primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	49362	50007	713-719	1.000000	82.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	49362	50007	701-707	1.000000	82.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	20	20	55.0	50.0	57.9	57.7	0.0	0.0	0.412322	49362	50007	710-716	1.000000	82.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	20	20	50.0	50.0	57.8	57.8	15.5	0.0	0.465562	49362	50007	745-751	1.000000	81.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	49362	50007	733-739	1.000000	81.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.065013	51038	51711	779-806	1.000000	84.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	51038	51711	780-807	1.000000	83.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TCCACGCCTGAAACGTATTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.224747	51038	51711	811-838	1.000000	82.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CATCCACGCCTGAAACGTAT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.399908	51038	51711	813-840	1.000000	81.2
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.448485	51038	51711	812-839	1.000000	80.8
ndhC-atpB_p1	ndhC-atpB	1	AGTACCGGAAACTCAGTGGA	TGAATAGGGGATGTTGTGCG	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.042328	53398	55528	2968-2968	0.500000	59.7
ndhC-atpB_p2	ndhC-atpB	2	AGTACCGGAAACTCAGTGGA	CATCCAGTACCGGACCAATG	20	20	50.0	55.0	58.0	58.0	9.5	9.5	0.051811	53398	55528	2667-2667	0.500000	59.6
ndhC-atpB_p3	ndhC-atpB	3	AGTACCGGAAACTCAGTGGA	ATTGTATAAAGGCAGGCGCA	20	20	50.0	45.0	58.0	57.9	9.5	10.8	0.056705	53398	55528	2994-2994	0.500000	59.5
ndhC-atpB_p4	ndhC-atpB	4	GAAACTCATCGCCCATGGAT	TGAATAGGGGATGTTGTGCG	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.064085	53398	55528	2778-2778	0.500000	59.5
ndhC-atpB_p5	ndhC-atpB	5	GAAACTCATCGCCCATGGAT	CATCCAGTACCGGACCAATG	20	20	50.0	55.0	58.0	58.0	15.2	9.5	0.073569	53398	55528	2477-2477	0.500000	59.4
atpB_p1	atpB	1	TATGGCATTCACCCACGAAG	TATTATTTGGACGCACCGCA	20	20	50.0	45.0	58.0	58.0	4.8	0.0	0.062546	55529	57016	2493-2493	0.500000	59.5
atpB_p2	atpB	2	TATGGCATTCACCCACGAAG	CCGCCCATCAGAGCTATTTT	20	20	50.0	50.0	58.0	58.0	4.8	0.0	0.064085	55529	57016	2257-2257	0.500000	59.5
atpB_p3	atpB	3	ACCAATCAGCTTGCTATCGG	TATTATTTGGACGCACCGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.066547	55529	57016	1984-1984	0.500000	59.5
atpB_p4	atpB	4	ACCAATCAGCTTGCTATCGG	CCGCCCATCAGAGCTATTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068086	55529	57016	1748-1748	0.500000	59.5
atpB_p5	atpB	5	TATGGCATTCACCCACGAAG	TACGATGGGTCCCTTTTCCT	20	20	50.0	50.0	58.0	58.0	4.8	10.0	0.075825	55529	57016	2778-2778	0.500000	59.4
atpE_p1	atpE	1	TTGGTTTGACTGCCCTAACC	TGGGTTCTATTTGGGGCCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.8	0.021754	57013	57414	1803-1803	0.500000	59.8
atpE_p2	atpE	2	GCAGTTTATGTACCTGCGGA	TGGGTTCTATTTGGGGCCTA	20	20	50.0	50.0	58.0	58.0	5.5	0.8	0.030002	57013	57414	1573-1573	0.500000	59.8
atpE_p3	atpE	3	TTGGTTTGACTGCCCTAACC	TATTATTTGGACGCACCGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.035882	57013	57414	1178-1178	0.500000	59.7
atpE_p4	atpE	4	TTGGTTTGACTGCCCTAACC	TGGGCTGGGTATTCACCTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036265	57013	57414	1842-1842	0.500000	59.7
atpE_p5	atpE	5	GCAGTTTATGTACCTGCGGA	TATTATTTGGACGCACCGCA	20	20	50.0	45.0	58.0	58.0	5.5	0.0	0.044130	57013	57414	948-948	0.500000	59.6
trnM-CAU-rbcL_p1	trnM-CAU-rbcL	1	TGCGGTGCGTCCAAATAATA	CGGTCTCTCCAACGCATAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.030171	57688	58807	2035-2035	0.500000	59.8
