primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ssrA-trnR-CCG_p1	ssrA-trnR-CCG	1	GTCGAAACCAAATCAGTCCC	GGTTCGATTCCCTTCATGCT	20	20	50.0	50.0	56.7	58.0	0.0	0.0	1.318844	6233	6286	95-96	1.000000	75.3
ssrA-trnR-CCG_p2	ssrA-trnR-CCG	2	TGTCGAAACCAAATCAGTCCC	GGTTCGATTCCCTTCATGCT	21	20	47.6	50.0	58.5	58.0	0.0	0.0	1.543894	6233	6286	96-97	1.000000	73.5
ssrA-trnR-CCG_p3	ssrA-trnR-CCG	3	GTCGAAACCAAATCAGTCCC	GGGGTTCGATTCCCTTCATG	20	20	50.0	55.0	56.7	58.3	0.0	20.6	1.594384	6233	6286	97-98	1.000000	73.0
ssrA-trnR-CCG_p4	ssrA-trnR-CCG	4	GTCGAAACCAAATCAGTCCC	ATTCTGAAAGTCGGGGGTTC	20	20	50.0	50.0	56.7	57.5	0.0	0.0	1.766397	6233	6286	110-111	1.000000	70.9
ssrA-trnR-CCG_p5	ssrA-trnR-CCG	5	GTCGAAACCAAATCAGTCCC	GATTCTGAAAGTCGGGGGTT	20	20	50.0	50.0	56.7	57.5	0.0	0.0	1.766397	6233	6286	111-112	1.000000	70.9
psbX-rbcS_p1	psbX-rbcS	1	TGCTGTCAAACTCGCGATAA	GCTCGTAAATCATGCGCTTC	20	20	45.0	50.0	57.9	58.0	10.4	9.7	0.083697	7614	8162	801-818	1.000000	89.6
psbX-rbcS_p2	psbX-rbcS	2	TGCTGTCAAACTCGCGATAA	TCGGTGGTGGTACTATTGGT	20	20	45.0	50.0	57.9	58.1	10.4	0.0	0.131740	7614	8162	1309-1326	1.000000	85.6
psbX-rbcS_p3	psbX-rbcS	3	TGCTGTCAAACTCGCGATAA	GAAGCTCGTAAATCATGCGC	20	20	45.0	50.0	57.9	58.0	10.4	0.0	0.083697	7614	8162	804-804	0.500000	59.3
psbX-rbcS_p4	psbX-rbcS	4	TGCTGTCAAACTCGCGATAA	ATCCGCATCCTCGTAACAAC	20	20	45.0	50.0	57.9	58.1	10.4	0.0	0.137184	7614	8162	893-893	0.500000	58.9
psbX-rbcS_p5	psbX-rbcS	5	CGTCTCTTGCAACTTCAGGT	GCTCGTAAATCATGCGCTTC	20	20	50.0	50.0	58.1	58.0	0.0	9.7	0.139500	7614	8162	1160-1160	0.500000	58.9
pbf1-psbH_p1	pbf1-psbH	1	AAGCAGGTCCAAAAGCAGTA	TCAGCATTTAACGGGCGTAA	20	20	45.0	45.0	57.3	57.9	0.0	0.0	0.753167	19132	19215	225-225	1.000000	76.8
pbf1-psbH_p2	pbf1-psbH	2	AAGCAGGTCCAAAAGCAGTA	TTCAGCATTTAACGGGCGTA	20	20	45.0	45.0	57.3	57.9	0.0	0.0	0.753167	19132	19215	226-226	1.000000	76.8
pbf1-psbH_p3	pbf1-psbH	3	GAAGCAGGTCCAAAAGCAGT	TCAGCATTTAACGGGCGTAA	20	20	50.0	45.0	58.7	57.9	0.0	0.0	0.773778	19132	19215	226-226	1.000000	76.6
pbf1-psbH_p4	pbf1-psbH	4	GAAGCAGGTCCAAAAGCAGT	TTCAGCATTTAACGGGCGTA	20	20	50.0	45.0	58.7	57.9	0.0	0.0	0.773778	19132	19215	227-227	1.000000	76.6
pbf1-psbH_p5	pbf1-psbH	5	AGCAGGTCCAAAAGCAGTAT	TCAGCATTTAACGGGCGTAA	20	20	45.0	45.0	57.1	57.9	0.0	0.0	0.983371	19132	19215	224-224	1.000000	75.0
ycf45-ffs_p1	ycf45-ffs	1	CCCGCATTTCAACTTGCAAT	TCCACTGCTCTACCAACTGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.092848	22905	23575	1418-1420	1.000000	87.9
ycf45-ffs_p2	ycf45-ffs	2	CCCGCATTTCAACTTGCAAT	ATCGCATCAATAAACGGCCT	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.115848	22905	23575	1706-1708	1.000000	87.6
