primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
accD-rpl23_p1	accD-rpl23	1	CATGGTTCCACGAGCTCTTT	TTGATCCCGAATCGACATTT	20	20	50.0	40.0	58.2	55.1	12.8	3.4	3.040480	4024	5389	1471-1718	1.000000	62.7
accD-rpl23_p2	accD-rpl23	2	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATT	20	20	50.0	40.0	58.2	55.1	12.8	3.4	3.040480	4024	5389	1472-1719	1.000000	62.7
accD-rpl23_p3	accD-rpl23	3	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATTT	20	21	50.0	38.1	58.2	56.0	12.8	3.4	3.232014	4024	5389	1472-1719	1.000000	61.2
accD-rpl23_p4	accD-rpl23	4	CATGGTTCCACGAGCTCTTT	ATTTGATCCCGAATCGACAT	20	20	50.0	40.0	58.2	54.9	12.8	3.4	3.276656	4024	5389	1473-1720	1.000000	60.8
accD-rpl23_p5	accD-rpl23	5	ATGGTTCCACGAGCTCTTTT	TTGATCCCGAATCGACATTT	20	20	45.0	40.0	57.4	55.1	12.8	3.4	3.422616	4024	5389	1470-1717	1.000000	59.6
rpoA_p1	rpoA	1	TATGCTACGTGGCAATGGTC	CGTCGTCCAGTAGCTACAAC	20	20	50.0	55.0	58.1	58.1	30.6	25.3	0.159428	12530	13570	1564-1915	1.000000	85.4
rpoA_p2	rpoA	2	TATGCTACGTGGCAATGGTC	AATGGCTTCAGTACCTGCAG	20	20	50.0	50.0	58.1	57.9	30.6	6.8	0.171697	12530	13570	1637-1988	1.000000	85.0
rpoA_p3	rpoA	3	CCATTTGCTGCTCAGATTGC	CGTCGTCCAGTAGCTACAAC	20	20	50.0	55.0	58.1	58.1	0.0	25.3	0.184962	12530	13570	1641-1992	1.000000	84.9
rpoA_p4	rpoA	4	CCAGCACTGCGAGCTATTTA	CGTCGTCCAGTAGCTACAAC	20	20	50.0	55.0	58.1	58.1	0.0	25.3	0.167558	12530	13570	1533-1884	0.750000	75.0
rpoA_p5	rpoA	5	CCAGCACTGCGAGCTATTTA	AATGGCTTCAGTACCTGCAG	20	20	50.0	50.0	58.1	57.9	0.0	6.8	0.179827	12530	13570	1606-1957	0.750000	74.6
rps12-clpP_p1	rps12-clpP	1	GCATACTCCCTTCCGTTGAG	CCCGTGTAGAAGGAAAGCTC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	28081	29276	1561-1673	1.000000	83.0
rps12-clpP_p2	rps12-clpP	2	GCATACTCCCTTCCGTTGAG	CCGTGTAGAAGGAAAGCTCC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	28081	29276	1560-1672	1.000000	83.0
rps12-clpP_p3	rps12-clpP	3	GCATACTCCCTTCCGTTGAG	CGTGTAGAAGGAAAGCTCCC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	28081	29276	1559-1671	1.000000	83.0
rps12-clpP_p4	rps12-clpP	4	TTTCTTATCGGCTGTCTCGC	CCGTGTAGAAGGAAAGCTCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.088970	28081	29276	1508-1620	1.000000	82.9
rps12-clpP_p5	rps12-clpP	5	TTTCTTATCGGCTGTCTCGC	CGTGTAGAAGGAAAGCTCCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.088970	28081	29276	1507-1619	1.000000	82.9
ycf1_p1	ycf1	1	TTAGACGTAGGCCTAGTCCG	TGGATACCGAGACCAGAGTC	20	20	55.0	55.0	58.0	57.9	26.0	0.0	0.095190	35265	35384	803-860	1.000000	89.1
ycf1_p2	ycf1	2	TTAGACGTAGGCCTAGTCCG	CGAATGGGAGCAAGATTGGA	20	20	55.0	50.0	58.0	58.0	26.0	0.0	0.083644	35265	35384	480-534	0.750000	74.0
ycf1_p3	ycf1	3	TGAAAAACGTACGCTTGGGA	CGAATGGGAGCAAGATTGGA	20	20	45.0	50.0	58.0	58.0	16.7	0.0	0.091858	35265	35384	1002-1056	0.750000	73.7