trnM-CAU-rbcL_p2	trnM-CAU-rbcL	2	TGCGGTGCGTCCAAATAATA	GTAAGTCCATCGGTCCACAC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.031559	57688	58807	1609-1609	0.500000	59.7
trnM-CAU-rbcL_p3	trnM-CAU-rbcL	3	AAAATAGCTCTGATGGGCGG	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031710	57688	58807	2271-2271	0.500000	59.7
trnM-CAU-rbcL_p4	trnM-CAU-rbcL	4	AAAATAGCTCTGATGGGCGG	GTAAGTCCATCGGTCCACAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.033098	57688	58807	1845-1845	0.500000	59.7
trnM-CAU-rbcL_p5	trnM-CAU-rbcL	5	TATTGGGGTTCTGCCGATTG	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.055917	57688	58807	1989-1989	0.500000	59.6
rbcL-psaI_p1	rbcL-psaI	1	TTGTCCCTTTCGTCCTCTCT	CAAACGGGTGATTTTCTCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047375	61953	62102	1312-1312	0.500000	59.6
rbcL-psaI_p2	rbcL-psaI	2	CTGCGGTCTCTGTTGTAGTC	CAAACGGGTGATTTTCTCGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.052281	61953	62102	1231-1231	0.500000	59.6
rbcL-psaI_p3	rbcL-psaI	3	TTGTCCCTTTCGTCCTCTCT	GGATTCCAAGTGTCCGGATC	20	20	50.0	55.0	58.0	58.0	0.0	7.8	0.056195	61953	62102	1089-1089	0.500000	59.6
rbcL-psaI_p4	rbcL-psaI	4	CTGCGGTCTCTGTTGTAGTC	GGATTCCAAGTGTCCGGATC	20	20	55.0	55.0	58.0	58.0	0.0	7.8	0.061101	61953	62102	1008-1008	0.500000	59.5
rbcL-psaI_p5	rbcL-psaI	5	TTGTCCCTTTCGTCCTCTCT	TGATTTTCTCGCCGACTACG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106978	61953	62102	1304-1304	0.500000	59.1
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	GATCGGTTCAGTCGGTGTTT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.362848	67174	68282	1242-1272	1.000000	83.2
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	ATCGGTTCAGTCGGTGTTTT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.404697	67174	68282	1241-1271	1.000000	82.9
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	67174	68282	1207-1237	1.000000	82.1
petA-psbJ_p4	petA-psbJ	4	GATCCATCACGTGTCCAAGG	GATCGGTTCAGTCGGTGTTT	20	20	55.0	50.0	58.3	58.2	16.5	0.0	0.551342	67174	68282	1260-1290	1.000000	81.6
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTGGC	CGGTTCAGTCGGTGTTTTCT	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.576271	67174	68282	1239-1269	1.000000	81.5
rps12-psbB_p1	rps12-psbB	1	TGGCTGGTTTAGATCGATCC	TTTTATCAAGCCCGAGGAGC	20	20	50.0	50.0	57.1	58.0	21.7	0.0	0.949075	74240	74359	438-438	0.500000	52.4
rps12-psbB_p2	rps12-psbB	2	TGGCTGGTTTAGATCGATCC	CAAGTTCAAGACGCGTAGGA	20	20	50.0	50.0	57.1	57.9	21.7	14.0	0.971013	74240	74359	333-333	0.500000	52.2
rps12-psbB_p3	rps12-psbB	3	TGGCTGGTTTAGATCGATCC	TCAAGTTCAAGACGCGTAGG	20	20	50.0	50.0	57.1	57.9	21.7	14.0	0.971013	74240	74359	334-334	0.500000	52.2
rps12-psbB_p4	rps12-psbB	4	TGGCTGGTTTAGATCGATCC	GGTTTTTGAGGAGGGTGAGG	20	20	50.0	55.0	57.1	58.1	21.7	0.0	0.999630	74240	74359	513-513	0.500000	52.0
rps12-psbB_p5	rps12-psbB	5	TGGCTGGTTTAGATCGATCC	GTTTTTGAGGAGGGTGAGGG	20	20	50.0	55.0	57.1	58.1	21.7	0.0	0.999630	74240	74359	512-512	0.500000	52.0
rpl16-rps3_p1	rpl16-rps3	1	GACGAGTCACACACTAAGCA	TTGGATATTTGCGGACGAGG	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.469235	87337	88108	732-818	1.000000	88.2
rpl16-rps3_p2	rpl16-rps3	2	GACGAGTCACACACTAAGCA	GGATATTTGCGGACGAGGAA	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.651639	87337	88108	730-816	1.000000	86.8
rpl16-rps3_p3	rpl16-rps3	3	GACGAGTCACACACTAAGCAT	TTGGATATTTGCGGACGAGG	21	20	47.6	50.0	58.1	58.1	0.0	0.0	1.104043	87337	88108	732-818	1.000000	83.2
rpl16-rps3_p4	rpl16-rps3	4	ACGAGTCACACACTAAGCAT	TTGGATATTTGCGGACGAGG	20	20	45.0	50.0	56.9	58.1	0.0	0.0	1.161110	87337	88108	731-817	1.000000	82.7