ycf45-ffs_p3	ycf45-ffs	3	CCCGCATTTCAACTTGCAAT	AACTGAGCTATCCGGGCTTA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.293644	22905	23575	1404-1406	1.000000	86.3
ycf45-ffs_p4	ycf45-ffs	4	CCCGCATTTCAACTTGCAAT	CCCCCTAGGCACAGTAGAAT	20	20	45.0	55.0	57.9	58.2	0.0	2.1	0.275353	22905	23575	1539-1539	0.500000	57.8
ycf45-ffs_p5	ycf45-ffs	5	CCCGCATTTCAACTTGCAAT	ACTGAGCTATCCGGGCTTAA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.293644	22905	23575	1405-1405	0.500000	57.7
rpoB-rps20_p1	rpoB-rps20	1	GTTTCTCCCTGCCAATTCCA	AAGTTCCATTCTTTGCGGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.081268	38763	39199	1702-1747	1.000000	84.4
rpoB-rps20_p2	rpoB-rps20	2	GTTTCTCCCTGCCAATTCCA	AGTTCCATTCTTTGCGGGTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.081268	38763	39199	1701-1746	1.000000	84.4
rpoB-rps20_p3	rpoB-rps20	3	TGGTTTCTCCCTGCCAATTC	AAGTTCCATTCTTTGCGGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.081268	38763	39199	1704-1749	1.000000	84.4
rpoB-rps20_p4	rpoB-rps20	4	TGGTTTCTCCCTGCCAATTC	AGTTCCATTCTTTGCGGGTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.081268	38763	39199	1703-1748	1.000000	84.4
rpoB-rps20_p5	rpoB-rps20	5	GTTTCTCCCTGCCAATTCCA	TGCGGGTTATTTCGACGATT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.089581	38763	39199	1689-1734	1.000000	84.4
petA-chlI_p1	petA-chlI	1	GCCGTGGACAAGTTTATCCA	GCAAAAGCACATGCAGCATA	20	20	50.0	45.0	58.2	58.0	2.0	7.8	0.184877	44606	45179	1137-1144	1.000000	88.1
petA-chlI_p2	petA-chlI	2	GCCGTGGACAAGTTTATCCA	GGTATGCATGCCGAAATTCG	20	20	50.0	50.0	58.2	58.0	2.0	18.6	0.220482	44606	45179	1401-1408	1.000000	87.3
petA-chlI_p3	petA-chlI	3	GCCGTGGACAAGTTTATCCA	TCGTCATCCTGCTCGATTTG	20	20	50.0	50.0	58.2	58.1	2.0	0.0	0.257364	44606	45179	1483-1490	1.000000	86.7
petA-chlI_p4	petA-chlI	4	GCCGTGGACAAGTTTATCCA	CAATTCGTCATCCTGCTCGA	20	20	50.0	50.0	58.2	58.1	2.0	0.0	0.257364	44606	45179	1487-1494	1.000000	86.6
petA-chlI_p5	petA-chlI	5	GCCGTGGACAAGTTTATCCA	GAGCGAATTGTAGATGCCCA	20	20	50.0	50.0	58.2	58.0	2.0	0.0	0.230367	44606	45179	1269-1269	0.500000	58.2
trnI-CAU-trnS-GCU_p1	trnI-CAU-trnS-GCU	1	GACTCATAAGCCCAAGGTCG	CTTTCGACCACTCAGCCATT	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.245162	49770	49794	95-95	1.000000	81.4
trnI-CAU-trnS-GCU_p2	trnI-CAU-trnS-GCU	2	GACTCATAAGCCCAAGGTCG	TTCGACCACTCAGCCATTTC	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.250264	49770	49794	93-93	1.000000	81.4
trnI-CAU-trnS-GCU_p3	trnI-CAU-trnS-GCU	3	ATAAGCCCAAGGTCGTAGGT	CTTTCGACCACTCAGCCATT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.310999	49770	49794	90-90	1.000000	81.1
trnI-CAU-trnS-GCU_p4	trnI-CAU-trnS-GCU	4	ATAAGCCCAAGGTCGTAGGT	TTCGACCACTCAGCCATTTC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.316101	49770	49794	88-88	1.000000	81.1
