primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ccsA	1	GGTTTTGGACGGCTTTTACG	ACAGGTTTACCTTTGCCGTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.102224	22358	23317	2064-2064	0.500000	59.2
ccsA_p2	ccsA	2	AGGTGGTGGGGTTCGTAATA	ACAGGTTTACCTTTGCCGTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.106058	22358	23317	2044-2044	0.500000	59.2
ccsA_p3	ccsA	3	GGTTTTGGACGGCTTTTACG	GAATTTTCCCGACCACAGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.135077	22358	23317	2078-2078	0.500000	58.9
ccsA_p4	ccsA	4	AGGTGGTGGGGTTCGTAATA	GAATTTTCCCGACCACAGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138912	22358	23317	2058-2058	0.500000	58.9
ccsA_p5	ccsA	5	GCTTGGACAGAAGATGGGTT	ACAGGTTTACCTTTGCCGTT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.268086	22358	23317	2325-2325	0.500000	57.9
ftsW_p1	ftsW	1	AACCGGCTGTAATCCATCTA	AGGACTTGAACCTACGACCA	20	20	45.0	50.0	56.0	58.0	0.0	0.0	1.990296	23857	25047	2130-2130	0.500000	44.1
ftsW_p2	ftsW	2	ACCGGCTGTAATCCATCTAA	AGGACTTGAACCTACGACCA	20	20	45.0	50.0	56.0	58.0	0.0	0.0	1.990296	23857	25047	2129-2129	0.500000	44.1
ftsW_p3	ftsW	3	TAACCGGCTGTAATCCATCT	AGGACTTGAACCTACGACCA	20	20	45.0	50.0	56.0	58.0	0.0	0.0	1.990296	23857	25047	2131-2131	0.500000	44.1
ftsW_p4	ftsW	4	AACCGGCTGTAATCCATCTA	CTCCCCAGGTAGGACTTGAA	20	20	45.0	55.0	56.0	58.0	0.0	0.0	2.032304	23857	25047	2140-2140	0.500000	43.7
ftsW_p5	ftsW	5	ACCGGCTGTAATCCATCTAA	CTCCCCAGGTAGGACTTGAA	20	20	45.0	55.0	56.0	58.0	0.0	0.0	2.032304	23857	25047	2139-2139	0.500000	43.7
psaC_p1	psaC	1	AGCCAACGCAATTGTATTGC	TTCAATCAAATCGGCACCCA	20	20	45.0	45.0	58.0	58.1	13.0	0.0	0.088579	25580	25825	1264-1264	0.500000	59.3
psaC_p2	psaC	2	GCCAACGCAATTGTATTGCT	TTCAATCAAATCGGCACCCA	20	20	45.0	45.0	58.0	58.1	18.8	0.0	0.088579	25580	25825	1263-1263	0.500000	59.3
psaC_p3	psaC	3	TGGTCGTAGGTTCAAGTCCT	TTCAATCAAATCGGCACCCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.088865	25580	25825	946-946	0.500000	59.3
psaC_p4	psaC	4	TTCAAGTCCTACCTGGGGAG	TTCAATCAAATCGGCACCCA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.130873	25580	25825	936-936	0.500000	59.0
psaC_p5	psaC	5	GCCAACGCAATTGTATTGCT	TCAATCAAATCGGCACCCAT	20	20	45.0	45.0	58.0	57.9	18.8	0.0	0.147440	25580	25825	1262-1262	0.500000	58.8
mntB_p1	mntB	1	TGGGTGCCGATTTGATTGAA	CGTGAACTTTCTGGTGGACA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.135390	26814	27698	1842-1842	0.500000	58.9
mntB_p2	mntB	2	TGGGTGCCGATTTGATTGAA	GTTATGTTCCACAACGTGCG	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.138287	26814	27698	2021-2021	0.500000	58.9
mntB_p3	mntB	3	TGGGTGCCGATTTGATTGAA	GGACCAAATGGAGCAGGAAA	20	20	45.0	50.0	58.1	58.1	0.0	6.4	0.160139	26814	27698	2139-2139	0.500000	58.7
mntB_p4	mntB	4	TGGGTGCCGATTTGATTGAA	GCTCGTTTACTTGCTCAGGA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.163780	26814	27698	1803-1803	0.500000	58.7
mntB_p5	mntB	5	TGGGTGCCGATTTGATTGAA	AGCTCGTTTACTTGCTCAGG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.169549	26814	27698	1804-1804	0.500000	58.6
