primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	rps16	1	TCATGATTCTTGTGTTCCCT	TGGTTCAGTAAATGACTATTCATGA	20	25	40.0	32.0	54.2	56.3	0.0	0.0	10.475995	3844	4896	1183-1189	1.000000	40.7
rps16_p2	rps16	2	TCATGATTCTTGTGTTCCCTT	TGGTTCAGTAAATGACTATTCATGA	21	25	38.1	32.0	55.1	56.3	0.0	0.0	10.607911	3844	4896	1183-1189	1.000000	40.7
rps16_p3	rps16	3	TTCATGATTCTTGTGTTCCCT	TGGTTCAGTAAATGACTATTCATGA	21	25	38.1	32.0	55.1	56.3	0.0	0.0	10.607911	3844	4896	1184-1190	1.000000	40.7
rps16_p4	rps16	4	TCATGATTCTTGTGTTCCCT	TGTATTGGTTCAGTAAATGACT	20	22	40.0	31.8	54.2	53.1	0.0	0.0	10.661870	3844	4896	1188-1194	1.000000	40.7
rps16_p5	rps16	5	TCATGATTCTTGTGTTCCCTTTC	TGGTTCAGTAAATGACTATTCATGA	23	25	39.1	32.0	57.0	56.3	0.0	0.0	10.675440	3844	4896	1183-1189	1.000000	40.7
rps2-trnC-GCA_p1	rps2-trnC-GCA	1	GAGCACTCGAGCAGTTTCTT	ACGTCAAGTAACACCAAGAGT	20	21	50.0	42.9	58.2	57.2	13.6	0.0	2.024499	8637	8756	217-217	1.000000	65.3
rps2-trnC-GCA_p2	rps2-trnC-GCA	2	TGTTGAATCCGGTACCAACC	ACGTCAAGTAACACCAAGAGT	20	21	50.0	42.9	57.8	57.2	22.6	0.0	2.002067	8637	8756	302-302	1.000000	65.0
rps2-trnC-GCA_p3	rps2-trnC-GCA	3	CCAACCGGTATAATTCCCCC	ACGTCAAGTAACACCAAGAGT	20	21	55.0	42.9	57.7	57.2	8.0	0.0	2.095544	8637	8756	288-288	1.000000	64.3
rps2-trnC-GCA_p4	rps2-trnC-GCA	4	GTAGAGCACTCGAGCAGTTT	ACGTCAAGTAACACCAAGAGT	20	21	50.0	42.9	57.6	57.2	13.6	0.0	2.169722	8637	8756	220-220	1.000000	64.1
rps2-trnC-GCA_p5	rps2-trnC-GCA	5	AGCACTCGAGCAGTTTCTTG	ACGTCAAGTAACACCAAGAGT	20	21	50.0	42.9	58.5	57.2	13.6	0.0	2.307512	8637	8756	216-216	1.000000	63.0
trnC-GCA-trnE-UUC_p1	trnC-GCA-trnE-UUC	1	CCAGTTCGAATCTGGGTGTT	GTCGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	57.8	58.0	26.3	0.0	0.188200	11296	12848	1625-1690	1.000000	82.6
trnC-GCA-trnE-UUC_p2	trnC-GCA-trnE-UUC	2	CCAGTTCGAATCTGGGTGTT	TCCACAGCCAACTCACTTTC	20	20	50.0	50.0	57.8	58.0	26.3	0.0	0.217686	11296	12848	1679-1744	1.000000	82.3
trnC-GCA-trnE-UUC_p3	trnC-GCA-trnE-UUC	3	CCAGTTCGAATCTGGGTGTT	TCGCCTCCTTGAAAGAGAGA	20	20	50.0	50.0	57.8	58.1	26.3	9.0	0.260273	11296	12848	1624-1689	1.000000	82.1
trnC-GCA-trnE-UUC_p4	trnC-GCA-trnE-UUC	4	CCAGTTCGAATCTGGGTGTT	TGTCCTGAACCACTAGACGA	20	20	50.0	50.0	57.8	57.7	26.3	0.0	0.466543	11296	12848	1604-1669	1.000000	80.5
