primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-rbcL_p1	psbA-rbcL	1	CGTCTCTCTAAAATTGCAGTCA	ATTTGAGTTTGCCGCAATGG	22	20	40.9	45.0	56.5	57.9	0.0	6.6	3.601541	1477	2299	877-908	1.000000	60.4
psbA-rbcL_p2	psbA-rbcL	2	CGTCTCTCTAAAATTGCAGTCA	TGAGTTTGCCGCAATGGATA	22	20	40.9	45.0	56.5	57.9	0.0	6.6	3.639665	1477	2299	874-905	1.000000	60.1
psbA-rbcL_p3	psbA-rbcL	3	CGTCTCTCTAAAATTGCAGTCA	AGTTTGCCGCAATGGATACT	22	20	40.9	45.0	56.5	57.9	0.0	6.6	3.647145	1477	2299	872-903	1.000000	60.0
psbA-rbcL_p4	psbA-rbcL	4	CGTCTCTCTAAAATTGCAGTCA	GAGTTTGCCGCAATGGATAC	22	20	40.9	50.0	56.5	57.5	0.0	6.6	3.993574	1477	2299	873-904	1.000000	57.2
psbA-rbcL_p5	psbA-rbcL	5	CGTCTCTCTAAAATTGCAGTCAT	ATTTGAGTTTGCCGCAATGG	23	20	39.1	45.0	57.0	57.9	0.0	6.6	4.125268	1477	2299	877-908	1.000000	56.2
trnK (UUU)-rps16_p1	trnK (UUU)-rps16	1	ATCCATTGGACCCAACCCTA	ACGGTAACTATTCGTGTGCC	20	20	50.0	50.0	58.0	58.0	7.8	0.0	0.006031	10373	10883	1336-1336	0.500000	60.0
trnK (UUU)-rps16_p2	trnK (UUU)-rps16	2	TATCCATTGGACCCAACCCT	ACGGTAACTATTCGTGTGCC	20	20	50.0	50.0	58.0	58.0	7.8	0.0	0.006031	10373	10883	1337-1337	0.500000	60.0
trnK (UUU)-rps16_p3	trnK (UUU)-rps16	3	CCTTACCCGTATTCAGGCAC	ACGGTAACTATTCGTGTGCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.050964	10373	10883	1478-1478	0.500000	59.6
trnK (UUU)-rps16_p4	trnK (UUU)-rps16	4	ATCCATTGGACCCAACCCTA	CTCTCACCGAAATCAGGAGC	20	20	50.0	55.0	58.0	58.1	7.8	0.0	0.072355	10373	10883	1105-1105	0.500000	59.4
trnK (UUU)-rps16_p5	trnK (UUU)-rps16	5	TATCCATTGGACCCAACCCT	CTCTCACCGAAATCAGGAGC	20	20	50.0	55.0	58.0	58.1	7.8	0.0	0.072355	10373	10883	1106-1106	0.500000	59.4
ORF213_p1	ORF213	1	TTCGGGAGCGAACATCAATT	ACCGGCCCAATCAAAAATCT	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.170759	11188	11829	980-1409	1.000000	90.6
ORF213_p2	ORF213	2	ATTCTTGCAAGGGATAGCCG	TGGGCGCTAATTGCAATTTG	20	20	50.0	45.0	58.0	58.0	25.7	28.4	0.034488	11188	11829	1713-1713	0.500000	59.7
ORF213_p3	ORF213	3	ATTCTTGCAAGGGATAGCCG	ACCGGCCCAATCAAAAATCT	20	20	50.0	45.0	58.0	58.1	25.7	0.0	0.089937	11188	11829	1625-1625	0.500000	59.3
ORF213_p4	ORF213	4	TTCGGGAGCGAACATCAATT	TGGGCGCTAATTGCAATTTG	20	20	45.0	45.0	57.9	58.0	0.0	28.4	0.115310	11188	11829	1497-1497	0.500000	59.1
ORF213_p5	ORF213	5	TTTTCGAAGCTCGCTTCCTT	TGGGCGCTAATTGCAATTTG	20	20	45.0	45.0	58.1	58.0	22.0	28.4	0.128407	11188	11829	1520-1520	0.500000	59.0
