primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	GCTTTAGTCCACTCAGCCAT	GTGTCCTATCCATTAGACAATG	20	22	50.0	40.9	57.6	54.1	0.9	0.0	6.325405	9497	11366	1923-1953	1.000000	41.2
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	AGTCCACTCAGCCATCTCTC	GTGTCCTATCCATTAGACAATG	20	22	55.0	40.9	58.5	54.1	0.0	0.0	6.431419	9497	11366	1918-1948	1.000000	41.2
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	ATTAGCAATCCGCCGCTTTA	GTGTCCTATCCATTAGACAATG	20	22	45.0	40.9	58.0	54.1	0.0	0.0	5.953663	9497	11366	1937-1967	1.000000	41.1
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	ATTAGCAATCCGCCGCTTTA	TGTCCTATCCATTAGACAATG	20	21	45.0	38.1	58.0	52.4	0.0	0.0	6.606970	9497	11366	1936-1966	1.000000	41.1
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	ATTAGCAATCCGCCGCTTTA	GTGTCCTATCCATTAGACAAT	20	21	45.0	38.1	58.0	52.4	0.0	0.0	6.611814	9497	11366	1937-1967	1.000000	41.1
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	CTACCAAGACGTTCACCGAG	21	20	47.6	55.0	57.3	58.0	0.0	0.0	1.714249	11439	11626	290-296	1.000000	70.4
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	TTCACCGAGCAAGCAGAAAT	21	20	47.6	45.0	57.3	58.1	0.0	0.0	1.796571	11439	11626	279-285	1.000000	69.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	TCTACCAAGACGTTCACCGA	21	20	47.6	50.0	57.3	58.4	0.0	0.0	2.084736	11439	11626	291-297	1.000000	67.4
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	TTCTACCAAGACGTTCACCG	21	20	47.6	50.0	57.3	57.6	0.0	0.0	2.120022	11439	11626	292-298	1.000000	67.1
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGTTCAAATCCTATTGGACGC	CCGAGCAAGCAGAAATCCTT	21	20	47.6	50.0	57.3	58.5	0.0	0.0	2.235444	11439	11626	275-281	1.000000	66.4
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.336052	33551	33596	189-189	1.000000	77.7
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACCAGCGTAGACATATTGCC	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.337603	33551	33596	190-190	1.000000	77.7
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	AGTGAATTATTGGGCCGAGC	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.416772	33551	33596	219-219	1.000000	77.0
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	GTGAATTATTGGGCCGAGCT	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.416772	33551	33596	218-218	1.000000	77.0
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	TGAATTATTGGGCCGAGCTG	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.417971	33551	33596	217-217	1.000000	77.0
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	GCAGCAGGATTTGAAAAGGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.433848	38100	38365	405-412	1.000000	81.0
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	38100	38365	409-416	1.000000	81.0
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	38100	38365	408-415	1.000000	80.5
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	GCAGCAGGATTTGAAAAGGG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.504093	38100	38365	416-423	1.000000	80.4
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.504584	38100	38365	420-427	1.000000	80.3
psbZ-trnfM-CAU_p1	psbZ-trnfM-CAU	1	CAAGACCGGAGCTATCAACC	CTTGAGGTCACGGGTTCAAA	20	20	55.0	50.0	58.1	58.0	9.5	4.2	0.096882	39021	39821	1425-1433	1.000000	81.4
psbZ-trnfM-CAU_p2	psbZ-trnfM-CAU	2	CAAGACCGGAGCTATCAACC	ACAGATATGGTAGAGGGGCC	20	20	55.0	55.0	58.1	58.0	9.5	0.0	0.090170	39021	39821	1530-1538	1.000000	81.3
psbZ-trnfM-CAU_p3	psbZ-trnfM-CAU	3	GATGCATTGTCCAACATGCC	ACAGATATGGTAGAGGGGCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.100928	39021	39821	1347-1350	1.000000	81.2
psbZ-trnfM-CAU_p4	psbZ-trnfM-CAU	4	CAAGACCGGAGCTATCAACC	TCTTGATTGCCTCCACATCG	20	20	55.0	50.0	58.1	58.0	9.5	0.0	0.087095	39021	39821	2101-2109	1.000000	81.0
psbZ-trnfM-CAU_p5	psbZ-trnfM-CAU	5	GATGCATTGTCCAACATGCC	TCTTGATTGCCTCCACATCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.097853	39021	39821	1918-1921	1.000000	80.9
rps14-psaB_p1	rps14-psaB	1	CTCAACGAGGGAACTTTGCT	CATTGTGCAGGCAAGATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.120347	40366	40496	347-347	1.000000	79.7