trnC-GCA-trnE-UUC_p5	trnC-GCA-trnE-UUC	5	AGTTCGAATCTGGGTGTTGC	GTCGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	58.5	58.0	0.0	0.0	0.479439	11296	12848	1623-1688	1.000000	80.3
trnD-GUC-petN_p1	trnD-GUC-petN	1	AGTTCAATCGGTCAGAGCAC	TAGTAAGTCTCGCTTGGGCT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.237403	13447	14432	1092-1135	1.000000	81.6
trnD-GUC-petN_p2	trnD-GUC-petN	2	AGTTCAATCGGTCAGAGCAC	TAGTATGGGGAAGGAGTGGG	20	20	50.0	55.0	57.9	57.5	0.0	0.0	0.575582	13447	14432	1035-1078	1.000000	79.1
trnD-GUC-petN_p3	trnD-GUC-petN	3	AGTTCAATCGGTCAGAGCAC	ATAGTAAGTCTCGCTTGGGC	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.978839	13447	14432	1093-1136	1.000000	75.7
trnD-GUC-petN_p4	trnD-GUC-petN	4	GTTCAATCGGTCAGAGCACC	TAGTAAGTCTCGCTTGGGCT	20	20	55.0	50.0	58.9	58.2	0.0	0.0	1.071845	13447	14432	1091-1134	1.000000	74.9
trnD-GUC-petN_p5	trnD-GUC-petN	5	AGTTCAATCGGTCAGAGCAC	TCGCTTGGGCTGCTTTAAA	20	19	50.0	47.4	57.9	58.0	0.0	0.0	1.114970	13447	14432	1083-1126	1.000000	74.6
trnG-GCC_p1	trnG-GCC	1	ACGCATCAGGAACGAAGAAA	AGTCAACTATACTGACAGAATCT	20	23	45.0	34.8	57.8	54.3	0.0	10.1	6.858319	14978	15042	125-125	1.000000	40.6
trnG-GCC_p2	trnG-GCC	2	ACGCATCAGGAACGAAGAAA	ACTACTGTATTCACTACTATGCA	20	23	45.0	34.8	57.8	54.4	0.0	0.0	6.768941	14978	15042	164-164	1.000000	40.5
trnG-GCC_p3	trnG-GCC	3	TATGTGTGGACATATGCGGC	AGTCAACTATACTGACAGAATCT	20	23	50.0	34.8	58.1	54.3	1.6	10.1	6.816865	14978	15042	147-147	1.000000	40.5
trnG-GCC_p4	trnG-GCC	4	GTACGCATCAGGAACGAAGA	ACTACTGTATTCACTACTATGCA	20	23	50.0	34.8	57.7	54.4	0.0	0.0	6.878493	14978	15042	166-166	1.000000	40.5
trnG-GCC_p5	trnG-GCC	5	TATGTGTGGACATATGCGGC	ACTACTGTATTCACTACTATGCA	20	23	50.0	34.8	58.1	54.4	1.6	0.0	6.727486	14978	15042	186-186	1.000000	40.4
trnL-UAA_p1	trnL-UAA	1	GACGAGAATAAAGAGAGAGTCCC	AGCGAGGAAGTAAAATGGGC	23	20	47.8	50.0	57.8	58.2	0.0	0.0	3.426288	21752	21801	145-145	1.000000	53.7
trnL-UAA_p2	trnL-UAA	2	GACGAGAATAAAGAGAGAGTCCC	GCGAGGAAGTAAAATGGGCT	23	20	47.8	50.0	57.8	58.2	0.0	0.0	3.426288	21752	21801	144-144	1.000000	53.7
trnL-UAA_p3	trnL-UAA	3	GGACGAGAATAAAGAGAGAGTCC	AGCGAGGAAGTAAAATGGGC	23	20	47.8	50.0	57.8	58.2	0.0	0.0	3.426288	21752	21801	146-146	1.000000	53.7
trnL-UAA_p4	trnL-UAA	4	GGACGAGAATAAAGAGAGAGTCC	GCGAGGAAGTAAAATGGGCT	23	20	47.8	50.0	57.8	58.2	0.0	0.0	3.426288	21752	21801	145-145	1.000000	53.7