trnI-CAU-trnS-GCU_p5	trnI-CAU-trnS-GCU	5	GACTCATAAGCCCAAGGTCG	GACCACTCAGCCATTTCTCC	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.314679	49770	49794	90-90	1.000000	81.0
secG-trnfM-CAU_p1	secG-trnfM-CAU	1	TATGCCCCCATCGTCTAGAG	TACCAGACTGCTCTACTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.115087	55590	55706	536-538	1.000000	87.6
secG-trnfM-CAU_p2	secG-trnfM-CAU	2	CAATCGCTACTGCAGATGGT	TACCAGACTGCTCTACTCCG	20	20	50.0	55.0	58.1	58.0	23.3	0.0	0.094431	55590	55706	811-811	1.000000	86.6
secG-trnfM-CAU_p3	secG-trnfM-CAU	3	CAATCGCTACTGCAGATGGT	CATTGGCCTTCGGGTTATGA	20	20	50.0	50.0	58.1	57.9	23.3	3.3	0.105947	55590	55706	842-842	1.000000	86.4
secG-trnfM-CAU_p4	secG-trnfM-CAU	4	CAATCGCTACTGCAGATGGT	AATCGACCAACTCTGCCATC	20	20	50.0	50.0	58.1	58.0	23.3	0.0	0.084451	55590	55706	1425-1425	1.000000	84.1
secG-trnfM-CAU_p5	secG-trnfM-CAU	5	TATGCCCCCATCGTCTAGAG	AATCGACCAACTCTGCCATC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.105107	55590	55706	1150-1152	1.000000	83.8
psaJ-trnP-UGG_p1	psaJ-trnP-UGG	1	CGATTTTTCCCAGATATGTTAGGA	GGTAGAGTACCTGCTTTGGG	24	20	37.5	55.0	56.6	57.3	0.0	0.0	6.124209	64964	65079	208-229	1.000000	44.8
psaJ-trnP-UGG_p2	psaJ-trnP-UGG	2	CGATTTTTCCCAGATATGTTAGG	GGTAGAGTACCTGCTTTGGG	23	20	39.1	55.0	55.2	57.3	0.0	0.0	6.500849	64964	65079	208-229	1.000000	44.8
psaJ-trnP-UGG_p3	psaJ-trnP-UGG	3	TCGATTTTTCCCAGATATGTTAGGA	GTAGAGTACCTGCTTTGGGAG	25	21	36.0	52.4	57.9	57.5	0.0	0.0	6.660504	64964	65079	208-229	1.000000	44.8
psaJ-trnP-UGG_p4	psaJ-trnP-UGG	4	TCGATTTTTCCCAGATATGTTAGGA	GGTAGAGTACCTGCTTTGGG	25	20	36.0	55.0	57.9	57.3	0.0	0.0	5.825173	64964	65079	209-230	1.000000	44.7
psaJ-trnP-UGG_p5	psaJ-trnP-UGG	5	TCGATTTTTCCCAGATATGTTAGG	GGTAGAGTACCTGCTTTGGG	24	20	37.5	55.0	56.6	57.3	0.0	0.0	6.124209	64964	65079	209-230	1.000000	44.7
trnP-UGG-ycf89_p1	trnP-UGG-ycf89	1	TTACGTGCGGTGAGTACAAG	ATACATCGCGGCACAATTCT	20	20	50.0	45.0	57.9	58.0	0.0	6.1	0.101128	65154	65639	1438-1438	0.500000	59.2
trnP-UGG-ycf89_p2	trnP-UGG-ycf89	2	GTTGGATTGGATGGGTTGGT	ATACATCGCGGCACAATTCT	20	20	50.0	45.0	58.1	58.0	0.0	6.1	0.109250	65154	65639	1467-1467	0.500000	59.1
trnP-UGG-ycf89_p3	trnP-UGG-ycf89	3	TGGTTGGATTGGATGGGTTG	ATACATCGCGGCACAATTCT	20	20	50.0	45.0	58.1	58.0	0.0	6.1	0.110642	65154	65639	1469-1469	0.500000	59.1
trnP-UGG-ycf89_p4	trnP-UGG-ycf89	4	GCGGTGAGTACAAGTGCTAA	ATACATCGCGGCACAATTCT	20	20	50.0	45.0	57.9	58.0	0.0	6.1	0.120988	65154	65639	1432-1432	0.500000	59.0
trnP-UGG-ycf89_p5	trnP-UGG-ycf89	5	TTACGTGCGGTGAGTACAAG	GATCACGTGCCTCGAAATCT	20	20	50.0	50.0	57.9	58.1	0.0	11.7	0.126792	65154	65639	1159-1159	0.500000	59.0
