primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	AATGGTAAATGGGCGAACGA	GCCGGTAGTAGGTATCTGGT	20	20	45.0	55.0	57.9	58.0	0.0	1.9	0.124289	92	313	537-537	0.125000	44.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATGGTAAATGGGCGAACGA	TGGCCGGTAGTAGGTATCTG	20	20	45.0	55.0	57.9	58.0	0.0	1.9	0.125706	92	313	539-539	0.125000	44.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATGGTAAATGGGCGAACGA	GAGCTTAGTTTCCGTCTGGG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.128367	92	313	977-977	0.125000	44.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATGGTAAATGGGCGAACGA	GGAGCTTAGTTTCCGTCTGG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.128367	92	313	978-978	0.125000	44.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATGGTAAATGGGCGAACGA	GGGAGCTTAGTTTCCGTCTG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.128367	92	313	979-979	0.125000	44.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	ATCCTAATCAAACGAAATTCAA	20	22	50.0	27.3	58.2	52.1	20.3	0.0	8.069933	4165	4883	725-891	1.000000	50.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TCCTAATCAAACGAAATTCAAT	20	22	50.0	27.3	58.2	52.1	20.3	0.0	8.069933	4165	4883	724-890	1.000000	50.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	ATCCTAATCAAACGAAATTCAA	20	22	50.0	27.3	58.2	52.1	20.3	0.0	8.069933	4165	4883	724-890	1.000000	50.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TCCTAATCAAACGAAATTCAAT	20	22	50.0	27.3	58.2	52.1	20.3	0.0	8.069933	4165	4883	723-889	1.000000	50.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TCCTAATCAAACGAAATTCAATT	20	23	50.0	26.1	58.2	53.0	20.3	0.0	8.192086	4165	4883	723-889	1.000000	50.9
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	GACCAAAACCCGTTGCCTTA	AGGCAGAATTAAGACAGATACAGA	20	24	50.0	37.5	58.7	57.2	0.0	0.0	5.477920	7123	7788	553-750	1.000000	52.0
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GACCAAAACCCGTTGCCTTA	GGGCAATTTTCCGAAGAAA	20	19	50.0	42.1	58.7	54.2	0.0	0.0	5.529191	7123	7788	588-794	1.000000	52.0
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	AAAACCCGTTGCCTTACCAC	AGGCAGAATTAAGACAGATACAGA	20	24	50.0	37.5	59.0	57.2	0.0	0.0	5.764071	7123	7788	549-746	1.000000	52.0
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	ACCAAAACCCGTTGCCTTAC	AGGCAGAATTAAGACAGATACAGA	20	24	50.0	37.5	59.0	57.2	0.0	0.0	5.764071	7123	7788	552-749	1.000000	52.0
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	CAAAACCCGTTGCCTTACCA	AGGCAGAATTAAGACAGATACAGA	20	24	50.0	37.5	59.0	57.2	0.0	0.0	5.764853	7123	7788	550-747	1.000000	52.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CTCATTTCCATCCACGAGCA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110548	30345	31491	1230-1351	1.000000	89.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCCTCATTTCCATCCACGAG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.123086	30345	31491	1232-1353	1.000000	89.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CTCATTTCCATCCACGAGCA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.0	58.4	0.0	14.6	0.383911	30345	31491	1212-1333	1.000000	87.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCCTCATTTCCATCCACGAG	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.0	58.4	0.0	14.6	0.396449	30345	31491	1214-1335	1.000000	87.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCCCTCATTTCCATCCACG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.500496	30345	31491	1234-1355	1.000000	86.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	35128	36208	1040-1201	1.000000	90.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	35128	36208	1038-1199	1.000000	90.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.217323	35128	36208	1037-1198	1.000000	89.9
