primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4290	5049	386-823	1.000000	90.0
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4290	5049	385-822	1.000000	90.0
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	TCTAGCCGCACTTAAAAGCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.3	58.1	0.0	16.9	0.341938	4290	5049	400-837	1.000000	89.3
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	CCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.3	58.1	5.3	16.9	0.358907	4290	5049	395-832	1.000000	89.1
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	AAGCCGAGTACTCTACCGTT	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	20.3	16.9	0.379772	4290	5049	389-826	1.000000	89.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.640998	6430	7127	792-863	1.000000	76.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.642386	6430	7127	791-862	1.000000	76.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AAACCGAAAGACCCCTTAACT	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.875121	6430	7127	793-864	1.000000	74.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	AAACCGAAAGACCCCTTAACT	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.876509	6430	7127	792-863	1.000000	74.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGCAATCCGACGCTTTAGTC	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.4	56.4	0.0	0.0	1.994839	6430	7127	794-865	1.000000	74.0
atpF-atpH_p1	atpF-atpH	1	CGACCCAAGGAAACGAAAGA	AATTTACGGACTGGTCGTGG	20	20	50.0	50.0	58.1	57.9	0.0	7.6	0.215252	11193	11615	501-519	1.000000	89.0
atpF-atpH_p2	atpF-atpH	2	TTAAATCCGAAACTCCCGGC	AATTTACGGACTGGTCGTGG	20	20	50.0	50.0	58.3	57.9	0.0	7.6	0.347421	11193	11615	531-546	0.961538	85.0
atpF-atpH_p3	atpF-atpH	3	CGACCCAAGGAAACGAAAGA	ACAATTTACGGACTGGTCGT	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.952163	11193	11615	503-521	1.000000	83.1
atpF-atpH_p4	atpF-atpH	4	CGACCCAAGGAAACGAAAGA	GGTCGTGGCATTAGCACTTT	20	20	50.0	50.0	58.1	58.8	0.0	0.0	0.961033	11193	11615	489-507	1.000000	83.0
atpF-atpH_p5	atpF-atpH	5	TTAAATCCGAAACTCCCGGC	ACAATTTACGGACTGGTCGT	20	20	50.0	45.0	58.3	57.2	0.0	0.0	1.084333	11193	11615	533-548	0.961538	79.1
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.746530	25057	26157	1132-1272	0.961538	81.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCTCATTTCCATCCCG	TTTCTTGATCAGGCGACACC	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.855991	25057	26157	1151-1291	0.961538	81.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.3	58.4	0.0	14.6	1.019893	25057	26157	1114-1254	0.961538	79.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	21	20	47.6	50.0	57.7	58.1	0.0	0.0	1.401159	25057	26157	1134-1274	0.961538	76.6
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ATTCCCTCATTTCCATCCCG	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	57.3	58.8	0.0	0.7	1.499132	25057	26157	1137-1277	0.961538	75.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	26229	27114	930-959	1.000000	88.7
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	26229	27114	924-953	1.000000	87.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTATCGTTTTGGCGGCATG	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	57.7	58.0	14.3	0.0	0.276319	26229	27114	983-1012	1.000000	86.7
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	26229	27114	948-977	1.000000	86.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	26229	27114	984-1013	1.000000	85.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.349607	31129	32681	1628-1739	1.000000	88.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	57.9	57.7	0.0	9.5	0.362803	31129	32681	1642-1753	1.000000	88.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	ACAGGCCCTTTTAACTCAGC	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.335012	31129	32681	1632-1743	0.923077	81.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	ACAGGCCCTTTTAACTCAGC	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	58.1	57.7	0.0	9.5	0.348209	31129	32681	1646-1757	0.923077	81.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	ACAGGCCCTTTTAACTCAGC	TAGACCGGACCAACCTACAA	20	20	50.0	50.0	58.1	57.7	0.0	9.5	0.396439	31129	32681	1648-1759	0.923077	81.2
