primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	125	426	748-799	1.000000	87.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	125	426	762-813	1.000000	87.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TATTTGTAGGGCGAACGACG	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	58.1	58.0	0.0	10.2	0.130685	125	426	813-813	0.090909	42.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TATTTGTAGGGCGAACGACG	CAGTGCTATGCATGGTTCCT	20	20	50.0	50.0	58.1	58.0	0.0	10.2	0.136443	125	426	812-812	0.090909	42.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TATTTGTAGGGCGAACGACG	GGTGTAGCTGGTGTATTCGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.152066	125	426	844-844	0.090909	42.4
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	CCCCATAGGCTTTCGCTTTC	CTAGTTCCGGGTTCGAATCC	20	20	55.0	55.0	59.0	57.8	0.0	18.0	1.195438	1486	1711	285-308	1.000000	80.0
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TCGCGTCTCTCTAAAATTGCA	CTAGTTCCGGGTTCGAATCC	21	20	42.9	55.0	58.0	57.8	5.8	18.0	1.266163	1486	1711	267-290	1.000000	79.8
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	CCCCATAGGCTTTCGCTTT	CTAGTTCCGGGTTCGAATCC	19	20	52.6	55.0	57.8	57.8	0.0	18.0	1.411661	1486	1711	285-308	1.000000	78.2
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	CGCGTCTCTCTAAAATTGCAG	CTAGTTCCGGGTTCGAATCC	21	20	47.6	55.0	57.7	57.8	0.0	18.0	1.516032	1486	1711	266-289	1.000000	77.8
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	CCCATAGGCTTTCGCTTTCG	CTAGTTCCGGGTTCGAATCC	20	20	55.0	55.0	59.3	57.8	0.0	18.0	1.562639	1486	1711	284-307	1.000000	77.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6162	7250	1113-1201	1.000000	88.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6162	7250	1096-1184	1.000000	87.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6162	7250	1114-1202	1.000000	87.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6162	7250	1111-1199	1.000000	85.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6162	7250	1110-1198	1.000000	85.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8581	9481	824-970	1.000000	62.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8581	9481	823-969	1.000000	62.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8581	9481	827-973	1.000000	61.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8581	9481	826-972	1.000000	61.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8581	9481	825-971	1.000000	61.6
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.0	52.4	0.0	0.0	6.598602	10262	10443	252-279	1.000000	52.0
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	ACCCTTAGCCTTCCAAGCTA	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.0	52.4	0.0	0.0	6.601918	10262	10443	258-285	1.000000	52.0
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.001259	10262	10443	241-268	1.000000	52.0
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.005652	10262	10443	240-267	1.000000	52.0
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	TAACCCTTAGCCTTCCAAGC	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	57.2	52.4	0.0	0.0	7.369840	10262	10443	260-287	1.000000	52.0
atpA-atpF_p1	atpA-atpF	1	CACTTGAAGTACGGTACCGG	ACATGTTGGGAGCGATGAAA	20	20	55.0	45.0	58.0	57.8	35.9	0.0	0.212129	12117	12192	194-259	1.000000	87.8
atpA-atpF_p2	atpA-atpF	2	CCACTTGAAGTACGGTACCG	ACATGTTGGGAGCGATGAAA	20	20	55.0	45.0	58.0	57.8	32.2	0.0	0.212129	12117	12192	195-260	1.000000	87.8
atpA-atpF_p3	atpA-atpF	3	CAATACCATCGCCCACTTGA	ACATGTTGGGAGCGATGAAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.235784	12117	12192	207-272	1.000000	87.3
atpA-atpF_p4	atpA-atpF	4	CACTTGAAGTACGGTACCGG	GACATGTTGGGAGCGATGAA	20	20	55.0	50.0	58.0	58.3	35.9	0.0	0.275464	12117	12192	195-260	1.000000	87.3