ycf89-rns_p1	ycf89-rns	1	AGAATTGTGCCGCGATGTAT	CATTGCGGAAAATTCCCCAC	20	20	45.0	50.0	58.0	58.0	8.2	6.6	0.055193	66606	67155	1434-1458	1.000000	86.5
ycf89-rns_p2	ycf89-rns	2	AGAATTGTGCCGCGATGTAT	TATTGCTCCGTCAGGCTTTC	20	20	45.0	50.0	58.0	58.0	8.2	0.0	0.070617	66606	67155	1457-1481	1.000000	86.2
ycf89-rns_p3	ycf89-rns	3	AGAATTGTGCCGCGATGTAT	CAACCCATAGGCAGTCTTCC	20	20	45.0	55.0	58.0	58.0	8.2	0.0	0.090564	66606	67155	1486-1510	1.000000	86.0
ycf89-rns_p4	ycf89-rns	4	AGAATTGTGCCGCGATGTAT	ATTGCGGAAAATTCCCCACT	20	20	45.0	45.0	58.0	58.1	8.2	6.6	0.109250	66606	67155	1433-1457	1.000000	86.0
ycf89-rns_p5	ycf89-rns	5	AGAATTGTGCCGCGATGTAT	CTTGCATCCTCCGTCTTACC	20	20	45.0	55.0	58.0	58.1	8.2	0.0	0.105116	66606	67155	1585-1609	1.000000	85.4
ycf46-ccs1_p1	ycf46-ccs1	1	AAAATTGGGCAGTCTCTGGG	ACTTAAAGCGGCAATCATCA	20	20	50.0	40.0	58.1	55.7	0.0	1.4	2.343197	78958	78964	147-147	1.000000	61.8
ycf46-ccs1_p2	ycf46-ccs1	2	AAAATTGGGCAGTCTCTGGG	ACTTAAAGCGGCAATCATCAA	20	21	50.0	38.1	58.1	56.5	0.0	1.4	2.563206	78958	78964	147-147	1.000000	60.0
ycf46-ccs1_p3	ycf46-ccs1	3	AAAATTGGGCAGTCTCTGGG	ACTTAAAGCGGCAATCATCAAT	20	22	50.0	36.4	58.1	57.0	0.0	1.4	3.060411	78958	78964	147-147	1.000000	56.1
ycf46-ccs1_p4	ycf46-ccs1	4	GCGAATTCGTCTTGCTTCTT	ACTTAAAGCGGCAATCATCA	20	20	45.0	40.0	57.1	55.7	27.2	1.4	3.154807	78958	78964	128-128	1.000000	55.4
ycf46-ccs1_p5	ycf46-ccs1	5	GGCGAATTCGTCTTGCTTCT	ACTTAAAGCGGCAATCATCA	20	20	50.0	40.0	58.9	55.7	27.2	1.4	3.192562	78958	78964	129-129	1.000000	55.1
thiS-psb28_p1	thiS-psb28	1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028923	85038	85536	1299-1340	1.000000	88.2
thiS-psb28_p2	thiS-psb28	2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028923	85038	85536	1298-1339	1.000000	88.2
thiS-psb28_p3	thiS-psb28	3	TCACAACCCACTGCCTTAAC	CCTGCCACCCCAGTTTTTAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087149	85038	85536	1270-1311	1.000000	87.9
thiS-psb28_p4	thiS-psb28	4	GACCAGGCCTAGCAATCTTC	CTTTGACTCCGCCATTCGTA	20	20	55.0	50.0	58.0	58.0	4.0	0.0	0.044166	85038	85536	1502-1502	0.500000	59.6
thiS-psb28_p5	thiS-psb28	5	GACCAGGCCTAGCAATCTTC	TTTGACTCCGCCATTCGTAG	20	20	55.0	50.0	58.0	58.0	4.0	0.0	0.044166	85038	85536	1501-1501	0.500000	59.6
rps19-ycf88_p1	rps19-ycf88	1	TCACATATTAAAGCCGACAA	ATGTGGAAACTTTTTGGAAAA	20	21	35.0	28.6	52.6	52.6	0.0	0.0	11.829509	92891	92903	95-96	1.000000	43.4
rps19-ycf88_p2	rps19-ycf88	2	TCACATATTAAAGCCGACAA	TGTGGAAACTTTTTGGAAAAT	20	21	35.0	28.6	52.6	52.6	0.0	0.0	11.829509	92891	92903	94-95	1.000000	43.4
rps19-ycf88_p3	rps19-ycf88	3	TCACATATTAAAGCCGACAA	AATGTGGAAACTTTTTGGAAA	20	21	35.0	28.6	52.6	52.6	0.0	0.0	11.829509	92891	92903	96-97	1.000000	43.3
