primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CTCTAGACCTAGCCGCTCTT	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.153260	83	441	424-425	1.000000	79.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CTCTAGACCTAGCCGCTCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.157622	83	441	438-439	1.000000	78.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CTCTAGACCTAGCCGCTCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.159129	83	441	437-438	1.000000	78.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CTCTAGACCTAGCCGCTCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.159129	83	441	439-440	1.000000	78.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TAGACCTAGCCGCTCTTGAA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.268243	83	441	421-422	1.000000	78.0
rps16_p1	rps16	1	ACGAATAGAACAAGAAAAAGGGA	TGTACCGACTGAACCAATGAC	23	21	34.8	47.6	56.1	58.0	0.0	0.0	5.909018	5158	6278	1311-1317	1.000000	40.5
rps16_p2	rps16	2	ACGAATAGAACAAGAAAAAGGGA	GTACCGACTGAACCAATGACT	23	21	34.8	47.6	56.1	57.7	0.0	0.0	6.188839	5158	6278	1310-1316	1.000000	40.5
rps16_p3	rps16	3	ACGAATAGAACAAGAAAAAGGGAA	TGTACCGACTGAACCAATGAC	24	21	33.3	47.6	56.8	58.0	0.0	0.0	6.233275	5158	6278	1311-1317	1.000000	40.5
rps16_p4	rps16	4	ACGAATAGAACAAGAAAAAGGGA	TCAATTCCATACGGGTTCCA	23	20	34.8	45.0	56.1	56.5	0.0	0.0	6.353489	5158	6278	1268-1274	1.000000	40.5
rps16_p5	rps16	5	ACGAATAGAACAAGAAAAAGGG	TGTACCGACTGAACCAATGAC	22	21	36.4	47.6	54.7	58.0	0.0	0.0	6.365572	5158	6278	1311-1317	1.000000	40.5
trnG-GCC_p1	trnG-GCC	1	GCGAAAAACGTCGACACTTT	TGTGTGAGAAAATTTCGGGA	20	20	45.0	40.0	57.9	55.2	17.2	0.0	2.883863	10155	10927	986-987	1.000000	57.0
trnG-GCC_p2	trnG-GCC	2	CTCTCTTCGACAAAAAGCGC	TGTGTGAGAAAATTTCGGGA	20	20	50.0	40.0	57.8	55.2	0.0	0.0	3.021360	10155	10927	920-921	1.000000	55.9
trnG-GCC_p3	trnG-GCC	3	GCGAAAAACGTCGACACTTT	TGTGTGAGAAAATTTCGGGAA	20	21	45.0	38.1	57.9	56.0	17.2	0.0	3.074655	10155	10927	986-987	1.000000	55.5
trnG-GCC_p4	trnG-GCC	4	GCGAAAAACGTCGACACTTT	TTGTGTGAGAAAATTTCGGGA	20	21	45.0	38.1	57.9	56.0	17.2	0.0	3.074655	10155	10927	987-988	1.000000	55.5
trnG-GCC_p5	trnG-GCC	5	CTCTCTTCGACAAAAAGCGC	TGTGTGAGAAAATTTCGGGAA	20	21	50.0	38.1	57.8	56.0	0.0	0.0	3.212152	10155	10927	920-921	1.000000	54.4
atpA-atpF_p1	atpA-atpF	1	ATGCCATCGCCTACTTGAAG	ATATTGACATGTTGGGGGCG	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.349762	12808	12862	211-212	1.000000	77.6
atpA-atpF_p2	atpA-atpF	2	ATGCCATCGCCTACTTGAAG	GGGGGCGATGAACGAAATAA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.370168	12808	12862	198-199	1.000000	77.4
atpA-atpF_p3	atpA-atpF	3	ATGCCATCGCCTACTTGAAG	GGCGATGAACGAAATAACCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.403626	12808	12862	195-196	1.000000	77.2
atpA-atpF_p4	atpA-atpF	4	CAATGCCATCGCCTACTTGA	ATATTGACATGTTGGGGGCG	20	20	50.0	50.0	58.3	58.3	0.0	0.0	0.641568	12808	12862	213-214	1.000000	75.2
