primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	atpF-atpH	1	CCAGTAACCCAAGCAAAGGA	GGCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.536172	17605	18248	723-733	1.000000	79.6
atpF-atpH_p2	atpF-atpH	2	CCAGTAACCCAAGCAAAGGA	TGGGCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.704733	17605	18248	725-735	1.000000	78.2
atpF-atpH_p3	atpF-atpH	3	CCAGTAACCCAAGCAAAGGA	AGGCAGAGGGAAAAATACGG	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.706423	17605	18248	792-802	1.000000	77.9
atpF-atpH_p4	atpF-atpH	4	CCAGTAACCCAAGCAAAGGA	CCTGAGGCAGAGGGAAAAAT	20	20	50.0	50.0	57.7	57.5	0.0	0.0	0.807911	17605	18248	796-806	1.000000	77.0
atpF-atpH_p5	atpF-atpH	5	CCAGTAACCCAAGCAAAGGA	GCAGAGGGAAAAATACGGGG	20	20	50.0	55.0	57.7	58.6	0.0	0.0	0.896809	17605	18248	790-800	1.000000	76.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.0	57.8	0.0	1.1	0.185568	41144	41708	716-719	1.000000	81.9
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	41144	41708	719-722	1.000000	80.3
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.395214	41144	41708	712-715	1.000000	80.2
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	41144	41708	717-720	1.000000	79.9
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.530110	41144	41708	715-718	1.000000	79.1
ycf1_p1	ycf1	1	AACAGAATACGCGGATCGAG	AGCCAAAGCCTTACAGCAAT	20	20	50.0	45.0	57.9	58.1	12.5	0.0	0.192248	116077	116196	748-748	0.500000	58.5
ycf1_p2	ycf1	2	TAACCCGTGGATCATACCCA	AGCCAAAGCCTTACAGCAAT	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.248943	116077	116196	916-916	0.500000	58.0
ycf1_p3	ycf1	3	AACAGAATACGCGGATCGAG	CCTACGACGTCTAGCGGATA	20	20	50.0	55.0	57.9	58.2	12.5	14.7	0.332858	116077	116196	640-640	0.500000	57.3
ycf1_p4	ycf1	4	ACAGGCCTTTCGTTGATTCA	AGCCAAAGCCTTACAGCAAT	20	20	45.0	45.0	57.7	58.1	0.0	0.0	0.341484	116077	116196	300-300	0.500000	57.3
ycf1_p5	ycf1	5	TCACAGGCCTTTCGTTGATT	AGCCAAAGCCTTACAGCAAT	20	20	45.0	45.0	57.7	58.1	0.0	0.0	0.341484	116077	116196	302-302	0.500000	57.3
ycf1-rps15_p1	ycf1-rps15	1	ATTGCTGTAAGGCTTTGGCT	AACACCATGCATTGATGGGT	20	20	45.0	45.0	58.1	58.1	4.4	8.9	0.129755	116515	116928	1060-1060	0.500000	59.0
ycf1-rps15_p2	ycf1-rps15	2	ATTGCTGTAAGGCTTTGGCT	TGCCATAAAAGGTCCCAACC	20	20	45.0	50.0	58.1	58.1	4.4	0.0	0.139497	116515	116928	1354-1354	0.500000	58.9
ycf1-rps15_p3	ycf1-rps15	3	ATTGCTGTAAGGCTTTGGCT	ATTTCCCAACAGTTCCGGTC	20	20	45.0	50.0	58.1	58.1	4.4	0.0	0.165494	116515	116928	1258-1258	0.500000	58.7
ycf1-rps15_p4	ycf1-rps15	4	ATTGCTGTAAGGCTTTGGCT	CCGATCCGTGGTTTTCTTCT	20	20	45.0	50.0	58.1	57.9	4.4	0.0	0.165894	116515	116928	678-678	0.500000	58.7
ycf1-rps15_p5	ycf1-rps15	5	ATTGCTGTAAGGCTTTGGCT	TAATCGTTGCCGTTTTCCCA	20	20	45.0	45.0	58.1	58.1	4.4	0.0	0.177587	116515	116928	803-803	0.500000	58.6
rps15-ndhH_p1	rps15-ndhH	1	AAAACCACGGATCGGTTGAA	AACACCATGCATTGATGGGT	20	20	45.0	45.0	58.0	58.1	10.9	8.9	0.088531	117196	117302	397-397	0.500000	59.3