trnL-UAA_p5	trnL-UAA	5	CGAGAATAAAGAGAGAGTCCCA	GCGAGGAAGTAAAATGGGCT	22	20	45.5	50.0	56.7	58.2	0.0	0.0	3.505602	21752	21801	142-142	1.000000	53.1
accD_p1	accD	1	ATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCT	20	22	45.0	40.9	55.6	56.8	0.0	0.0	5.658717	26066	27547	1604-1622	1.000000	41.6
accD_p2	accD	2	AATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCT	21	22	42.9	40.9	56.4	56.8	0.0	0.0	5.840718	26066	27547	1605-1623	1.000000	41.6
accD_p3	accD	3	ATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCTT	20	23	45.0	39.1	55.6	57.5	0.0	0.0	5.984396	26066	27547	1604-1622	1.000000	41.6
accD_p4	accD	4	AAATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTCT	22	22	40.9	40.9	57.1	56.8	0.0	0.0	6.098700	26066	27547	1606-1624	1.000000	41.6
accD_p5	accD	5	ATAGGGGGCAGAAAGAATCT	CCCAAAGAAAACACCATCTTC	20	21	45.0	42.9	55.6	55.3	0.0	0.0	6.145270	26066	27547	1604-1622	1.000000	41.6
clpP-rps11_p1	clpP-rps11	1	TGGACCCATATGTCTGACGA	AAGAGATGCGGCATTACGAG	20	20	50.0	50.0	57.8	58.1	3.0	17.8	0.294942	35854	38290	2650-2913	1.000000	79.6
clpP-rps11_p2	clpP-rps11	2	TGGACCCATATGTCTGACGA	GAAGAGATGCGGCATTACGA	20	20	50.0	50.0	57.8	58.1	3.0	17.8	0.300092	35854	38290	2651-2914	1.000000	79.6
clpP-rps11_p3	clpP-rps11	3	TGGACCCATATGTCTGACGA	GATGTAACACCCATGCCACA	20	20	50.0	50.0	57.8	58.2	3.0	0.0	0.320150	35854	38290	2592-2855	1.000000	79.5
clpP-rps11_p4	clpP-rps11	4	TGGACCCATATGTCTGACGA	GTGATGTAACACCCATGCCA	20	20	50.0	50.0	57.8	58.2	3.0	0.0	0.320150	35854	38290	2594-2857	1.000000	79.5
clpP-rps11_p5	clpP-rps11	5	TGGACCCATATGTCTGACGA	TGATGTAACACCCATGCCAC	20	20	50.0	50.0	57.8	58.2	3.0	0.0	0.320150	35854	38290	2593-2856	1.000000	79.5
rpl16_p1	rpl16	1	AAAGAAATCCGTGGCTGTTT	AACGAACAATTATATAAGGTTGAA	20	24	40.0	25.0	56.1	52.8	0.0	16.0	11.066400	40650	42325	1807-1808	1.000000	40.6
rpl16_p2	rpl16	2	AAGAAATCCGTGGCTGTTTT	AAACGAACAATTATATAAGGTTGA	20	24	40.0	25.0	56.1	52.8	0.0	16.0	11.066400	40650	42325	1807-1808	1.000000	40.6
rpl16_p3	rpl16	3	AAGAAATCCGTGGCTGTTTT	AACGAACAATTATATAAGGTTGAA	20	24	40.0	25.0	56.1	52.8	0.0	16.0	11.066400	40650	42325	1806-1807	1.000000	40.6
rpl16_p4	rpl16	4	AGAAATCCGTGGCTGTTTTT	AAACGAACAATTATATAAGGTTGA	20	24	40.0	25.0	56.1	52.8	0.0	16.0	11.066400	40650	42325	1806-1807	1.000000	40.6
rpl16_p5	rpl16	5	AGAAATCCGTGGCTGTTTTT	AACGAACAATTATATAAGGTTGAA	20	24	40.0	25.0	56.1	52.8	0.0	16.0	11.066400	40650	42325	1805-1806	1.000000	40.6