atpA-atpF_p5	atpA-atpF	5	CAATGCCATCGCCTACTTGA	GGGGGCGATGAACGAAATAA	20	20	50.0	50.0	58.3	58.3	0.0	0.0	0.661973	12808	12862	200-201	1.000000	75.1
rpoC2_p1	rpoC2	1	CTTGATCCTTGTGGCTCGAA	AGTGATTGCTGAGATTCGCG	20	20	50.0	50.0	57.9	58.7	0.0	0.0	0.814485	20562	20681	233-233	1.000000	73.8
rpoC2_p2	rpoC2	2	TGATCCTTGTGGCTCGAAAG	AGTGATTGCTGAGATTCGCG	20	20	50.0	50.0	57.9	58.7	0.0	0.0	0.814485	20562	20681	231-231	1.000000	73.8
rpoC2_p3	rpoC2	3	CTAGACTGGATCTGCATGGC	AGTGATTGCTGAGATTCGCG	20	20	55.0	50.0	57.8	58.7	0.0	0.0	0.882165	20562	20681	206-206	1.000000	73.3
rpoC2_p4	rpoC2	4	TAGACTGGATCTGCATGGCT	AGTGATTGCTGAGATTCGCG	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.916717	20562	20681	205-205	1.000000	73.1
rpoC2_p5	rpoC2	5	GGCTCGAAAGGGAGACTAGA	AGTGATTGCTGAGATTCGCG	20	20	55.0	50.0	58.2	58.7	0.0	0.0	0.950113	20562	20681	221-221	1.000000	72.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATCCTGGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.367403	28220	29501	1362-1362	1.000000	77.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GTTCTTCCATCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.579026	28220	29501	1441-1441	1.000000	75.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGTTCTTCCATCAAGCCCTG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.586023	28220	29501	1442-1442	1.000000	75.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCCTCATTTCCATCCTGGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.7	58.4	0.0	14.6	0.640766	28220	29501	1344-1344	1.000000	74.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTTCCATCAAGCCCTGATCA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.805450	28220	29501	1438-1438	1.000000	73.6
ndhC_p1	ndhC	1	GGTTGTTCGATCAAGTAAGGGA	CCACGTTCATAGGAGTGTGT	22	20	45.5	50.0	58.1	57.3	9.1	9.3	2.809568	52392	52754	484-484	1.000000	57.5
ndhC_p2	ndhC	2	TGGGTTGTTCGATCAAGTAAGG	CCACGTTCATAGGAGTGTGT	22	20	45.5	50.0	58.3	57.3	9.1	9.3	3.073010	52392	52754	486-486	1.000000	55.4
ndhC_p3	ndhC	3	GGTTGTTCGATCAAGTAAGGGA	TCCACGTTCATAGGAGTGTG	22	20	45.5	50.0	58.1	57.0	9.1	4.6	3.096082	52392	52754	485-485	1.000000	55.2
ndhC_p4	ndhC	4	GGGTTGTTCGATCAAGTAAGG	CCACGTTCATAGGAGTGTGT	21	20	47.6	50.0	56.6	57.3	9.1	9.3	3.119795	52392	52754	485-485	1.000000	55.0
ndhC_p5	ndhC	5	GGTTGTTCGATCAAGTAAGGG	CCACGTTCATAGGAGTGTGT	21	20	47.6	50.0	56.6	57.3	9.1	9.3	3.119795	52392	52754	484-484	1.000000	55.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	GCATGCTGGGTCTTTGAAAC	20	20	50.0	50.0	57.9	57.9	5.2	11.4	0.209585	52755	53836	1277-1277	1.000000	78.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AATGCATGCTGGGTCTTTGA	20	20	50.0	45.0	57.9	58.1	5.2	20.3	0.211836	52755	53836	1280-1280	1.000000	78.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAACTCCGGAAAT	ATGCATGCTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.1	5.2	20.3	0.211836	52755	53836	1279-1279	1.000000	78.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	52755	53836	1213-1213	1.000000	77.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCTTTGCTAATCGGGGCTAA	GCATGCTGGGTCTTTGAAAC	20	20	50.0	50.0	57.7	57.9	0.0	11.4	0.412108	52755	53836	1289-1289	1.000000	76.8