rps15-ndhH_p2	rps15-ndhH	2	AAAACCACGGATCGGTTGAA	TGCCATAAAAGGTCCCAACC	20	20	45.0	50.0	58.0	58.1	10.9	0.0	0.098273	117196	117302	691-691	0.500000	59.2
rps15-ndhH_p3	rps15-ndhH	3	AAAACCACGGATCGGTTGAA	ATTTCCCAACAGTTCCGGTC	20	20	45.0	50.0	58.0	58.1	10.9	0.0	0.124271	117196	117302	595-595	0.500000	59.0
rps15-ndhH_p4	rps15-ndhH	4	AAAACCACGGATCGGTTGAA	ATCGGTCCTGATAAACCCCA	20	20	45.0	50.0	58.0	58.1	10.9	0.0	0.142111	117196	117302	990-990	0.500000	58.9
rps15-ndhH_p5	rps15-ndhH	5	AGAAGAAAACCACGGATCGG	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.153385	117196	117302	402-402	0.500000	58.8
ndhH_p1	ndhH	1	AAAACCACGGATCGGTTGAA	CTAGGAAAGCGGCATAGCAA	20	20	45.0	50.0	58.0	58.0	10.9	1.4	0.076349	117303	118484	2113-2113	0.500000	59.4
ndhH_p2	ndhH	2	AAAACCACGGATCGGTTGAA	CGTTGTTGTATCCCTGCAGA	20	20	45.0	50.0	58.0	57.9	10.9	17.4	0.123214	117303	118484	1687-1687	0.500000	59.0
ndhH_p3	ndhH	3	AAAACCACGGATCGGTTGAA	AAGCGGCATAGCAATAGCAT	20	20	45.0	45.0	58.0	58.1	10.9	1.4	0.128317	117303	118484	2107-2107	0.500000	59.0
ndhH_p4	ndhH	4	AAAACCACGGATCGGTTGAA	GCTTGAAGAACTCCTCCAGG	20	20	45.0	55.0	58.0	57.9	10.9	0.0	0.136385	117303	118484	1729-1729	0.500000	58.9
ndhH_p5	ndhH	5	AGAAGAAAACCACGGATCGG	CTAGGAAAGCGGCATAGCAA	20	20	50.0	50.0	57.9	58.0	0.0	1.4	0.141203	117303	118484	2118-2118	0.500000	58.9
ndhA_p1	ndhA	1	AGAGTTGAAGCCCCAAAAGG	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.052972	118486	120786	2631-2631	0.500000	59.6
ndhA_p2	ndhA	2	TTCGTCCACCGGGTTTTATC	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	57.9	58.1	6.3	0.0	0.149575	118486	120786	2555-2555	0.500000	58.8
ndhA_p3	ndhA	3	TGGGGTTTATCAGGACCGAT	CTCGGACTGTTTGTTGACCA	20	20	50.0	50.0	58.1	58.1	4.6	0.0	0.164099	118486	120786	2967-2967	0.500000	58.7
ndhA_p4	ndhA	4	TCCGACTTTTGAATTGCCGA	CTCGGACTGTTTGTTGACCA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.166336	118486	120786	2668-2668	0.500000	58.7
ndhA_p5	ndhA	5	TTCGTCCACCGGGTTTTATC	CGGCCCAAAGCAATTTGATT	20	20	50.0	45.0	57.9	57.9	6.3	0.0	0.195359	118486	120786	2934-2934	0.500000	58.4
ndhI_p1	ndhI	1	ACAGTTCTTTACTTGGGCGG	GTGCTGCAGCTACAAAATGG	20	20	50.0	50.0	58.1	58.0	0.0	36.6	0.121093	120866	121348	1501-1501	0.500000	59.0
ndhI_p2	ndhI	2	CAGTTCTTTACTTGGGCGGT	GTGCTGCAGCTACAAAATGG	20	20	50.0	50.0	58.1	58.0	0.0	36.6	0.121093	120866	121348	1500-1500	0.500000	59.0
ndhI_p3	ndhI	3	ACAGTTCTTTACTTGGGCGG	CAAGAACCAGTCCCAACGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.145997	120866	121348	1447-1447	0.500000	58.8
ndhI_p4	ndhI	4	CAGTTCTTTACTTGGGCGGT	CAAGAACCAGTCCCAACGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.145997	120866	121348	1446-1446	0.500000	58.8
ndhI_p5	ndhI	5	CAGTTCTTTACTTGGGCGGT	GGACCAATGTCATTCCCCTC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.166383	120866	121348	1174-1174	0.500000	58.7
ndhI-ndhG_p1	ndhI-ndhG	1	TGGTCAACAAACAGTCCGAG	GTGCTGCAGCTACAAAATGG	20	20	50.0	50.0	58.1	58.0	0.0	36.6	0.065198	121349	121824	1111-1111	0.500000	59.5
