primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	psbA-matK	1	AAGACGGTTTTCGGTGCTAG	CCGTATCAATGACCTGGTGA	20	20	50.0	50.0	58.2	57.0	0.0	2.8	1.187808	1151	1666	589-662	1.000000	74.6
psbA-matK_p2	psbA-matK	2	AAGACGGTTTTCGGTGCTAG	TCCGTATCAATGACCTGGTG	20	20	50.0	50.0	58.2	57.0	0.0	2.8	1.187808	1151	1666	590-663	1.000000	74.6
psbA-matK_p3	psbA-matK	3	AAAGACGGTTTTCGGTGCTA	CCGTATCAATGACCTGGTGA	20	20	45.0	50.0	57.8	57.0	0.0	2.8	1.223416	1151	1666	590-663	1.000000	74.3
psbA-matK_p4	psbA-matK	4	AAAGACGGTTTTCGGTGCTA	TCCGTATCAATGACCTGGTG	20	20	45.0	50.0	57.8	57.0	0.0	2.8	1.223416	1151	1666	591-664	1.000000	74.3
psbA-matK_p5	psbA-matK	5	TAAAGACGGTTTTCGGTGCT	CCGTATCAATGACCTGGTGA	20	20	45.0	50.0	57.8	57.0	0.0	2.8	1.223416	1151	1666	591-664	1.000000	74.3
matK-rps16_p1	matK-rps16	1	ACGAAGTAGTGTTGACGAGA	GGTGCTCAACCTACAAGAACA	20	21	45.0	47.6	56.3	58.2	0.0	0.0	2.876223	3215	4495	1418-1435	1.000000	57.8
matK-rps16_p2	matK-rps16	2	TGAAAGAGAAGTGGGTAGACG	GGTGCTCAACCTACAAGAACA	21	21	47.6	47.6	57.1	58.2	0.0	0.0	3.090179	3215	4495	1436-1453	1.000000	56.1
matK-rps16_p3	matK-rps16	3	AGAGAAGTGGGTAGACGAAG	GGTGCTCAACCTACAAGAACA	20	21	50.0	47.6	56.0	58.2	0.0	0.0	3.142299	3215	4495	1432-1449	1.000000	55.7
matK-rps16_p4	matK-rps16	4	TTCCTGAAAGAGAAGTGGGT	GGTGCTCAACCTACAAGAACA	20	21	45.0	47.6	56.0	58.2	0.0	0.0	3.177455	3215	4495	1440-1457	1.000000	55.4
matK-rps16_p5	matK-rps16	5	TCCTGAAAGAGAAGTGGGTAGA	GGTGCTCAACCTACAAGAACA	22	21	45.5	47.6	57.9	58.2	0.0	0.0	3.281725	3215	4495	1439-1456	1.000000	54.6
rps16-trnQ-TTG_p1	rps16-trnQ-TTG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5587	6676	1136-1163	1.000000	85.8
rps16-trnQ-TTG_p2	rps16-trnQ-TTG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5587	6676	1147-1174	1.000000	85.3
rps16-trnQ-TTG_p3	rps16-trnQ-TTG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5587	6676	1140-1167	1.000000	84.7
rps16-trnQ-TTG_p4	rps16-trnQ-TTG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5587	6676	1151-1178	1.000000	84.1
rps16-trnQ-TTG_p5	rps16-trnQ-TTG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5587	6676	1142-1169	1.000000	83.0
trnG-GCC-trnfM-CAT_p1	trnG-GCC-trnfM-CAT	1	AATATAGGCCGTAGCAAGCG	CGAGGCTCATAACCTTGAGG	20	20	50.0	55.0	57.9	57.8	0.0	16.0	0.311501	12255	12577	473-543	1.000000	78.9
trnG-GCC-trnfM-CAT_p2	trnG-GCC-trnfM-CAT	2	AATATAGGCCGTAGCAAGCG	CAGTTTTCGGCCCTACAGAG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.353433	12255	12577	518-588	1.000000	78.5
trnG-GCC-trnfM-CAT_p3	trnG-GCC-trnfM-CAT	3	AATATAGGCCGTAGCAAGCG	CAGTTTGGTAGCTCACGAGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.365492	12255	12577	488-558	1.000000	78.4
trnG-GCC-trnfM-CAT_p4	trnG-GCC-trnfM-CAT	4	AATATAGGCCGTAGCAAGCG	CTACAGAGCGGAGTAGAGCA	20	20	50.0	55.0	57.9	58.3	0.0	6.6	0.410682	12255	12577	506-576	1.000000	78.0
trnG-GCC-trnfM-CAT_p5	trnG-GCC-trnfM-CAT	5	AATATAGGCCGTAGCAAGCG	TACAGAGCGGAGTAGAGCAG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.410682	12255	12577	505-575	1.000000	78.0
trnfM-CAT-trnT-GGT_p1	trnfM-CAT-trnT-GGT	1	CCTCAAGGTTATGAGCCTCG	ACCACTAAGTGAAAAGCCCT	20	20	55.0	45.0	57.8	56.7	0.0	0.0	1.579584	12652	14207	1689-1749	1.000000	70.5
