primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	0.0	0.0	0.944750	1437	1929	547-661	1.000000	84.4
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GCTCTCGCGTCTCTCTAAAA	ACTCCATCCGACTAGTTCCG	20	20	50.0	55.0	57.5	58.6	5.8	0.0	1.155630	1437	1929	496-610	1.000000	82.8
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	ACGGTTTTCAGTGCTGGTTA	ACTAGTTCCGGGTTCGAGTC	20	20	45.0	55.0	57.7	58.8	0.0	0.0	1.163141	1437	1929	537-651	1.000000	82.7
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	ACGGTTTTCAGTGCTGGTTA	GACTAGTTCCGGGTTCGAGT	20	20	45.0	55.0	57.7	58.8	0.0	0.0	1.163141	1437	1929	538-652	1.000000	82.7
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TACAGAAGCGACCCCATAGG	ACTCCATCCGACTAGTTCCG	20	20	55.0	55.0	58.6	58.6	0.0	0.0	1.199430	1437	1929	521-635	1.000000	82.4
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	13671	14868	784-1370	1.000000	90.4
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	CGTTTCCATACCAAGGCTCA	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.240690	13671	14868	819-1405	1.000000	90.1
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ACGTTTCCATACCAAGGCTC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.242165	13671	14868	820-1406	1.000000	90.1
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	13671	14868	816-1402	1.000000	89.5
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	CCCATTGTTAATTCCGGGGT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.285774	13671	14868	1318-1405	0.925000	86.7
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	CCGGTATAGTTCGCACCAAA	CCGGTTCTTTCCATGACTCC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.273870	25840	26079	313-329	1.000000	89.8
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	AAACCGGTATAGTTCGCACC	CCGGTTCTTTCCATGACTCC	20	20	50.0	55.0	58.0	58.3	8.0	0.0	0.275269	25840	26079	316-332	1.000000	89.8
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	GAGAGATGGCTGAGTGGTTG	CCGGTTCTTTCCATGACTCC	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.282288	25840	26079	352-368	1.000000	89.7
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GCTGAGTGGTTGATAGCTCC	CCGGTTCTTTCCATGACTCC	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.314188	25840	26079	344-360	1.000000	89.5
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	GGCTGAGTGGTTGATAGCTC	CCGGTTCTTTCCATGACTCC	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.314188	25840	26079	345-361	1.000000	89.5
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.233981	28511	29783	1221-1461	1.000000	90.1
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.233981	28511	29783	1219-1459	0.950000	88.1
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.233981	28511	29783	1220-1460	0.950000	88.1
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CACAAAAACGAAACGGTCCC	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.256136	28511	29783	1222-1462	0.950000	88.0
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	CCACAAAAACGAAACGGTCC	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.256136	28511	29783	1223-1463	0.950000	88.0
petN-trnC-GCA_p1	petN-trnC-GCA	1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.088172	33339	34331	1010-1093	1.000000	91.3
petN-trnC-GCA_p2	petN-trnC-GCA	2	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.0	58.4	0.0	14.7	0.361535	33339	34331	1028-1111	1.000000	89.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	GCCCAAGCAAGACTCACTAT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.492076	33339	34331	1002-1085	1.000000	88.1