rpl16-rps3_p5	rpl16-rps3	5	GACGAGTCACACACTAAGCAT	GGATATTTGCGGACGAGGAA	21	20	47.6	50.0	58.1	57.8	0.0	0.0	1.286447	87337	88108	730-816	1.000000	81.7
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AACTCTTCCTCAGTCAGGCT	ATTTCCCCATACCCCCATGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088500	114681	115250	1649-1649	0.500000	59.3
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AACTCTTCCTCAGTCAGGCT	TTCCCCATACCCCCATGAAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088500	114681	115250	1647-1647	0.500000	59.3
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	ACTCTTCCTCAGTCAGGCTT	ATTTCCCCATACCCCCATGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088500	114681	115250	1648-1648	0.500000	59.3
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	ACTCTTCCTCAGTCAGGCTT	TTCCCCATACCCCCATGAAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088500	114681	115250	1646-1646	0.500000	59.3
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	ACTCTTCCTCAGTCAGGCTT	AGTCTTGGTTCGACTCATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.108772	114681	115250	1217-1217	0.500000	59.1
ccsA-ndhD_p1	ccsA-ndhD	1	TTCCGCAATTGTGGCTTCTA	TCCGGATTTCGTTCTCTCAC	20	20	45.0	50.0	57.8	57.4	29.6	0.0	0.841995	120984	121187	389-436	1.000000	76.8
ccsA-ndhD_p2	ccsA-ndhD	2	TTCCGCAATTGTGGCTTCTA	CCGGATTTCGTTCTCTCACT	20	20	45.0	50.0	57.8	57.3	29.6	0.0	0.849224	120984	121187	388-435	1.000000	76.7
ccsA-ndhD_p3	ccsA-ndhD	3	AATTCCGCAATTGTGGCTTC	TCCGGATTTCGTTCTCTCAC	20	20	45.0	50.0	57.6	57.4	29.6	0.0	1.017986	120984	121187	391-438	1.000000	75.3
ccsA-ndhD_p4	ccsA-ndhD	4	AATTCCGCAATTGTGGCTTC	CCGGATTTCGTTCTCTCACT	20	20	45.0	50.0	57.6	57.3	29.6	0.0	1.025215	120984	121187	390-437	1.000000	75.3
ccsA-ndhD_p5	ccsA-ndhD	5	TCCGCAATTGTGGCTTCTAT	TCCGGATTTCGTTCTCTCAC	20	20	45.0	50.0	57.6	57.4	29.6	0.0	1.067699	120984	121187	388-435	1.000000	75.0
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TGACTCTTATGGGCCAGTGA	GACCTGGACCGAGATGGATA	20	20	50.0	55.0	58.0	58.0	13.8	0.0	0.051992	130107	130226	798-798	0.500000	59.6
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	GCGTGTGTACATCTGTTTGC	GACCTGGACCGAGATGGATA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052730	130107	130226	1212-1212	0.500000	59.6
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	AAATTGGCCGCAGTTCCTTA	GACCTGGACCGAGATGGATA	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.085330	130107	130226	985-985	0.500000	59.3
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	TAAATTGGCCGCAGTTCCTT	GACCTGGACCGAGATGGATA	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.085330	130107	130226	986-986	0.500000	59.3
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	TCTGTAAATTGGCCGCAGTT	GACCTGGACCGAGATGGATA	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.098915	130107	130226	990-990	0.500000	59.2
rpl14_p1	rpl14	1	TTGCATTGAAAGAAACGATT	ATTGTATCGTGGTCTCGTCT	20	20	30.0	45.0	52.2	56.1	0.0	0.0	7.659199	163963	164331	427-427	0.500000	20.0
rpl14_p2	rpl14	2	TTTGCATTGAAAGAAACGAT	ATTGTATCGTGGTCTCGTCT	20	20	30.0	45.0	52.2	56.1	0.0	0.0	7.659199	163963	164331	428-428	0.500000	20.0
rpl14_p3	rpl14	3	TTTGCATTGAAAGAAACGATT	ATTGTATCGTGGTCTCGTCT	21	20	28.6	45.0	53.2	56.1	0.0	0.0	7.720104	163963	164331	428-428	0.500000	20.0
rpl14_p4	rpl14	4	TTGCATTGAAAGAAACGATT	TTGTATCGTGGTCTCGTCTA	20	20	30.0	45.0	52.2	55.5	0.0	0.0	8.295205	163963	164331	426-426	0.500000	20.0
rpl14_p5	rpl14	5	TTTGCATTGAAAGAAACGAT	TTGTATCGTGGTCTCGTCTA	20	20	30.0	45.0	52.2	55.5	0.0	0.0	8.295205	163963	164331	427-427	0.500000	20.0