psaI-ycf4_p1	psaI-ycf4	1	ATTTCCGGCAATTGCAATGG	GGATAACGGCCCAGCATAAA	20	20	45.0	50.0	58.0	58.0	30.3	0.0	0.051344	62353	62777	567-568	1.000000	79.7
psaI-ycf4_p2	psaI-ycf4	2	ATTTCCGGCAATTGCAATGG	TTGTTTTTCGAGACCCCGTT	20	20	45.0	45.0	58.0	58.0	30.3	0.0	0.068254	62353	62777	543-544	1.000000	79.6
psaI-ycf4_p3	psaI-ycf4	3	GGCAATTGCAATGGCTTCTT	GGATAACGGCCCAGCATAAA	20	20	45.0	50.0	57.9	58.0	32.4	0.0	0.127877	62353	62777	561-562	1.000000	79.1
psaI-ycf4_p4	psaI-ycf4	4	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCCGTT	20	20	45.0	45.0	57.9	58.0	32.4	0.0	0.144788	62353	62777	537-538	1.000000	79.0
psaI-ycf4_p5	psaI-ycf4	5	GGCCTAGTATTTCCGGCAAT	GGATAACGGCCCAGCATAAA	20	20	50.0	50.0	57.7	58.0	4.4	0.0	0.296284	62353	62777	575-576	1.000000	77.8
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AGTACTTAGTACCGGTGCCA	20	20	50.0	50.0	58.1	57.8	0.0	19.9	0.320407	69977	70420	546-547	1.000000	77.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	AGTACTTAGTACCGGTGCCA	20	20	50.0	50.0	58.1	57.8	0.0	19.9	0.321751	69977	70420	545-546	1.000000	77.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AGTACTTAGTACCGGTGCCA	20	20	50.0	50.0	58.1	57.8	0.0	19.9	0.328443	69977	70420	557-558	1.000000	77.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	AGTACTTAGTACCGGTGCCA	20	20	50.0	50.0	58.1	57.8	0.0	19.9	0.329777	69977	70420	556-557	1.000000	77.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	AGTACTTAGTACCGGTGCCA	20	20	50.0	50.0	58.1	57.8	0.0	19.9	0.329777	69977	70420	559-560	1.000000	77.5
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	GGCCCAATGGAGTTCATAGG	20	20	50.0	55.0	58.0	58.0	7.6	0.0	0.022318	90072	90571	852-879	1.000000	82.3
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GGGCCCAATGGAGTTCATAG	20	20	50.0	55.0	58.0	58.0	7.6	19.9	0.022318	90072	90571	853-880	1.000000	82.3
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	ATTGGCATGCATCCAGTAGG	GGCCCAATGGAGTTCATAGG	20	20	50.0	55.0	58.0	58.0	21.4	0.0	0.021997	90072	90571	901-928	1.000000	82.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	ATTGGCATGCATCCAGTAGG	GGGCCCAATGGAGTTCATAG	20	20	50.0	55.0	58.0	58.0	21.4	19.9	0.021997	90072	90571	902-929	1.000000	82.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	ATGACCTCCTCCTCTATGCC	GGCCCAATGGAGTTCATAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.035835	90072	90571	1527-1554	1.000000	81.1
ycf1_p1	ycf1	1	ACAGGAGGTTTTTCATGACT	TGTAGACATGGGATTTTGTTCT	20	22	40.0	36.4	54.5	55.4	0.0	0.0	8.190485	113106	115430	2479-2509	1.000000	41.1