trnfM-CAT-trnT-GGT_p2	trnfM-CAT-trnT-GGT	2	CCTCAAGGTTATGAGCCTCG	TGAACCGATGACTTATGCCT	20	20	55.0	45.0	57.8	56.6	0.0	0.0	1.602911	12652	14207	1659-1719	1.000000	70.4
trnfM-CAT-trnT-GGT_p3	trnfM-CAT-trnT-GGT	3	CCTCGTGAGCTACCAAACTG	ACCACTAAGTGAAAAGCCCT	20	20	55.0	45.0	58.3	56.7	0.0	0.0	1.633575	12652	14207	1674-1734	1.000000	70.1
trnfM-CAT-trnT-GGT_p4	trnfM-CAT-trnT-GGT	4	CCTCGTGAGCTACCAAACTG	TGAACCGATGACTTATGCCT	20	20	55.0	45.0	58.3	56.6	0.0	0.0	1.656902	12652	14207	1644-1704	1.000000	70.0
trnfM-CAT-trnT-GGT_p5	trnfM-CAT-trnT-GGT	5	CCTCAAGGTTATGAGCCTCG	CCGATGACTTATGCCTTACCA	20	21	55.0	47.6	57.8	57.5	0.0	0.0	1.700223	12652	14207	1655-1715	1.000000	69.6
trnD-GTC-psbM_p1	trnD-GTC-psbM	1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	20	23	50.0	43.5	57.7	57.9	37.2	0.0	3.378932	15379	16102	882-1215	1.000000	53.3
trnD-GTC-psbM_p2	trnD-GTC-psbM	2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	20	23	50.0	43.5	58.5	57.9	5.4	0.0	3.563435	15379	16102	848-1181	1.000000	51.9
trnD-GTC-psbM_p3	trnD-GTC-psbM	3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	20	23	50.0	43.5	57.7	57.7	37.2	0.0	3.639009	15379	16102	883-1216	1.000000	51.3
trnD-GTC-psbM_p4	trnD-GTC-psbM	4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	20	21	50.0	42.9	57.7	55.5	37.2	0.0	3.794602	15379	16102	883-1216	1.000000	50.0
trnD-GTC-psbM_p5	trnD-GTC-psbM	5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	20	20	50.0	40.0	57.7	54.5	37.2	0.0	3.812375	15379	16102	843-1176	1.000000	49.9
psaA-ycf3_p1	psaA-ycf3	1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	20	20	55.0	45.0	58.0	57.7	24.4	19.9	0.292474	40717	41308	811-817	1.000000	81.1
psaA-ycf3_p2	psaA-ycf3	2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	58.1	57.7	0.0	19.9	0.351778	40717	41308	776-782	1.000000	80.8
psaA-ycf3_p3	psaA-ycf3	3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	20	20	55.0	55.0	58.0	57.6	24.4	18.7	0.373057	40717	41308	778-784	1.000000	80.6
psaA-ycf3_p4	psaA-ycf3	4	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	20	20	50.0	55.0	58.1	57.6	0.0	18.7	0.432361	40717	41308	743-749	1.000000	80.3
psaA-ycf3_p5	psaA-ycf3	5	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.8	57.7	18.1	19.9	0.489521	40717	41308	714-720	1.000000	80.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	46774	47340	677-700	1.000000	79.9
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.213630	46774	47340	730-753	1.000000	79.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	46774	47340	667-690	1.000000	79.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.287539	46774	47340	720-743	1.000000	79.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.335347	46774	47340	728-751	1.000000	78.6
ndhK_p1	ndhK	1	TCTGCACAAAAGAACCCCTC	TCTCTACCCTTGGGCAATGA	20	20	50.0	50.0	58.0	58.0	0.0	5.5	0.053329	47919	48668	890-902	1.000000	80.1
ndhK_p2	ndhK	2	TCTGCACAAAAGAACCCCTC	CGACGTATTGGGTGTATCCG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.256324	47919	48668	866-878	1.000000	78.5
