primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	CCATGGGTGACACAGATCAG	TAGCCACAGGGTATACGCTT	20	20	55.0	50.0	58.0	58.2	2.8	19.1	0.249746	3740	3859	205-205	1.000000	79.6
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	CCATGGGTGACACAGATCAG	GCCACAGGGTATACGCTTTT	20	20	55.0	50.0	58.0	58.3	2.8	19.1	0.287634	3740	3859	203-203	1.000000	79.3
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GACACAGATCAGCCGAAGAG	TAGCCACAGGGTATACGCTT	20	20	55.0	50.0	58.1	58.2	0.0	19.1	0.295724	3740	3859	197-197	1.000000	79.3
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GACACAGATCAGCCGAAGAG	GCCACAGGGTATACGCTTTT	20	20	55.0	50.0	58.1	58.3	0.0	19.1	0.333612	3740	3859	195-195	1.000000	79.0
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	ACAGATCAGCCGAAGAGAGT	TAGCCACAGGGTATACGCTT	20	20	50.0	50.0	58.2	58.2	0.0	19.1	0.369635	3740	3859	194-194	1.000000	78.7
matK-rps16_p1	matK-rps16	1	AGCATTCCCATTTTGGATCGA	CTGCCATCATTTCCTTCCCA	21	20	42.9	50.0	58.0	57.9	0.0	0.0	1.165494	17044	18160	1303-1303	1.000000	71.1
matK-rps16_p2	matK-rps16	2	AGCATTCCCATTTTGGATCGA	TGCCATCATTTCCTTCCCAG	21	20	42.9	50.0	58.0	57.9	0.0	0.0	1.165494	17044	18160	1302-1302	1.000000	71.1
matK-rps16_p3	matK-rps16	3	AGCATTCCCATTTTGGATCGA	GGGCTCAAACCACAGAAACT	21	20	42.9	50.0	58.0	58.3	0.0	0.0	1.324637	17044	18160	1276-1276	1.000000	69.8
matK-rps16_p4	matK-rps16	4	AGCATTCCCATTTTGGATCGA	GCCATCATTTCCTTCCCAGA	21	20	42.9	50.0	58.0	57.6	0.0	0.0	1.459285	17044	18160	1301-1301	1.000000	68.8
matK-rps16_p5	matK-rps16	5	AGCATTCCCATTTTGGATCGA	TCTGCCATCATTTCCTTCCC	21	20	42.9	50.0	58.0	57.6	0.0	0.0	1.459285	17044	18160	1304-1304	1.000000	68.8
rps16-chlB_p1	rps16-chlB	1	GATTTGGGGATGGCGGTTAT	CCGGTTTTGTGAGGGGAAAA	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.599781	19281	19461	395-413	1.000000	76.2
rps16-chlB_p2	rps16-chlB	2	ACGTCGCTTTCCACCATATC	CCGGTTTTGTGAGGGGAAAA	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.659225	19281	19461	339-357	1.000000	75.9
rps16-chlB_p3	rps16-chlB	3	GTCGCTTTCCACCATATCGT	CCGGTTTTGTGAGGGGAAAA	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.659225	19281	19461	337-355	1.000000	75.9
rps16-chlB_p4	rps16-chlB	4	TCTCGATGTTTGAGCCACAC	CCGGTTTTGTGAGGGGAAAA	20	20	50.0	50.0	58.2	58.6	0.0	0.0	0.799390	19281	19461	357-375	1.000000	74.7
rps16-chlB_p5	rps16-chlB	5	ACTCCGCAAGAATGATCTCG	CCGGTTTTGTGAGGGGAAAA	20	20	50.0	50.0	57.8	58.6	0.0	0.0	0.811065	19281	19461	372-390	1.000000	74.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGGAGAAGCTGTGGGGATTA	TAGAAGTGCTTCAACGAGCC	20	20	50.0	50.0	58.0	57.9	0.0	13.6	0.114872	44088	44749	825-826	1.000000	80.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ATTGTTTTGAGCGACCGTCT	TAGAAGTGCTTCAACGAGCC	20	20	45.0	50.0	58.1	57.9	0.0	13.6	0.201333	44088	44749	861-862	1.000000	79.