rps3-rpl22_p1	rps3-rpl22	1	CCGAGTCTGAAACCAAGTGG	TCGAGGGCGTAGTTATCTCA	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.824019	43154	43196	157-157	1.000000	73.9
rps3-rpl22_p2	rps3-rpl22	2	CGAGTCTGAAACCAAGTGGA	TCGAGGGCGTAGTTATCTCA	20	20	50.0	50.0	57.5	57.7	3.8	0.0	0.863249	43154	43196	156-156	1.000000	73.6
rps3-rpl22_p3	rps3-rpl22	3	TCCGAGTCTGAAACCAAGTG	TCGAGGGCGTAGTTATCTCA	20	20	50.0	50.0	57.5	57.7	0.0	0.0	0.863249	43154	43196	158-158	1.000000	73.6
rps3-rpl22_p4	rps3-rpl22	4	CCGAGTCTGAAACCAAGTGG	AAAGTTAAGACCCCGAGCTC	20	20	55.0	50.0	58.5	57.2	0.0	2.7	1.251513	43154	43196	175-175	1.000000	70.4
rps3-rpl22_p5	rps3-rpl22	5	CGAGTCTGAAACCAAGTGGA	AAAGTTAAGACCCCGAGCTC	20	20	50.0	50.0	57.5	57.2	3.8	2.7	1.290742	43154	43196	174-174	1.000000	70.1
psaC-rps7_p1	psaC-rps7	1	ACATCCGTCCCAAGGTATCA	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147416	54842	56048	1185-1348	1.000000	83.3
psaC-rps7_p2	psaC-rps7	2	TTCCGATACATCTGTGGGGT	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147416	54842	56048	1164-1327	1.000000	83.3
psaC-rps7_p3	psaC-rps7	3	ACATCCGTCCCAAGGTATCA	GGCAGAGGCAAATAGAGCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.175759	54842	56048	1183-1346	1.000000	83.1
psaC-rps7_p4	psaC-rps7	4	TTCCGATACATCTGTGGGGT	GGCAGAGGCAAATAGAGCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.175759	54842	56048	1162-1325	1.000000	83.1
psaC-rps7_p5	psaC-rps7	5	CGGACACATTGGGTACATCC	ATGGCAGAGGCAAATAGAGC	20	20	55.0	50.0	58.3	58.0	1.4	0.0	0.357851	54842	56048	1139-1302	1.000000	81.7
rps7-rps12_p1	rps7-rps12	1	TTCTTCTGCAGTACCTCGAC	CTGTGGAAAGCCGTATTCGA	20	20	50.0	50.0	57.0	58.0	14.7	0.0	1.016277	56517	56574	190-190	1.000000	72.3
rps7-rps12_p2	rps7-rps12	2	TTCTTCTGCAGTACCTCGAC	TCTGTGGAAAGCCGTATTCG	20	20	50.0	50.0	57.0	58.0	14.7	0.0	1.016277	56517	56574	191-191	1.000000	72.3
rps7-rps12_p3	rps7-rps12	3	TCTTCTGCAGTACCTCGACG	CTGTGGAAAGCCGTATTCGA	20	20	55.0	50.0	59.2	58.0	14.7	0.0	1.201466	56517	56574	189-189	1.000000	70.8
rps7-rps12_p4	rps7-rps12	4	TCTTCTGCAGTACCTCGACG	TCTGTGGAAAGCCGTATTCG	20	20	55.0	50.0	59.2	58.0	14.7	0.0	1.201466	56517	56574	190-190	1.000000	70.8
rps7-rps12_p5	rps7-rps12	5	TTTCTTCTGCAGTACCTCGAC	CTGTGGAAAGCCGTATTCGA	21	20	47.6	50.0	57.7	58.0	14.7	0.0	1.303923	56517	56574	191-191	1.000000	70.0
