primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	ycf3-psaA	1	TCACGAGGCGTTTCGAATAA	TGAGAAATGACCCGGTTTGG	20	20	45.0	50.0	57.6	58.1	15.7	0.0	0.446612	16571	17247	796-817	1.000000	82.8
ycf3-psaA_p2	ycf3-psaA	2	TCACGAGGCGTTTCGAATAA	CCCAGTTCTCGAAAGACGTT	20	20	45.0	50.0	57.6	57.8	15.7	0.0	0.501296	16571	17247	776-797	1.000000	82.5
ycf3-psaA_p3	ycf3-psaA	3	ATCACGAGGCGTTTCGAATA	TGAGAAATGACCCGGTTTGG	20	20	45.0	50.0	57.4	58.1	15.7	0.0	0.667904	16571	17247	797-818	1.000000	81.0
ycf3-psaA_p4	ycf3-psaA	4	ATCACGAGGCGTTTCGAATA	CCCAGTTCTCGAAAGACGTT	20	20	45.0	50.0	57.4	57.8	15.7	0.0	0.722588	16571	17247	777-798	1.000000	80.7
ycf3-psaA_p5	ycf3-psaA	5	TCACGAGGCGTTTCGAATAA	TTTGGCCCAGTTCTCGAAAG	20	20	45.0	50.0	57.6	58.4	15.7	0.0	0.746295	16571	17247	781-802	1.000000	80.5
rps16-trnK-UUU_p1	rps16-trnK-UUU	1	GAACTTCCGTCTAATCAAATGA	AAAGCCGAGTACTCTACCGT	22	20	36.4	50.0	54.2	58.2	0.0	20.3	5.931107	54253	55332	1147-1151	1.000000	46.8
rps16-trnK-UUU_p2	rps16-trnK-UUU	2	GAACTTCCGTCTAATCAAATGA	AAGCCGAGTACTCTACCGTT	22	20	36.4	50.0	54.2	58.2	0.0	20.3	5.931107	54253	55332	1146-1150	1.000000	46.8
rps16-trnK-UUU_p3	rps16-trnK-UUU	3	GAACTTCCGTCTAATCAAATGA	TCTAGCCGCACTTAAAAGCC	22	20	36.4	50.0	54.2	58.3	0.0	0.0	6.028524	54253	55332	1161-1165	1.000000	46.7
rps16-trnK-UUU_p4	rps16-trnK-UUU	4	GAACTTCCGTCTAATCAAATGA	TCAGTCGTGGTCTTCCAAAC	22	20	36.4	50.0	54.2	57.8	0.0	0.0	5.996987	54253	55332	1232-1236	1.000000	46.3
rps16-trnK-UUU_p5	rps16-trnK-UUU	5	GAACTTCCGTCTAATCAAATGA	CAGTCGTGGTCTTCCAAACT	22	20	36.4	50.0	54.2	57.8	0.0	0.0	6.003180	54253	55332	1231-1235	1.000000	46.3
trnK-UUU-accD_p1	trnK-UUU-accD	1	CTAGTTCCGGGTTCGAATCC	CCTCATGTTTAGGAGTTTTTGACA	20	24	55.0	37.5	57.8	57.4	18.0	0.0	4.853979	57881	59569	1746-2631	1.000000	53.0
trnK-UUU-accD_p2	trnK-UUU-accD	2	CTAGTTCCGGGTTCGAATCC	TCCTCATGTTTAGGAGTTTTTGAC	20	24	55.0	37.5	57.8	57.1	18.0	0.0	5.098090	57881	59569	1747-2632	1.000000	51.9
trnK-UUU-accD_p3	trnK-UUU-accD	3	ACTCCATCCGACTAGTTCCG	CCTCATGTTTAGGAGTTTTTGACA	20	24	55.0	37.5	58.6	57.4	0.0	0.0	5.242935	57881	59569	1757-2642	1.000000	51.9
trnK-UUU-accD_p4	trnK-UUU-accD	4	CTAGTTCCGGGTTCGAATCC	CCTCATGTTTAGGAGTTTTTGACAT	20	25	55.0	36.0	57.8	57.8	18.0	0.0	5.439963	57881	59569	1746-2631	1.000000	51.9
trnK-UUU-accD_p5	trnK-UUU-accD	5	CTAGTTCCGGGTTCGAATCC	TCCTCATGTTTAGGAGTTTTTGA	20	23	55.0	34.8	57.8	55.8	18.0	0.0	5.445153	57881	59569	1747-2632	1.000000	51.9
accD_p1	accD	1	GATCTCTTACACAACGCGGT	AGATCGTTTTCTGCAGGCAT	20	20	50.0	45.0	58.0	57.9	12.5	15.1	0.127632	59570	61966	2718-2977	1.000000	85.8
accD_p2	accD	2	TGATCTCTTACACAACGCGG	AGATCGTTTTCTGCAGGCAT	20	20	50.0	45.0	58.0	57.9	12.5	15.1	0.129002	59570	61966	2719-2978	1.000000	85.8
accD_p3	accD	3	GATCTCTTACACAACGCGGT	TTGCAATGGCCGGAAATACT	20	20	50.0	45.0	58.0	58.2	12.5	5.0	0.161824	59570	61966	2531-2785	1.000000	85.6
accD_p4	accD	4	TGATCTCTTACACAACGCGG	TTGCAATGGCCGGAAATACT	20	20	50.0	45.0	58.0	58.2	12.5	5.0	0.163194	59570	61966	2532-2786	1.000000	85.6
