primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoBb-rpoBa_p1	rpoBb-rpoBa	1	AGAGCTGACTTTCTGGGACT	ACAGGTTTTGAAGCACGAGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061105	33069	33758	1402-1418	1.000000	82.9
rpoBb-rpoBa_p2	rpoBb-rpoBa	2	AGAGCTGACTTTCTGGGACT	TGATTCAGGGCATGCTGTTT	20	20	50.0	45.0	58.0	58.1	0.0	18.9	0.095230	33069	33758	1376-1392	1.000000	82.6
rpoBb-rpoBa_p3	rpoBb-rpoBa	3	TGCTCTGCAGAAAGGAAGTG	CACAAACGACGCTTGACTTC	20	20	50.0	50.0	58.1	58.0	18.9	17.3	0.116974	33069	33758	1417-1433	1.000000	82.5
rpoBb-rpoBa_p4	rpoBb-rpoBa	4	AGAGCTGACTTTCTGGGACT	CACAAACGACGCTTGACTTC	20	20	50.0	50.0	58.0	58.0	0.0	17.3	0.027906	33069	33758	1936-1952	1.000000	82.4
rpoBb-rpoBa_p5	rpoBb-rpoBa	5	CTTTCACAGAAGTGCCTCGT	CACAAACGACGCTTGACTTC	20	20	50.0	50.0	58.1	58.0	0.0	17.3	0.134108	33069	33758	1984-2000	1.000000	81.5
psbD-psbA_p1	psbD-psbA	1	CCAGTCATCAGCGTCATCAA	AACGCTCACAACTTCCCATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.033079	44470	45308	874-965	1.000000	91.0
psbD-psbA_p2	psbD-psbA	2	AACCAGTCATCAGCGTCATC	AACGCTCACAACTTCCCATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034479	44470	45308	876-967	1.000000	91.0
psbD-psbA_p3	psbD-psbA	3	CCAGTCATCAGCGTCATCAA	GCGTCTGTAGAAGCTCCTTC	20	20	50.0	55.0	58.0	58.1	0.0	11.7	0.098383	44470	45308	847-938	1.000000	90.7
psbD-psbA_p4	psbD-psbA	4	CCAGTCATCAGCGTCATCAA	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	58.0	58.1	0.0	2.4	0.097063	44470	45308	893-984	1.000000	90.4
psbD-psbA_p5	psbD-psbA	5	AACCAGTCATCAGCGTCATC	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	58.0	58.1	0.0	2.4	0.098463	44470	45308	895-986	1.000000	90.4
psbA_p1	psbA	1	ATGTCAAGGCTGTTCTTTGC	TGATTGGTTTGAACGCAAAA	20	20	45.0	35.0	56.9	54.9	0.0	0.0	4.187112	46092	46211	176-176	0.500000	26.5
psbA_p2	psbA	2	ATGTCAAGGCTGTTCTTTGC	TTGATTGGTTTGAACGCAAA	20	20	45.0	35.0	56.9	54.9	0.0	0.0	4.187112	46092	46211	177-177	0.500000	26.5
psbA_p3	psbA	3	ATGTCAAGGCTGTTCTTTGC	TTTGATTGGTTTGAACGCAA	20	20	45.0	35.0	56.9	54.9	0.0	0.0	4.187112	46092	46211	178-178	0.500000	26.5
psbA_p4	psbA	4	ATGTCAAGGCTGTTCTTTGC	TTTTGATTGGTTTGAACGCA	20	20	45.0	35.0	56.9	54.9	0.0	0.0	4.187112	46092	46211	179-179	0.500000	26.5
psbA_p5	psbA	5	ATGTCAAGGCTGTTCTTTGC	TGATTGGTTTGAACGCAAAAA	20	21	45.0	33.3	56.9	55.7	0.0	0.0	4.374610	46092	46211	176-176	0.500000	25.0
ODF51_pgt004-atpE_p1	ODF51_pgt004-atpE	1	GTAAAGCCACGCTGGTTTTC	GCACGCTTTCAAGTTGTGAA	20	20	50.0	45.0	58.0	57.8	17.3	14.5	0.233634	53900	54076	263-274	1.000000	83.5
ODF51_pgt004-atpE_p2	ODF51_pgt004-atpE	2	GAAAGGGAGGGATTCGAACC	GCACGCTTTCAAGTTGTGAA	20	20	55.0	45.0	58.0	57.8	0.0	14.5	0.227169	53900	54076	293-304	1.000000	83.0