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CTTGCTTTCCTAGTCCACCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.121313	35128	36208	1170-1257	0.375000	62.4
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CTTGCTTTCCTAGTCCACCC	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.218880	35128	36208	1135-1222	0.375000	61.7
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	52423	52740	367-392	1.000000	87.4
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	52423	52740	403-428	1.000000	86.4
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	52423	52740	379-404	1.000000	86.1
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	TTCAAGTCCCTCTATCCCCA	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.0	58.0	0.0	17.4	0.964041	52423	52740	356-381	1.000000	83.7
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	52423	52740	400-425	1.000000	83.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCTTTGTTAATCGGGGCTA	TGAGTCTAAGGGCCTCAACA	20	20	50.0	50.0	58.0	58.0	0.0	6.5	0.071178	55115	56685	963-2115	1.000000	91.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCCTTTGTTAATCGGGGCTA	GGTTGATCTAGGCCAGAAGC	20	20	50.0	55.0	58.0	58.0	0.0	5.0	0.086585	55115	56685	855-2006	1.000000	91.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCTCCGGTAATTGTACAGGC	TGAGTCTAAGGGCCTCAACA	20	20	55.0	50.0	58.1	58.0	0.0	6.5	0.074710	55115	56685	1600-2752	1.000000	90.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CTCCGGTAATTGTACAGGCC	TGAGTCTAAGGGCCTCAACA	20	20	55.0	50.0	58.1	58.0	0.0	6.5	0.074710	55115	56685	2159-2751	0.250000	53.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTCCGGTAATTGTACAGGCC	GGTTGATCTAGGCCAGAAGC	20	20	55.0	55.0	58.1	58.0	0.0	5.0	0.090118	55115	56685	2050-2642	0.250000	53.3
rpl20-rps12_p1	rpl20-rps12	1	ACGAGCTATATATCCCCGTCT	ACATGTACTCGGGTGTAT	21	18	47.6	44.4	57.4	52.1	0.0	0.0	9.596979	74189	75335	1000-1215	1.000000	52.0
rpl20-rps12_p2	rpl20-rps12	2	ACGAGCTATATATCCCCGTC	ACATGTACTCGGGTGTAT	20	18	50.0	44.4	55.8	52.1	0.0	0.0	10.146858	74189	75335	1000-1215	1.000000	52.0
rpl20-rps12_p3	rpl20-rps12	3	CGAGCTATATATCCCCGTCT	ACATGTACTCGGGTGTAT	20	18	50.0	44.4	55.5	52.1	0.0	0.0	10.448015	74189	75335	999-1214	1.000000	52.0
rpl20-rps12_p4	rpl20-rps12	4	ACGAGCTATATATCCCCGTCTA	ACATGTACTCGGGTGTAT	22	18	45.5	44.4	57.2	52.1	0.0	0.0	10.716050	74189	75335	1000-1215	1.000000	52.0
rpl20-rps12_p5	rpl20-rps12	5	TACGAGCTATATATCCCCGTCT	ACATGTACTCGGGTGTAT	22	18	45.5	44.4	57.2	52.1	0.0	0.0	10.716050	74189	75335	1001-1216	1.000000	52.0
Ycf1_p1	Ycf1	1	CCCGGAACTTCTTCGTAGTG	GAAAACCCTTCCTGCAGACA	20	20	55.0	50.0	58.0	58.0	0.7	17.4	0.026574	114326	114857	1186-1302	0.250000	54.0
Ycf1_p2	Ycf1	2	CCGGAACTTCTTCGTAGTGG	GAAAACCCTTCCTGCAGACA	20	20	55.0	50.0	58.0	58.0	0.0	17.4	0.026574	114326	114857	1185-1301	0.250000	54.0
Ycf1_p3	Ycf1	3	AACCCACGACCAATATGAGC	GAAAACCCTTCCTGCAGACA	20	20	50.0	50.0	58.0	58.0	0.0	17.4	0.057130	114326	114857	1226-1342	0.250000	53.8
Ycf1_p4	Ycf1	4	CCCGGAACTTCTTCGTAGTG	AATGGGACGGAAAACCCTTC	20	20	55.0	50.0	58.0	58.1	0.7	0.0	0.088590	114326	114857	1195-1311	0.250000	53.5
Ycf1_p5	Ycf1	5	CCGGAACTTCTTCGTAGTGG	AATGGGACGGAAAACCCTTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.088590	114326	114857	1194-1310	0.250000	53.5
Ycf1-ndhF_p1	Ycf1-ndhF	1	TTCCTTTTCCCAGTTCAGGC	CAGTCTTAACGGGGTGCATT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.183407	114858	115165	944-944	0.250000	48.7
Ycf1-ndhF_p2	Ycf1-ndhF	2	GGCTATGGAAGAAGGAACCG	CAGTCTTAACGGGGTGCATT	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.223857	114858	115165	921-921	0.250000	48.4