ndhI-ndhG_p2	ndhI-ndhG	2	TGGTCAACAAACAGTCCGAG	CAAGAACCAGTCCCAACGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.090102	121349	121824	1057-1057	0.500000	59.3
ndhI-ndhG_p3	ndhI-ndhG	3	AATCAAATTGCTTTGGGCCG	GTGCTGCAGCTACAAAATGG	20	20	45.0	50.0	57.9	58.0	0.0	36.6	0.110982	121349	121824	732-732	0.500000	59.1
ndhI-ndhG_p4	ndhI-ndhG	4	TGGTCAACAAACAGTCCGAG	AACGGTTCTGGCAAATCCAT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.128540	121349	121824	947-947	0.500000	59.0
ndhI-ndhG_p5	ndhI-ndhG	5	TGGTCAACAAACAGTCCGAG	ATGGAACGGTTCTGGCAAAT	20	20	50.0	45.0	58.1	58.1	0.0	19.4	0.128540	121349	121824	951-951	0.500000	59.0
ndhG_p1	ndhG	1	GAGGGGAATGACATTGGTCC	CAGTCCGATAGCTGCTTCAG	20	20	55.0	55.0	57.9	58.1	5.1	7.8	0.208216	121825	122355	1133-1133	0.500000	58.3
ndhG_p2	ndhG	2	AATCAAATTGCTTTGGGCCG	CAGTCCGATAGCTGCTTCAG	20	20	45.0	55.0	57.9	58.1	0.0	7.8	0.247303	121825	122355	1519-1519	0.500000	58.0
ndhG_p3	ndhG	3	GAGGGGAATGACATTGGTCC	TTTCGGCTCGTGATCAATCC	20	20	55.0	50.0	57.9	58.3	5.1	0.0	0.406605	121825	122355	976-976	0.500000	56.7
ndhG_p4	ndhG	4	TTTGGGCCGTTTACCAATGT	CAGTCCGATAGCTGCTTCAG	20	20	45.0	55.0	58.3	58.1	0.0	7.8	0.443751	121825	122355	1508-1508	0.500000	56.4
ndhG_p5	ndhG	5	AATCAAATTGCTTTGGGCCG	TTTCGGCTCGTGATCAATCC	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.445692	121825	122355	1362-1362	0.500000	56.4
psaC_p1	psaC	1	CTGAAGCAGCTATCGGACTG	TCAGAAAAACCCGTGCTCAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.195185	123156	123401	717-717	0.500000	58.4
psaC_p2	psaC	2	CTGAAGCAGCTATCGGACTG	GAACTTCCCGCAGTGTACAA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.275926	123156	123401	1264-1264	0.500000	57.8
psaC_p3	psaC	3	CTGAAGCAGCTATCGGACTG	CGCCACAGGATACGATTGAT	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.304477	123156	123401	1364-1364	0.500000	57.6
psaC_p4	psaC	4	CTGAAGCAGCTATCGGACTG	AACCCCGTCAGTAAAATGGG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.359313	123156	123401	1024-1024	0.500000	57.1
psaC_p5	psaC	5	CTGAAGCAGCTATCGGACTG	CCCGGCTATTGGCAAAACTA	20	20	55.0	50.0	58.1	58.2	0.0	3.0	0.391941	123156	123401	791-791	0.500000	56.9
psaC-ccsA_p1	psaC-ccsA	1	AAACAACTCGAAGCATGGGT	GAGGCGTGGGGATCATATTG	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.205230	123402	125283	2139-2139	0.500000	58.4
psaC-ccsA_p2	psaC-ccsA	2	AAACAACTCGAAGCATGGGT	GCTTTGCTACTTCGGGTCTT	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.209060	123402	125283	2555-2555	0.500000	58.3
psaC-ccsA_p3	psaC-ccsA	3	AGATGTGAATCCGCTTGTCC	GAGGCGTGGGGATCATATTG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.216325	123402	125283	2197-2197	0.500000	58.3
psaC-ccsA_p4	psaC-ccsA	4	GGCATGAAACAACTCGAAGC	GAGGCGTGGGGATCATATTG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.217383	123402	125283	2145-2145	0.500000	58.3
psaC-ccsA_p5	psaC-ccsA	5	AGATGTGAATCCGCTTGTCC	GCTTTGCTACTTCGGGTCTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.220154	123402	125283	2613-2613	0.500000	58.2
ccsA_p1	ccsA	1	TTATCTCGACGAAATGGGCG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.160012	125284	126255	1830-1830	0.500000	58.7
