primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ndhF	1	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	20	21	50.0	52.4	58.2	58.0	8.2	0.0	1.184325	108876	111110	2799-2832	0.500000	50.8
ndhF_p2	ndhF	2	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	20	21	45.0	52.4	57.8	58.0	0.0	0.0	1.193516	108876	111110	2809-2842	0.500000	50.7
ndhF_p3	ndhF	3	TCTCTTCCTTCTCCGAGCTC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	58.2	58.0	2.7	0.0	1.256922	108876	111110	2741-2774	0.500000	50.2
ndhF_p4	ndhF	4	TCGTTGTGGTCGGACTCTAT	ACGATTTTGAAAGGCCTCAGA	20	21	50.0	42.9	58.2	57.9	8.2	5.2	1.310046	108876	111110	2889-2922	0.500000	49.8
ndhF_p5	ndhF	5	ATCAATTCGGTCGTTGTGGT	ACGATTTTGAAAGGCCTCAGA	20	21	45.0	42.9	57.8	57.9	0.0	5.2	1.319237	108876	111110	2899-2932	0.500000	49.7
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CACCACCACAATGTGAGTGA	20	20	50.0	50.0	58.0	58.0	17.5	25.6	0.066479	111111	112151	1540-1547	0.500000	60.4
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CACCACCACAATGTGAGTGA	20	20	45.0	50.0	58.0	58.0	17.5	25.6	0.077769	111111	112151	1538-1545	0.500000	60.3
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CACCACCACAATGTGAGTGA	20	20	45.0	50.0	58.0	58.0	17.5	25.6	0.077769	111111	112151	1539-1546	0.500000	60.3
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	AATAAGACTCCCCACCACCA	20	20	50.0	50.0	58.0	57.9	17.5	0.0	0.086161	111111	112151	1552-1559	0.500000	60.3
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	AATAAGACTCCCCACCACCA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.097450	111111	112151	1550-1557	0.500000	60.2
rpl32_p1	rpl32	1	AGGTGTATTCTTTCCTCGAGT	CACCACCACAATGTGAGTGA	21	20	42.9	50.0	56.1	58.0	12.3	25.6	2.934472	112152	112316	852-858	0.500000	37.9
rpl32_p2	rpl32	2	AGGTGTATTCTTTCCTCGAGT	AATAAGACTCCCCACCACCA	21	20	42.9	50.0	56.1	57.9	12.3	0.0	2.954154	112152	112316	864-870	0.500000	37.8
rpl32_p3	rpl32	3	AGGTGTATTCTTTCCTCGAGT	TCTGCCCTTCTCGAGAAAGA	21	20	42.9	50.0	56.1	58.1	12.3	26.9	2.975676	112152	112316	920-926	0.500000	37.7
rpl32_p4	rpl32	4	AGGTGTATTCTTTCCTCGAGT	AGTTTCTTAGGGTGTCCGGT	21	20	42.9	50.0	56.1	58.3	12.3	0.0	3.174604	112152	112316	1341-1359	0.500000	37.0
rpl32_p5	rpl32	5	AGGTGTATTCTTTCCTCGAGTT	CACCACCACAATGTGAGTGA	22	20	40.9	50.0	56.8	58.0	12.3	25.6	3.194652	112152	112316	852-858	0.500000	35.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	112317	113168	984-1016	0.500000	59.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	112317	113168	985-1017	0.500000	59.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	112317	113168	967-999	0.500000	59.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	112317	113168	968-1000	0.500000	59.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGAAAGGATATTGGGCGG	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.464114	112317	113168	1638-1671	0.500000	58.1
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TCACTCACATTGTGGTGGTG	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.0	57.9	25.1	0.0	0.133207	113249	113389	1313-1345	0.500000	60.8