mntA_p1	mntA	1	TGCAATTGCTAAACCCGGTA	GGATGAGAATTATCGTTGGCC	20	21	45.0	47.6	57.8	56.8	29.4	0.0	2.379602	27688	28422	1047-1047	0.500000	41.0
mntA_p2	mntA	2	TGCAATTGCTAAACCCGGTA	TGAGAATTATCGTTGGCCTTCA	20	22	45.0	40.9	57.8	57.8	29.4	3.3	2.416907	27688	28422	1044-1044	0.500000	40.7
mntA_p3	mntA	3	TGCAATTGCTAAACCCGGTA	AGGATGAGAATTATCGTTGGCC	20	22	45.0	45.5	57.8	58.3	29.4	0.0	2.457261	27688	28422	1048-1048	0.500000	40.3
mntA_p4	mntA	4	TGCAATTGCTAAACCCGGTA	GGATGAGAATTATCGTTGGCCT	20	22	45.0	45.5	57.8	58.3	29.4	3.3	2.457261	27688	28422	1047-1047	0.500000	40.3
mntA_p5	mntA	5	TAAACCAGTTGCACCAGGTG	GGATGAGAATTATCGTTGGCC	20	21	50.0	47.6	58.3	56.8	16.2	0.0	2.491326	27688	28422	1515-1515	0.500000	40.1
ftsQ_p1	ftsQ	1	TGTCCACCAGAAAGTTCACG	GCATCTGCGCGTATTGAATC	20	20	50.0	50.0	58.1	58.1	0.0	8.4	0.109311	28481	29335	1885-1885	0.500000	59.1
ftsQ_p2	ftsQ	2	TTTCCTGCTCCATTTGGTCC	GCATCTGCGCGTATTGAATC	20	20	50.0	50.0	58.1	58.1	0.0	8.4	0.134059	28481	29335	1588-1588	0.500000	58.9
ftsQ_p3	ftsQ	3	TCCTGAGCAAGTAAACGAGC	GCATCTGCGCGTATTGAATC	20	20	50.0	50.0	57.9	58.1	0.0	8.4	0.137701	28481	29335	1924-1924	0.500000	58.9
ftsQ_p4	ftsQ	4	CCTGAGCAAGTAAACGAGCT	GCATCTGCGCGTATTGAATC	20	20	50.0	50.0	57.9	58.1	0.0	8.4	0.143469	28481	29335	1923-1923	0.500000	58.9
ftsQ_p5	ftsQ	5	CCTGCTCCATTTGGTCCAAT	GCATCTGCGCGTATTGAATC	20	20	50.0	50.0	58.1	58.1	4.8	8.4	0.200786	28481	29335	1585-1585	0.500000	58.4
apcF_p1	apcF	1	CCAAAGTGGACTACGAATAAACC	ACTCCTACAGGAAGCCGAAT	23	20	43.5	50.0	57.5	58.1	0.0	0.0	3.673735	29460	29969	972-972	0.500000	30.6
apcF_p2	apcF	2	CCAAAGTGGACTACGAATAAACC	CAAACTCCTACAGGAAGCCG	23	20	43.5	55.0	57.5	58.3	0.0	0.0	3.814245	29460	29969	975-975	0.500000	29.5
apcF_p3	apcF	3	CCAAAGTGGACTACGAATAAACC	AAACTCCTACAGGAAGCCGA	23	20	43.5	50.0	57.5	58.4	0.0	0.0	3.900182	29460	29969	974-974	0.500000	28.8
apcF_p4	apcF	4	CCAAAGTGGACTACGAATAAACC	AACTCCTACAGGAAGCCGAA	23	20	43.5	50.0	57.5	58.4	0.0	0.0	3.900182	29460	29969	973-973	0.500000	28.8
apcF_p5	apcF	5	TCGGTTCAATTTCAAATTGGAGT	ACTCCTACAGGAAGCCGAAT	23	20	34.8	50.0	57.2	58.1	20.4	0.0	3.915530	29460	29969	1009-1009	0.500000	28.7
cpcB_p1	cpcB	1	GTGGTCCAGCATCCCATTTA	GATGCATACTGAGGGCCAAA	20	20	50.0	50.0	57.6	57.9	0.0	6.2	0.492510	31240	31758	1326-1326	0.500000	56.1
cpcB_p2	cpcB	2	GTGGTCCAGCATCCCATTTA	TAGATGCATACTGAGGGCCA	20	20	50.0	50.0	57.6	57.9	0.0	6.2	0.537012	31240	31758	1328-1328	0.500000	55.7
cpcB_p3	cpcB	3	GTGGTCCAGCATCCCATTTA	CAGCTTGGGGAAAGATCGAA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.549233	31240	31758	1482-1482	0.500000	55.6
cpcB_p4	cpcB	4	GTGGTCCAGCATCCCATTTA	AGCTTGGGGAAAGATCGAAC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.550715	31240	31758	1481-1481	0.500000	55.6
cpcB_p5	cpcB	5	GTGGTCCAGCATCCCATTTA	ACGCACGGTTCATTTCTTCT	20	20	50.0	45.0	57.6	58.1	0.0	0.0	0.556419	31240	31758	1463-1463	0.500000	55.5
crtE_p1	crtE	1	TTTGGCCCTCAGTATGCATC	GCAAGCAGCTCGCATTTTTA	20	20	50.0	45.0	57.9	58.0	0.2	0.0	0.075884	32445	33356	1422-1422	0.500000	59.4