trnS (GCU)-trnG (UCC)_p1	trnS (GCU)-trnG (UCC)	1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	13503	14155	776-808	1.000000	58.6
trnS (GCU)-trnG (UCC)_p2	trnS (GCU)-trnG (UCC)	2	ACGAAAACGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.701161	13503	14155	782-814	1.000000	58.4
trnS (GCU)-trnG (UCC)_p3	trnS (GCU)-trnG (UCC)	3	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.2	57.3	0.0	0.0	3.819906	13503	14155	779-811	1.000000	57.5
trnS (GCU)-trnG (UCC)_p4	trnS (GCU)-trnG (UCC)	4	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.2	57.3	0.0	0.0	3.819906	13503	14155	778-810	1.000000	57.5
trnS (GCU)-trnG (UCC)_p5	trnS (GCU)-trnG (UCC)	5	ACGAAAACGGAAAGAGAGGG	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	57.8	57.3	0.0	0.0	3.833877	13503	14155	784-816	1.000000	57.3
ORF342_p1	ORF342	1	ACAGCCGTACCATTAACGTC	ACTTCGTCCCCAGACTACAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.005799	31254	32282	1780-1780	0.500000	60.0
ORF342_p2	ORF342	2	ACAGCCGTACCATTAACGTC	TTCGTCCCCAGACTACAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.005799	31254	32282	1778-1778	0.500000	60.0
ORF342_p3	ORF342	3	CACGAACAGCCGTACCATTA	ACTTCGTCCCCAGACTACAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006322	31254	32282	1785-1785	0.500000	59.9
ORF342_p4	ORF342	4	CACGAACAGCCGTACCATTA	TTCGTCCCCAGACTACAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006322	31254	32282	1783-1783	0.500000	59.9
ORF342_p5	ORF342	5	ACAGCCGTACCATTAACGTC	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019173	31254	32282	1630-1630	0.500000	59.8
psbM-trnD (GUC)_p1	psbM-trnD (GUC)	1	ACTGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	22	20	40.9	55.0	57.4	57.8	0.0	6.3	2.773008	33590	34510	1019-1035	1.000000	68.0
psbM-trnD (GUC)_p2	psbM-trnD (GUC)	2	ACTGCCAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.4	58.5	0.0	5.4	3.052006	33590	34510	992-1008	1.000000	66.0
psbM-trnD (GUC)_p3	psbM-trnD (GUC)	3	ACTGCCAGAATATTGACTTCCA	TCCCTTAGTTCCCTGGGATT	22	20	40.9	50.0	57.4	57.3	0.0	17.9	3.255113	33590	34510	1016-1032	1.000000	64.2
psbM-trnD (GUC)_p4	psbM-trnD (GUC)	4	AACTGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	23	20	39.1	55.0	58.1	57.8	0.0	6.3	3.276128	33590	34510	1020-1036	1.000000	64.0
psbM-trnD (GUC)_p5	psbM-trnD (GUC)	5	ACTGCCAGAATATTGACTTCCA	CCCTTAGTTCCCTGGGATTG	22	20	40.9	55.0	57.4	57.3	0.0	17.9	3.311344	33590	34510	1015-1031	1.000000	63.8