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGCCCAAGCAAGACTCACTA	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	58.6	58.1	0.0	0.0	0.730045	33339	34331	1003-1086	1.000000	86.2
petN-trnC-GCA_p5	petN-trnC-GCA	5	GCCCAAGCAAGACTCACTAT	GGGGGACTGCAAATCCTTTT	20	20	50.0	50.0	57.6	58.4	0.0	14.7	0.765439	33339	34331	1020-1103	1.000000	85.9
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	CCGGATTTGAACTGGGGAAA	GACATTCCCGCATTTTCACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.360154	34403	35286	900-1029	1.000000	89.1
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	CCGGATTTGAACTGGGGAAA	GCCCCGATCAATGAACCTAC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.487797	34403	35286	961-1090	0.975000	87.1
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAAGGATTTGCAGTCCCCC	GACATTCCCGCATTTTCACC	20	20	50.0	50.0	58.4	57.7	14.6	0.0	0.633517	34403	35286	882-1011	1.000000	86.9
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	CCGGATTTGAACTGGGGAAA	TAAGCCCCGATCAATGAACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.423974	34403	35286	964-1093	0.950000	86.6
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	CCGGATTTGAACTGGGGAAA	AGCCCCGATCAATGAACCTA	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.574988	34403	35286	962-1091	0.975000	86.4
trnR-UCU-trnG-UCC_p1	trnR-UCU-trnG-UCC	1	TGCACATAATTTGCGTCCAA	AGCCTTCCAAGCTAACGATG	20	20	40.0	50.0	56.6	58.0	0.0	0.0	1.412752	52319	52759	523-645	1.000000	80.7
trnR-UCU-trnG-UCC_p2	trnR-UCU-trnG-UCC	2	TGCACATAATTTGCGTCCAA	CCTAGCCTTCCAAGCTAACG	20	20	40.0	55.0	56.6	58.1	0.0	0.0	1.441588	52319	52759	526-648	1.000000	80.5
trnR-UCU-trnG-UCC_p3	trnR-UCU-trnG-UCC	3	ATGCACATAATTTGCGTCCA	AGCCTTCCAAGCTAACGATG	20	20	40.0	50.0	56.4	58.0	0.0	0.0	1.641067	52319	52759	524-646	1.000000	78.9
trnR-UCU-trnG-UCC_p4	trnR-UCU-trnG-UCC	4	ATGCACATAATTTGCGTCCA	CCTAGCCTTCCAAGCTAACG	20	20	40.0	55.0	56.4	58.1	0.0	0.0	1.669904	52319	52759	527-649	1.000000	78.6
trnR-UCU-trnG-UCC_p5	trnR-UCU-trnG-UCC	5	TGCACATAATTTGCGTCCAA	CCCTAGCCTTCCAAGCTAAC	20	20	40.0	55.0	56.6	57.7	0.0	0.0	1.716602	52319	52759	527-649	1.000000	78.3
trnQ-UUG-rps16_p1	trnQ-UUG-rps16	1	GAATCAAAAATGGGGCGTGG	GTCTTTCAAGTCGCACGTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013940	55633	56063	557-608	1.000000	91.9
trnQ-UUG-rps16_p2	trnQ-UUG-rps16	2	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	20	20	55.0	50.0	58.1	58.0	16.9	0.0	0.081289	55633	56063	499-550	1.000000	91.3
trnQ-UUG-rps16_p3	trnQ-UUG-rps16	3	GTCGAATCAAAAATGGGGCG	GTCTTTCAAGTCGCACGTTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.097123	55633	56063	560-611	1.000000	91.2
trnQ-UUG-rps16_p4	trnQ-UUG-rps16	4	GAATCAAAAATGGGGCGTGG	ATGTCTTTCAAGTCGCACGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.142887	55633	56063	559-610	1.000000	90.9
trnQ-UUG-rps16_p5	trnQ-UUG-rps16	5	GAATCAAAAATGGGGCGTGG	GCACGTTGCTTTTTACCACA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.205047	55633	56063	545-596	1.000000	90.4
rps16_p1	rps16	1	ACCGGATCCAAACTAAAGGA	AAGTCATCCACACCCTCCTT	20	20	45.0	50.0	56.1	58.3	9.5	0.0	2.111757	56064	57232	1201-1253	0.950000	73.1
rps16_p2	rps16	2	ACCGGATCCAAACTAAAGGA	AGTCATCCACACCCTCCTTT	20	20	45.0	50.0	56.1	58.3	9.5	0.0	2.111757	56064	57232	1200-1252	0.950000	73.1
