primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	GATCCACTTGGCTACATCCG	TTTGCATCAACGATTTGGCC	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.206333	400	1461	1887-1892	1.000000	79.0
psbA_p2	psbA	2	CAATCCACTGCCTTGATCCA	TTTGCATCAACGATTTGGCC	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.210694	400	1461	1901-1906	1.000000	78.9
psbA_p3	psbA	3	AATCCACTGCCTTGATCCAC	TTTGCATCAACGATTTGGCC	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.212202	400	1461	1900-1905	1.000000	78.9
psbA_p4	psbA	4	ACAATCCACTGCCTTGATCC	TTTGCATCAACGATTTGGCC	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.212202	400	1461	1902-1907	1.000000	78.9
psbA_p5	psbA	5	TTTATATGGGCGAACGACGG	TTTGCATCAACGATTTGGCC	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.221900	400	1461	1948-1953	1.000000	78.8
matK_p1	matK	1	CCATTTACATGGTGTCAGAACC	CCTTGTTTTGACTGTATCGCA	22	21	45.5	42.9	57.3	56.8	0.0	0.0	4.869443	2073	3584	1620-1620	1.000000	41.5
matK_p2	matK	2	CCATTTACATGGTGTCAGAACC	ACCTTGTTTTGACTGTATCGC	22	21	45.5	42.9	57.3	56.8	0.0	0.0	4.871477	2073	3584	1621-1621	1.000000	41.5
matK_p3	matK	3	CCATTTACATGGTGTCAGAACC	CCTTGTTTTGACTGTATCGCAC	22	22	45.5	45.5	57.3	58.2	0.0	0.0	4.891582	2073	3584	1620-1620	1.000000	41.3
matK_p4	matK	4	CCATTTACATGGTGTCAGAACC	ACCTTGTTTTGACTGTATCGCA	22	22	45.5	40.9	57.3	58.5	0.0	0.0	5.229304	2073	3584	1621-1621	1.000000	40.4
matK_p5	matK	5	CCATTTACATGGTGTCAGAACC	TGTTTTGACTGTATCGCACT	22	20	45.5	40.0	57.3	55.3	0.0	0.0	5.373403	2073	3584	1617-1617	1.000000	40.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6377	7766	1450-1467	1.000000	79.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6377	7766	1461-1478	1.000000	79.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GACGACCAACCACTACGAAT	20	20	45.0	50.0	58.1	57.6	8.1	0.0	0.498263	6377	7766	1546-1563	1.000000	77.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	GACGACCAACCACTACGAAT	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.563171	6377	7766	1557-1574	1.000000	76.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6377	7766	1454-1471	1.000000	75.1
trnR-atpA_p1	trnR-atpA	1	TGGAATGAAAAGCGTCCATTG	GGAAGCCCTTTTGAAAGAAGC	21	21	42.9	47.6	57.4	58.0	5.3	5.1	2.606301	10983	11538	706-715	1.000000	60.7
trnR-atpA_p2	trnR-atpA	2	GGAATGAAAAGCGTCCATTGT	GGAAGCCCTTTTGAAAGAAGC	21	21	42.9	47.6	57.4	58.0	5.3	5.1	2.607983	10983	11538	705-714	1.000000	60.7
trnR-atpA_p3	trnR-atpA	3	GGTTCAAATCCTATTGGACGC	GGAAGCCCTTTTGAAAGAAGC	21	21	47.6	47.6	57.3	58.0	0.0	5.1	2.724808	10983	11538	645-654	1.000000	59.9
trnR-atpA_p4	trnR-atpA	4	TGGAATGAAAAGCGTCCATTG	TCAGGAACAGATGGAACGTT	21	20	42.9	45.0	57.4	56.8	5.3	0.0	2.795873	10983	11538	682-691	1.000000	59.3
trnR-atpA_p5	trnR-atpA	5	TGGAATGAAAAGCGTCCATTG	TTCAGGAACAGATGGAACGT	21	20	42.9	45.0	57.4	56.8	5.3	0.0	2.795873	10983	11538	683-692	1.000000	59.3
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	15035	15985	1017-1039	1.000000	81.8
atpH-atpI_p2	atpH-atpI	2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.172323	15035	15985	1102-1124	1.000000	81.6
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.0	57.8	1.4	21.3	0.214265	15035	15985	987-1009	1.000000	81.6
atpH-atpI_p4	atpH-atpI	4	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	20	20	55.0	55.0	58.0	57.8	0.0	21.3	0.215461	15035	15985	1072-1094	1.000000	81.3
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	15035	15985	1018-1040	1.000000	80.6
trnE-trnT_p1	trnE-trnT	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	33203	34186	1124-1126	1.000000	79.4