ycf1_p4	ycf1	4	TCTCCAGGATGCTTGCAATC	CGAATGGGAGCAAGATTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.091130	35265	35384	1146-1200	0.750000	73.6
ycf1_p5	ycf1	5	CTCTCCAGGATGCTTGCAAT	CGAATGGGAGCAAGATTGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096918	35265	35384	1147-1201	0.750000	73.6
ycf1-trnL-CAA_p1	ycf1-trnL-CAA	1	CAAGTTCTTCTCGAGGACCG	CATTTTGAGTGTCGCGTGTC	20	20	55.0	50.0	58.0	58.1	13.6	5.8	0.078668	38751	39634	1639-1767	1.000000	85.7
ycf1-trnL-CAA_p2	ycf1-trnL-CAA	2	CAAGTTCTTCTCGAGGACCG	GACATTTTGAGTGTCGCGTG	20	20	55.0	50.0	58.0	58.1	13.6	5.8	0.078668	38751	39634	1641-1769	1.000000	85.7
ycf1-trnL-CAA_p3	ycf1-trnL-CAA	3	TGGATGAGAAGACCACCAGT	CATTTTGAGTGTCGCGTGTC	20	20	50.0	50.0	58.0	58.1	0.0	5.8	0.092668	38751	39634	1141-1269	1.000000	85.6
ycf1-trnL-CAA_p4	ycf1-trnL-CAA	4	TTTGAAGCCAAGGGTGTCTC	CATTTTGAGTGTCGCGTGTC	20	20	50.0	50.0	58.0	58.1	0.0	5.8	0.081384	38751	39634	1264-1384	0.750000	74.4
ycf1-trnL-CAA_p5	ycf1-trnL-CAA	5	TTTGAAGCCAAGGGTGTCTC	GACATTTTGAGTGTCGCGTG	20	20	50.0	50.0	58.0	58.1	0.0	5.8	0.081384	38751	39634	1266-1386	0.750000	74.4
ycf2_p1	ycf2	1	ACTTCCGTCTCTGAAAAGGG	GCAGAAGAAGAATTTGCTCGT	20	21	50.0	42.9	57.2	57.2	0.0	0.0	2.662219	46539	46658	221-227	1.000000	68.0
ycf2_p2	ycf2	2	ACTTCCGTCTCTGAAAAGGG	AGCAGAAGAAGAATTTGCTCG	20	21	50.0	42.9	57.2	56.9	0.0	0.0	2.940204	46539	46658	222-228	1.000000	65.7
ycf2_p3	ycf2	3	AACTTCCGTCTCTGAAAAGGG	GCAGAAGAAGAATTTGCTCGT	21	21	47.6	42.9	57.9	57.2	0.0	0.0	2.948202	46539	46658	222-228	1.000000	65.7
ycf2_p4	ycf2	4	CTTCCGTCTCTGAAAAGGGA	GCAGAAGAAGAATTTGCTCGT	20	21	50.0	42.9	56.9	57.2	0.0	0.0	2.951311	46539	46658	220-226	1.000000	65.7
ycf2_p5	ycf2	5	ACTTCCGTCTCTGAAAAGGG	GCAGAAGAAGAATTTGCTCGTT	20	22	50.0	40.9	57.2	57.8	0.0	0.0	2.994049	46539	46658	221-227	1.000000	65.3
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	ACCTTGACGTGGTGAAAGTC	ACTTCCCGTTTGACTTGCAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.069278	52843	53575	850-891	1.000000	84.2
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	CCTTGACGTGGTGAAAGTCA	ACTTCCCGTTTGACTTGCAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.070639	52843	53575	849-890	1.000000	84.2
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	ACCTTGACGTGGTGAAAGTC	GGGCAGGTTCTTACGCATTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.083215	52843	53575	896-937	1.000000	83.9
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	CCTTGACGTGGTGAAAGTCA	GGGCAGGTTCTTACGCATTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.084576	52843	53575	895-936	1.000000	83.9
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	ACCTTGACGTGGTGAAAGTC	AAACACCACTTCCCGTTTGA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.182310	52843	53575	857-898	1.000000	83.3
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	AGTGACTGGAGTGAAGTCGT	GCGTTGATGAGCACATTGAA	20	20	50.0	45.0	58.3	57.4	0.0	0.0	0.923623	55060	55925	959-1038	1.000000	78.4
