primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbK-psbI_p1 psbK-psbI 1 CGCAGTTAGCTTCCGGTAA ATCTAATCCCTACGTCCGGG 19 20 52.6 55.0 57.6 58.1 0.0 15.0 1.508432 7079 7630 682-693 1.000000 71.4 psbK-psbI_p2 psbK-psbI 2 CGCAGTTAGCTTCCGGTAA GGGTTTCGTCCAGGATCATT 19 20 52.6 50.0 57.6 57.6 0.0 0.0 1.849101 7079 7630 664-675 1.000000 68.8 psbK-psbI_p3 psbK-psbI 3 TTTGGCAAGCCGCAGTTAG ATCTAATCCCTACGTCCGGG 19 20 52.6 55.0 59.0 58.1 6.6 15.0 2.123347 7079 7630 692-703 1.000000 66.5 psbK-psbI_p4 psbK-psbI 4 CCGCAGTTAGCTTCCGGTAA ATCTAATCCCTACGTCCGGG 20 20 55.0 55.0 60.1 58.1 0.0 15.0 2.187909 7079 7630 683-694 1.000000 66.0 psbK-psbI_p5 psbK-psbI 5 TTTGGCAAGCCGCAGTTA ATCTAATCCCTACGTCCGGG 18 20 50.0 55.0 57.8 58.1 6.6 15.0 2.245316 7079 7630 692-703 1.000000 65.5 ycf12-trnG-UCC_p1 ycf12-trnG-UCC 1 TCGGGGCCATTAGTAATTGC AGACGAGTCGCACTTTTACC 20 20 50.0 50.0 58.0 57.9 4.4 13.6 0.086080 8609 8776 252-255 1.000000 83.7 ycf12-trnG-UCC_p2 ycf12-trnG-UCC 2 TCGGGGCCATTAGTAATTGC CGAGTCGCACTTTTACCACT 20 20 50.0 50.0 58.0 58.2 4.4 0.0 0.244771 8609 8776 249-252 1.000000 82.5 ycf12-trnG-UCC_p3 ycf12-trnG-UCC 3 TCGGGGCCATTAGTAATTGC GACGAGTCGCACTTTTACCA 20 20 50.0 50.0 58.0 58.2 4.4 13.6 0.249702 8609 8776 251-254 1.000000 82.4 ycf12-trnG-UCC_p4 ycf12-trnG-UCC 4 GCATCGGGGCCATTAGTAAT AGACGAGTCGCACTTTTACC 20 20 50.0 50.0 57.8 57.9 4.4 13.6 0.262113 8609 8776 255-258 1.000000 82.3 ycf12-trnG-UCC_p5 ycf12-trnG-UCC 5 GTGCAACTTGCTATCTTGGC AGACGAGTCGCACTTTTACC 20 20 50.0 50.0 57.7 57.9 14.8 13.6 0.330470 8609 8776 285-288 1.000000 81.3 psbM-petN_p1 psbM-petN 1 GGCCGCGCAGAATAACTAG ACTTGTAGTTCGGGGGAGAA 19 20 57.9 50.0 58.8 58.0 8.7 0.0 1.793539 27965 29171 1257-1277 1.000000 68.6 psbM-petN_p2 psbM-petN 2 GGCCGCGCAGAATAACTAG CCCTCTCACTTGTAGTTCGG 19 20 57.9 55.0 58.8 57.3 8.7 0.0 2.430291 27965 29171 1264-1284 1.000000 63.4 psbM-petN_p3 psbM-petN 3 GGCCGCGCAGAATAACTAG CCTCTCACTTGTAGTTCGGG 19 20 57.9 55.0 58.8 57.3 8.7 0.0 2.430291 27965 29171 1263-1283 1.000000 63.4 psbM-petN_p4 psbM-petN 4 GGCCGCGCAGAATAACTAG CTCTCACTTGTAGTTCGGGG 19 20 57.9 55.0 58.8 57.3 8.7 0.0 2.430291 27965 29171 1262-1282 1.000000 63.4 psbM-petN_p5 psbM-petN 5 GGCCGCGCAGAATAACTAG CTTGTAGTTCGGGGGAGAAG 19 20 57.9 55.0 58.8 