trnE-trnT_p2	trnE-trnT	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	33203	34186	1103-1105	1.000000	78.9
trnE-trnT_p3	trnE-trnT	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	33203	34186	1100-1102	1.000000	78.8
trnE-trnT_p4	trnE-trnT	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33203	34186	1086-1088	1.000000	78.6
trnE-trnT_p5	trnE-trnT	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33203	34186	1065-1067	1.000000	78.5
psbZ-trnG_p1	psbZ-trnG	1	CGTTGCATTTGCTTCTCCTG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.0	58.1	3.9	0.0	0.085537	38620	39167	717-724	1.000000	80.7
psbZ-trnG_p2	psbZ-trnG	2	CGTTGCATTTGCTTCTCCTG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.0	58.1	3.9	0.0	0.085537	38620	39167	718-725	1.000000	80.6
psbZ-trnG_p3	psbZ-trnG	3	TTGGTCGGCGAACAAAAATG	AATCGAACCCGCATCTTCTC	20	20	45.0	50.0	57.9	58.1	35.5	0.0	0.185589	38620	39167	693-700	1.000000	79.9
psbZ-trnG_p4	psbZ-trnG	4	TTGGTCGGCGAACAAAAATG	GAATCGAACCCGCATCTTCT	20	20	45.0	50.0	57.9	58.1	35.5	0.0	0.185589	38620	39167	694-701	1.000000	79.9
psbZ-trnG_p5	psbZ-trnG	5	GGTCGGCGAACAAAAATGTT	AATCGAACCCGCATCTTCTC	20	20	45.0	50.0	57.9	58.1	21.9	0.0	0.187042	38620	39167	691-698	1.000000	79.9
trnT-trnL_p1	trnT-trnL	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	49300	50361	1119-1123	1.000000	78.0
trnT-trnL_p2	trnT-trnL	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	49300	50361	1151-1155	1.000000	77.1
trnT-trnL_p3	trnT-trnL	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	49300	50361	1113-1117	1.000000	76.7
trnT-trnL_p4	trnT-trnL	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	49300	50361	1151-1155	1.000000	75.9
trnT-trnL_p5	trnT-trnL	5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	49300	50361	1145-1149	1.000000	75.8
trnL-trnF_p1	trnL-trnF	1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	50914	52194	863-1371	1.000000	84.6
trnL-trnF_p2	trnL-trnF	2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	50914	52194	860-1368	1.000000	81.4
trnL-trnF_p3	trnL-trnF	3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.0	58.1	0.0	20.1	1.013432	50914	52194	852-1360	1.000000	80.8
trnL-trnF_p4	trnL-trnF	4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	20	20	50.0	55.0	57.5	58.6	0.0	0.0	1.064405	50914	52194	825-1333	1.000000	80.5
trnL-trnF_p5	trnL-trnF	5	AATCGTGAGGGTTCAAGTCC	TCATGATGTGCCAAGAACCA	20	20	50.0	45.0	57.5	57.4	0.0	0.0	1.057434	50914	52194	885-1393	1.000000	80.3
trnM-atpE_p1	trnM-atpE	1	CGATGTGATAGGGCCCATTT	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	57.7	58.0	27.6	0.0	0.307991	56344	56512	338-338	1.000000	79.9
trnM-atpE_p2	trnM-atpE	2	CGATGTGATAGGGCCCATTT	CGAGCTAGGACACGAGTAGA	20	20	50.0	55.0	57.7	58.1	27.6	0.0	0.339454	56344	56512	330-330	1.000000	79.7
trnM-atpE_p3	trnM-atpE	3	TATCGATGTGATAGGGCCCA	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	57.7	58.0	27.6	0.0	0.350283	56344	56512	341-341	1.000000	79.5
trnM-atpE_p4	trnM-atpE	4	ATCGATGTGATAGGGCCCAT	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	58.3	58.0	27.6	0.0	0.369795	56344	56512	340-340	1.000000	79.4
trnM-atpE_p5	trnM-atpE	5	TATCGATGTGATAGGGCCCA	CGAGCTAGGACACGAGTAGA	20	20	50.0	55.0	57.7	58.1	27.6	0.0	0.381747	56344	56512	333-333	1.000000	79.3
atpE_p1	atpE	1	GCTATAGCCCTTGTGCTTGT	GGAAATACGTTGGGCTAGCA	20	20	50.0	50.0	58.0	58.0	14.1	18.2	0.076803	56513	56923	986-986	1.000000	80.6