trnV-GAC-rrn16S_p1	trnV-GAC-rrn16S	1	ATTTCTCGATGGGACTGGGA	TCCGACTCGCATGTGTTAAG	20	20	50.0	50.0	58.1	58.0	0.0	6.1	0.129626	58855	59067	371-372	1.000000	79.8
trnV-GAC-rrn16S_p2	trnV-GAC-rrn16S	2	ATTTCTCGATGGGACTGGGA	CCGGGATCAAACTCTCCATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.161384	58855	59067	327-328	1.000000	79.7
trnV-GAC-rrn16S_p3	trnV-GAC-rrn16S	3	TTTCTCGATGGGACTGGGAA	TCCGACTCGCATGTGTTAAG	20	20	50.0	50.0	58.3	58.0	0.0	6.1	0.357464	58855	59067	370-371	1.000000	78.0
trnV-GAC-rrn16S_p4	trnV-GAC-rrn16S	4	TTTCTCGATGGGACTGGGAA	CCGGGATCAAACTCTCCATG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.389222	58855	59067	326-327	1.000000	77.9
trnV-GAC-rrn16S_p5	trnV-GAC-rrn16S	5	ATTTCTCGATGGGACTGGGA	GCAGGTTCTTACGCGTTACT	20	20	50.0	50.0	58.1	58.3	0.0	20.8	0.413982	58855	59067	422-423	1.000000	77.4
rrn4.5S-rrn5S_p1	rrn4.5S-rrn5S	1	GAGACGAGCCGTTTCTCATT	ACCAAGTTCGGGATGGATTG	20	20	50.0	50.0	58.0	57.9	9.8	0.0	0.131101	64553	64764	356-356	1.000000	79.6
rrn4.5S-rrn5S_p2	rrn4.5S-rrn5S	2	GAGACGAGCCGTTTCTCATT	CACCAAGTTCGGGATGGATT	20	20	50.0	50.0	58.0	57.9	9.8	0.0	0.131101	64553	64764	357-357	1.000000	79.6
rrn4.5S-rrn5S_p3	rrn4.5S-rrn5S	3	AAATAAGGTCACGGCGAGAC	ACCAAGTTCGGGATGGATTG	20	20	50.0	50.0	58.0	57.9	25.9	0.0	0.134741	64553	64764	371-371	1.000000	79.6
rrn4.5S-rrn5S_p4	rrn4.5S-rrn5S	4	AAATAAGGTCACGGCGAGAC	CACCAAGTTCGGGATGGATT	20	20	50.0	50.0	58.0	57.9	25.9	0.0	0.134741	64553	64764	372-372	1.000000	79.6
rrn4.5S-rrn5S_p5	rrn4.5S-rrn5S	5	GAGACGAGCCGTTTCTCATT	ACGCCTAGGACACCAGAATA	20	20	50.0	50.0	58.0	57.8	9.8	14.8	0.156898	64553	64764	323-323	1.000000	79.5
ycf1-rpl32_p1	ycf1-rpl32	1	AACAGCAGCCGTTTCAGTAT	TTCACCGGACAGTCAACAAG	20	20	45.0	50.0	57.8	58.1	0.0	9.5	0.236900	71497	73145	1047-1760	1.000000	82.3
ycf1-rpl32_p2	ycf1-rpl32	2	ATAACAGCAGCCGTTTCAGT	TTCACCGGACAGTCAACAAG	20	20	45.0	50.0	57.8	58.1	0.0	9.5	0.236900	71497	73145	1049-1762	1.000000	82.3
ycf1-rpl32_p3	ycf1-rpl32	3	CCAATAACAGCAGCCGTTTC	TTCACCGGACAGTCAACAAG	20	20	50.0	50.0	57.7	58.1	0.0	9.5	0.314468	71497	73145	1052-1765	1.000000	81.7
ycf1-rpl32_p4	ycf1-rpl32	4	AGCAGCCGTTTCAGTATGTC	TTCACCGGACAGTCAACAAG	20	20	50.0	50.0	58.3	58.1	0.0	9.5	0.325042	71497	73145	1044-1757	1.000000	81.6
ycf1-rpl32_p5	ycf1-rpl32	5	ACCAATAACAGCAGCCGTTT	TTCACCGGACAGTCAACAAG	20	20	45.0	50.0	58.4	58.1	0.0	9.5	0.435737	71497	73145	1053-1766	1.000000	80.7