Ycf1-ndhF_p3	Ycf1-ndhF	3	CAATTCGGTCGTTTTGGTCG	CAGTCTTAACGGGGTGCATT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233576	114858	115165	1014-1014	0.250000	48.3
Ycf1-ndhF_p4	Ycf1-ndhF	4	GAAGAAGGCAGCAGCAAAAG	CAGTCTTAACGGGGTGCATT	20	20	50.0	50.0	57.9	58.2	2.7	0.0	0.240523	114858	115165	894-894	0.250000	48.3
Ycf1-ndhF_p5	Ycf1-ndhF	5	AGGAACCGAGAACGAGAAGA	CAGTCTTAACGGGGTGCATT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.271794	114858	115165	909-909	0.250000	48.0
ndhF-rpl32_p1	ndhF-rpl32	1	CTTGAGGTCGTGTGAACCAA	AAAGAGGCTCTCCCCATCTT	20	20	50.0	50.0	58.1	58.0	0.0	19.0	0.073535	117389	118080	1775-1783	0.250000	50.0
ndhF-rpl32_p2	ndhF-rpl32	2	AGGAGCCACCCGTATTGATA	AAAGAGGCTCTCCCCATCTT	20	20	50.0	50.0	57.9	58.0	0.0	19.0	0.118749	117389	118080	1296-1304	0.250000	49.7
ndhF-rpl32_p3	ndhF-rpl32	3	TAGGAGCCACCCGTATTGAT	AAAGAGGCTCTCCCCATCTT	20	20	50.0	50.0	57.9	58.0	0.0	19.0	0.118749	117389	118080	1297-1305	0.250000	49.7
ndhF-rpl32_p4	ndhF-rpl32	4	CGTCGGCAAAATAAGAAGGC	AAAGAGGCTCTCCCCATCTT	20	20	50.0	50.0	57.8	58.0	0.0	19.0	0.195699	117389	118080	1357-1365	0.250000	49.1
ndhF-rpl32_p5	ndhF-rpl32	5	AGTGGGATGAAGGGTAGGAG	AAAGAGGCTCTCCCCATCTT	20	20	55.0	50.0	57.8	58.0	0.0	19.0	0.204155	117389	118080	1311-1319	0.250000	49.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACCTACCCCATTTGACCAGT	AACCACTCGGATTGAAGAGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.345405	118240	119227	2013-2019	0.250000	47.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACCTACCCCATTTGACCAGT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.369077	118240	119227	1481-1488	0.250000	47.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACCTACCCCATTTGACCAGT	TAACAGTCCGGAAGTAGCGA	20	20	50.0	50.0	58.2	58.2	0.0	5.2	0.419710	118240	119227	1960-1966	0.250000	47.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACCTACCCCATTTGACCAGT	AGCACTTGACCCGGTTATTG	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.423584	118240	119227	1924-1930	0.250000	47.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACCTACCCCATTTGACCAGT	CTTGACCCGGTTATTGTGCT	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.423584	118240	119227	1920-1926	0.250000	47.3
Ycf1_p1	Ycf1	1	GTCTCCCTTTTCGGTTCCTG	CGACTTGAAGCTCTCGACAA	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.242195	133206	133325	899-1043	1.000000	90.1
Ycf1_p2	Ycf1	2	GGTTCCTGTTCCGATTCCAA	CGACTTGAAGCTCTCGACAA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.246080	133206	133325	887-1031	1.000000	90.0
Ycf1_p3	Ycf1	3	GTCTCCCTTTTCGGTTCCTG	TAAGTGGGCCCTATCGCTTA	20	20	55.0	50.0	58.2	57.9	0.0	25.7	0.281414	133206	133325	643-859	1.000000	89.7
Ycf1_p4	Ycf1	4	TTCCTCGTCTTCCTCGTCTT	TAAGTGGGCCCTATCGCTTA	20	20	50.0	50.0	58.1	57.9	0.0	25.7	0.184109	133206	133325	719-785	0.375000	63.9
Ycf1_p5	Ycf1	5	TTCCTCGTCTTCCTCGTCTT	CGACTTGAAGCTCTCGACAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.144889	133206	133325	903-969	0.375000	63.6
Ycf1-trnN-GUU_p1	Ycf1-trnN-GUU	1	GCCTGAACTGGGAAAAGGAA	CGGGAGAATTGATGACTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033044	135101	135869	853-1157	0.875000	82.5
Ycf1-trnN-GUU_p2	Ycf1-trnN-GUU	2	GCCTGAACTGGGAAAAGGAA	GTAAATCTTCCTCGGCCAGG	20	20	50.0	55.0	58.0	58.0	0.0	13.4	0.049697	135101	135869	970-1274	0.875000	82.1
Ycf1-trnN-GUU_p3	Ycf1-trnN-GUU	3	GCCTGAACTGGGAAAAGGAA	AGAATGATGGGGATAGCGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034651	135101	135869	1134-1438	0.875000	81.8
Ycf1-trnN-GUU_p4	Ycf1-trnN-GUU	4	GCCTGAACTGGGAAAAGGAA	AGATAAACGCGCCTTCACAT	20	20	50.0	45.0	58.0	58.0	0.0	5.7	0.028799	135101	135869	1357-1661	0.750000	76.0
Ycf1-trnN-GUU_p5	Ycf1-trnN-GUU	5	GCCTGAACTGGGAAAAGGAA	ATAAACGCGCCTTCACATCT	20	20	50.0	45.0	58.0	58.0	0.0	5.7	0.028799	135101	135869	1355-1659	0.750000	76.0