atpE_p2	atpE	2	GCTATAGCCCTTGTGCTTGT	GCACGAGCACGAAAAATTGA	20	20	50.0	45.0	58.0	58.0	14.1	11.4	0.075159	56513	56923	1059-1059	1.000000	80.5
atpE_p3	atpE	3	GCTATAGCCCTTGTGCTTGT	AGCACGAGCACGAAAAATTG	20	20	50.0	45.0	58.0	58.0	14.1	11.4	0.080679	56513	56923	1060-1060	1.000000	80.5
atpE_p4	atpE	4	GCTATAGCCCTTGTGCTTGT	GCAGTTTATGTACCTGCGGA	20	20	50.0	50.0	58.0	58.0	14.1	5.5	0.062665	56513	56923	1347-1347	1.000000	80.4
atpE_p5	atpE	5	TAAATGGGGCACTCGATTCG	GCAGTTTATGTACCTGCGGA	20	20	50.0	50.0	58.1	58.0	0.0	5.5	0.070438	56513	56923	1528-1528	1.000000	80.3
atpB-rbcL_p1	atpB-rbcL	1	TTTGAGCGATACGTCCCAAG	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.021848	58417	59210	910-919	1.000000	73.2
atpB-rbcL_p2	atpB-rbcL	2	TTTGAGCGATACGTCCCAAG	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.055135	58417	59210	909-918	1.000000	73.0
atpB-rbcL_p3	atpB-rbcL	3	GATTTGAGCGATACGTCCCA	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.8	58.0	0.0	0.0	1.236218	58417	59210	912-921	1.000000	71.5
atpB-rbcL_p4	atpB-rbcL	4	TGATTTGAGCGATACGTCCC	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.8	58.0	0.0	0.0	1.236218	58417	59210	913-922	1.000000	71.5
atpB-rbcL_p5	atpB-rbcL	5	GATTTGAGCGATACGTCCCA	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	57.8	58.1	0.0	0.0	1.269506	58417	59210	911-920	1.000000	71.2
accD_p1	accD	1	AATAGGGGCAAGAAAGCTCT	AACTACTTGTTCGTCACAAA	20	20	45.0	35.0	56.5	53.0	0.0	4.8	6.475556	61353	62948	1647-1671	1.000000	42.2
accD_p2	accD	2	AATAGGGGCAAGAAAGCTCT	ACTACTTGTTCGTCACAAAA	20	20	45.0	35.0	56.5	53.0	0.0	4.8	6.475556	61353	62948	1646-1670	1.000000	42.2
accD_p3	accD	3	AATAGGGGCAAGAAAGCTCT	AACTACTTGTTCGTCACAAAA	20	21	45.0	33.3	56.5	53.9	0.0	4.8	6.573441	61353	62948	1647-1671	1.000000	42.2
accD_p4	accD	4	AATAGGGGCAAGAAAGCTCT	ACTACTTGTTCGTCACAAAAA	20	21	45.0	33.3	56.5	53.9	0.0	4.8	6.573441	61353	62948	1646-1670	1.000000	42.2
accD_p5	accD	5	AAATAGGGGCAAGAAAGCTCT	ACTACTTGTTCGTCACAAAA	21	20	42.9	35.0	57.3	53.0	0.0	4.8	6.711534	61353	62948	1647-1671	1.000000	42.2
rpl32-trnL_p1	rpl32-trnL	1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.437017	117095	117878	907-937	1.000000	80.0
rpl32-trnL_p2	rpl32-trnL	2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.438388	117095	117878	908-938	1.000000	79.9
rpl32-trnL_p3	rpl32-trnL	3	AGCGTTGAAAGCTTTTTCGC	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.5	58.0	30.2	0.0	0.530725	117095	117878	904-934	1.000000	79.2
rpl32-trnL_p4	rpl32-trnL	4	GCGTTGAAAGCTTTTTCGCT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.5	58.0	30.1	0.0	0.530725	117095	117878	903-933	1.000000	79.2
rpl32-trnL_p5	rpl32-trnL	5	GCGTTGAAAGCTTTTTCGCT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.5	58.0	30.1	0.0	0.532096	117095	117878	904-934	1.000000	79.2
ycf1_p1	ycf1	1	TTTCGGGGAGAACCAAAAGG	CCTTACCGCAGACGAATCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014876	131167	131286	1283-1304	1.000000	84.6
ycf1_p2	ycf1	2	TTTCGGGGAGAACCAAAAGG	AGCTCGAGTAGGGGATGTAC	20	20	50.0	55.0	58.0	58.0	0.0	15.1	0.026228	131167	131286	1383-1404	1.000000	84.3
ycf1_p3	ycf1	3	TTTCGGGGAGAACCAAAAGG	GCTCGAGTAGGGGATGTACT	20	20	50.0	55.0	58.0	58.0	0.0	15.1	0.026228	131167	131286	1382-1403	1.000000	84.3
ycf1_p4	ycf1	4	TTTCGGGGAGAACCAAAAGG	CAGCTCGAGTAGGGGATGTA	20	20	50.0	55.0	58.0	58.0	0.0	15.1	0.027633	131167	131286	1384-1405	1.000000	84.2
ycf1_p5	ycf1	5	TTTCGGGGAGAACCAAAAGG	TGCCAAAGTGACGGAAAAGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.055855	131167	131286	1598-1619	1.000000	83.5