atpA-atpF_p5	atpA-atpF	5	CCACTTGAAGTACGGTACCG	GACATGTTGGGAGCGATGAA	20	20	55.0	50.0	58.0	58.3	32.2	0.0	0.275464	12117	12192	196-261	1.000000	87.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	57.0	58.1	10.9	0.0	2.093591	44665	45507	702-953	1.000000	70.9
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATTCGCGATCTAGGC	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	57.0	58.2	10.9	0.0	2.150577	44665	45507	723-974	1.000000	70.4
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCATTTATTCGCGATCTAGGC	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	57.0	57.8	10.9	5.6	2.191892	44665	45507	673-924	1.000000	70.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CCATTTATTCGCGATCTAGGC	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	57.0	58.4	10.9	0.0	2.386552	44665	45507	703-954	1.000000	68.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TCCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	22	20	45.5	50.0	58.4	58.1	10.9	0.0	2.508500	44665	45507	703-954	1.000000	67.6
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.6	57.9	0.0	0.0	0.547734	46510	46900	492-536	1.000000	85.0
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.6	57.8	0.0	0.0	0.655487	46510	46900	460-504	1.000000	84.5
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.840655	46510	46900	491-535	1.000000	82.7
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GATCACTTCCGGCTTTAGGC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.832259	46510	46900	521-565	1.000000	82.5
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GTTAGTCCTGGTAAAGCCCC	CCGCTTAGCTCAGAGGTTAG	20	20	55.0	55.0	57.6	57.5	0.0	7.6	0.915502	46510	46900	500-544	1.000000	82.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46974	47625	698-720	1.000000	84.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46974	47625	730-752	1.000000	83.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46974	47625	692-714	1.000000	83.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	46974	47625	730-752	1.000000	82.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46974	47625	724-746	1.000000	82.5
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	58.0	58.2	10.3	0.0	0.194095	48596	49231	721-819	0.909091	81.9
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CTACATTGGGAATGGTCGGG	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	58.0	58.2	0.0	0.0	0.224271	48596	49231	738-836	0.909091	81.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	TGCTACATTGGGAATGGTCG	ATCATCCACGCTTGAAACGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.228547	48596	49231	740-838	0.909091	81.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.243485	48596	49231	685-783	0.909091	81.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGATGGGATGCTACATTGGG	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	58.1	58.2	0.0	0.0	0.276448	48596	49231	748-846	0.909091	81.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCCCGAAATGAAAAATGCC	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.187644	50902	51944	1047-1235	1.000000	87.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTCTGGCCCCTTTCTAATCG	GCACGTTGGGTCTTTGAAAC	20	20	55.0	50.0	57.8	58.2	0.0	0.0	0.411598	50902	51944	1076-1264	1.000000	85.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCCCGAAATGAAAAATGCC	TGCACGTTGGGTCTTTGAAA	20	20	50.0	45.0	58.0	58.5	0.0	0.0	0.558031	50902	51944	1048-1236	1.000000	84.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGCTAAAACCCCCGAAATGA	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.322827	50902	51944	1055-1243	0.909091	82.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGCTAAAACCCCCGAAATGA	TGCACGTTGGGTCTTTGAAA	20	20	50.0	45.0	58.2	58.5	0.0	0.0	0.693215	50902	51944	1056-1244	0.909091	79.6
atpB-rbcL_p1	atpB-rbcL	1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	54932	55702	848-886	1.000000	83.7
atpB-rbcL_p2	atpB-rbcL	2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.422541	54932	55702	889-927	1.000000	83.3