ODF51_pgt004-atpE_p3	ODF51_pgt004-atpE	3	GGAAAGGGAGGGATTCGAAC	GCACGCTTTCAAGTTGTGAA	20	20	55.0	45.0	58.0	57.8	0.0	14.5	0.227169	53900	54076	294-305	1.000000	83.0
ODF51_pgt004-atpE_p4	ODF51_pgt004-atpE	4	GAAAGGGAGGGATTCGAACC	AAGCACGCTTTCAAGTTGTG	20	20	55.0	45.0	58.0	57.8	0.0	2.1	0.233124	53900	54076	295-306	1.000000	82.9
ODF51_pgt004-atpE_p5	ODF51_pgt004-atpE	5	GGAAAGGGAGGGATTCGAAC	AAGCACGCTTTCAAGTTGTG	20	20	55.0	45.0	58.0	57.8	0.0	2.1	0.233124	53900	54076	296-307	1.000000	82.9
chlB-ODF51_pgt007_p1	chlB-ODF51_pgt007	1	CTGGACCAGCATACATCCAA	CAGGATTTGAACCTGCGACT	20	20	50.0	50.0	57.3	58.2	9.0	0.0	0.902236	60104	61955	1951-2067	1.000000	81.5
chlB-ODF51_pgt007_p2	chlB-ODF51_pgt007	2	CTGGACCAGCATACATCCAA	ATTATGAGCCCCACGAGCTA	20	20	50.0	50.0	57.3	58.3	9.0	0.0	0.998718	60104	61955	1926-2042	1.000000	80.8
chlB-ODF51_pgt007_p3	chlB-ODF51_pgt007	3	CTGGACCAGCATACATCCAA	GAACCTGCGACTTTGGGATT	20	20	50.0	50.0	57.3	58.5	9.0	0.0	1.181021	60104	61955	1943-2059	1.000000	79.3
chlB-ODF51_pgt007_p4	chlB-ODF51_pgt007	4	CTGGACCAGCATACATCCAA	CAGGAGCAGGATTTGAACCT	20	20	50.0	50.0	57.3	57.5	9.0	0.0	1.219644	60104	61955	1957-2073	1.000000	78.9
chlB-ODF51_pgt007_p5	chlB-ODF51_pgt007	5	CTGGACCAGCATACATCCAA	AGGATTTGAACCTGCGACTT	20	20	50.0	45.0	57.3	57.4	9.0	0.0	1.284372	60104	61955	1950-2066	1.000000	78.5
infA-psbB_p1	infA-psbB	1	TGGTGACAAAGTGACTGTTGA	AAGTGTCATTGAACCTGCCC	21	20	42.9	50.0	57.7	58.4	12.2	0.0	1.721554	66720	67141	557-612	1.000000	72.0
infA-psbB_p2	infA-psbB	2	TGGTGACAAAGTGACTGTTGA	TGCACAGAAATTAGTCGCCC	21	20	42.9	50.0	57.7	58.6	12.2	0.0	1.893392	66720	67141	508-563	1.000000	70.8
infA-psbB_p3	infA-psbB	3	TGGTGACAAAGTGACTGTTGA	GCCCCGGGTCATTAATAACT	21	20	42.9	50.0	57.7	57.3	12.2	21.8	1.993758	66720	67141	492-547	1.000000	70.1
infA-psbB_p4	infA-psbB	4	TGGTGACAAAGTGACTGTTGA	CCCAACCAGCAACTAAAGCA	21	20	42.9	50.0	57.7	58.7	12.2	0.0	2.015074	66720	67141	540-595	1.000000	69.7
infA-psbB_p5	infA-psbB	5	TGGTGACAAAGTGACTGTTGA	TGCCCAACCAGCAACTAAAG	21	20	42.9	50.0	57.7	58.7	12.2	0.0	2.015074	66720	67141	542-597	1.000000	69.7
psbH-psbK_p1	psbH-psbK	1	ATGGGTGTTTTCATTGGGCT	AAGGTGCATAAGCTTCTGGT	20	20	45.0	45.0	58.0	57.1	0.0	13.2	0.915713	70111	70819	873-875	1.000000	76.9
psbH-psbK_p2	psbH-psbK	2	TGGGTGTTTTCATTGGGCTT	AAGGTGCATAAGCTTCTGGT	20	20	45.0	45.0	58.2	57.1	0.0	13.2	1.087218	70111	70819	872-874	1.000000	75.5
psbH-psbK_p3	psbH-psbK	3	ATGGGTGTTTTCATTGGGCT	AAAGGTGCATAAGCTTCTGGT	20	21	45.0	42.9	58.0	57.8	0.0	13.2	1.189241	70111	70819	874-876	1.000000	74.7
psbH-psbK_p4	psbH-psbK	4	TGGGTGTTTTCATTGGGCTT	AAAGGTGCATAAGCTTCTGGT	20	21	45.0	42.9	58.2	57.8	0.0	13.2	1.360746	70111	70819	873-875	1.000000	73.3