rps19-ycf88_p4	rps19-ycf88	4	ACATTTAAATCACATATTAAAGCCGAC	ATGTTAACTCTAATCGTGCCA	27	21	29.6	38.1	57.3	55.0	0.0	7.2	11.687850	92891	92903	80-80	0.500000	20.0
rps19-ycf88_p5	rps19-ycf88	5	ACATTTAAATCACATATTAAAGCCGA	ATGTTAACTCTAATCGTGCCA	26	21	26.9	38.1	56.1	55.0	0.0	7.2	11.861645	92891	92903	80-80	0.500000	20.0
ccs1-ycf46_p1	ccs1-ycf46	1	ACTTAAAGCGGCAATCATCA	AAAATTGGGCAGTCTCTGGG	20	20	40.0	50.0	55.7	58.1	1.4	0.0	2.343197	115272	115278	147-147	1.000000	61.8
ccs1-ycf46_p2	ccs1-ycf46	2	ACTTAAAGCGGCAATCATCAA	AAAATTGGGCAGTCTCTGGG	21	20	38.1	50.0	56.5	58.1	1.4	0.0	2.563206	115272	115278	147-147	1.000000	60.0
ccs1-ycf46_p3	ccs1-ycf46	3	ACTTAAAGCGGCAATCATCAAT	AAAATTGGGCAGTCTCTGGG	22	20	36.4	50.0	57.0	58.1	1.4	0.0	3.060411	115272	115278	147-147	1.000000	56.1
ccs1-ycf46_p4	ccs1-ycf46	4	ACTTAAAGCGGCAATCATCA	GCGAATTCGTCTTGCTTCTT	20	20	40.0	45.0	55.7	57.1	1.4	27.2	3.154807	115272	115278	128-128	1.000000	55.4
ccs1-ycf46_p5	ccs1-ycf46	5	ACTTAAAGCGGCAATCATCA	GGCGAATTCGTCTTGCTTCT	20	20	40.0	50.0	55.7	58.9	1.4	27.2	3.192562	115272	115278	129-129	1.000000	55.1
rns-ycf89_p1	rns-ycf89	1	CATTGCGGAAAATTCCCCAC	AGAATTGTGCCGCGATGTAT	20	20	50.0	45.0	58.0	58.0	6.6	8.2	0.055193	127081	127630	1434-1458	1.000000	86.5
rns-ycf89_p2	rns-ycf89	2	TATTGCTCCGTCAGGCTTTC	AGAATTGTGCCGCGATGTAT	20	20	50.0	45.0	58.0	58.0	0.0	8.2	0.070617	127081	127630	1457-1481	1.000000	86.2
rns-ycf89_p3	rns-ycf89	3	CAACCCATAGGCAGTCTTCC	AGAATTGTGCCGCGATGTAT	20	20	55.0	45.0	58.0	58.0	0.0	8.2	0.090564	127081	127630	1486-1510	1.000000	86.0
rns-ycf89_p4	rns-ycf89	4	ATTGCGGAAAATTCCCCACT	AGAATTGTGCCGCGATGTAT	20	20	45.0	45.0	58.1	58.0	6.6	8.2	0.109250	127081	127630	1433-1457	1.000000	86.0
rns-ycf89_p5	rns-ycf89	5	CTTGCATCCTCCGTCTTACC	AGAATTGTGCCGCGATGTAT	20	20	55.0	45.0	58.1	58.0	0.0	8.2	0.105116	127081	127630	1585-1609	1.000000	85.4
ycf89-trnP-UGG_p1	ycf89-trnP-UGG	1	ATACATCGCGGCACAATTCT	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	58.0	57.9	6.1	0.0	0.155866	128597	129082	974-974	0.500000	58.8
ycf89-trnP-UGG_p2	ycf89-trnP-UGG	2	GATCACGTGCCTCGAAATCT	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	58.1	57.9	11.7	0.0	0.181530	128597	129082	695-695	0.500000	58.5
ycf89-trnP-UGG_p3	ycf89-trnP-UGG	3	AATTGATCACGTGCCTCGAA	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	57.9	57.9	11.7	0.0	0.202731	128597	129082	699-699	0.500000	58.4
ycf89-trnP-UGG_p4	ycf89-trnP-UGG	4	ATTGATCACGTGCCTCGAAA	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	57.9	57.9	11.7	0.0	0.202731	128597	129082	698-698	0.500000	58.4
ycf89-trnP-UGG_p5	ycf89-trnP-UGG	5	TTGATCACGTGCCTCGAAAT	AGGATTTGAACCTACGGCAC	20	20	45.0	50.0	57.9	57.9	11.7	0.0	0.202731	128597	129082	697-697	0.500000	58.4