57.3 8.7 0.0 2.448527 27965 29171 1256-1276 1.000000 63.3 trnC-GCA-trnG-GCC_p1 trnC-GCA-trnG-GCC 1 CCTACGTCTTACCGCTTGAC TCTTTGCCAAGGAGATGACG 20 20 55.0 50.0 58.1 57.9 0.0 0.0 0.193692 29789 30192 475-478 1.000000 83.1 trnC-GCA-trnG-GCC_p2 trnC-GCA-trnG-GCC 2 GTCCTACGTCTTACCGCTTG TCTTTGCCAAGGAGATGACG 20 20 55.0 50.0 58.1 57.9 0.0 0.0 0.193692 29789 30192 477-480 1.000000 83.1 trnC-GCA-trnG-GCC_p3 trnC-GCA-trnG-GCC 3 AGTCCTACGTCTTACCGCTT TCTTTGCCAAGGAGATGACG 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.267561 29789 30192 478-481 1.000000 82.5 trnC-GCA-trnG-GCC_p4 trnC-GCA-trnG-GCC 4 TCCTACGTCTTACCGCTTGA TCTTTGCCAAGGAGATGACG 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.274051 29789 30192 476-479 1.000000 82.5 trnC-GCA-trnG-GCC_p5 trnC-GCA-trnG-GCC 5 TCGAACTGGGGGATTAAGGA TCTTTGCCAAGGAGATGACG 20 20 50.0 50.0 57.7 57.9 0.0 0.0 0.356532 29789 30192 503-506 1.000000 81.6 trnS-UGA-psbC_p1 trnS-UGA-psbC 1 GTCTTGAAAACTGGCATGGC CGATACCGAACCCGTTCTTT 20 20 50.0 50.0 57.9 58.0 13.3 6.1 0.070691 31170 31456 377-390 1.000000 84.8 trnS-UGA-psbC_p2 trnS-UGA-psbC 2 GTCTTGAAAACTGGCATGGC AAGGAATTGACCGCGATACC 20 20 50.0 50.0 57.9 58.1 13.3 8.2 0.120796 31170 31456 390-403 1.000000 84.3 trnS-UGA-psbC_p3 trnS-UGA-psbC 3 GGGTTCGAATCCCTCTCTCT CGATACCGAACCCGTTCTTT 20 20 55.0 50.0 58.2 58.0 17.2 6.1 0.217358 31170 31456 341-354 1.000000 84.2 trnS-UGA-psbC_p4 trnS-UGA-psbC 4 GTCTTGAAAACTGGCATGGC ACCCGTTCTTTCCATGACAC 20 20 50.0 50.0 57.9 58.1 13.3 0.0 0.178052 31170 31456 368-381 1.000000 84.1 trnS-UGA-psbC_p5 trnS-UGA-psbC 5 GTCTTGAAAACTGGCATGGC GCAGCTGGGTTTGAAAAAGG 20 20 50.0 50.0 57.9 58.1 13.3 17.1 0.200488 31170 31456 406-419 1.000000 83.4 psbD-trnT-GGU_p1 psbD-trnT-GGU 1 CCACGAAGACGAAACGATCT TCTGAAGCCCCCTTAACTCA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.060321 33888 35236 1507-1521 1.000000 82.9 psbD-trnT-GGU_p2 psbD-trnT-GGU 2 CGAAACGATCTCTCCTGAGC TCTGAAGCCCCCTTAACTCA 20 20 55.0 50.0 58.2 58.0 0.0 0.0 0.205003 33888 35236 1498-1512 1.000000 81.8 psbD-trnT-GGU_p3 psbD-trnT-GGU 3 GAAACGATCTCTCCTGAGCC TCTGAAGCCCCCTTAACTCA 20 20 55.0 50.0 57.8 58.0 0.0 0.0 0.256292 33888 35236 1497-1511 1.000000 81.4 psbD-trnT-GGU_p4 psbD-trnT-GGU 4 GATCTCTCCTGAGCCAGTCA