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TCTTTCTCGAGAAGGGCAGA	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.1	57.9	30.1	0.0	0.174410	113249	113389	1245-1277	0.500000	60.4
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTGAAATCGGTAGACACGCT	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106972	113249	113389	674-680	0.500000	59.5
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TGAAATCGGTAGACACGCTG	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.108342	113249	113389	673-679	0.500000	59.5
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TGGTGGTGGGGAGTCTTATT	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.152888	113249	113389	1301-1320	0.333333	53.6
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	AGAACCGCGTGAATCACTAC	20	20	50.0	50.0	58.0	58.0	0.0	11.9	0.015661	113390	114358	1296-1302	0.500000	60.2
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	AGAACCGCGTGAATCACTAC	20	20	50.0	50.0	58.0	58.0	0.0	11.9	0.017031	113390	114358	1295-1301	0.500000	60.2
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	113390	114358	1571-1578	0.500000	60.1
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	113390	114358	1570-1577	0.500000	60.0
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	TAGCTTTTCGATGGCTTCCC	20	20	50.0	50.0	58.0	58.0	0.0	11.4	0.046217	113390	114358	1794-1801	0.500000	60.0
ndhD_p1	ndhD	1	GTAGTGATTCACGCGGTTCT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.0	58.0	12.5	0.0	0.023287	114605	116134	1899-1900	0.500000	60.4
ndhD_p2	ndhD	2	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	12.5	0.0	0.102592	114605	116134	2121-2122	0.500000	59.7
ndhD_p3	ndhD	3	GTAGTGATTCACGCGGTTCT	TGATACCTTGGGACGGATGT	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.133701	114605	116134	1937-1938	0.500000	59.5
ndhD_p4	ndhD	4	GTAGTGATTCACGCGGTTCT	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	12.5	0.0	0.149365	114605	116134	2117-2118	0.500000	59.3
ndhD_p5	ndhD	5	GTAGTGATTCACGCGGTTCT	CAGAGGATTGTGTCGGTTGT	20	20	50.0	50.0	58.0	57.8	12.5	0.0	0.177663	114605	116134	1886-1887	0.500000	59.2
psaC_p1	psaC	1	TCTTTCCTCCCCACATGGAT	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.109141	116227	116472	942-942	0.500000	59.6
psaC_p2	psaC	2	CTCCCGCCGTGTACAAAATA	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107699	116227	116472	987-987	0.500000	59.5
psaC_p3	psaC	3	CTCCCGCCGTGTACAAAATA	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.154472	116227	116472	983-983	0.500000	59.2
psaC_p4	psaC	4	TCTTTCCTCCCCACATGGAT	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.155914	116227	116472	938-938	0.500000	59.2
psaC_p5	psaC	5	TTCCAAAGCCACAGGATACG	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.202712	116227	116472	1097-1097	0.500000	58.9
ndhE_p1	ndhE	1	TCCTCTGTTCTTGGAGCAGA	ATTCTGCCTTTTCGTTGGGA	20	20	50.0	45.0	58.0	57.7	12.1	0.0	0.301821	116709	117011	1328-1330	0.500000	57.8
ndhE_p2	ndhE	2	TCCTCTGTTCTTGGAGCAGA	TCTGCCTTTTCGTTGGGATT	20	20	50.0	45.0	58.0	57.7	12.1	0.0	0.301821	116709	117011	1326-1328	0.500000	57.8