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TTTTGAGCGACCGTCTGAAT	TAGAAGTGCTTCAACGAGCC	20	20	45.0	50.0	57.8	57.9	0.0	13.6	0.244104	44088	44749	857-858	1.000000	78.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GAGCGACCGTCTGAATGTAA	TAGAAGTGCTTCAACGAGCC	20	20	50.0	50.0	57.7	57.9	0.0	13.6	0.353656	44088	44749	853-854	1.000000	78.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TTTGAGCGACCGTCTGAATG	TAGAAGTGCTTCAACGAGCC	20	20	50.0	50.0	58.6	57.9	0.0	13.6	0.651838	44088	44749	856-857	1.000000	75.6
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	ATGGTCGAGTGGTTTATGGC	CCTATGGCATGCAGGAAGAG	20	20	50.0	55.0	58.0	58.1	0.0	21.4	0.144842	46669	47486	996-1001	1.000000	79.6
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	TTCGAATCCCTCTCTCTCCC	CCTATGGCATGCAGGAAGAG	20	20	55.0	55.0	57.9	58.1	0.0	21.4	0.181184	46669	47486	933-938	1.000000	79.4
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	AGTGCTGAAACGCTATCGAG	CCTATGGCATGCAGGAAGAG	20	20	50.0	55.0	58.1	58.1	0.0	21.4	0.191822	46669	47486	955-960	1.000000	79.3
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	ATGGTCGAGTGGTTTATGGC	CTTCTTCTTCGTGGGTCACC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.241362	46669	47486	1014-1019	1.000000	78.9
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	ATGGTCGAGTGGTTTATGGC	AGCCGGGTTTGAAAAAGGAA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.255316	46669	47486	963-968	1.000000	78.8
rps4-trnL-UAA_p1	rps4-trnL-UAA	1	AGCCCTGGTAAATCTTTCAGAC	GAGCCATCACTCGATGTGTT	22	20	45.5	50.0	58.0	58.0	0.0	12.6	2.058992	60748	61705	1084-1103	1.000000	67.7
rps4-trnL-UAA_p2	rps4-trnL-UAA	2	TGTTTTGTTAGTAAGCCCTGGT	GAGCCATCACTCGATGTGTT	22	20	40.9	50.0	57.8	58.0	0.0	12.6	2.189026	60748	61705	1097-1116	1.000000	66.6
rps4-trnL-UAA_p3	rps4-trnL-UAA	3	AGCCCTGGTAAATCTTTCAGAC	CCTGATTTTGGGAGCCATCA	22	20	45.5	50.0	58.0	57.9	0.0	0.0	2.191702	60748	61705	1095-1114	1.000000	66.6
rps4-trnL-UAA_p4	rps4-trnL-UAA	4	CCCTGGTAAATCTTTCAGACGA	GAGCCATCACTCGATGTGTT	22	20	45.5	50.0	57.8	58.0	0.0	12.6	2.217981	60748	61705	1082-1101	1.000000	66.4
rps4-trnL-UAA_p5	rps4-trnL-UAA	5	TGGTAAATCTTTCAGACGACGT	GAGCCATCACTCGATGTGTT	22	20	40.9	50.0	57.8	58.0	0.0	12.6	2.263003	60748	61705	1079-1098	1.000000	66.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	AGGCAAGGAATGAGGCTAGA	GCATGTTGGGTTCGTAAAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.137763	64710	65392	843-843	1.000000	79.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AGGCAAGGAATGAGGCTAGA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.190930	64710	65392	773-773	1.000000	79.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AGGCAAGGAATGAGGCTAGA	ATATTCCGCTCTGCATGACC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.290829	64710	65392	805-805	1.000000	78.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AGGCAAGGAATGAGGCTAGA	CCGAGAATGTCTACGGTTCG	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.291123	64710	65392	787-787	1.000000	78.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AGGCAAGGAATGAGGCTAGA	TCTGCATGACCGAGAATGTC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.414944	64710	65392	796-796	1.000000	77.5