TCTGAAGCCCCCTTAACTCA 20 20 55.0 50.0 58.2 58.0 2.2 0.0 0.264753 33888 35236 1492-1506 1.000000 81.3 psbD-trnT-GGU_p5 psbD-trnT-GGU 5 TCTCTCCTGAGCCAGTCATC TCTGAAGCCCCCTTAACTCA 20 20 55.0 50.0 58.2 58.0 2.2 0.0 0.264753 33888 35236 1490-1504 1.000000 81.3 trnM-CAU-atpE_p1 trnM-CAU-atpE 1 AATTACTGGCGGCCTACTTG GCTAGAAGCCTCAAATGTTGC 20 21 50.0 47.6 58.0 57.9 21.5 0.0 1.180791 49604 49760 266-267 1.000000 74.2 trnM-CAU-atpE_p2 trnM-CAU-atpE 2 GCGAGAGTCATTGGTTCGAA GCTAGAAGCCTCAAATGTTGC 20 21 50.0 47.6 58.3 57.9 0.0 0.0 1.432391 49604 49760 216-217 1.000000 73.0 trnM-CAU-atpE_p3 trnM-CAU-atpE 3 TCATACGGCGAGAGTCATTG GCTAGAAGCCTCAAATGTTGC 20 21 50.0 47.6 57.5 57.9 16.6 0.0 1.634423 49604 49760 223-224 1.000000 71.2 trnM-CAU-atpE_p4 trnM-CAU-atpE 4 CATACGGCGAGAGTCATTGG GCTAGAAGCCTCAAATGTTGC 20 21 55.0 47.6 58.5 57.9 16.6 0.0 1.650734 49604 49760 222-223 1.000000 71.1 trnM-CAU-atpE_p5 trnM-CAU-atpE 5 CTTTCATACGGCGAGAGTCA GCTAGAAGCCTCAAATGTTGC 20 21 50.0 47.6 57.4 57.9 16.6 0.0 1.704137 49604 49760 226-227 1.000000 70.6 petA-psbJ_p1 petA-psbJ 1 TTTGAAAAGGTGCAGTTGGC GCCGGTACACTTGTGATAGG 20 20 45.0 55.0 57.7 58.1 0.0 1.9 0.372001 59443 60148 819-832 1.000000 80.6 petA-psbJ_p2 petA-psbJ 2 TTTGAAAAGGTGCAGTTGGC TGTGGTTGGTGGGTACTGTA 20 20 45.0 50.0 57.7 58.2 0.0 0.0 0.504068 59443 60148 839-852 1.000000 79.5 petA-psbJ_p3 petA-psbJ 3 TTTGAAAAGGTGCAGTTGGC CTGTAGCCGGTACACTTGTG 20 20 45.0 55.0 57.7 58.3 0.0 1.9 0.597920 59443 60148 824-837 1.000000 78.8 petA-psbJ_p4 petA-psbJ 4 TTTGAAAAGGTGCAGTTGGC TAGGCGTATTCCCTTACGGA 20 20 45.0 50.0 57.7 57.6 0.0 6.2 0.671240 59443 60148 794-807 1.000000 78.3 petA-psbJ_p5 petA-psbJ 5 TTTGAAAAGGTGCAGTTGGC GGTTCGTTAGGCGTATTCCC 20 20 45.0 55.0 57.7 58.4 0.0 0.0 0.732689 59443 60148 801-814 1.000000 77.8 rpl23-trnI-CAU_p1 rpl23-trnI-CAU 1 TGTTGCAACAAACGAATAGACT TAATTGGCGAATTCACGGGT 22 20 36.4 45.0 56.8 57.9 29.4 10.2 3.271341 81863 82034 262-267 1.000000 59.9 rpl23-trnI-CAU_p2 rpl23-trnI-CAU 2 TGTTGCAACAAACGAATAGACT ATTCACGGGTTCAACTCCTG 22 20 36.4 50.0 56.8 57.8 29.4 0.0 3.339518 81863 82034 252-257 1.000000 59.6 rpl23-trnI-CAU_p3 rpl23-trnI-CAU 3 TGTTGCAACAAACGAATAGACT CGGTTAAAGCACCCAACTCA 22 20 36.4 50.0 56.8 58.4 29.4 0.0 3.554491 81863 82034 282-287 