trnT (GGU)-psbD_p1	trnT (GGU)-psbD	1	AGTTCAAAACCGAAAAGGGC	TTTACCAAGGGCTATAGTCA	20	20	45.0	40.0	57.1	53.0	0.0	6.7	5.873190	35160	36240	1123-1155	1.000000	49.8
trnT (GGU)-psbD_p2	trnT (GGU)-psbD	2	GTTCAAAACCGAAAAGGGCT	TTTACCAAGGGCTATAGTCA	20	20	45.0	40.0	57.1	53.0	0.0	6.7	5.873190	35160	36240	1122-1154	1.000000	49.8
trnT (GGU)-psbD_p3	trnT (GGU)-psbD	3	AGTTCAAAACCGAAAAGGGC	TTACCAAGGGCTATAGTCAT	20	20	45.0	40.0	57.1	52.8	0.0	6.7	6.124550	35160	36240	1122-1154	1.000000	49.8
trnT (GGU)-psbD_p4	trnT (GGU)-psbD	4	GTTCAAAACCGAAAAGGGCT	TTACCAAGGGCTATAGTCAT	20	20	45.0	40.0	57.1	52.8	0.0	6.7	6.124550	35160	36240	1121-1153	1.000000	49.8
trnT (GGU)-psbD_p5	trnT (GGU)-psbD	5	GTTCAAAACCGAAAAGGGCT	TTTACCAAGGGCTATAGTCAT	20	21	45.0	38.1	57.1	53.7	0.0	6.7	6.175773	35160	36240	1122-1154	1.000000	49.8
pafI-trnS (GGA)_p1	pafI-trnS (GGA)	1	GCCTAACCATTCTTCGTCGT	AGGTACGTTGGGTGAAGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019980	47585	48480	1473-1473	0.500000	59.8
pafI-trnS (GGA)_p2	pafI-trnS (GGA)	2	GCCTAACCATTCTTCGTCGT	TAGGTACGTTGGGTGAAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019980	47585	48480	1474-1474	0.500000	59.8
pafI-trnS (GGA)_p3	pafI-trnS (GGA)	3	TTTTTCCCCTGGAGTTGTCG	AGGTACGTTGGGTGAAGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038350	47585	48480	1102-1102	0.500000	59.7
pafI-trnS (GGA)_p4	pafI-trnS (GGA)	4	TTTTTCCCCTGGAGTTGTCG	TAGGTACGTTGGGTGAAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038350	47585	48480	1103-1103	0.500000	59.7
pafI-trnS (GGA)_p5	pafI-trnS (GGA)	5	GCCTAACCATTCTTCGTCGT	GTTATCGCTGCAAACCCCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038651	47585	48480	1855-1855	0.500000	59.7
trnT (UGU)-trnL (UAA)_p1	trnT (UGU)-trnL (UAA)	1	CTCTTGGGCTTTGGGACTTT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.005700	49763	50427	1186-1186	0.500000	60.0
trnT (UGU)-trnL (UAA)_p2	trnT (UGU)-trnL (UAA)	2	CTCTTGGGCTTTGGGACTTT	CACGCTTTCGGAAGTTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	5.1	0.019793	49763	50427	1438-1438	0.500000	59.8
trnT (UGU)-trnL (UAA)_p3	trnT (UGU)-trnL (UAA)	3	TATCCTCTTGGGCTTTGGGA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024814	49763	50427	1190-1190	0.500000	59.8
trnT (UGU)-trnL (UAA)_p4	trnT (UGU)-trnL (UAA)	4	TATCCTCTTGGGCTTTGGGA	CACGCTTTCGGAAGTTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	5.1	0.038908	49763	50427	1442-1442	0.500000	59.7