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	44264	45130	945-962	1.000000	79.0
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	44264	45130	916-933	1.000000	78.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	44264	45130	966-983	1.000000	78.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	44264	45130	946-963	1.000000	76.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.6	58.1	8.2	0.0	1.514239	44264	45130	943-960	1.000000	75.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46597	47294	710-797	1.000000	89.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TAGACAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	33.1	0.0	0.288386	46597	47294	761-848	1.000000	88.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46597	47294	702-789	0.923077	86.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TAGACAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	33.1	0.0	0.223577	46597	47294	753-840	0.923077	85.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46597	47294	734-821	0.923077	85.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGCCAAAACTCCGGAAATT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	59.0	57.8	5.2	0.0	1.223651	50727	51796	1007-1236	1.000000	80.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATTGGGGCCAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	4.4	0.0	0.645831	50727	51796	1013-1226	0.923077	78.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TAATTGGGGCCAAAACTCCG	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.4	57.5	4.4	0.0	0.989603	50727	51796	1012-1225	0.923077	75.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAATTGGGGCCAAAACTCCG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	58.4	58.7	4.4	0.0	1.131897	50727	51796	1001-1214	0.923077	74.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TAATTGGGGCCAAAACTCCG	AAGGTCTACGGTTCGAGTCC	20	20	50.0	55.0	58.4	58.8	4.4	0.0	1.276373	50727	51796	1011-1224	0.923077	73.5
rbcL-accD_p1	rbcL-accD	1	TGCAAATGGAGCCTTGAACT	TAGCCCACACCTGTGTTCTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.018707	57000	57705	832-858	0.961538	86.1
rbcL-accD_p2	rbcL-accD	2	AATGGAGCCTTGAACTAGCC	TAGCCCACACCTGTGTTCTA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.445198	57000	57705	828-854	0.961538	82.7
rbcL-accD_p3	rbcL-accD	3	TGCAAATGGAGCCTTGAACT	CTTAGCCCACACCTGTGTTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.476594	57000	57705	834-860	0.961538	82.4
rbcL-accD_p4	rbcL-accD	4	TGCAAATGGAGCCTTGAACT	GGGACCAATACCTTCTAGCG	20	20	45.0	55.0	58.0	57.5	0.0	0.0	0.528393	57000	57705	859-885	0.961538	82.4
rbcL-accD_p5	rbcL-accD	5	TGCAAATGGAGCCTTGAACT	TACTTAGCCCACACCTGTGT	20	20	45.0	50.0	58.0	58.3	0.0	4.8	0.275807	57000	57705	836-862	0.923077	78.9
petA-psbJ_p1	petA-psbJ	1	TGGCATCTGTTATTCTGGCA	ATTCTGGGTTGGGTTCATCC	20	20	45.0	50.0	57.2	57.5	0.0	0.0	1.337236	63449	63891	546-558	0.961538	77.8
petA-psbJ_p2	petA-psbJ	2	TTGGCATCTGTTATTCTGGCA	ATTCTGGGTTGGGTTCATCC	21	20	42.9	50.0	57.9	57.5	0.0	0.0	1.616786	63449	63891	547-559	0.961538	75.5
petA-psbJ_p3	petA-psbJ	3	GGCATCTGTTATTCTGGCAC	ATTCTGGGTTGGGTTCATCC	20	20	50.0	50.0	56.9	57.5	0.0	0.0	1.675848	63449	63891	545-557	0.961538	75.1
petA-psbJ_p4	petA-psbJ	4	TGGCATCTGTTATTCTGGCA	TGGCTTATAGGCACTGTAGC	20	20	45.0	50.0	57.2	57.1	0.0	9.3	1.715674	63449	63891	613-625	0.961538	73.8
petA-psbJ_p5	petA-psbJ	5	TGGCATCTGTTATTCTGGCA	GGCACTGTAGCTGGTATTCT	20	20	45.0	50.0	57.2	57.0	0.0	0.0	1.795052	63449	63891	604-616	0.961538	73.3
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AATGTCAACGCATCTGGGAA	20	20	50.0	45.0	58.1	57.8	0.0	0.8	0.298581	66663	67350	80-903	1.000000	89.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	AATGTCAACGCATCTGGGAA	20	20	50.0	45.0	58.1	57.8	0.0	0.8	0.299925	66663	67350	80-902	1.000000	89.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AATGTCAACGCATCTGGGAA	20	20	50.0	45.0	58.1	57.8	0.0	0.8	0.306617	66663	67350	80-914	1.000000	89.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	AATGTCAACGCATCTGGGAA	20	20	50.0	45.0	58.1	57.8	0.0	0.8	0.307951	66663	67350	80-913	1.000000	89.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	AATGTCAACGCATCTGGGAA	20	20	50.0	45.0	58.1	57.8	0.0	0.8	0.307951	66663	67350	82-916	1.000000	89.5