1.000000 57.2 rpl23-trnI-CAU_p4 rpl23-trnI-CAU 4 TGTTGCAACAAACGAATAGACTT TAATTGGCGAATTCACGGGT 23 20 34.8 45.0 57.5 57.9 29.4 10.2 3.609960 81863 82034 262-267 1.000000 57.2 rpl23-trnI-CAU_p5 rpl23-trnI-CAU 5 TGTTGCAACAAACGAATAGACTT ATTCACGGGTTCAACTCCTG 23 20 34.8 50.0 57.5 57.8 29.4 0.0 3.678137 81863 82034 252-257 1.000000 56.9 trnT-UGU-trnR-ACG_p1 trnT-UGU-trnR-ACG 1 ATTGGTGAGCGTAACTCCAC CTCAGGGGATTAGAGCACGT 20 20 50.0 55.0 57.9 58.9 0.0 0.0 0.979295 84058 84800 905-917 1.000000 74.5 trnT-UGU-trnR-ACG_p2 trnT-UGU-trnR-ACG 2 AGTACGGAGGTTCGAATCCT CTCAGGGGATTAGAGCACGT 20 20 50.0 55.0 57.9 58.9 16.1 0.0 1.025372 84058 84800 826-838 1.000000 74.3 trnT-UGU-trnR-ACG_p3 trnT-UGU-trnR-ACG 3 GGATTGGTGAGCGTAACTCC CTCAGGGGATTAGAGCACGT 20 20 55.0 55.0 58.3 58.9 0.0 0.0 1.231382 84058 84800 907-919 1.000000 72.4 trnT-UGU-trnR-ACG_p4 trnT-UGU-trnR-ACG 4 GATTGGTGAGCGTAACTCCA CTCAGGGGATTAGAGCACGT 20 20 50.0 55.0 57.6 58.9 0.0 0.0 1.264617 84058 84800 906-918 1.000000 72.2 trnT-UGU-trnR-ACG_p5 trnT-UGU-trnR-ACG 5 ATTGGTGAGCGTAACTCCAC TCAGGGGATTAGAGCACGTG 20 20 50.0 55.0 57.9 59.2 0.0 12.3 1.270653 84058 84800 904-916 1.000000 72.1 rpl32-trnP-GGG_p1 rpl32-trnP-GGG 1 GCAATAATTGACATCGGCGG GAGGTCACAGGTTCGAATCC 20 20 50.0 55.0 58.0 58.0 7.2 4.4 0.048097 109144 109785 1360-1362 1.000000 83.1 rpl32-trnP-GGG_p2 rpl32-trnP-GGG 2 AGTGGAGCAGGGAAGATTCT GAGGTCACAGGTTCGAATCC 20 20 50.0 55.0 58.0 58.0 0.0 4.4 0.047555 109144 109785 1328-1329 0.500000 61.8 rpl32-trnP-GGG_p3 rpl32-trnP-GGG 3 AGTGGAGCAGGGAAGATTCT GGAGGTCACAGGTTCGAATC 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.047555 109144 109785 1329-1330 0.500000 61.8 rpl32-trnP-GGG_p4 rpl32-trnP-GGG 4 AGTGGAGCAGGGAAGATTCT CAACGTCTATCTGCCCGAAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.040597 109144 109785 1567-1570 0.333333 54.6 rpl32-trnP-GGG_p5 rpl32-trnP-GGG 5 GCAATAATTGACATCGGCGG CAACGTCTATCTGCCCGAAA 20 20 50.0 50.0 58.0 58.0 7.2 0.0 0.041139 109144 109785 1599-1602 0.333333 54.6 trnP-GGG-trnL-UAG_p1 trnP-GGG-trnL-UAG 1 GATTCGAACCTGTGACCTCC CTCTGGCACTGCTTCCTAAG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.032895 109860 110127 363-382 1.000000 88.5 trnP-GGG-trnL-UAG_p2 trnP-GGG-trnL-UAG 2 GATTCGAACCTGTGACCTCC