psbH-psbK_p5	psbH-psbK	5	ATGGGTGTTTTCATTGGGCT	ACGATAGGATCAAAAGGTGCA	20	21	45.0	42.9	58.0	57.4	0.0	0.0	1.655956	70111	70819	886-888	1.000000	70.9
ODF51_pgt015-rps4_p1	ODF51_pgt015-rps4	1	GGAGAAAACGGGGATTCGAA	GCACCTTTTCCTCGAAATGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.120925	93513	93818	409-447	1.000000	85.6
ODF51_pgt015-rps4_p2	ODF51_pgt015-rps4	2	TTTGAATGTTTCTGCCCCCA	GCACCTTTTCCTCGAAATGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.111488	93513	93818	461-499	1.000000	85.4
ODF51_pgt015-rps4_p3	ODF51_pgt015-rps4	3	TCCCTTTCACGGAGAAAACG	GCACCTTTTCCTCGAAATGC	20	20	50.0	50.0	58.1	58.0	6.7	0.0	0.143690	93513	93818	419-457	1.000000	85.4
ODF51_pgt015-rps4_p4	ODF51_pgt015-rps4	4	TGAATGTTTCTGCCCCCATC	GCACCTTTTCCTCGAAATGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.166084	93513	93818	459-497	1.000000	85.0
ODF51_pgt015-rps4_p5	ODF51_pgt015-rps4	5	TTGAATGTTTCTGCCCCCAT	GCACCTTTTCCTCGAAATGC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.341386	93513	93818	460-498	1.000000	83.6
atpB_p1	atpB	1	ACCTTTTCGAATACTCGCCC	TACCCTTGGACCACCCTTAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.058815	104750	104869	1538-1538	1.000000	87.5
atpB_p2	atpB	2	CGAACTGTACGTGCACCTTA	TACCCTTGGACCACCCTTAG	20	20	50.0	55.0	57.9	58.0	12.1	0.0	0.093554	104750	104869	1517-1517	1.000000	87.3
atpB_p3	atpB	3	TTCAGGCATGGCTGGATTTT	TACCCTTGGACCACCCTTAG	20	20	45.0	55.0	58.1	58.0	39.4	0.0	0.097490	104750	104869	1268-1268	0.500000	59.2
atpB_p4	atpB	4	ACCTTTTCGAATACTCGCCC	TCCTTGTTGCAGGGAATGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110248	104750	104869	972-972	0.500000	59.1
atpB_p5	atpB	5	ACCTTTTCGAATACTCGCCC	TGTGCAGTTTGTGGTTCAGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.141239	104750	104869	1085-1085	0.500000	58.9
ODF51_pgt021-psbM_p1	ODF51_pgt021-psbM	1	TTTGAGCAAGGAGGGATTCG	ACTGCATCAAATGAATCTGCT	20	21	50.0	38.1	57.9	56.2	0.0	8.5	2.910668	111056	111414	453-459	1.000000	60.4
ODF51_pgt021-psbM_p2	ODF51_pgt021-psbM	2	CCACATGCTCTAGTCCACTG	ACTGCATCAAATGAATCTGCT	20	21	55.0	38.1	57.7	56.2	0.0	8.5	3.103150	111056	111414	409-415	1.000000	59.3
ODF51_pgt021-psbM_p3	ODF51_pgt021-psbM	3	GAGCAAGGAGGGATTCGAAC	ACTGCATCAAATGAATCTGCT	20	21	55.0	38.1	58.3	56.2	0.0	8.5	3.138986	111056	111414	450-456	1.000000	58.6
ODF51_pgt021-psbM_p4	ODF51_pgt021-psbM	4	TTTGAGCAAGGAGGGATTCG	AACTGCATCAAATGAATCTGCT	20	22	50.0	36.4	57.9	56.9	0.0	8.5	3.181519	111056	111414	454-460	1.000000	58.2
ODF51_pgt021-psbM_p5	ODF51_pgt021-psbM	5	TTTGAGCAAGGAGGGATTCG	ACTGCATCAAATGAATCTGCTT	20	22	50.0	36.4	57.9	56.9	0.0	8.5	3.181519	111056	111414	453-459	1.000000	58.2