ndhK_p3	ndhK	3	GATAACCAACCCTGCTGCAT	TCTCTACCCTTGGGCAATGA	20	20	50.0	50.0	58.2	58.0	1.3	5.5	0.266827	47919	48668	988-1000	1.000000	78.3
ndhK_p4	ndhK	4	ATAACCAACCCTGCTGCATT	TCTCTACCCTTGGGCAATGA	20	20	45.0	50.0	57.8	58.0	1.3	5.5	0.271075	47919	48668	987-999	1.000000	78.3
ndhK_p5	ndhK	5	TCTGCACAAAAGAACCCCTC	GGGCAATGAGTTTCGACGTA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.301550	47919	48668	879-891	1.000000	78.1
petL-petG_p1	petL-petG	1	CGGTTTTCTACTGGCTGCTT	ACGCAGTCACGAATAATCCC	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540310	62332	62506	316-324	1.000000	78.2
petL-petG_p2	petL-petG	2	TTCGGTTTTCTACTGGCTGC	ACGCAGTCACGAATAATCCC	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.544659	62332	62506	318-326	1.000000	78.1
petL-petG_p3	petL-petG	3	CGGTTTTCTACTGGCTGCTT	CAGTCACGAATAATCCCGCT	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.701311	62332	62506	313-321	1.000000	76.9
petL-petG_p4	petL-petG	4	TTCGGTTTTCTACTGGCTGC	CAGTCACGAATAATCCCGCT	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.705660	62332	62506	315-323	1.000000	76.9
petL-petG_p5	petL-petG	5	CGGTTTTCTACTGGCTGCTT	CGCCTGTATTGCAAATACGC	20	20	50.0	50.0	58.5	58.3	0.0	1.7	0.734250	62332	62506	332-340	1.000000	76.5
petG-trnW-CCA_p1	petG-trnW-CCA	1	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.1	57.9	0.0	1.4	0.153017	62621	62743	216-220	1.000000	83.6
petG-trnW-CCA_p2	petG-trnW-CCA	2	GCGTATTTGCAATACAGGCG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.3	57.9	0.0	1.4	0.346957	62621	62743	200-204	1.000000	82.4
petG-trnW-CCA_p3	petG-trnW-CCA	3	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.314018	62621	62743	219-223	1.000000	82.2
petG-trnW-CCA_p4	petG-trnW-CCA	4	GGGATTATTCGTGACTGCGT	CCCGATGTCGTAGGTTCAAA	20	20	50.0	50.0	58.1	57.6	0.0	1.4	0.437608	62621	62743	211-215	1.000000	81.4
petG-trnW-CCA_p5	petG-trnW-CCA	5	GGGATTATTCGTGACTGCGT	TCCAAAACCCGATGTCGTAG	20	20	50.0	50.0	58.1	57.6	0.0	1.4	0.437608	62621	62743	218-222	1.000000	81.2
trnP-TGG-psaJ_p1	trnP-TGG-psaJ	1	TCCCCACTACTGCCCATAAA	AAACCTGCTAAAGCCCCAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.121576	63028	63412	562-613	1.000000	81.6
trnP-TGG-psaJ_p2	trnP-TGG-psaJ	2	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.9	57.9	5.7	0.0	0.209515	63028	63412	485-536	1.000000	81.3
trnP-TGG-psaJ_p3	trnP-TGG-psaJ	3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	58.2	57.9	5.7	0.0	0.266772	63028	63412	486-537	1.000000	80.9
trnP-TGG-psaJ_p4	trnP-TGG-psaJ	4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.8	57.9	3.5	0.0	0.285929	63028	63412	493-544	1.000000	80.7
trnP-TGG-psaJ_p5	trnP-TGG-psaJ	5	CCCCACTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	20	20	55.0	45.0	57.7	57.9	0.0	0.0	0.444015	63028	63412	561-612	1.000000	79.1
ndhF_p1	ndhF	1	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	20	21	45.0	42.9	58.2	58.1	0.0	0.0	1.236072	101753	103969	2347-2347	0.333333	43.4
ndhF_p2	ndhF	2	GTTTGCTGGCTTATTTGGCA	AGGTTTGGTGAATCGGAAACA	20	21	45.0	42.9	57.5	58.1	0.0	0.0	1.515145	101753	103969	2486-2486	0.333333	41.2
ndhF_p3	ndhF	3	ACGGAGACTTGCTTCACATT	AGGTTTGGTGAATCGGAAACA	20	21	45.0	42.9	57.4	58.1	0.0	0.0	1.611992	101753	103969	2572-2572	0.333333	40.4
