACTCTTGGGAACA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.411395	110470	111576	1241-1300	1.000000	80.7
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.7	58.2	18.7	18.9	0.442458	110470	111576	1177-1236	1.000000	80.7
ndhF-rpl32_p1	ndhF-rpl32	1	GCGGAAGGAAGGGTATGATC	GTACGTTTCTTTGGAACCGC	20	20	55.0	50.0	57.8	57.7	0.0	0.0	0.476593	115249	116117	928-941	1.000000	79.8
ndhF-rpl32_p2	ndhF-rpl32	2	AGCGGAAGGAAGGGTATGAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.497438	115249	116117	929-942	1.000000	79.6
ndhF-rpl32_p3	ndhF-rpl32	3	GCGGAAGGAAGGGTATGATC	GAAAGCGCTTTTACTGCCAC	20	20	55.0	50.0	57.8	58.3	0.0	34.0	0.484876	115249	116117	1003-1016	1.000000	79.4
ndhF-rpl32_p4	ndhF-rpl32	4	AGTCCCACTCCGATTGCTAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.510538	115249	116117	959-972	1.000000	79.4
ndhF-rpl32_p5	ndhF-rpl32	5	AGCGGAAGGAAGGGTATGAT	GAAAGCGCTTTTACTGCCAC	20	20	50.0	50.0	58.2	58.3	0.0	34.0	0.505722	115249	116117	1004-1017	1.000000	79.3
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	132594	133700	1189-1248	1.000000	82.5
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	132594	133700	1226-1285	1.000000	81.8
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	132594	133700	1140-1199	1.000000	81.4
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CCGTTGTTCCTCAGTAGCTC	TGAGGGTACTCTTGGGAACA	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.411395	132594	133700	1241-1300	1.000000	80.7
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	20	20	50.0	55.0	58.2	57.7	18.9	18.7	0.442458	132594	133700	1177-1236	1.000000	80.7
rrn16_p1	rrn16	1	TGAATCGCCCCATCTATCCT	GAGCAAACTCCAGGCGAATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054203	139733	141223	2110-2113	1.000000	86.2
rrn16_p2	rrn16	2	CAGACGTAGCTCGCTTCTTT	GGCTCCAAGTTGTTCAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	1.4	0.034771	139733	141223	1962-1962	0.750000	69.8
rrn16_p3	rrn16	3	CAGACGTAGCTCGCTTCTTT	GAGCAAACTCCAGGCGAATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040203	139733	141223	1682-1682	0.750000	69.7
rrn16_p4	rrn16	4	CAGACGTAGCTCGCTTCTTT	GCAAGAAGAAGGCCGTATCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040203	139733	141223	2246-2246	0.750000	69.7
rrn16_p5	rrn16	5	TGAATCGCCCCATCTATCCT	GGCTCCAAGTTGTTCAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	1.4	0.048771	139733	141223	2393-2393	0.750000	69.6
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CCTCAGCCTACGGGGTATTA	TACTCCGACAGCATCTAGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.022710	141523	143040	2156-2205	0.750000	71.5
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	CCTCAGCCTACGGGGTATTA	CGAATACGGCTTTCCACAGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009953	141523	143040	2657-2706	0.750000	71.3
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	CCTCAGCCTACGGGGTATTA	TCGAATACGGCTTTCCACAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.009953	141523	143040	2658-2707	0.750000	71.3
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	CATTGCGGAAAATTCCCCAC	CGAATACGGCTTTCCACAGA	20	20	50.0	50.0	58.0	58.0	6.6	0.0	0.013334	141523	143040	2831-2880	0.750000	71.2
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CATTGCGGAAAATTCCCCAC	TCGAATACGGCTTTCCACAG	20	20	50.0	50.0	58.0	58.0	6.6	0.0	0.013334	141523	143040	2832-2881	0.750000	71.2
ndhB_p1	ndhB	1	CTGTGGAAAGCCGTATTCGA	TGTAACGACGTAGGGATCCA	20	20	50.0	50.0	58.0	57.9	0.0	15.2	0.131853	144201	146436	2965-2973	1.000000	83.1
ndhB_p2	ndhB	2	TCTGTGGAAAGCCGTATTCG	TGTAACGACGTAGGGATCCA	20	20	50.0	50.0	58.0	57.9	0.0	15.2	0.131853	144201	146436	2966-2974	1.000000	83.1
ndhB_p3	ndhB	3	CTGTGGAAAGCCGTATTCGA	GTTCATAACCCCAGCCCATT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.163762	144201	146436	2802-2817	1.000000	82.8
ndhB_p4	ndhB	4	CTGTGGAAAGCCGTATTCGA	TTACATACGCTCGAATGCCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.143907	144201	146436	2933-2933	0.750000	68.9
ndhB_p5	ndhB	5	TCTGTGGAAAGCCGTATTCG	TTACATACGCTCGAATGCCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.143907	144201	146436	2934-2934	0.750000	68.9