trnT (UGU)-trnL (UAA)_p5	trnT (UGU)-trnL (UAA)	5	CTCTTGGGCTTTGGGACTTT	TCACGCTTTCGGAAGTTTCA	20	20	50.0	45.0	58.0	58.1	0.0	5.1	0.058398	49763	50427	1439-1439	0.500000	59.5
cemA-pafII_p1	cemA-pafII	1	CGTCCAAGAGGAGAAGTTCG	TCTTTATGCTCAGCGTGTCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.026173	59248	60088	1202-1203	1.000000	88.7
cemA-pafII_p2	cemA-pafII	2	CGTCCAAGAGGAGAAGTTCG	CTTTATGCTCAGCGTGTCCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031763	59248	60088	1201-1202	1.000000	88.7
cemA-pafII_p3	cemA-pafII	3	CGTCCAAGAGGAGAAGTTCG	GGGATCGCGGGTCTTTTTAT	20	20	55.0	50.0	58.0	58.0	0.0	6.1	0.052856	59248	60088	1399-1400	1.000000	87.9
cemA-pafII_p4	cemA-pafII	4	CGTCCAAGAGGAGAAGTTCG	AAATGGAAACCGGAGCAAGT	20	20	55.0	45.0	58.0	58.0	0.0	9.5	0.020651	59248	60088	1627-1627	0.500000	59.8
cemA-pafII_p5	cemA-pafII	5	CGTCCAAGAGGAGAAGTTCG	ATTTCGTGGAATGTCGGGAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.040203	59248	60088	1358-1358	0.500000	59.7
trnA (UGC)-rrn23_p1	trnA (UGC)-rrn23	1	GGTTCGAGTCCGCTTATCTC	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	57.9	57.9	0.0	11.3	0.212163	93683	93830	216-216	1.000000	83.1
trnA (UGC)-rrn23_p2	trnA (UGC)-rrn23	2	GTTCGAGTCCGCTTATCTCC	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	57.9	57.9	0.0	11.3	0.212163	93683	93830	215-215	1.000000	83.1
trnA (UGC)-rrn23_p3	trnA (UGC)-rrn23	3	GGTTCGAGTCCGCTTATCTC	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	57.9	58.0	0.0	3.6	0.139468	93683	93830	288-288	1.000000	82.5
trnA (UGC)-rrn23_p4	trnA (UGC)-rrn23	4	GTTCGAGTCCGCTTATCTCC	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	57.9	58.0	0.0	3.6	0.139468	93683	93830	287-287	1.000000	82.5
trnA (UGC)-rrn23_p5	trnA (UGC)-rrn23	5	TTCGAGTCCGCTTATCTCCA	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.2	58.0	0.0	3.6	0.257969	93683	93830	286-286	1.000000	81.6
ycf1_p1	ycf1	1	CAGCGCTTCCTAGTTTAGCA	TTGCTCTGTCCGATTAAGCC	20	20	50.0	50.0	58.0	58.0	7.5	0.0	0.034321	99974	100093	881-881	0.500000	59.7
ycf1_p2	ycf1	2	ACAGCGCTTCCTAGTTTAGC	TTGCTCTGTCCGATTAAGCC	20	20	50.0	50.0	58.0	58.0	7.5	0.0	0.035716	99974	100093	882-882	0.500000	59.7
ycf1_p3	ycf1	3	CAGCGCTTCCTAGTTTAGCA	CCGATTAAGCCTTCTTCCCC	20	20	50.0	55.0	58.0	58.0	7.5	0.0	0.041441	99974	100093	872-872	0.500000	59.7
ycf1_p4	ycf1	4	CAGCGCTTCCTAGTTTAGCA	CGATTAAGCCTTCTTCCCCC	20	20	50.0	55.0	58.0	58.0	7.5	0.0	0.041441	99974	100093	871-871	0.500000	59.7