accD-psaI_p1	accD-psaI	1	TCCACGTAATCTGATGAAAGGT	CCGAACCTACTAGGGGTACA	22	20	40.9	55.0	57.2	57.6	0.0	7.1	3.261341	71919	72507	711-714	1.000000	55.1
accD-psaI_p2	accD-psaI	2	TCCACGTAATCTGATGAAAGGT	ACCGAACCTACTAGGGGTAC	22	20	40.9	55.0	57.2	57.6	0.0	7.1	3.263063	71919	72507	712-715	1.000000	55.1
accD-psaI_p3	accD-psaI	3	CCACGTAATCTGATGAAAGGTG	CCGAACCTACTAGGGGTACA	22	20	45.5	55.0	57.1	57.6	11.0	7.1	3.307152	71919	72507	710-713	1.000000	54.8
accD-psaI_p4	accD-psaI	4	CCACGTAATCTGATGAAAGGTG	ACCGAACCTACTAGGGGTAC	22	20	45.5	55.0	57.1	57.6	11.0	7.1	3.308874	71919	72507	711-714	1.000000	54.8
accD-psaI_p5	accD-psaI	5	TTCCACGTAATCTGATGAAAGGT	CCGAACCTACTAGGGGTACA	23	20	39.1	55.0	57.8	57.6	0.0	7.1	3.602844	71919	72507	712-715	1.000000	52.4
psbE-petL_p1	psbE-petL	1	AATGTCAGCAAAGGGACGTT	ACGTCATAGAAGCTGGATCT	20	20	45.0	45.0	58.0	55.8	0.0	0.0	2.265772	78315	79480	1304-1310	1.000000	62.7
psbE-petL_p2	psbE-petL	2	GGACGTTCTCCCGTATTTCC	ACGTCATAGAAGCTGGATCT	20	20	55.0	45.0	58.1	55.8	0.0	0.0	2.315123	78315	79480	1291-1297	1.000000	62.3
psbE-petL_p3	psbE-petL	3	GCTATGGATGACCCGATGTC	ACGTCATAGAAGCTGGATCT	20	20	55.0	45.0	57.9	55.8	0.0	0.0	2.333083	78315	79480	1337-1343	1.000000	62.1
psbE-petL_p4	psbE-petL	4	AATGTCAGCAAAGGGACGTT	ACGTCATAGAAGCTGGATCTT	20	21	45.0	42.9	58.0	56.5	0.0	0.0	2.479043	78315	79480	1304-1310	1.000000	61.0
psbE-petL_p5	psbE-petL	5	CCAGCAATGAACAGAGAGGG	ACGTCATAGAAGCTGGATCT	20	20	55.0	45.0	58.3	55.8	0.0	0.0	2.499319	78315	79480	1366-1372	1.000000	60.8
rpl20-rps12_p1	rpl20-rps12	1	TGTTGGTTCGCTGTTCTGAA	TGGACATGCCAACCAATCAA	20	20	45.0	45.0	58.0	58.0	0.0	6.4	0.038064	82044	82959	1148-1148	1.000000	80.0
rpl20-rps12_p2	rpl20-rps12	2	TTGTTGGTTCGCTGTTCTGA	TGGACATGCCAACCAATCAA	20	20	45.0	45.0	58.0	58.0	0.0	6.4	0.038064	82044	82959	1149-1149	1.000000	80.0
rpl20-rps12_p3	rpl20-rps12	3	CGCTGTTCTGAACGATTTCG	TGGACATGCCAACCAATCAA	20	20	50.0	45.0	57.9	58.0	15.2	6.4	0.137620	82044	82959	1140-1140	1.000000	79.2
rpl20-rps12_p4	rpl20-rps12	4	TGTTGGTTCGCTGTTCTGAA	AGGCAAGACAACCGATGAAG	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.217745	82044	82959	1115-1115	1.000000	78.5
rpl20-rps12_p5	rpl20-rps12	5	TTGTTGGTTCGCTGTTCTGA	AGGCAAGACAACCGATGAAG	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.217745	82044	82959	1116-1116	1.000000	78.5
rps12-clpP_p1	rps12-clpP	1	CTTCATCGGTTGTCTTGCCT	GGGGGAATGTATCACGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	0.0	0.442604	83074	83597	637-642	1.000000	77.6