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACGCCTAGTCG	AATCAATTCGATCCCCCGAC	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.792312	68084	68256	343-1380	1.000000	83.4
rpl33-rps18_p2	rpl33-rps18	2	GCATACGATTCATGGGGAGA	AATCAATTCGATCCCCCGAC	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.095603	68084	68256	287-1378	1.000000	81.6
rpl33-rps18_p3	rpl33-rps18	3	AGCATACGATTCATGGGGAG	AATCAATTCGATCCCCCGAC	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.103444	68084	68256	288-1378	1.000000	81.5
rpl33-rps18_p4	rpl33-rps18	4	GACACAATACGCCTAGTCGA	AATCAATTCGATCCCCCGAC	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.094504	68084	68256	342-1379	1.000000	81.0
rpl33-rps18_p5	rpl33-rps18	5	TCGACACAATACGCCTAGTC	AATCAATTCGATCCCCCGAC	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.094504	68084	68256	344-1378	1.000000	81.0
rps8-rpl14_p1	rps8-rpl14	1	AGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	20	20	50.0	50.0	57.7	58.0	0.0	30.2	0.282612	80917	81160	334-371	0.923077	83.0
rps8-rpl14_p2	rps8-rpl14	2	AGCAATAGTGTCCCTACCCA	GCTCGGGAATTGAGACAGTT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.364500	80917	81160	311-348	0.923077	82.6
rps8-rpl14_p3	rps8-rpl14	3	AGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	20	20	50.0	45.0	57.7	58.1	0.0	13.6	0.384942	80917	81160	332-369	0.923077	82.2
rps8-rpl14_p4	rps8-rpl14	4	AGCAATAGTGTCCCTACCCA	AACTCGAGTTTTTGGTGCGA	20	20	50.0	45.0	57.7	58.3	0.0	24.9	0.604901	80917	81160	333-370	0.923077	80.4
rps8-rpl14_p5	rps8-rpl14	5	CAGCAATAGTGTCCCTACCC	GAACTCGAGTTTTTGGTGCG	20	20	55.0	50.0	57.4	58.0	0.0	30.2	0.640781	80917	81160	335-372	0.923077	80.1
rpl16_p1	rpl16	1	TTTTTCACAGGGCCCCTTTT	TGACTCCATATACCATATAATTGCT	20	25	45.0	32.0	58.1	55.5	21.4	0.0	7.638457	81652	83180	1584-1607	0.269231	11.9
rpl16_p2	rpl16	2	TTTTTCACAGGGCCCCTTTT	TTGACTCCATATACCATATAATTGCT	20	26	45.0	30.8	58.1	56.1	21.4	0.0	7.978569	81652	83180	1585-1608	0.269231	11.9
rpl16_p3	rpl16	3	TTTTTCACAGGGCCCCTTTT	TGACTCCATATACCATATAATTGC	20	24	45.0	33.3	58.1	54.1	21.4	0.0	8.005325	81652	83180	1584-1607	0.269231	11.9
rpl16_p4	rpl16	4	TTTTCACAGGGCCCCTTTTC	TGACTCCATATACCATATAATTGCT	20	25	50.0	32.0	58.6	55.5	21.4	0.0	8.105499	81652	83180	1583-1606	0.269231	11.9
rpl16_p5	rpl16	5	TTTTTCACAGGGCCCCTTTT	GACTCCATATACCATATAATTGCT	20	24	45.0	33.3	58.1	53.8	21.4	0.0	8.268794	81652	83180	1583-1606	0.269231	11.9
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	GGTATGATCCACGCATATTGA	AGCGTGTCTACCGATTTCAC	21	20	42.9	50.0	55.6	58.0	0.0	0.0	3.380041	113625	114485	660-953	1.000000	65.0
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	GGTATGATCCACGCATATTGA	CAGCGTGTCTACCGATTTCA	21	20	42.9	50.0	55.6	58.0	0.0	0.0	3.381412	113625	114485	661-954	1.000000	64.9
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	GGTATGATCCACGCATATTGA	TCCTAAGAGCAGCGTGTCTA	21	20	42.9	50.0	55.6	57.9	0.0	0.0	3.496488	113625	114485	670-963	1.000000	64.0
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	GGTATGATCCACGCATATTGA	TTATAAGATGCCGCCACTCG	21	20	42.9	50.0	55.6	57.9	0.0	0.0	3.521126	113625	114485	719-1012	1.000000	63.6
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	GGTATGATCCACGCATATTGA	CTAAGAGCAGCGTGTCTACC	21	20	42.9	55.0	55.6	57.8	0.0	0.0	3.568506	113625	114485	668-961	1.000000	63.5
ycf1_p1	ycf1	1	TGGCTATTATCAACAAAGGCA	ACGATTATGGCGAGAAAGGG	21	20	38.1	50.0	55.5	57.8	0.0	0.0	3.695364	127127	127246	192-207	1.000000	61.3
ycf1_p2	ycf1	2	TGGCTATTATCAACAAAGGCA	AAGGGCGCGGAATTAAAGAT	21	20	38.1	45.0	55.5	57.9	0.0	8.8	3.507653	127127	127246	214-223	0.923077	59.4
ycf1_p3	ycf1	3	TGGCTATTATCAACAAAGGCA	AGGGCGCGGAATTAAAGATT	21	20	38.1	45.0	55.5	57.9	0.0	8.8	3.507653	127127	127246	213-222	0.923077	59.4
ycf1_p4	ycf1	4	TGGCTATTATCAACAAAGGCAA	AAGGGCGCGGAATTAAAGAT	22	20	36.4	45.0	56.3	57.9	0.0	8.8	3.742889	127127	127246	214-223	0.923077	57.5
ycf1_p5	ycf1	5	TGGCTATTATCAACAAAGGCAA	AGGGCGCGGAATTAAAGATT	22	20	36.4	45.0	56.3	57.9	0.0	8.8	3.742889	127127	127246	213-222	0.923077	57.5