CTGGCACTGCTTCCTAAGAG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.032895 109860 110127 361-380 1.000000 88.5 trnP-GGG-trnL-UAG_p3 trnP-GGG-trnL-UAG 3 GGATTCGAACCTGTGACCTC CTCTGGCACTGCTTCCTAAG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.032895 109860 110127 364-383 1.000000 88.5 trnP-GGG-trnL-UAG_p4 trnP-GGG-trnL-UAG 4 GGATTCGAACCTGTGACCTC CTGGCACTGCTTCCTAAGAG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.032895 109860 110127 362-381 1.000000 88.5 trnP-GGG-trnL-UAG_p5 trnP-GGG-trnL-UAG 5 TCCACTCGGAATAGCAGGAT CTGGCACTGCTTCCTAAGAG 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.109460 109860 110127 378-397 1.000000 87.7 psaC-ndhE_p1 psaC-ndhE 1 AGATTTTCACTGAATGTGCCA GGGTTAGCTACTGCCCTTTC 21 20 38.1 55.0 56.1 58.0 0.0 16.1 2.985651 113733 114077 432-458 1.000000 62.0 psaC-ndhE_p2 psaC-ndhE 2 AGATTTTCACTGAATGTGCCA GCCCTTTCTCTTCAGCGAAA 21 20 38.1 50.0 56.1 58.5 0.0 0.0 3.422076 113733 114077 420-446 1.000000 58.7 psaC-ndhE_p3 psaC-ndhE 3 AGATTTTCACTGAATGTGCCAT GGGTTAGCTACTGCCCTTTC 22 20 36.4 55.0 56.6 58.0 0.0 16.1 3.458762 113733 114077 432-458 1.000000 58.2 psaC-ndhE_p4 psaC-ndhE 4 GATTTTCACTGAATGTGCCA GGGTTAGCTACTGCCCTTTC 20 20 40.0 55.0 54.5 58.0 0.0 16.1 3.567236 113733 114077 431-457 1.000000 57.4 psaC-ndhE_p5 psaC-ndhE 5 AGATTTTCACTGAATGTGCCA TACTGCCCTTTCTCTTCAGC 21 20 38.1 50.0 56.1 57.2 0.0 0.0 3.715997 113733 114077 424-450 1.000000 56.3 ndhE-ndhG_p1 ndhE-ndhG 1 CCAAAAATTAGTCCGTGTTCA CCTTCATCATCTCAAAATAATTCTCCA 21 27 38.1 33.3 54.7 57.9 0.0 0.0 11.427364 114381 114468 150-150 1.000000 45.9 ndhE-ndhG_p2 ndhE-ndhG 2 CCAAAAATTAGTCCGTGTTCAAAC CCTTCATCATCTCAAAATAATTCTCCA 24 27 37.5 33.3 57.5 57.9 0.0 0.0 11.633443 114381 114468 150-150 1.000000 45.9 ndhE-ndhG_p3 ndhE-ndhG 3 CCAAAAATTAGTCCGTGTTCAA CCTTCATCATCTCAAAATAATTCTCCA 22 27 36.4 33.3 55.5 57.9 0.0 0.0 11.641848 114381 114468 150-150 1.000000 45.9 ndhE-ndhG_p4 ndhE-ndhG 4 CCAAAAATTAGTCCGTGTTCA CCTTCATCATCTCAAAATAATTCTCC 21 26 38.1 34.6 54.7 56.5 0.0 0.0 11.846528 114381 114468 150-150 1.000000 45.9 ndhE-ndhG_p5 ndhE-ndhG 5 CCAAAAATTAGTCCGTGTTCAAA CCTTCATCATCTCAAAATAATTCTCCA 23 27 34.8 33.3 56.2 57.9 0.0 0.0 11.923289 114381 114468 150-150 1.000000 45.9 ndhG_p1 ndhG 1 