accD_p5	accD	5	GATCTCTTACACAACGCGGT	ACCACAGATCGTTTTCTGCA	20	20	50.0	45.0	58.0	57.7	12.5	11.0	0.262376	59570	61966	2982-2982	0.500000	57.9
psaJ-rpl33_p1	psaJ-rpl33	1	CGCTATGGTTCGGGGTTTTA	TGCCCCTTGGTACCTTCTTA	20	20	50.0	50.0	58.0	58.0	0.0	14.4	0.074773	70268	70756	657-666	1.000000	85.7
psaJ-rpl33_p2	psaJ-rpl33	2	TCGGGGTTTTAGCAGGTCTA	TGCCCCTTGGTACCTTCTTA	20	20	50.0	50.0	58.1	58.0	0.0	14.4	0.106392	70268	70756	648-657	1.000000	85.5
psaJ-rpl33_p3	psaJ-rpl33	3	CGCTATGGTTCGGGGTTTTA	ACAATCAATTCGATCCCCCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059685	70268	70756	1027-1034	1.000000	85.3
psaJ-rpl33_p4	psaJ-rpl33	4	TCGGGGTTTTAGCAGGTCTA	ACAATCAATTCGATCCCCCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.091304	70268	70756	1018-1025	1.000000	85.1
psaJ-rpl33_p5	psaJ-rpl33	5	CGCTATGGTTCGGGGTTTTA	GGGACAAAGGCCTACGAAAA	20	20	50.0	50.0	58.0	58.1	0.0	5.2	0.126758	70268	70756	998-1005	1.000000	84.9
rps18_p1	rps18	1	AGAAATAAACTAAACAAACAAACC	TGAATAAAGAATAAGCCTCCCT	24	22	25.0	36.4	52.7	54.6	0.0	0.0	14.760600	71117	71560	479-495	1.000000	47.1
rps18_p2	rps18	2	AGAAATAAACTAAACAAACAAACC	ATGAATAAAGAATAAGCCTCCCT	24	23	25.0	34.8	52.7	55.1	0.0	0.0	15.184289	71117	71560	480-496	1.000000	47.1
rps18_p3	rps18	3	AGAAATAAACTAAACAAACAAACC	TGAATAAAGAATAAGCCTCCC	24	21	25.0	38.1	52.7	53.0	0.0	0.0	15.349892	71117	71560	479-495	1.000000	47.1
rps18_p4	rps18	4	AGAAATAAACTAAACAAACAAACC	AATGAATAAAGAATAAGCCTCCCT	24	24	25.0	33.3	52.7	55.9	0.0	0.0	15.452164	71117	71560	481-497	1.000000	47.1
rps18_p5	rps18	5	AGAAATAAACTAAACAAACAAACC	TCAAATGAATAAAGAATAAGCCTCCC	24	26	25.0	34.6	52.7	57.8	0.0	0.0	15.553029	71117	71560	484-500	1.000000	47.1
psbB-psbT_p1	psbB-psbT	1	GAGCATTCCAAAAACTTGGGG	GAACTTTAGGCGGTTCTCGA	21	20	47.6	50.0	58.0	57.6	4.1	0.0	1.403174	77071	77267	335-336	1.000000	70.7
psbB-psbT_p2	psbB-psbT	2	GGAGCATTCCAAAAACTTGGG	GAACTTTAGGCGGTTCTCGA	21	20	47.6	50.0	58.0	57.6	4.1	0.0	1.403174	77071	77267	336-337	1.000000	70.7
psbB-psbT_p3	psbB-psbT	3	GCTCAAGTAGAGTTTGGAGCA	GAACTTTAGGCGGTTCTCGA	21	20	47.6	50.0	58.0	57.6	0.0	0.0	1.404540	77071	77267	351-352	1.000000	70.6
psbB-psbT_p4	psbB-psbT	4	TGCTCAAGTAGAGTTTGGAGC	GAACTTTAGGCGGTTCTCGA	21	20	47.6	50.0	58.0	57.6	0.0	0.0	1.404540	77071	77267	352-353	1.000000	70.6
psbB-psbT_p5	psbB-psbT	5	TCCAAAAACTTGGGGATCCA	GAACTTTAGGCGGTTCTCGA	20	20	45.0	50.0	56.9	57.6	15.2	0.0	1.442173	77071	77267	329-330	1.000000	70.4
trnM-CAU-ycf2_p1	trnM-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	AGACAAAGCCACATGGAGAC	20	20	50.0	50.0	58.0	57.8	7.6	0.9	0.200329	89664	91970	2244-2384	1.000000	84.2
trnM-CAU-ycf2_p2	trnM-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	GACAAAGCCACATGGAGACT	20	20	50.0	50.0	58.0	57.8	7.6	0.9	0.200329	89664	91970	2243-2383	1.000000	84.2
trnM-CAU-ycf2_p3	trnM-CAU-ycf2	3	AGTTGGGCGCTTTAACCATT	AGACAAAGCCACATGGAGAC	20	20	45.0	50.0	58.4	57.8	7.6	0.9	0.563264	89664	91970	2245-2385	1.000000	81.3