rps12-clpP_p2	rps12-clpP	2	CTTCATCGGTTGTCTTGCCT	GGGAATGTATCACGGAAGCA	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.512070	83074	83597	635-640	1.000000	77.0
rps12-clpP_p3	rps12-clpP	3	TCATCGGTTGTCTTGCCTTT	GGGGGAATGTATCACGGAAG	20	20	45.0	55.0	57.7	57.7	0.0	0.0	0.526469	83074	83597	635-640	1.000000	76.9
rps12-clpP_p4	rps12-clpP	4	TTCATCGGTTGTCTTGCCTT	GGGGGAATGTATCACGGAAG	20	20	45.0	55.0	57.7	57.7	0.0	0.0	0.526469	83074	83597	636-641	1.000000	76.9
rps12-clpP_p5	rps12-clpP	5	CTTCATCGGTTGTCTTGCCT	GGGGAATGTATCACGGAAGC	20	20	50.0	55.0	58.2	58.4	0.0	0.0	0.592156	83074	83597	636-641	1.000000	76.4
petB-petD_p1	petB-petD	1	GCTCCTTACGGCCGTTTTTA	GCGGTATTCGATGGGGTATC	20	20	50.0	55.0	58.6	58.0	38.9	0.0	0.564233	89830	90096	405-409	1.000000	77.1
petB-petD_p2	petB-petD	2	GCTCCTTACGGCCGTTTTTA	TCGATGGGGTATCTGCATCT	20	20	50.0	50.0	58.6	58.0	38.9	0.0	0.573719	89830	90096	398-402	1.000000	77.0
petB-petD_p3	petB-petD	3	GCTCCTTACGGCCGTTTTTA	CGATGGGGTATCTGCATCTG	20	20	50.0	55.0	58.6	57.9	38.9	0.0	0.648662	89830	90096	397-401	1.000000	76.4
petB-petD_p4	petB-petD	4	GCTCCTTACGGCCGTTTTTA	GCATCTGCATAGCCTACGTT	20	20	50.0	50.0	58.6	58.1	38.9	0.0	0.687182	89830	90096	384-388	1.000000	76.2
petB-petD_p5	petB-petD	5	GCTCCTTACGGCCGTTTTTA	AGATGTGGCGGTATTCGATG	20	20	50.0	50.0	58.6	57.8	38.9	0.0	0.713116	89830	90096	412-416	1.000000	75.8
rps8-rpl14_p1	rps8-rpl14	1	CGGGTATTCGAACCGTAGTC	TCTTCGGTCCAGTAGCTAGG	20	20	55.0	55.0	57.9	57.9	15.4	0.0	0.165273	94083	94518	587-595	1.000000	81.2
rps8-rpl14_p2	rps8-rpl14	2	CCCGTACTGGCATTTCGTAT	TCTTCGGTCCAGTAGCTAGG	20	20	50.0	55.0	57.8	57.9	3.5	0.0	0.288532	94083	94518	561-569	1.000000	80.4
rps8-rpl14_p3	rps8-rpl14	3	CGGGTATTCGAACCGTAGTC	CCAGAGTCTTCGGTCCAGTA	20	20	55.0	55.0	57.9	58.2	15.4	0.0	0.275546	94083	94518	593-601	1.000000	80.3
rps8-rpl14_p4	rps8-rpl14	4	GTCTTTCTTCCCGTACTGGC	TCTTCGGTCCAGTAGCTAGG	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.331013	94083	94518	570-578	1.000000	80.0
rps8-rpl14_p5	rps8-rpl14	5	CGGGTATTCGAACCGTAGTC	TGTGGTGAATCAGGAGGGAA	20	20	55.0	50.0	57.9	58.3	15.4	0.0	0.370013	94083	94518	625-633	1.000000	79.5
trnI-CAU-rrn16_p1	trnI-CAU-rrn16	1	GTTAGGCGGGATGTTCTGAG	GCTGCTACACGTCAATACCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.063258	104619	104768	1201-1318	1.000000	85.4
trnI-CAU-rrn16_p2	trnI-CAU-rrn16	2	GTTAGGCGGGATGTTCTGAG	TGCTGCTACACGTCAATACC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.063258	104619	104768	1202-1319	1.000000	85.4
trnI-CAU-rrn16_p3	trnI-CAU-rrn16	3	ATTGAACTAGGGCTGGGACT	GCTGCTACACGTCAATACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031995	104619	104768	1411-1555	1.000000	84.8
trnI-CAU-rrn16_p4	trnI-CAU-rrn16	4	ATTGAACTAGGGCTGGGACT	TGCTGCTACACGTCAATACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031995	104619	104768	1412-1556	1.000000	84.8