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	AAGTCGTAACAAGGTAGCCG	GCGTTGATGAGCACATTGAA	20	20	50.0	45.0	57.6	57.4	0.0	0.0	0.976189	55060	55925	946-1025	1.000000	78.0
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	GGCTAGTGACTGGAGTGAAG	GCGTTGATGAGCACATTGAA	20	20	55.0	45.0	57.3	57.4	0.0	0.0	1.281076	55060	55925	963-1042	1.000000	75.5
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	TGCCATGCTCGAAGTCATTA	GCGTTGATGAGCACATTGAA	20	20	45.0	45.0	57.3	57.4	0.0	0.0	1.306803	55060	55925	1016-1095	1.000000	75.2
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	AGTCGTAACAAGGTAGCCGT	GCGTTGATGAGCACATTGAA	20	20	50.0	45.0	58.7	57.4	0.0	0.0	1.365999	55060	55925	945-1024	1.000000	74.8
trnA-UGC-rrn23_p1	trnA-UGC-rrn23	1	TAGACAGTGGTTGACCCTGT	GTCGATTAACACGCCCTTCT	20	20	50.0	50.0	57.9	58.0	18.2	0.0	0.103081	57784	57926	262-274	1.000000	80.7
trnA-UGC-rrn23_p2	trnA-UGC-rrn23	2	GGTTCGAGTCCGCTTATCTC	GTCGATTAACACGCCCTTCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.128533	57784	57926	229-241	1.000000	80.7
trnA-UGC-rrn23_p3	trnA-UGC-rrn23	3	GTTCGAGTCCGCTTATCTCC	GTCGATTAACACGCCCTTCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.128533	57784	57926	228-240	1.000000	80.7
trnA-UGC-rrn23_p4	trnA-UGC-rrn23	4	TAGACAGTGGTTGACCCTGT	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	57.9	58.0	18.2	3.6	0.114015	57784	57926	312-324	1.000000	80.4
trnA-UGC-rrn23_p5	trnA-UGC-rrn23	5	GTTCGAGTCCGCTTATCTCC	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	57.9	58.0	0.0	3.6	0.139468	57784	57926	278-290	1.000000	80.3
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CCACCTCAAGATGAGTGCTC	GATTGGTGTGGTTCCCCTAC	20	20	55.0	55.0	58.0	57.9	6.6	0.0	0.145632	60992	61309	471-476	1.000000	80.9
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	CCACCTCAAGATGAGTGCTC	AAGTTCGGGATGGATTGGTG	20	20	55.0	50.0	58.0	57.9	6.6	0.0	0.150126	60992	61309	483-488	1.000000	80.8
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	CCACCTCAAGATGAGTGCTC	ACCAAGTTCGGGATGGATTG	20	20	55.0	50.0	58.0	57.9	6.6	0.0	0.150126	60992	61309	486-491	1.000000	80.8
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	CCACCTCAAGATGAGTGCTC	CAAGTTCGGGATGGATTGGT	20	20	55.0	50.0	58.0	57.9	6.6	0.0	0.150126	60992	61309	484-489	1.000000	80.8
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	CCACCTCAAGATGAGTGCTC	CACCAAGTTCGGGATGGATT	20	20	55.0	50.0	58.0	57.9	6.6	0.0	0.150126	60992	61309	487-492	1.000000	80.8
rps15-ndhH_p1	rps15-ndhH	1	AGATATTCGTGGACTGCGTT	TGATGGATGATGAGGACCCA	20	20	45.0	50.0	57.0	57.8	0.0	0.0	1.156679	66674	66786	193-198	1.000000	74.8
rps15-ndhH_p2	rps15-ndhH	2	AGATATTCGTGGACTGCGTT	GGTCTTTCTCCCTAGCAACC	20	20	45.0	55.0	57.0	57.6	0.0	0.0	1.366449	66674	66786	158-163	1.000000	74.0