rps14-psaB_p2	rps14-psaB	2	CTCAACGAGGGAACTTTGCT	CCATTGTGCAGGCAAGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.120347	40366	40496	348-348	1.000000	79.7
rps14-psaB_p3	rps14-psaB	3	CTCAACGAGGGAACTTTGCT	TCTTGATTGCCTCCACATCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.144185	40366	40496	279-279	1.000000	79.7
rps14-psaB_p4	rps14-psaB	4	CTCAACGAGGGAACTTTGCT	TCCATTGTGCAGGCAAGATT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.188277	40366	40496	349-349	1.000000	79.2
rps14-psaB_p5	rps14-psaB	5	CTCAACGAGGGAACTTTGCT	TTCCATTGTGCAGGCAAGAT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.188277	40366	40496	350-350	1.000000	79.2
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	44980	45701	900-916	1.000000	79.8
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	44980	45701	902-918	1.000000	79.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	44980	45701	829-845	1.000000	78.9
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	44980	45701	833-849	1.000000	78.7
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	44980	45701	896-912	1.000000	78.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAAAAGCCAGCTATCGGAA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043011	49985	50972	1080-1086	1.000000	82.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAAAAGCCAGCTATCGGAA	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.107820	49985	50972	1088-1094	1.000000	82.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49985	50972	1032-1038	1.000000	82.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAGCCAGCTATCGGAATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133544	49985	50972	1077-1083	1.000000	82.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAAGCCAGCTATCGGAATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.198352	49985	50972	1085-1091	1.000000	81.6
accD-psaI_p1	accD-psaI	1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.3	58.0	0.0	26.9	1.728241	62673	63372	840-849	1.000000	68.5
accD-psaI_p2	accD-psaI	2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.3	58.0	0.0	26.9	1.728241	62673	63372	839-848	1.000000	68.5
accD-psaI_p3	accD-psaI	3	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.1	58.0	0.0	26.9	1.960865	62673	63372	840-849	1.000000	66.7
accD-psaI_p4	accD-psaI	4	TCAGTTTCACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.0	58.0	0.0	26.9	1.990387	62673	63372	816-825	1.000000	66.5
accD-psaI_p5	accD-psaI	5	CAGTTTCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.0	58.0	0.0	26.9	1.998036	62673	63372	815-824	1.000000	66.5
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	68305	69594	1347-1358	1.000000	80.9
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	68305	69594	1349-1360	1.000000	80.8
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	68305	69594	1433-1444	1.000000	80.6
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.176991	68305	69594	1435-1446	1.000000	80.6
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.184355	68305	69594	1438-1449	1.000000	80.5
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGGGCTCCTCGAAA	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.234977	72764	73565	900-918	1.000000	80.1
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGGGCTCCTCGAAAG	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.234977	72764	73565	899-917	1.000000	80.1
rpl20-rps12_p3	rpl20-rps12	3	GCTCCTCGAAAGCTTGATGT	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.389396	72764	73565	891-909	1.000000	78.9
rpl20-rps12_p4	rpl20-rps12	4	CTTGAATGGGCTCCTCGAAA	GCCGAGGAACATGTACTAGG	20	20	50.0	55.0	57.9	57.5	0.0	19.0	0.624628	72764	73565	899-917	1.000000	77.0
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGGGCTCCTCGAAAG	GCCGAGGAACATGTACTAGG	20	20	50.0	55.0	57.9	57.5	0.0	19.0	0.624628	72764	73565	898-916	1.000000	77.0
rpl16-rps3_p1	rpl16-rps3	1	GCCCCGCTTCTATTTGTCTA	TGGATATTTGTAGACGAGGA	20	20	50.0	40.0	57.4	52.9	0.0	0.0	5.739320	85354	86654	1428-1429	1.000000	41.5