ycf1_p2	ycf1	2	AACAGGAGGTTTTTCATGACT	TGTAGACATGGGATTTTGTTCT	21	22	38.1	36.4	55.3	55.4	0.0	0.0	8.333673	113106	115430	2480-2510	1.000000	41.1
ycf1_p3	ycf1	3	ACAGGAGGTTTTTCATGACTT	TGTAGACATGGGATTTTGTTCT	21	22	38.1	36.4	55.3	55.4	0.0	0.0	8.333673	113106	115430	2479-2509	1.000000	41.1
ycf1_p4	ycf1	4	ACAGGAGGTTTTTCATGACT	GGGATTTTGTTCTATGATTTGTGC	20	24	40.0	37.5	54.5	57.1	0.0	0.0	8.433877	113106	115430	2470-2500	1.000000	41.1
ycf1_p5	ycf1	5	ACAGGAGGTTTTTCATGACT	TTGTAGACATGGGATTTTGTTCT	20	23	40.0	34.8	54.5	56.1	0.0	0.0	8.446175	113106	115430	2480-2510	1.000000	41.1
ycf1_p1	ycf1	1	CTGAATATTCCGCGACGTGA	GTGTGATGAGAAAACTGAAAG	20	21	50.0	38.1	58.2	53.1	8.2	0.0	6.082269	132434	132553	243-279	1.000000	44.3
ycf1_p2	ycf1	2	TCTGAATATTCCGCGACGTG	GTGTGATGAGAAAACTGAAAG	20	21	50.0	38.1	58.2	53.1	8.2	0.0	6.082269	132434	132553	244-280	1.000000	44.3
ycf1_p3	ycf1	3	AGTTGAAGCCGTGTTTCTGA	GTGTGATGAGAAAACTGAAAG	20	21	45.0	38.1	57.7	53.1	3.1	0.0	6.242973	132434	132553	259-295	1.000000	44.3
ycf1_p4	ycf1	4	TGAATATTCCGCGACGTGAC	GTGTGATGAGAAAACTGAAAG	20	21	50.0	38.1	58.4	53.1	8.2	0.0	6.365164	132434	132553	242-278	1.000000	44.3
ycf1_p5	ycf1	5	TTCTGAATATTCCGCGACGT	GTGTGATGAGAAAACTGAAAG	20	21	45.0	38.1	57.4	53.1	8.2	0.0	6.489580	132434	132553	245-281	1.000000	44.3
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GGCCCAATGGAGTTCATAGG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.0	58.0	0.0	7.6	0.022318	157142	157641	852-879	1.000000	82.3
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	GGGCCCAATGGAGTTCATAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.0	58.0	19.9	7.6	0.022318	157142	157641	853-880	1.000000	82.3
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	GGCCCAATGGAGTTCATAGG	ATTGGCATGCATCCAGTAGG	20	20	55.0	50.0	58.0	58.0	0.0	21.4	0.021997	157142	157641	901-928	1.000000	82.2
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	GGGCCCAATGGAGTTCATAG	ATTGGCATGCATCCAGTAGG	20	20	55.0	50.0	58.0	58.0	19.9	21.4	0.021997	157142	157641	902-929	1.000000	82.2
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	GGCCCAATGGAGTTCATAGG	ATGACCTCCTCCTCTATGCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.035835	157142	157641	1554-1554	0.500000	59.7
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	TTGTCCACCCTTTCCAAGTG	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.331736	158181	159180	1186-1186	0.500000	57.3
rpl2_p2	rpl2	2	CGCTTCAACCGGGTTATTCT	CTTCCCAAACCGTACAAGCT	20	20	50.0	50.0	58.3	58.1	13.0	0.0	0.373628	158181	159180	1088-1088	0.500000	57.0
rpl2_p3	rpl2	3	ATCATTACGCTTCAACCGGG	TTGTCCACCCTTTCCAAGTG	20	20	50.0	50.0	58.3	57.9	6.3	0.0	0.402204	158181	159180	1193-1193	0.500000	56.8
rpl2_p4	rpl2	4	ATCATTACGCTTCAACCGGG	CTTCCCAAACCGTACAAGCT	20	20	50.0	50.0	58.3	58.1	6.3	0.0	0.444096	158181	159180	1095-1095	0.500000	56.4
rpl2_p5	rpl2	5	CGCTTCAACCGGGTTATTCT	TCAAAACCCCTTCCCAAACC	20	20	50.0	50.0	58.3	58.2	13.0	0.0	0.469821	158181	159180	1097-1097	0.500000	56.2