rps15-ndhH_p3	rps15-ndhH	3	AGATATTCGTGGACTGCGTT	AAGTCGAAGAACACCATGCA	20	20	45.0	45.0	57.0	57.7	0.0	0.0	1.216072	66674	66786	214-219	1.000000	73.9
rps15-ndhH_p4	rps15-ndhH	4	AGATATTCGTGGACTGCGTT	AGTCGAAGAACACCATGCAT	20	20	45.0	45.0	57.0	57.5	0.0	0.0	1.441092	66674	66786	213-218	1.000000	72.2
rps15-ndhH_p5	rps15-ndhH	5	AGATATTCGTGGACTGCGTT	TGAGGTCTTTCTCCCTAGCA	20	20	45.0	50.0	57.0	57.4	0.0	0.0	1.584944	66674	66786	161-166	1.000000	72.2
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	AATAAACCGGACCAACCCAC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.1	58.3	9.5	0.0	0.369028	88618	89482	1006-1013	1.000000	78.4
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CAATAAACCGGACCAACCCA	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.1	58.3	9.5	0.0	0.370389	88618	89482	1007-1014	1.000000	78.4
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	AATAAACCGGACCAACCCAC	TAAGTCATCGGTTCAAGCCC	20	20	50.0	50.0	58.1	57.6	9.5	0.0	0.482812	88618	89482	1002-1009	1.000000	77.5
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CAATAAACCGGACCAACCCA	TAAGTCATCGGTTCAAGCCC	20	20	50.0	50.0	58.1	57.6	9.5	0.0	0.484173	88618	89482	1003-1010	1.000000	77.5
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	ACAAAACGGTCTCTGCGTAG	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.503237	88618	89482	982-989	1.000000	77.4
psbM-petN_p1	psbM-petN	1	GTCATTTACGCGCAAACGAA	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	58.1	58.1	6.7	0.0	0.180456	90829	91431	714-738	1.000000	80.3
psbM-petN_p2	psbM-petN	2	TCATTTACGCGCAAACGAAG	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.8	58.1	6.7	0.0	0.340740	90829	91431	713-737	1.000000	79.0
psbM-petN_p3	psbM-petN	3	GCAAACGAAGCCTCAAAGTG	TGGGCTGCTCTAATGGTAGT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.361642	90829	91431	703-727	1.000000	78.9
psbM-petN_p4	psbM-petN	4	TATTCATTGCAGTGCCCACT	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.5	58.1	13.7	0.0	0.633117	90829	91431	746-770	1.000000	76.6
psbM-petN_p5	psbM-petN	5	ACGAAGCCTCAAAGTGAACT	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.742489	90829	91431	699-723	1.000000	75.9
trnG-UCC_p1	trnG-UCC	1	TCACAACGAGATTCGGGAAC	TGATGATCGGAACTTGCATT	20	20	50.0	40.0	57.9	55.4	3.4	0.0	2.648122	108291	109126	1023-1055	1.000000	60.8
trnG-UCC_p2	trnG-UCC	2	TCACAACGAGATTCGGGAAC	ATGATGATCGGAACTTGCAT	20	20	50.0	40.0	57.9	55.2	3.4	0.0	2.883562	108291	109126	1024-1056	1.000000	58.9
trnG-UCC_p3	trnG-UCC	3	TCACAACGAGATTCGGGAAC	ATGATGATCGGAACTTGCATT	20	21	50.0	38.1	57.9	56.0	3.4	0.0	3.073044	108291	109126	1024-1056	1.000000	57.4
trnG-UCC_p4	trnG-UCC	4	TCACAACGAGATTCGGGAAC	TGATGATCGGAACTTGCATTAGA	20	23	50.0	39.1	57.9	58.0	3.4	0.0	3.096829	108291	109126	1023-1055	1.000000	57.2
trnG-UCC_p5	trnG-UCC	5	TTCACAACGAGATTCGGGAA	TGATGATCGGAACTTGCATT	20	20	45.0	40.0	57.2	55.4	3.4	0.0	3.397500	108291	109126	1024-1056	1.000000	54.8