rpl16-rps3_p2	rpl16-rps3	2	TGTGATCCAAGCAGGTTCAA	TGGATATTTGTAGACGAGGA	20	20	45.0	40.0	57.3	52.9	9.7	0.0	5.779581	85354	86654	1406-1407	1.000000	41.5
rpl16-rps3_p3	rpl16-rps3	3	GCCCCGCTTCTATTTGTCTA	TGGATATTTGTAGACGAGGAA	20	21	50.0	38.1	57.4	53.8	0.0	0.0	5.810385	85354	86654	1428-1429	1.000000	41.5
rpl16-rps3_p4	rpl16-rps3	4	GCCCCGCTTCTATTTGTCTA	TTGGATATTTGTAGACGAGGA	20	21	50.0	38.1	57.4	53.8	0.0	0.0	5.810385	85354	86654	1429-1430	1.000000	41.5
rpl16-rps3_p5	rpl16-rps3	5	TGTGATCCAAGCAGGTTCAA	TGGATATTTGTAGACGAGGAA	20	21	45.0	38.1	57.3	53.8	9.7	0.0	5.850646	85354	86654	1406-1407	1.000000	41.5
trnA-UGC-rrn23_p1	trnA-UGC-rrn23	1	GGTTCGAGTCCGCTTATCTC	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	57.9	58.0	0.0	3.6	0.139468	108414	108585	303-303	1.000000	79.9
trnA-UGC-rrn23_p2	trnA-UGC-rrn23	2	GTTCGAGTCCGCTTATCTCC	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	57.9	58.0	0.0	3.6	0.139468	108414	108585	302-302	1.000000	79.9
trnA-UGC-rrn23_p3	trnA-UGC-rrn23	3	GGTTCGAGTCCGCTTATCTC	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	57.9	57.9	0.0	11.3	0.212163	108414	108585	231-231	1.000000	79.7
trnA-UGC-rrn23_p4	trnA-UGC-rrn23	4	GTTCGAGTCCGCTTATCTCC	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	57.9	57.9	0.0	11.3	0.212163	108414	108585	230-230	1.000000	79.7
trnA-UGC-rrn23_p5	trnA-UGC-rrn23	5	TTCGAGTCCGCTTATCTCCA	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.2	58.0	0.0	3.6	0.257969	108414	108585	301-301	1.000000	79.0
ndhF-rpl32_p1	ndhF-rpl32	1	AGGAATTGGAAGGGGAAGGA	TTTGACATAGAAGTACGTTTTT	20	22	50.0	27.3	57.9	52.0	0.0	0.0	8.037530	116541	117545	1092-1097	1.000000	42.8
ndhF-rpl32_p2	ndhF-rpl32	2	AGGAATTGGAAGGGGAAGGA	TTTTGACATAGAAGTACGTTTT	20	22	50.0	27.3	57.9	52.0	0.0	0.0	8.037530	116541	117545	1093-1098	1.000000	42.8
ndhF-rpl32_p3	ndhF-rpl32	3	AGGAATTGGAAGGGGAAGGA	TTTTTGACATAGAAGTACGTTT	20	22	50.0	27.3	57.9	52.0	0.0	0.0	8.037530	116541	117545	1094-1099	1.000000	42.8
ndhF-rpl32_p4	ndhF-rpl32	4	AGAAGAAGCCCCACTCCTAG	TTTGACATAGAAGTACGTTTTT	20	22	55.0	27.3	58.1	52.0	0.0	0.0	8.089835	116541	117545	1118-1123	1.000000	42.7
ndhF-rpl32_p5	ndhF-rpl32	5	AGAAGAAGCCCCACTCCTAG	TTTTGACATAGAAGTACGTTTT	20	22	55.0	27.3	58.1	52.0	0.0	0.0	8.089835	116541	117545	1119-1124	1.000000	42.7
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	CCACTAACTCTCTACGGAAATCC	TGGCGACTTCGAAGAGTTTC	23	20	47.8	50.0	58.1	58.2	0.0	31.1	3.285915	132199	132318	215-215	1.000000	57.1
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	CGGTTGTTGTATATACTCGAGC	TGGCGACTTCGAAGAGTTTC	22	20	45.5	50.0	56.8	58.2	13.6	31.1	3.426277	132199	132318	193-193	1.000000	56.3
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	CCACTAACTCTCTACGGAAATCC	ATGGCGACTTCGAAGAGTTT	23	20	47.8	45.0	58.1	57.5	0.0	31.1	3.522850	132199	132318	216-216	1.000000	55.2
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	CCACTAACTCTCTACGGAAATCC	AATGGCGACTTCGAAGAGTT	23	20	47.8	45.0	58.1	57.5	0.0	31.1	3.522850	132199	132318	217-217	1.000000	55.1
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	CGGTTGTTGTATATACTCGAGCA	TGGCGACTTCGAAGAGTTTC	23	20	43.5	50.0	58.4	58.2	13.6	31.1	3.616446	132199	132318	193-193	1.000000	54.8
rrn23-trnA-UGC_p1	rrn23-trnA-UGC	1	ATTCGGGAATCTCCGGATCT	GGTTCGAGTCCGCTTATCTC	20	20	50.0	55.0	58.0	57.9	3.6	0.0	0.139468	138920	139091	303-303	1.000000	79.9
rrn23-trnA-UGC_p2	rrn23-trnA-UGC	2	ATTCGGGAATCTCCGGATCT	GTTCGAGTCCGCTTATCTCC	20	20	50.0	55.0	58.0	57.9	3.6	0.0	0.139468	138920	139091	302-302	1.000000	79.9
rrn23-trnA-UGC_p3	rrn23-trnA-UGC	3	GTCTCTGGGTGCCTAGGTAT	GGTTCGAGTCCGCTTATCTC	20	20	55.0	55.0	57.9	57.9	11.3	0.0	0.212163	138920	139091	231-231	1.000000	79.7
rrn23-trnA-UGC_p4	rrn23-trnA-UGC	4	GTCTCTGGGTGCCTAGGTAT	GTTCGAGTCCGCTTATCTCC	20	20	55.0	55.0	57.9	57.9	11.3	0.0	0.212163	138920	139091	230-230	1.000000	79.7
rrn23-trnA-UGC_p5	rrn23-trnA-UGC	5	ATTCGGGAATCTCCGGATCT	TTCGAGTCCGCTTATCTCCA	20	20	50.0	50.0	58.0	58.2	3.6	0.0	0.257969	138920	139091	301-301	1.000000	79.0