ccsA_p2	ccsA	2	TTATCTCGACGAAATGGGCG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.161383	125284	126255	1829-1829	0.500000	58.7
ccsA_p3	ccsA	3	GATGGCTTCTCTTGCATTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	57.8	58.0	0.5	0.0	0.213137	125284	126255	1761-1761	0.500000	58.3
ccsA_p4	ccsA	4	GATGGCTTCTCTTGCATTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	57.8	58.0	0.5	0.0	0.214508	125284	126255	1760-1760	0.500000	58.3
ccsA_p5	ccsA	5	CGGGGCCGAGAGAATTATTT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.263948	125284	126255	1537-1537	0.500000	57.9
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAAAGCGCACTTCCGTATCA	20	20	50.0	45.0	58.0	58.2	0.0	11.5	0.204496	126431	127103	836-836	0.500000	58.4
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	AGCGTGTCTACCGATTTCAC	AAAGCGCACTTCCGTATCAA	20	20	50.0	45.0	58.0	58.2	0.0	11.5	0.204496	126431	127103	835-835	0.500000	58.4
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	AAGCGCACTTCCGTATCAAA	20	20	50.0	45.0	58.0	58.2	0.0	11.5	0.204496	126431	127103	834-834	0.500000	58.4
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGCGTGTCTACCGATTTCAC	AGCGCACTTCCGTATCAAAA	20	20	50.0	45.0	58.0	58.2	0.0	11.5	0.204496	126431	127103	833-833	0.500000	58.4
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CAGCGTGTCTACCGATTTCA	AGCGCACTTCCGTATCAAAA	20	20	50.0	45.0	58.0	58.2	0.0	11.5	0.205867	126431	127103	834-834	0.500000	58.4
rpl32-ndhF_p1	rpl32-ndhF	1	TGACCTCAATGCAGGAATGG	GCCCACATACGTCGAAGATT	20	20	50.0	50.0	57.9	58.1	5.9	5.1	0.197349	127251	127880	1280-1280	0.500000	58.4
rpl32-ndhF_p2	rpl32-ndhF	2	TGACCTCAATGCAGGAATGG	AGGAAAAGCCCACATACGTC	20	20	50.0	50.0	57.9	57.9	5.9	0.0	0.242706	127251	127880	1287-1287	0.500000	58.1
rpl32-ndhF_p3	rpl32-ndhF	3	TGACCTCAATGCAGGAATGG	ACATGAAGGGGGAATTGAGC	20	20	50.0	50.0	57.9	57.9	5.9	0.0	0.283254	127251	127880	1901-1901	0.500000	57.7
rpl32-ndhF_p4	rpl32-ndhF	4	TGACCTCAATGCAGGAATGG	AAAAGCCCACATACGTCGAA	20	20	50.0	45.0	57.9	57.8	5.9	0.0	0.306133	127251	127880	1284-1284	0.500000	57.6
rpl32-ndhF_p5	rpl32-ndhF	5	TGACCTCAATGCAGGAATGG	TAAACCGAAATCCCCCACAC	20	20	50.0	50.0	57.9	57.8	5.9	0.0	0.336752	127251	127880	1725-1725	0.500000	57.3
ndhF_p1	ndhF	1	GGTATTTGCCCATTTTGACT	GAGCTCGGGTTATGCAAGAA	20	20	40.0	50.0	54.1	58.0	0.0	0.0	3.921496	127881	130106	2754-2754	0.500000	28.6
ndhF_p2	ndhF	2	GGTATTTGCCCATTTTGACT	AGCTCGGGTTATGCAAGAAG	20	20	40.0	50.0	54.1	58.0	0.0	0.0	3.927158	127881	130106	2753-2753	0.500000	28.6
ndhF_p3	ndhF	3	GGTATTTGCCCATTTTGACT	TGAATTCGGTCGTTGTGGTT	20	20	40.0	45.0	54.1	58.0	0.0	0.0	3.941023	127881	130106	2835-2835	0.500000	28.5
ndhF_p4	ndhF	4	GGTATTTGCCCATTTTGACT	GCAAGAAGGGACCGAAAAGA	20	20	40.0	50.0	54.1	58.1	0.0	0.0	4.005111	127881	130106	2741-2741	0.500000	28.0
ndhF_p5	ndhF	5	GGTATTTGCCCATTTTGACT	GGGCCCTCTTATCTCTTCCT	20	20	40.0	55.0	54.1	57.9	0.0	17.0	4.008073	127881	130106	2779-2779	0.500000	27.9