atpB-rbcL_p3	atpB-rbcL	3	CCGGACCTATGATTTGGACG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.435629	54932	55702	897-935	1.000000	83.2
atpB-rbcL_p4	atpB-rbcL	4	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	54932	55702	919-957	1.000000	82.8
atpB-rbcL_p5	atpB-rbcL	5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.659357	54932	55702	849-887	1.000000	81.6
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	63807	64839	1062-1145	1.000000	84.1
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	63807	64839	1064-1147	1.000000	84.1
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	63807	64839	1061-1144	1.000000	83.6
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	63807	64839	1063-1146	1.000000	83.6
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	63807	64839	1061-1144	1.000000	83.2
ndhF_p1	ndhF	1	CGATGCAACAACAAGACGTT	ACGTCGAATTTTGCAATCCG	20	20	45.0	45.0	57.6	57.8	0.0	0.0	0.624557	109894	112179	2765-2825	0.727273	67.4
ndhF_p2	ndhF	2	TCGATGCAACAACAAGACGT	ACGTCGAATTTTGCAATCCG	20	20	45.0	45.0	58.4	57.8	0.0	0.0	0.663791	109894	112179	2766-2826	0.727273	67.1
ndhF_p3	ndhF	3	CGATGCAACAACAAGACGTT	CGTCGAATTTTGCAATCCGA	20	20	45.0	45.0	57.6	57.5	0.0	0.0	0.902300	109894	112179	2764-2824	0.727273	65.2
ndhF_p4	ndhF	4	TTCGATGCAACAACAAGACG	ACGTCGAATTTTGCAATCCG	20	20	45.0	45.0	57.3	57.8	0.0	0.0	0.902763	109894	112179	2767-2827	0.727273	65.2
ndhF_p5	ndhF	5	TCGATGCAACAACAAGACGT	CGTCGAATTTTGCAATCCGA	20	20	45.0	45.0	58.4	57.5	0.0	0.0	0.941534	109894	112179	2765-2825	0.727273	64.9
ndhF-rpl32_p1	ndhF-rpl32	1	GCAAGAGGTCGTGTAAACCA	ACGATGCACAATACCCCTTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.164689	112180	112972	1182-1398	1.000000	89.5
ndhF-rpl32_p2	ndhF-rpl32	2	AATAGAGCAGCACACAACGT	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.208219	112180	112972	1368-1584	1.000000	88.8
ndhF-rpl32_p3	ndhF-rpl32	3	GCAGCACACAACGTAACAAA	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.219812	112180	112972	1362-1578	1.000000	88.7
ndhF-rpl32_p4	ndhF-rpl32	4	GCAAGAGGTCGTGTAAACCA	TTCTCAAAGCGGCGAGAATT	20	20	50.0	45.0	58.1	58.2	0.0	13.0	0.317892	112180	112972	1395-1610	1.000000	88.3
ndhF-rpl32_p5	ndhF-rpl32	5	AAACCAAAATCCCCTACCCG	ACGATGCACAATACCCCTTC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.263769	112180	112972	1247-1452	0.727273	74.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTTTTGTGCGACAAACAAAT	TTTAGGAGATGACATGCCGC	20	20	30.0	50.0	53.4	58.0	12.1	0.0	4.669997	113135	114036	765-1025	1.000000	52.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	20	20	30.0	50.0	53.4	57.9	12.1	0.0	4.747833	113135	114036	710-970	1.000000	52.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	20	20	30.0	45.0	53.4	57.8	12.1	0.0	4.808560	113135	114036	703-963	1.000000	51.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTTTGTGCGACAAACAAAT	CCTAAGAGCAGCGTGTCTAC	20	20	30.0	55.0	53.4	57.8	12.1	0.0	4.819851	113135	114036	709-969	1.000000	51.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	20	20	30.0	55.0	53.4	57.8	12.1	0.0	4.819851	113135	114036	708-968	1.000000	51.6
ycf1_p1	ycf1	1	CTTCCTTTCCATTGACTCGGA	GCAGAATACCGTCACCCATT	21	20	47.6	50.0	57.7	58.0	0.0	0.0	1.372969	128147	128266	204-233	0.909091	77.4
ycf1_p2	ycf1	2	TCTTCCTTTCCATTGACTCGG	GCAGAATACCGTCACCCATT	21	20	47.6	50.0	57.7	58.0	0.0	0.0	1.372969	128147	128266	205-234	0.909091	77.4
ycf1_p3	ycf1	3	TTCCTTTCCATTGACTCGGA	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	56.5	58.0	0.0	0.0	1.575227	128147	128266	203-232	0.909091	75.8
ycf1_p4	ycf1	4	CTTCCTTTCCATTGACTCGGA	CGGAAGAAAGGGTGGAAAGT	21	20	47.6	50.0	57.7	57.7	0.0	0.0	1.606526	128147	128266	171-182	0.727273	67.1
ycf1_p5	ycf1	5	CTTCCTTTCCATTGACTCGGA	ACGGAAGAAAGGGTGGAAAG	21	20	47.6	50.0	57.7	57.7	0.0	0.0	1.606526	128147	128266	172-183	0.727273	67.0