crtE_p2	crtE	2	TTTGGCCCTCAGTATGCATC	TGTGCAATCGGGATTAGGTG	20	20	50.0	50.0	57.9	58.0	0.2	0.0	0.094095	32445	33356	1569-1569	0.500000	59.2
crtE_p3	crtE	3	TGGCCCTCAGTATGCATCTA	GCAAGCAGCTCGCATTTTTA	20	20	50.0	45.0	57.9	58.0	0.2	0.0	0.120386	32445	33356	1420-1420	0.500000	59.0
crtE_p4	crtE	4	TTCGATCTTTCCCCAAGCTG	GCAAGCAGCTCGCATTTTTA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.132607	32445	33356	1266-1266	0.500000	58.9
crtE_p5	crtE	5	GTTCGATCTTTCCCCAAGCT	GCAAGCAGCTCGCATTTTTA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.134089	32445	33356	1267-1267	0.500000	58.9
rbcR_p1	rbcR	1	TTAGTAGGTTTTCCGCGTCG	GACGCACGGGTAGTAAAGTT	20	20	50.0	50.0	58.0	57.9	4.9	0.0	0.089008	33395	34369	1449-1449	0.500000	59.3
rbcR_p2	rbcR	2	TTAGTAGGTTTTCCGCGTCG	GAGACGCACGGGTAGTAAAG	20	20	50.0	55.0	58.0	58.1	4.9	0.0	0.113297	33395	34369	1451-1451	0.500000	59.1
rbcR_p3	rbcR	3	AAGCCATTTCCATGCTTCCA	GACGCACGGGTAGTAAAGTT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.128884	33395	34369	1346-1346	0.500000	59.0
rbcR_p4	rbcR	4	CCATGCTTCCACCAGCTAAT	GACGCACGGGTAGTAAAGTT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.133232	33395	34369	1337-1337	0.500000	58.9
rbcR_p5	rbcR	5	AAGCCATTTCCATGCTTCCA	GAGACGCACGGGTAGTAAAG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.153173	33395	34369	1348-1348	0.500000	58.8
ycf21_p1	ycf21	1	AACTTTACTACCCGTGCGTC	GGAGAAGCAAATCCACCACA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.160562	34714	35322	1475-1475	0.500000	58.7
ycf21_p2	ycf21	2	CTTTACTACCCGTGCGTCTC	GGAGAAGCAAATCCACCACA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.184851	34714	35322	1473-1473	0.500000	58.5
ycf21_p3	ycf21	3	AACTTTACTACCCGTGCGTC	ATGGGTTCTCGCCTTTTCTC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.185604	34714	35322	1581-1581	0.500000	58.5
ycf21_p4	ycf21	4	AACTTTACTACCCGTGCGTC	GATGGGTTCTCGCCTTTTCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.185604	34714	35322	1582-1582	0.500000	58.5
ycf21_p5	ycf21	5	GCTCGTCTTCCACTACGATC	GGAGAAGCAAATCCACCACA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.198478	34714	35322	1750-1750	0.500000	58.4
chlL_p1	chlL	1	TAACGACCCCAAAAAGGACC	TCGGGGTTCAGCAAAAATCA	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.286841	35531	36382	1799-1799	0.500000	57.7
chlL_p2	chlL	2	TAACGACCCCAAAAAGGACC	GCGTAGTCCAAACCATTTGC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.297946	35531	36382	2032-2032	0.500000	57.6
chlL_p3	chlL	3	CGACCCCAAAAAGGACCTTT	TCGGGGTTCAGCAAAAATCA	20	20	50.0	45.0	58.3	58.0	14.0	0.0	0.311977	35531	36382	1796-1796	0.500000	57.5
chlL_p4	chlL	4	CGACCCCAAAAAGGACCTTT	GCGTAGTCCAAACCATTTGC	20	20	50.0	50.0	58.3	58.0	14.0	0.0	0.323082	35531	36382	2029-2029	0.500000	57.4
chlL_p5	chlL	5	TAACGACCCCAAAAAGGACC	GTCCAAACCATTTGCACGAG	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.325611	35531	36382	2027-2027	0.500000	57.4