trnI-CAU-rrn16_p5	trnI-CAU-rrn16	5	TTAGGCGGGATGTTCTGAGA	GCTGCTACACGTCAATACCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.143942	104619	104768	1200-1317	1.000000	84.8
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	TCTTCTTTAGCCCGCTCAAC	TAACGAACGGGATATAGCGC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.431317	117430	118558	1297-1303	1.000000	77.2
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	CTTCTTTAGCCCGCTCAACA	TAACGAACGGGATATAGCGC	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.528144	117430	118558	1296-1302	1.000000	76.4
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	TTTAGCCCGCTCAACATTGA	TAACGAACGGGATATAGCGC	20	20	45.0	50.0	57.8	57.7	0.0	0.0	0.529218	117430	118558	1292-1298	1.000000	76.4
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	CTTCTTCTTTAGCCCGCTCA	TAACGAACGGGATATAGCGC	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.723215	117430	118558	1299-1305	1.000000	74.8
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	CCGGTCGTTCCAGAAGTTTT	TAACGAACGGGATATAGCGC	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.746752	117430	118558	1272-1278	1.000000	74.7
ycf1-chlN_p1	ycf1-chlN	1	TACAACGACATTCGAACGCT	TGGATTTACGAACGCGAGAG	20	20	45.0	50.0	57.9	58.1	0.0	15.9	0.150543	133690	133911	365-369	1.000000	80.3
ycf1-chlN_p2	ycf1-chlN	2	TACAACGACATTCGAACGCT	TTACGAACGCGAGAGATGTG	20	20	45.0	50.0	57.9	58.1	0.0	15.9	0.163509	133690	133911	360-364	1.000000	80.2
ycf1-chlN_p3	ycf1-chlN	3	AATAGTAGGGGAGGACCTGC	TGGATTTACGAACGCGAGAG	20	20	55.0	50.0	57.9	58.1	4.0	15.9	0.200525	133690	133911	422-426	1.000000	79.7
ycf1-chlN_p4	ycf1-chlN	4	AATAGTAGGGGAGGACCTGC	TTACGAACGCGAGAGATGTG	20	20	55.0	50.0	57.9	58.1	4.0	15.9	0.213491	133690	133911	417-421	1.000000	79.6
ycf1-chlN_p5	ycf1-chlN	5	TACAACGACATTCGAACGCT	GGATTTACGAACGCGAGAGA	20	20	45.0	50.0	57.9	57.8	0.0	15.9	0.239072	133690	133911	364-368	1.000000	79.6
rrn16-ndhB_p1	rrn16-ndhB	1	GCTGCTACACGTCAATACCA	GTTAGGCGGGATGTTCTGAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.063258	145803	145952	1201-1318	1.000000	85.4
rrn16-ndhB_p2	rrn16-ndhB	2	TGCTGCTACACGTCAATACC	GTTAGGCGGGATGTTCTGAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.063258	145803	145952	1202-1319	1.000000	85.4
rrn16-ndhB_p3	rrn16-ndhB	3	GCTGCTACACGTCAATACCA	ATTGAACTAGGGCTGGGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031995	145803	145952	1411-1555	1.000000	84.8
rrn16-ndhB_p4	rrn16-ndhB	4	TGCTGCTACACGTCAATACC	ATTGAACTAGGGCTGGGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031995	145803	145952	1412-1556	1.000000	84.8
rrn16-ndhB_p5	rrn16-ndhB	5	GCTGCTACACGTCAATACCA	TTAGGCGGGATGTTCTGAGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.143942	145803	145952	1200-1317	1.000000	84.8