ycf1_p5	ycf1	5	ACAGCGCTTCCTAGTTTAGC	CGATTAAGCCTTCTTCCCCC	20	20	50.0	55.0	58.0	58.0	7.5	0.0	0.042836	99974	100093	872-872	0.500000	59.7
ORF441_p1	ORF441	1	ACTGCCGAGGAATATCCCTT	ACCACTGAGCTACTGAGGAA	20	20	50.0	50.0	58.2	57.7	0.0	11.2	0.502965	113666	114991	2008-2502	1.000000	88.0
ORF441_p2	ORF441	2	ACTGCCGAGGAATATCCCTT	CCTTCGGCCTTTTCGTTTTG	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.426117	113666	114991	2625-2625	0.500000	56.6
ORF441_p3	ORF441	3	ACTGCCGAGGAATATCCCTT	GAGGAACCTCAAAGTCGCTC	20	20	50.0	55.0	58.2	58.3	0.0	15.0	0.476193	113666	114991	2488-2488	0.500000	56.2
ORF441_p4	ORF441	4	ACTGCCGAGGAATATCCCTT	TGGAAAAGGCCATTTCAGCA	20	20	50.0	45.0	58.2	58.3	0.0	29.1	0.477646	113666	114991	1956-1956	0.500000	56.2
ORF441_p5	ORF441	5	TCGCATTTTTGATTTGCCGG	CCTTCGGCCTTTTCGTTTTG	20	20	45.0	50.0	58.3	58.2	0.0	0.0	0.526299	113666	114991	2677-2677	0.500000	55.8
rrn23-trnA (UGC)_p1	rrn23-trnA (UGC)	1	GTCTCTGGGTGCCTAGGTAT	GGTTCGAGTCCGCTTATCTC	20	20	55.0	55.0	57.9	57.9	11.3	0.0	0.212163	119307	119454	216-216	1.000000	83.1
rrn23-trnA (UGC)_p2	rrn23-trnA (UGC)	2	GTCTCTGGGTGCCTAGGTAT	GTTCGAGTCCGCTTATCTCC	20	20	55.0	55.0	57.9	57.9	11.3	0.0	0.212163	119307	119454	215-215	1.000000	83.1
rrn23-trnA (UGC)_p3	rrn23-trnA (UGC)	3	ATTCGGGAATCTCCGGATCT	GGTTCGAGTCCGCTTATCTC	20	20	50.0	55.0	58.0	57.9	3.6	0.0	0.139468	119307	119454	288-288	1.000000	82.5
rrn23-trnA (UGC)_p4	rrn23-trnA (UGC)	4	ATTCGGGAATCTCCGGATCT	GTTCGAGTCCGCTTATCTCC	20	20	50.0	55.0	58.0	57.9	3.6	0.0	0.139468	119307	119454	287-287	1.000000	82.5
rrn23-trnA (UGC)_p5	rrn23-trnA (UGC)	5	ATTCGGGAATCTCCGGATCT	TTCGAGTCCGCTTATCTCCA	20	20	50.0	50.0	58.0	58.2	3.6	0.0	0.257969	119307	119454	286-286	1.000000	81.6
ORF175_p1	ORF175	1	CTATCGAGTGCAGAGAGGGA	TCACTGGACTGTCTACGGTT	20	20	55.0	50.0	58.0	58.0	0.0	7.9	0.034614	134260	134787	1038-1038	0.500000	59.7
ORF175_p2	ORF175	2	CTATCGAGTGCAGAGAGGGA	TTCACTGGACTGTCTACGGT	20	20	55.0	50.0	58.0	58.0	0.0	7.9	0.034614	134260	134787	1039-1039	0.500000	59.7
ORF175_p3	ORF175	3	TCTATCGAGTGCAGAGAGGG	TCACTGGACTGTCTACGGTT	20	20	55.0	50.0	58.0	58.0	0.0	7.9	0.034614	134260	134787	1039-1039	0.500000	59.7
ORF175_p4	ORF175	4	TCTATCGAGTGCAGAGAGGG	TTCACTGGACTGTCTACGGT	20	20	55.0	50.0	58.0	58.0	0.0	7.9	0.034614	134260	134787	1040-1040	0.500000	59.7
ORF175_p5	ORF175	5	CTATCGAGTGCAGAGAGGGA	TTTGCATTCCGGCGAAAATC	20	20	55.0	45.0	58.0	58.0	0.0	9.3	0.036305	134260	134787	1180-1180	0.500000	59.7