ndhE_p3	ndhE	3	TCCTCTGTTCTTGGAGCAGA	TTCTGCCTTTTCGTTGGGAT	20	20	50.0	45.0	58.0	57.7	12.1	0.0	0.301821	116709	117011	1327-1329	0.500000	57.8
ndhE_p4	ndhE	4	TCCTCTGTTCTTGGAGCAGA	TAGGGGGTCTGGGAGTAGTA	20	20	50.0	55.0	58.0	57.7	12.1	0.0	0.324178	116709	117011	1367-1369	0.500000	57.7
ndhE_p5	ndhE	5	TCCTCTGTTCTTGGAGCAGA	TAATCGGCGCAATTGCTGTA	20	20	50.0	45.0	58.0	58.3	12.1	26.9	0.357634	116709	117011	929-931	0.500000	57.5
ndhE-ndhG_p1	ndhE-ndhG	1	TCCTCTGTTCTTGGAGCAGA	ATTCTGCCTTTTCGTTGGGA	20	20	50.0	45.0	58.0	57.7	12.1	0.0	0.301821	117012	117231	1328-1330	0.500000	57.8
ndhE-ndhG_p2	ndhE-ndhG	2	TCCTCTGTTCTTGGAGCAGA	TCTGCCTTTTCGTTGGGATT	20	20	50.0	45.0	58.0	57.7	12.1	0.0	0.301821	117012	117231	1326-1328	0.500000	57.8
ndhE-ndhG_p3	ndhE-ndhG	3	TCCTCTGTTCTTGGAGCAGA	TTCTGCCTTTTCGTTGGGAT	20	20	50.0	45.0	58.0	57.7	12.1	0.0	0.301821	117012	117231	1327-1329	0.500000	57.8
ndhE-ndhG_p4	ndhE-ndhG	4	TCCTCTGTTCTTGGAGCAGA	TAGGGGGTCTGGGAGTAGTA	20	20	50.0	55.0	58.0	57.7	12.1	0.0	0.324178	117012	117231	1367-1369	0.500000	57.7
ndhE-ndhG_p5	ndhE-ndhG	5	TCCTCTGTTCTTGGAGCAGA	TAATCGGCGCAATTGCTGTA	20	20	50.0	45.0	58.0	58.3	12.1	26.9	0.357634	117012	117231	929-931	0.500000	57.5
ndhG_p1	ndhG	1	CGATGAGTTCCCCGAGTTTT	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.188068	117232	117762	1908-1911	0.500000	58.8
ndhG_p2	ndhG	2	GAATCGATGAGTTCCCCGAG	TTGATCACATCCGAGCGTTT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.234841	117232	117762	1912-1915	0.500000	58.5
ndhG_p3	ndhG	3	CGATGAGTTCCCCGAGTTTT	TCAAATTGCTTTGGGTCGGT	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.396256	117232	117762	1633-1636	0.500000	57.2
ndhG_p4	ndhG	4	CGATGAGTTCCCCGAGTTTT	TGAGGGTTTTTGCTTGGTGA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.246264	117232	117762	1509-1509	0.333333	51.5
ndhG_p5	ndhG	5	GAATCGATGAGTTCCCCGAG	TGAGGGTTTTTGCTTGGTGA	20	20	55.0	45.0	57.9	57.8	0.0	0.0	0.293037	117232	117762	1513-1513	0.333333	51.1
ndhI_p1	ndhI	1	GCCGTACCATGACGTATCTG	AGCCGAGTGTGAACGATTAC	20	20	55.0	50.0	58.2	58.0	6.2	0.0	0.240542	118103	118603	1708-1709	0.500000	58.3
ndhI_p2	ndhI	2	TCACCAAGCAAAAACCCTCA	AGCCGAGTGTGAACGATTAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.159333	118103	118603	1062-1062	0.333333	52.1
ndhI_p3	ndhI	3	TCACCAAGCAAAAACCCTCA	TTTGTGACCTTTGAGGAGCC	20	20	45.0	50.0	57.8	58.0	0.0	0.1	0.170246	118103	118603	1268-1268	0.333333	52.0
ndhI_p4	ndhI	4	TCACCAAGCAAAAACCCTCA	GATGGACTTTACCGAGGCTG	20	20	45.0	55.0	57.8	58.1	0.0	0.0	0.210177	118103	118603	757-757	0.333333	51.8
ndhI_p5	ndhI	5	TCACCAAGCAAAAACCCTCA	GACCTTTGAGGAGCCGTATG	20	20	45.0	55.0	57.8	58.1	0.0	0.1	0.210177	118103	118603	1263-1263	0.333333	51.7
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	118682	120834	2734-2739	0.500000	59.4
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	118682	120834	2735-2740	0.500000	59.4
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	TTATGCGAGAGTCGAAGCAC	20	20	50.0	50.0	58.0	58.1	0.0	11.3	0.128831	118682	120834	2508-2513	0.500000	59.2