trnM-CAU-ycf2_p4	trnM-CAU-ycf2	4	AGTTGGGCGCTTTAACCATT	GACAAAGCCACATGGAGACT	20	20	45.0	50.0	58.4	57.8	7.6	0.9	0.563264	89664	91970	2244-2384	1.000000	81.3
trnM-CAU-ycf2_p5	trnM-CAU-ycf2	5	TTGGGCGCTTTAACCATTCA	GACAAAGCCACATGGAGACT	20	20	45.0	50.0	58.4	57.8	7.6	0.9	0.569136	89664	91970	2242-2382	1.000000	81.2
rrn5S-trnR-ACG_p1	rrn5S-trnR-ACG	1	TCGTTCATGGTTCGACATCC	GGTACTGACCTTTTTGGCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014876	110490	110728	470-470	0.500000	59.9
rrn5S-trnR-ACG_p2	rrn5S-trnR-ACG	2	CGTTCATGGTTCGACATCCT	GGTACTGACCTTTTTGGCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020439	110490	110728	469-469	0.500000	59.8
rrn5S-trnR-ACG_p3	rrn5S-trnR-ACG	3	TCGTTCATGGTTCGACATCC	TTCAGAATAAAGGGGCTCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042969	110490	110728	987-987	0.500000	59.7
rrn5S-trnR-ACG_p4	rrn5S-trnR-ACG	4	CGTTCATGGTTCGACATCCT	TTCAGAATAAAGGGGCTCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048532	110490	110728	986-986	0.500000	59.6
rrn5S-trnR-ACG_p5	rrn5S-trnR-ACG	5	GGAAAAATAGCTCGAGGCCA	GGTACTGACCTTTTTGGCCA	20	20	50.0	50.0	58.0	58.0	15.6	0.0	0.048681	110490	110728	358-358	0.500000	59.6
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110803	111365	655-673	1.000000	82.3
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.483095	110803	111365	659-677	1.000000	81.4
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	CTCAGTAGCTCAGCGGTAGA	20	20	55.0	55.0	57.8	58.3	18.9	0.0	0.546303	110803	111365	695-713	1.000000	80.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	TCAGTAGCTCAGCGGTAGAG	20	20	55.0	55.0	57.8	58.3	18.9	0.0	0.546303	110803	111365	694-712	1.000000	80.6
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TTGGTCGTAGGTTCGAATCC	20	20	55.0	50.0	57.8	57.3	18.9	4.4	0.876096	110803	111365	656-674	1.000000	78.3
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	TCAGCAAATAAATAGCAGTTCT	20	22	50.0	31.8	58.2	53.8	15.0	0.0	6.346716	111438	113551	2207-2207	0.500000	20.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CTCTACCGCTGAGCTACTGA	TCAGCAAATAAATAGCAGTTCT	20	22	55.0	31.8	58.3	53.8	1.8	0.0	6.520480	111438	113551	2168-2168	0.500000	20.0
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	TCTACCGCTGAGCTACTGAG	TCAGCAAATAAATAGCAGTTCT	20	22	55.0	31.8	58.3	53.8	1.8	0.0	6.520480	111438	113551	2167-2167	0.500000	20.0
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AGGATTCGAACCTACGACCA	TTCAGCAAATAAATAGCAGTTCT	20	23	50.0	30.4	58.2	54.6	15.0	0.0	6.542305	111438	113551	2208-2208	0.500000	20.0