ndhF_p4	ndhF	4	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACA	20	20	45.0	45.0	58.2	56.5	0.0	0.0	1.630618	101753	103969	2346-2346	0.333333	40.3
ndhF_p5	ndhF	5	CGTTTGCTGGCTTATTTGGC	AGGTTTGGTGAATCGGAAACA	20	21	50.0	42.9	58.7	58.1	0.0	0.0	1.711928	101753	103969	2487-2487	0.333333	39.6
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	103970	104798	914-941	1.000000	42.2
ndhF-rpl32_p2	ndhF-rpl32	2	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	103970	104798	915-942	1.000000	42.2
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	103970	104798	916-943	1.000000	42.2
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	103970	104798	917-944	1.000000	42.2
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGGGAGAAGAGGA	TTTTTGACATCGAAGTACGT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.822578	103970	104798	918-945	1.000000	42.2
rpl32-trnL-TAG_p1	rpl32-trnL-TAG	1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.421775	104979	105515	633-636	1.000000	81.7
rpl32-trnL-TAG_p2	rpl32-trnL-TAG	2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.476741	104979	105515	636-639	1.000000	81.2
rpl32-trnL-TAG_p3	rpl32-trnL-TAG	3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.542343	104979	105515	606-609	1.000000	80.9
rpl32-trnL-TAG_p4	rpl32-trnL-TAG	4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.4	57.8	0.0	0.0	0.603070	104979	105515	599-602	1.000000	80.5
rpl32-trnL-TAG_p5	rpl32-trnL-TAG	5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	20	20	50.0	50.0	58.4	58.2	0.0	4.7	0.604553	104979	105515	620-623	1.000000	80.3
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	TCCGTTGATAGGGTAGAGGT	20	20	50.0	50.0	58.0	56.9	0.0	0.0	1.142608	105673	106641	1151-1284	1.000000	71.6
ccsA_p2	ccsA	2	AAGCATCTCGGTTCGAATCC	TCCGTTGATAGGGTAGAGGT	20	20	50.0	50.0	58.1	56.9	0.0	0.0	1.197575	105673	106641	1148-1281	1.000000	71.2
ccsA_p3	ccsA	3	CTCAAGCATCTCGGTTCGAA	TCCGTTGATAGGGTAGAGGTT	20	21	50.0	47.6	58.0	57.6	0.0	0.0	1.397134	105673	106641	1151-1284	1.000000	69.6
ccsA_p4	ccsA	4	AAGCATCTCGGTTCGAATCC	TCCGTTGATAGGGTAGAGGTT	20	21	50.0	47.6	58.1	57.6	0.0	0.0	1.452101	105673	106641	1148-1281	1.000000	69.2
ccsA_p5	ccsA	5	CTCAAGCATCTCGGTTCGAA	ATCCGTTGATAGGGTAGAGGT	20	21	50.0	47.6	58.0	57.4	0.0	0.0	1.618276	105673	106641	1152-1285	1.000000	67.8
psaC-ndhE_p1	psaC-ndhE	1	TACATCTGTTGGGCAAGCTC	TGTTATAGCCCTTGCAGCTG	20	20	50.0	50.0	57.9	58.0	0.0	10.1	0.156546	108533	108999	644-654	1.000000	81.2
psaC-ndhE_p2	psaC-ndhE	2	GGCAAGCTCGTACACATTGA	TGTTATAGCCCTTGCAGCTG	20	20	50.0	50.0	58.6	58.0	0.0	10.1	0.604035	108533	108999	633-643	1.000000	77.7
psaC-ndhE_p3	psaC-ndhE	3	GCAAGCTCGTACACATTGAG	TGTTATAGCCCTTGCAGCTG	20	20	50.0	50.0	57.2	58.0	0.0	10.1	0.837807	108533	108999	632-642	1.000000	75.8
psaC-ndhE_p4	psaC-ndhE	4	ATACATCTGTTGGGCAAGCT	TGTTATAGCCCTTGCAGCTG	20	20	45.0	50.0	57.2	58.0	0.0	10.1	0.857491	108533	108999	645-655	1.000000	75.6
psaC-ndhE_p5	psaC-ndhE	5	TACATCTGTTGGGCAAGCTC	TTGTTATAGCCCTTGCAGCT	20	20	50.0	45.0	57.9	57.2	0.0	4.2	0.920805	108533	108999	645-655	1.000000	75.1