rps16_p3	rps16	3	ACCGGATCCAAACTAAAGGA	GTCATCCACACCCTCCTTTT	20	20	45.0	50.0	56.1	57.4	9.5	0.0	2.445850	56064	57232	1199-1251	0.975000	71.4
rps16_p4	rps16	4	ACCGGATCCAAACTAAAGGAA	AAGTCATCCACACCCTCCTT	21	20	42.9	50.0	56.9	58.3	9.5	0.0	2.334715	56064	57232	1201-1253	0.950000	71.3
rps16_p5	rps16	5	ACCGGATCCAAACTAAAGGAA	AGTCATCCACACCCTCCTTT	21	20	42.9	50.0	56.9	58.3	9.5	0.0	2.334715	56064	57232	1200-1252	0.950000	71.3
rps16-accD_p1	rps16-accD	1	GGTGCTCAACCTACAGGAAC	AGAATTGAACCACCATTTTTCCA	20	23	55.0	34.8	58.2	57.1	1.3	0.0	4.075765	57233	57939	556-850	0.975000	58.4
rps16-accD_p2	rps16-accD	2	GGTGCTCAACCTACAGGAAC	ACAACATAGAATTGAACCACCA	20	22	55.0	36.4	58.2	55.9	1.3	0.0	4.288790	57233	57939	563-857	0.975000	56.7
rps16-accD_p3	rps16-accD	3	TTTCCTTGAACAGGGTGCTC	AGAATTGAACCACCATTTTTCCA	20	23	50.0	34.8	58.0	57.1	3.1	0.0	3.904192	57233	57939	837-837	0.025000	9.8
rps16-accD_p4	rps16-accD	4	TTTCCTTGAACAGGGTGCTC	ACAACATAGAATTGAACCACCA	20	22	50.0	36.4	58.0	55.9	3.1	0.0	4.117218	57233	57939	844-844	0.025000	8.1
rps16-accD_p5	rps16-accD	5	TTTCCTTGAACAGGGTGCTC	GAATTGAACCACCATTTTTCCA	20	22	50.0	36.4	58.0	55.7	3.1	0.0	4.331246	57233	57939	836-836	0.025000	6.4
accD-psaI_p1	accD-psaI	1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	4.9	26.9	0.027955	59446	59932	492-697	1.000000	91.8
accD-psaI_p2	accD-psaI	2	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	0.0	26.9	0.033461	59446	59932	596-801	1.000000	91.7
accD-psaI_p3	accD-psaI	3	TTGATTCAATCGTACCGCGT	TTTCCAGGAAATCCCCAACG	20	20	45.0	50.0	58.0	58.1	4.9	10.9	0.083080	59446	59932	1058-1263	1.000000	91.3
accD-psaI_p4	accD-psaI	4	AAGGTTCACAAGCAGCTGAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.9	58.0	20.4	26.9	0.084141	59446	59932	534-739	1.000000	91.3
accD-psaI_p5	accD-psaI	5	TTGATTCAATCGTACCGCGT	CCAGGAAATCCCCAACGAAA	20	20	45.0	50.0	58.0	58.1	4.9	0.0	0.083080	59446	59932	1055-1260	0.975000	90.3
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	CGGTCTCAATTGATCCCCTG	TTTACCCTTGGCCATGAACC	20	20	55.0	50.0	58.0	58.1	9.0	19.8	0.109323	66824	67546	1460-1544	0.925000	88.1
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CGGTCTCAATTGATCCCCTG	CTTGGCCATGAACCTCCTTT	20	20	55.0	50.0	58.0	58.1	9.0	13.3	0.112090	66824	67546	1454-1538	0.925000	88.1
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TGATCCCCTGTTACTGCTCA	TCACCGAGTCCAAATACCCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101806	66824	67546	1121-1175	0.450000	68.3
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	GCGTTCTACCGAACTGAACT	TCACCGAGTCCAAATACCCT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.114755	66824	67546	1250-1305	0.450000	68.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CGGTCTCAATTGATCCCCTG	TCACCGAGTCCAAATACCCT	20	20	55.0	50.0	58.0	58.1	9.0	0.0	0.093974	66824	67546	1131-1185	0.375000	65.2
rps8-rpl14_p1	rps8-rpl14	1	TTATGTCAGCGATGGTGTCC	AGAATTTTTGGCGCAATCCC	20	20	50.0	45.0	57.7	57.6	0.0	10.8	0.694541	81745	82088	393-494	1.000000	86.4
rps8-rpl14_p2	rps8-rpl14	2	GTCAGCGATGGTGTCCTTAC	AGAATTTTTGGCGCAATCCC	20	20	55.0	45.0	58.4	57.6	0.0	10.8	0.743436	81745	82088	389-490	1.000000	86.1
rps8-rpl14_p3	rps8-rpl14	3	TATGTCAGCGATGGTGTCCT	AGAATTTTTGGCGCAATCCC	20	20	50.0	45.0	58.5	57.6	0.0	10.8	0.899889	81745	82088	392-493	1.000000	84.8
rps8-rpl14_p4	rps8-rpl14	4	TTATGTCAGCGATGGTGTCC	GAATTTTTGGCGCAATCCCC	20	20	50.0	50.0	57.7	58.6	0.0	10.8	0.940886	81745	82088	392-493	0.925000	81.5