GAAAGGGCAGTAGCTAACCC CTTGCGAAGTATGTGTCCGA 20 20 55.0 50.0 58.0 58.0 16.6 0.0 0.049019 114469 115062 1407-1407 1.000000 82.3 ndhG_p2 ndhG 2 GAAAGGGCAGTAGCTAACCC TTGCGAAGTATGTGTCCGAG 20 20 55.0 50.0 58.0 58.0 16.6 0.0 0.049019 114469 115062 1406-1406 1.000000 82.3 ndhG_p3 ndhG 3 GAAAGGGCAGTAGCTAACCC TGTCCGAGTATGTCCGATCA 20 20 55.0 50.0 58.0 58.0 16.6 0.0 0.086820 114469 115062 1394-1394 1.000000 82.1 ndhG_p4 ndhG 4 GAAAGGGCAGTAGCTAACCC TAGGTAGCAGGTTGGGTTCA 20 20 55.0 50.0 58.0 58.0 16.6 0.0 0.072783 114469 115062 1631-1631 1.000000 81.8 ndhG_p5 ndhG 5 GAAAGGGCAGTAGCTAACCC CCGTAGAAGCTGCGAGATAC 20 20 55.0 55.0 58.0 58.0 16.6 6.5 0.079145 114469 115062 1560-1560 1.000000 81.8 ycf1_p1 ycf1 1 CCAGTTCTCGTCACTTCCAG ATTGCTTCGCCAAAATTCCG 20 20 55.0 45.0 57.9 58.0 0.0 0.0 0.076772 121990 122109 1243-1246 1.000000 81.8 ycf1_p2 ycf1 2 CCAGTTCTCGTCACTTCCAG TGTAGCGGCTAGATCAGACA 20 20 55.0 50.0 57.9 57.9 0.0 10.5 0.124471 121990 122109 1369-1372 1.000000 81.2 ycf1_p3 ycf1 3 CCAGTTCTCGTCACTTCCAG GTGAGGAGTTTTCGCAGGAT 20 20 55.0 50.0 57.9 57.9 0.0 0.0 0.172293 121990 122109 1163-1166 1.000000 81.1 ycf1_p4 ycf1 4 CCAGTTCTCGTCACTTCCAG TCAGACATAGGCCCCATTCA 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.179755 121990 122109 1356-1359 1.000000 80.8 ycf1_p5 ycf1 5 CCAAAGATTGCTCAGGCTGA ATTGCTTCGCCAAAATTCCG 20 20 50.0 45.0 58.2 58.0 17.7 0.0 0.174805 121990 122109 1283-1286 0.666667 66.9 trnR-ACG-trnT-UGU_p1 trnR-ACG-trnT-UGU 1 CTCAGGGGATTAGAGCACGT ATTGGTGAGCGTAACTCCAC 20 20 55.0 50.0 58.9 57.9 0.0 0.0 0.979295 148742 149484 905-917 1.000000 74.5 trnR-ACG-trnT-UGU_p2 trnR-ACG-trnT-UGU 2 CTCAGGGGATTAGAGCACGT AGTACGGAGGTTCGAATCCT 20 20 55.0 50.0 58.9 57.9 0.0 16.1 1.025372 148742 149484 826-838 1.000000 74.3 trnR-ACG-trnT-UGU_p3 trnR-ACG-trnT-UGU 3 CTCAGGGGATTAGAGCACGT GGATTGGTGAGCGTAACTCC 20 20 55.0 55.0 58.9 58.3 0.0 0.0 1.231382 148742 149484 907-919 1.000000 72.4 trnR-ACG-trnT-UGU_p4 trnR-ACG-trnT-UGU 4 CTCAGGGGATTAGAGCACGT GATTGGTGAGCGTAACTCCA 20 20 55.0 50.0 58.9 57.6 0.0 0.0 1.264617 148742 149484 906-918 1.000000 72.2 trnR-ACG-trnT-UGU_p5 trnR-ACG-trnT-UGU 5 TCAGGGGATTAGAGCACGTG ATTGGTGAGCGTAACTCCAC 20 20 55.0 50.0 59.2 57.9 12.3 0.0 1.270653 148742 149484 904-916 1.000000 72.1