ccsA-psaB_p1	ccsA-psaB	1	ACATGAAAAGCAATTTGAACCCA	TGTTCAAGCTCGTTTAGTAGGT	23	22	34.8	40.9	58.0	57.4	0.0	0.0	5.629351	114047	114830	948-982	1.000000	45.0
ccsA-psaB_p2	ccsA-psaB	2	ACATGAAAAGCAATTTGAACCCA	AGCTCGTTTAGTAGGTTTAGCA	23	22	34.8	40.9	58.0	57.2	0.0	0.0	5.829627	114047	114830	942-976	1.000000	45.0
ccsA-psaB_p3	ccsA-psaB	3	ACATGAAAAGCAATTTGAACCCA	GCTCGTTTAGTAGGTTTAGCAC	23	22	34.8	45.5	58.0	57.2	0.0	0.0	5.870520	114047	114830	941-975	1.000000	45.0
ccsA-psaB_p4	ccsA-psaB	4	ACATGAAAAGCAATTTGAACCCA	ACCTGTTGCGTTATCAATTGT	23	21	34.8	38.1	58.0	56.4	0.0	5.1	5.593316	114047	114830	966-1000	1.000000	44.9
ccsA-psaB_p5	ccsA-psaB	5	ACATGAAAAGCAATTTGAACCCA	TGCGTTATCAATTGTTCAAGCT	23	22	34.8	36.4	58.0	57.2	0.0	5.1	5.798267	114047	114830	960-994	1.000000	44.9
ODF51_pgt022-ODF51_pgt023_p1	ODF51_pgt022-ODF51_pgt023	1	TTCGAATCCCCGTTTTCTCC	GTCGTGGGTTCAAGTCCTAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.179516	120289	120847	628-646	1.000000	84.3
ODF51_pgt022-ODF51_pgt023_p2	ODF51_pgt022-ODF51_pgt023	2	TTCGAATCCCCGTTTTCTCC	CGTGGGTTCAAGTCCTACAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.183823	120289	120847	626-644	1.000000	84.3
ODF51_pgt022-ODF51_pgt023_p3	ODF51_pgt022-ODF51_pgt023	3	TTCGAATCCCCGTTTTCTCC	AAACCTGATGTCGTGGGTTC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.211328	120289	120847	637-655	1.000000	84.0
ODF51_pgt022-ODF51_pgt023_p4	ODF51_pgt022-ODF51_pgt023	4	TTCGAATCCCCGTTTTCTCC	TCCAAAACCTGATGTCGTGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.212661	120289	120847	641-659	1.000000	84.0
ODF51_pgt022-ODF51_pgt023_p5	ODF51_pgt022-ODF51_pgt023	5	TTCGAATCCCCGTTTTCTCC	GGTAGAACGCAGGTTTCCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.216651	120289	120847	656-674	1.000000	83.8
ODF51_pgt024_p1	ODF51_pgt024	1	TCTCAAACTCTTTCCAAGGGAC	ACAACTTTGTGAAGTAGAAAAAGGT	22	25	45.5	32.0	57.9	57.5	0.0	0.0	7.549277	121124	121196	184-184	1.000000	41.7
ODF51_pgt024_p2	ODF51_pgt024	2	TCTCAAACTCTTTCCAAGGGA	ACAACTTTGTGAAGTAGAAAAAGGT	21	25	42.9	32.0	56.5	57.5	0.0	0.0	7.980429	121124	121196	184-184	1.000000	41.7
ODF51_pgt024_p3	ODF51_pgt024	3	CTCAAACTCTTTCCAAGGGAC	ACAACTTTGTGAAGTAGAAAAAGGT	21	25	47.6	32.0	56.5	57.5	0.0	0.0	8.010812	121124	121196	183-183	1.000000	41.7
ODF51_pgt024_p4	ODF51_pgt024	4	TCTCAAACTCTTTCCAAGGGAC	ACAACTTTGTGAAGTAGAAAAAGG	22	24	45.5	33.3	57.9	56.0	0.0	0.0	8.088222	121124	121196	184-184	1.000000	41.7
ODF51_pgt024_p5	ODF51_pgt024	5	TCTCAAACTCTTTCCAAGGGAC	CAACTTTGTGAAGTAGAAAAAGGT	22	24	45.5	33.3	57.9	56.0	0.0	0.0	8.088222	121124	121196	183-183	1.000000	41.7