rps8-rpl14_p5	rps8-rpl14	5	GTCAGCGATGGTGTCCTTAC	GAATTTTTGGCGCAATCCCC	20	20	55.0	50.0	58.4	58.6	0.0	10.8	0.989782	81745	82088	388-489	0.925000	81.1
ndhF-rpl32_p1	ndhF-rpl32	1	TGATCCATGAAGATTGATGTGT	GTGCGTTTCTTTGGAACTGC	22	20	36.4	50.0	55.5	58.5	0.0	0.0	5.023265	113422	114162	685-797	1.000000	52.0
ndhF-rpl32_p2	ndhF-rpl32	2	ATGATCCATGAAGATTGATGTGT	GTGCGTTTCTTTGGAACTGC	23	20	34.8	50.0	56.0	58.5	0.0	0.0	5.492938	113422	114162	686-798	1.000000	52.0
ndhF-rpl32_p3	ndhF-rpl32	3	AGGTATGATCCATGAAGATTGATGT	GTGCGTTTCTTTGGAACTGC	25	20	36.0	50.0	57.9	58.5	0.0	0.0	5.610790	113422	114162	690-802	1.000000	52.0
ndhF-rpl32_p4	ndhF-rpl32	4	TGATCCATGAAGATTGATGTGT	AGTGCGTTTCTTTGGAACTG	22	20	36.4	45.0	55.5	56.9	0.0	0.0	5.635523	113422	114162	686-798	1.000000	52.0
ndhF-rpl32_p5	ndhF-rpl32	5	GGTATGATCCATGAAGATTGATGTG	GTGCGTTTCTTTGGAACTGC	25	20	40.0	50.0	57.9	58.5	0.0	0.0	5.665202	113422	114162	689-801	1.000000	52.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACAGGGAATTCAAAAAGTTTT	TCCTAAGAGCAGCGTGTCTA	21	20	28.6	50.0	52.3	57.9	7.9	0.0	6.873103	114310	115365	302-1164	1.000000	52.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACAGGGAATTCAAAAAGTTTT	AGCAGCGTGTCTACCAATTT	21	20	28.6	45.0	52.3	57.8	7.9	0.0	6.933830	114310	115365	299-1157	1.000000	52.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACAGGGAATTCAAAAAGTTTT	CCTAAGAGCAGCGTGTCTAC	21	20	28.6	55.0	52.3	57.8	7.9	0.0	6.945121	114310	115365	303-1163	1.000000	52.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACAGGGAATTCAAAAAGTTTT	CTAAGAGCAGCGTGTCTACC	21	20	28.6	55.0	52.3	57.8	7.9	0.0	6.945121	114310	115365	303-1162	1.000000	52.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACAGGGAATTCAAAAAGTTTTT	TCCTAAGAGCAGCGTGTCTA	22	20	27.3	50.0	53.2	57.9	7.8	0.0	6.955480	114310	115365	302-1164	1.000000	52.0
ndhG-ndhI_p1	ndhG-ndhI	1	CAACCAAGAACCAGTCCCAA	GCGGGAACTGTGTTGAGTAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.160661	120028	120781	862-1180	1.000000	90.7
ndhG-ndhI_p2	ndhG-ndhI	2	CAACCAAGAACCAGTCCCAA	TGGTCAACAAACAGTCCGAG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.117794	120028	120781	1149-1467	0.975000	90.1
ndhG-ndhI_p3	ndhG-ndhI	3	CCCCAACGGTCAAAAGATGA	GCGGGAACTGTGTTGAGTAT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.190475	120028	120781	1017-1335	0.900000	86.5
ndhG-ndhI_p4	ndhG-ndhI	4	CCCCAACGGTCAAAAGATGA	TGGTCAACAAACAGTCCGAG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.147609	120028	120781	1304-1622	0.875000	85.8
ndhG-ndhI_p5	ndhG-ndhI	5	TTGCACCTATCAAAGCAGCT	TGGTCAACAAACAGTCCGAG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.256918	120028	120781	1569-1843	0.850000	83.9
ycf1_p1	ycf1	1	CCCAGGCCTCTCCTATTTCT	GAAGCGTCCGTTGCGATATA	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.424478	129386	129505	1289-1331	0.975000	86.7
ycf1_p2	ycf1	2	TCCCAGGCCTCTCCTATTTC	GAAGCGTCCGTTGCGATATA	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.429767	129386	129505	1290-1332	0.975000	86.7
ycf1_p3	ycf1	3	CGCCTTTGAGCACGTATAGA	GAAGCGTCCGTTGCGATATA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.441381	129386	129505	1044-1062	0.975000	84.8
ycf1_p4	ycf1	4	TCCCCCTCCTCACTTTCTTT	GAAGCGTCCGTTGCGATATA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.371439	129386	129505	1254-1272	0.850000	80.2
ycf1_p5	ycf1	5	TCCCCCTCCTCACTTTCTTT	AAGCGTCCGTTGCGATATAT	20	20	50.0	45.0	57.9	57.6	0.0	0.0	0.567726	129386	129505	1253-1271	0.875000	79.6