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	TCTACCGCTGAGCTACTGAG	TTCAGCAAATAAATAGCAGTTCT	20	23	55.0	30.4	58.3	54.6	1.8	0.0	6.716069	111438	113551	2168-2168	0.500000	20.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCGGGGTATTGGACATCATT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026574	114471	115216	928-928	0.500000	59.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCGGGGTATTGGACATCATT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027944	114471	115216	929-929	0.500000	59.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGGGGTATTGGACATCATT	TTAGCCACTCGGATTGAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042919	114471	115216	1526-1526	0.500000	59.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGGGGTATTGGACATCATT	AAGCGCGGGTACTAAGATTG	20	20	50.0	50.0	58.0	58.1	0.0	8.8	0.082811	114471	115216	1510-1510	0.500000	59.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGGGGTATTGGACATCATT	ATTGAAGCGCGGGTACTAAG	20	20	50.0	50.0	58.0	58.1	0.0	8.8	0.082811	114471	115216	1514-1514	0.500000	59.3
ndhD_p1	ndhD	1	GGGAGGCCATCAAAAAGCTA	TCTGCTCCAAGGACTGAAGA	20	20	50.0	50.0	57.9	58.0	5.0	0.0	0.159332	117316	118173	1421-1436	1.000000	83.4
ndhD_p2	ndhD	2	GGGGGCCAGAATCAGAAAAA	TCTGCTCCAAGGACTGAAGA	20	20	50.0	50.0	58.1	58.0	4.4	0.0	0.078265	117316	118173	1651-1651	0.500000	59.4
ndhD_p3	ndhD	3	GGGGGCCAGAATCAGAAAAA	TCTCGCTCATCAACCCATTG	20	20	50.0	50.0	58.1	58.0	4.4	0.0	0.091229	117316	118173	1912-1912	0.500000	59.3
ndhD_p4	ndhD	4	GGGGGCCAGAATCAGAAAAA	CTGCTCCAAGGACTGAAGAC	20	20	50.0	55.0	58.1	57.9	4.4	0.0	0.154661	117316	118173	1650-1650	0.500000	58.8
ndhD_p5	ndhD	5	GGGGGCCAGAATCAGAAAAA	GCTCCAAGGACTGAAGACTG	20	20	50.0	55.0	58.1	57.9	4.4	0.0	0.154661	117316	118173	1648-1648	0.500000	58.8
ycf1-rrn5S_p1	ycf1-rrn5S	1	AGCTGTCCCACAAGAAAACC	AGACTGAGAGGTCTTAAGCT	20	20	50.0	45.0	58.3	54.9	0.0	0.0	3.416104	128191	128310	753-753	0.500000	32.7
ycf1-rrn5S_p2	ycf1-rrn5S	2	AACCAGTTCCTGCTGCTATC	AGACTGAGAGGTCTTAAGCT	20	20	50.0	45.0	57.6	54.9	0.0	0.0	3.518463	128191	128310	737-737	0.500000	31.9
ycf1-rrn5S_p3	ycf1-rrn5S	3	ACCAGTTCCTGCTGCTATCT	AGACTGAGAGGTCTTAAGCT	20	20	50.0	45.0	58.4	54.9	0.0	0.0	3.530779	128191	128310	736-736	0.500000	31.8
ycf1-rrn5S_p4	ycf1-rrn5S	4	GTCTCTGTGATTGTGCCAGA	AGACTGAGAGGTCTTAAGCT	20	20	50.0	45.0	57.5	54.9	0.0	0.0	3.570971	128191	128310	874-874	0.500000	31.4
ycf1-rrn5S_p5	ycf1-rrn5S	5	AGTCTCTGTGATTGTGCCAG	AGACTGAGAGGTCTTAAGCT	20	20	50.0	45.0	57.5	54.9	0.0	0.0	3.577788	128191	128310	875-875	0.500000	31.4
ycf2-trnM-CAU_p1	ycf2-trnM-CAU	1	AGACAAAGCCACATGGAGAC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.8	58.0	0.9	7.6	0.200329	146952	149258	2244-2384	1.000000	84.2
ycf2-trnM-CAU_p2	ycf2-trnM-CAU	2	GACAAAGCCACATGGAGACT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.8	58.0	0.9	7.6	0.200329	146952	149258	2243-2383	1.000000	84.2
ycf2-trnM-CAU_p3	ycf2-trnM-CAU	3	AGACAAAGCCACATGGAGAC	AGTTGGGCGCTTTAACCATT	20	20	50.0	45.0	57.8	58.4	0.9	7.6	0.563264	146952	149258	2245-2385	1.000000	81.3
ycf2-trnM-CAU_p4	ycf2-trnM-CAU	4	GACAAAGCCACATGGAGACT	AGTTGGGCGCTTTAACCATT	20	20	50.0	45.0	57.8	58.4	0.9	7.6	0.563264	146952	149258	2244-2384	1.000000	81.3
ycf2-trnM-CAU_p5	ycf2-trnM-CAU	5	GACAAAGCCACATGGAGACT	TTGGGCGCTTTAACCATTCA	20	20	50.0	45.0	57.8	58.4	0.9	7.6	0.569136	146952	149258	2242-2382	1.000000	81.2