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	TGGGAAGTCCAATGGCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.132307	118682	120834	2747-2752	0.500000	59.1
ndhA_p5	ndhA	5	AAACGCTCGGATGTGATCAA	GGCAAAAAGAGGGGGATTCA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.156115	118682	120834	2821-2826	0.500000	58.9
ndhH_p1	ndhH	1	CGTTGTTGTATACCCGCAGA	TCAACGGTTGCTGGCTTATT	20	20	50.0	45.0	58.0	58.1	0.0	15.9	0.096665	120836	122017	1550-1550	0.500000	59.4
ndhH_p2	ndhH	2	CGTTGTTGTATACCCGCAGA	CAGATGGACATATGCGCTGT	20	20	50.0	50.0	58.0	58.1	0.0	9.7	0.125943	120836	122017	2045-2061	0.500000	59.3
ndhH_p3	ndhH	3	TGCGTATTCAGGGCCAATAC	TCAACGGTTGCTGGCTTATT	20	20	50.0	45.0	58.0	58.1	6.2	15.9	0.133920	120836	122017	1569-1569	0.500000	59.1
ndhH_p4	ndhH	4	CCTGACATAAGAAGGCCGAC	TCAACGGTTGCTGGCTTATT	20	20	55.0	45.0	58.1	58.1	0.0	15.9	0.154388	120836	122017	1814-1814	0.500000	58.9
ndhH_p5	ndhH	5	GACATAAGAAGGCCGACAGG	TCAACGGTTGCTGGCTTATT	20	20	55.0	45.0	58.1	58.1	0.0	15.9	0.154388	120836	122017	1811-1811	0.500000	58.9
rps15_p1	rps15	1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.0	58.0	26.1	0.0	0.040065	122109	122387	1044-1060	0.500000	60.3
rps15_p2	rps15	2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.085751	122109	122387	907-923	0.500000	59.9
rps15_p3	rps15	3	GCAGTGACTCCTCCAATACG	AATAATGGGAGCCGCTTGAG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.099233	122109	122387	1326-1342	0.500000	59.7
rps15_p4	rps15	4	AGGAGTCTGTGCCCCAATAT	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.128265	122109	122387	1222-1238	0.500000	59.5
rps15_p5	rps15	5	TCCCAACGCGTTACATAAGG	CAGATGGACATATGCGCTGT	20	20	50.0	50.0	58.0	58.1	26.1	9.7	0.137928	122109	122387	892-908	0.500000	59.4
rps15-ycf1_p1	rps15-ycf1	1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.0	58.0	26.1	0.0	0.040065	122388	122801	1044-1060	0.500000	60.3
rps15-ycf1_p2	rps15-ycf1	2	CCGCAGACCTCTCTGAGATA	AATAATGGGAGCCGCTTGAG	20	20	55.0	50.0	58.0	58.0	8.0	0.0	0.052863	122388	122801	630-646	0.500000	60.3
rps15-ycf1_p3	rps15-ycf1	3	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.085751	122388	122801	907-923	0.500000	59.9
rps15-ycf1_p4	rps15-ycf1	4	AATAAGCCAGCAACCGTTGA	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	15.6	0.0	0.122841	122388	122801	667-683	0.500000	59.7
rps15-ycf1_p5	rps15-ycf1	5	CCAGAATTTTCCGCAGACCT	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.200743	122388	122801	640-656	0.500000	59.1
ycf1_p1	ycf1	1	CTCAAGCGGCTCCCATTATT	CCTTGGTTTATCCCGAGCAA	20	20	50.0	50.0	58.0	57.9	1.4	0.0	0.149911	123242	123361	956-1010	0.500000	61.4
ycf1_p2	ycf1	2	GCGGCTCCCATTATTGGTAT	CCTTGGTTTATCCCGAGCAA	20	20	50.0	50.0	57.8	57.9	3.1	0.0	0.323156	123242	123361	951-1005	0.500000	60.0
ycf1_p3	ycf1	3	CTCAAGCGGCTCCCATTATT	TACGGTCTCCTGCTTAACCT	20	20	50.0	50.0	58.0	57.8	1.4	0.0	0.254090	123242	123361	623-623	0.500000	58.5
ycf1_p4	ycf1	4	CTCAAGCGGCTCCCATTATT	AACCTTGGTTTATCCCGAGC	20	20	50.0	50.0	58.0	57.9	1.4	0.0	0.151405	123242	123361	958-982	0.333333	54.4
ycf1_p5	ycf1	5	GCGGCTCCCATTATTGGTAT	AACCTTGGTTTATCCCGAGC	20	20	50.0	50.0	57.8	57.9	3.1	0.0	0.324650	123242	123361	953-977	0.333333	53.1