chlN_p1	chlN	1	TGTGGTGGATTTGCTTCTCC	ACGCCACTTTTTAGCTCCAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.114641	36427	37803	2082-2082	0.500000	59.1
chlN_p2	chlN	2	TGTGGTGGATTTGCTTCTCC	GGAGAAGCTTTTTCACGTGC	20	20	50.0	50.0	58.1	58.0	0.0	11.7	0.128241	36427	37803	2553-2553	0.500000	59.0
chlN_p3	chlN	3	AGAAAAGGCGAGAACCCATC	ACGCCACTTTTTAGCTCCAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.139683	36427	37803	1975-1975	0.500000	58.9
chlN_p4	chlN	4	GAGAAAAGGCGAGAACCCAT	ACGCCACTTTTTAGCTCCAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.139683	36427	37803	1976-1976	0.500000	58.9
chlN_p5	chlN	5	TGTGGTGGATTTGCTTCTCC	AAACGTGGTCATCGACAACA	20	20	50.0	45.0	58.1	58.1	0.0	2.8	0.147623	36427	37803	2018-2018	0.500000	58.8
ycf27_p1	ycf27	1	TTGGAGCTAAAAAGTGGCGT	ATTCTCACGCGTTACTCACC	20	20	45.0	50.0	58.0	58.0	0.0	10.2	0.032171	38276	38995	1411-1411	0.500000	59.7
ycf27_p2	ycf27	2	TTGGAGCTAAAAAGTGGCGT	CATTGCGGAAAATTCCCCAC	20	20	45.0	50.0	58.0	58.0	0.0	6.6	0.033092	38276	38995	1658-1658	0.500000	59.7
ycf27_p3	ycf27	3	TTGGAGCTAAAAAGTGGCGT	TTTACAACCCACAGGCCTTC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.034634	38276	38995	1714-1714	0.500000	59.7
ycf27_p4	ycf27	4	TTGGAGCTAAAAAGTGGCGT	AGCTACTGATCGTTGCCTTG	20	20	45.0	50.0	58.0	58.0	0.0	15.8	0.040076	38276	38995	1573-1573	0.500000	59.7
ycf27_p5	ycf27	5	TTGGAGCTAAAAAGTGGCGT	CAGCTACTGATCGTTGCCTT	20	20	45.0	50.0	58.0	58.0	0.0	15.8	0.040076	38276	38995	1574-1574	0.500000	59.7
trnL-trnE_p1	trnL-trnE	1	TTGCGTCTACCAATTCCACC	CCTAGGACACCTCCCTTTCA	20	20	50.0	55.0	58.2	58.0	0.0	1.1	0.231304	50342	50471	207-214	1.000000	88.8
trnL-trnE_p2	trnL-trnE	2	TTGCGTCTACCAATTCCACC	CTATGCCCCCATCGTCTAGA	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.259194	50342	50471	229-236	1.000000	88.3
trnL-trnE_p3	trnL-trnE	3	TTGCGTCTACCAATTCCACC	TATGCCCCCATCGTCTAGAG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.259194	50342	50471	228-235	1.000000	88.3
trnL-trnE_p4	trnL-trnE	4	TCCGTTGCGTCTACCAATTC	CCTAGGACACCTCCCTTTCA	20	20	50.0	55.0	58.3	58.0	0.0	1.1	0.330185	50342	50471	211-218	1.000000	88.0
trnL-trnE_p5	trnL-trnE	5	TCCGTTGCGTCTACCAATTC	TATGCCCCCATCGTCTAGAG	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.358075	50342	50471	232-239	1.000000	87.5
trnM-trnF_p1	trnM-trnF	1	CGGGCTCATAATCCGTCATC	TCCACTGCTCTACCAACTGA	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.294887	122424	122579	245-266	1.000000	87.5
trnM-trnF_p2	trnM-trnF	2	CGGGCTCATAATCCGTCATC	GCGGATTTTCAATCCACTGC	20	20	55.0	50.0	58.3	58.1	0.0	14.5	0.371889	122424	122579	257-278	1.000000	86.8
trnM-trnF_p3	trnM-trnF	3	CGGGCTCATAATCCGTCATC	CACGCGGATTTTCAATCCAC	20	20	55.0	50.0	58.3	58.1	0.0	12.5	0.390155	122424	122579	260-281	1.000000	86.7
trnM-trnF_p4	trnM-trnF	4	CGGGCTCATAATCCGTCATC	AGACTTGAACTCGTGACACG	20	20	55.0	50.0	58.3	57.9	0.0	21.9	0.406322	122424	122579	276-297	1.000000	86.4
trnM-trnF_p5	trnM-trnF	5	CGGGCTCATAATCCGTCATC	GACACGCGGATTTTCAATCC	20	20	55.0	50.0	58.3	57.8	0.0	12.5	0.459266	122424	122579	262-283	1.000000	86.1