primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ycf4	1	GGCTATATGGTCTTTCCCCC	CGGGGTGGGAGTCAAAATTT	20	20	55.0	50.0	57.1	58.4	0.0	0.0	1.253247	2466	3020	719-719	1.000000	70.0
ycf4_p2	ycf4	2	GGCTATATGGTCTTTCCCCC	ATGAGAATGACTTCGGGGTG	20	20	55.0	50.0	57.1	57.3	0.0	0.0	1.579965	2466	3020	732-732	1.000000	67.4
ycf4_p3	ycf4	3	CTGGCTATATGGTCTTTCCCC	CGGGGTGGGAGTCAAAATTT	21	20	52.4	50.0	57.6	58.4	0.0	0.0	1.797298	2466	3020	721-721	1.000000	65.6
ycf4_p4	ycf4	4	GGCTATATGGTCTTTCCCCC	GGGGTGGGAGTCAAAATTTG	20	20	55.0	50.0	57.1	56.9	0.0	0.0	2.009501	2466	3020	718-718	1.000000	63.9
ycf4_p5	ycf4	5	TGGCTATATGGTCTTTCCCC	CGGGGTGGGAGTCAAAATTT	20	20	50.0	50.0	56.4	58.4	0.0	0.0	2.016134	2466	3020	720-720	1.000000	63.9
rpl22_p1	rpl22	1	TAGCTCGAAGGGTCTGTAGG	TTCCAGGGACATGCGAAAAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.065939	15004	15384	1226-1226	1.000000	79.9
rpl22_p2	rpl22	2	TAGCTCGAAGGGTCTGTAGG	TTTCCAGGGACATGCGAAAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.065939	15004	15384	1227-1227	1.000000	79.9
rpl22_p3	rpl22	3	TAGCTCGAAGGGTCTGTAGG	TTTTCCAGGGACATGCGAAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.065939	15004	15384	1228-1228	1.000000	79.9
rpl22_p4	rpl22	4	TAGCTCGAAGGGTCTGTAGG	TCAAAACGTTGGCTGGGTAA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.108464	15004	15384	1718-1718	1.000000	79.5
rpl22_p5	rpl22	5	TAGCTCGAAGGGTCTGTAGG	ATAGTAACCTGGTCCCGGAG	20	20	55.0	55.0	57.9	57.9	0.0	0.7	0.137860	15004	15384	1373-1373	1.000000	79.3
rpl22-rps19_p1	rpl22-rps19	1	ATGAGCTACTCTACGGGCTT	TTCCAGGGACATGCGAAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.086435	15385	15463	220-220	0.500000	59.3
rpl22-rps19_p2	rpl22-rps19	2	ATGAGCTACTCTACGGGCTT	TTTCCAGGGACATGCGAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.086435	15385	15463	221-221	0.500000	59.3
rpl22-rps19_p3	rpl22-rps19	3	ATGAGCTACTCTACGGGCTT	TTTTCCAGGGACATGCGAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.086435	15385	15463	222-222	0.500000	59.3
rpl22-rps19_p4	rpl22-rps19	4	ATGAGCTACTCTACGGGCTT	TCAAAACGTTGGCTGGGTAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.128961	15385	15463	712-712	0.500000	59.0
rpl22-rps19_p5	rpl22-rps19	5	ATGAGCTACTCTACGGGCTT	ATAGTAACCTGGTCCCGGAG	20	20	50.0	55.0	57.9	57.9	0.0	0.7	0.158357	15385	15463	367-367	0.500000	58.7
psbJ-accD_p1	psbJ-accD	1	GATAGGCCTTTTTGCGGGTA	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	58.0	58.2	12.6	12.8	0.233488	23567	25877	2469-2494	1.000000	78.3
psbJ-accD_p2	psbJ-accD	2	CCCTCTTTGGTTGATAGGCC	CATGGTTCCACGAGCTCTTT	20	20	55.0	50.0	57.9	58.2	0.0	12.8	0.245080	23567	25877	2481-2506	1.000000	78.2
psbJ-accD_p3	psbJ-accD	3	ACGGGATTAGGGTCTTCCTT	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	57.7	58.2	0.0	12.8	0.452013	23567	25877	2405-2430	1.000000	76.6
psbJ-accD_p4	psbJ-accD	4	GGTTGATAGGCCTTTTTGCG	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	57.7	58.2	12.6	12.8	0.464513	23567	25877	2473-2498	1.000000	76.5
psbJ-accD_p5	psbJ-accD	5	GTTGATAGGCCTTTTTGCGG	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	57.7	58.2	12.6	12.8	0.464513	23567	25877	2472-2497	1.000000	76.5
accD-rbcL_p1	accD-rbcL	1	AAGGGTCTCACAATTCTCGC	GGTAATCAAGTGATCCGCGA	20	20	50.0	50.0	57.9	57.8	0.0	8.2	0.319558	27981	28749	997-997	1.000000	77.5
accD-rbcL_p2	accD-rbcL	2	AAGGGTCTCACAATTCTCGC	AGGTAATCAAGTGATCCGCG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.325673	27981	28749	998-998	1.000000	77.5
accD-rbcL_p3	accD-rbcL	3	TTTCTACGGGGGTTGTTTCG	GGTAATCAAGTGATCCGCGA	20	20	50.0	50.0	58.1	57.8	0.0	8.2	0.337362	27981	28749	935-935	1.000000	77.4
accD-rbcL_p4	accD-rbcL	4	TTTCTACGGGGGTTGTTTCG	AGGTAATCAAGTGATCCGCG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.343477	27981	28749	936-936	1.000000	77.3
accD-rbcL_p5	accD-rbcL	5	GGTCTCACAATTCTCGCACA	GGTAATCAAGTGATCCGCGA	20	20	50.0	50.0	58.2	57.8	0.0	8.2	0.423185	27981	28749	994-994	1.000000	76.7
ndhJ-chlN_p1	ndhJ-chlN	1	GTTGGATTGGGTGGCCTTTA	GACTTGGGTCCAACCACTTT	20	20	50.0	50.0	58.1	57.9	3.3	0.0	0.135730	36648	37175	632-632	1.000000	79.0
ndhJ-chlN_p2	ndhJ-chlN	2	TTGGATTGGGTGGCCTTTAC	GACTTGGGTCCAACCACTTT	20	20	50.0	50.0	58.1	57.9	3.3	0.0	0.135730	36648	37175	631-631	1.000000	79.0
ndhJ-chlN_p3	ndhJ-chlN	3	GTTGGATTGGGTGGCCTTTA	CCTCTACGGCGTCAGAAAAA	20	20	50.0	50.0	58.1	57.9	3.3	19.2	0.142912	36648	37175	659-659	1.000000	79.0
ndhJ-chlN_p4	ndhJ-chlN	4	TTGGATTGGGTGGCCTTTAC	CCTCTACGGCGTCAGAAAAA	20	20	50.0	50.0	58.1	57.9	3.3	19.2	0.142912	36648	37175	658-658	1.000000	79.0
ndhJ-chlN_p5	ndhJ-chlN	5	TTGGATTGGGTGGCCTTTAC	ACTTGGGTCCAACCACTTTG	20	20	50.0	50.0	58.1	58.2	3.3	0.0	0.292980	36648	37175	630-630	1.000000	77.8
atpI-atpH_p1	atpI-atpH	1	CGAATCCATGGAGGGTCATC	CCTACGGATAATCCAGCAGC	20	20	55.0	55.0	57.8	57.9	21.3	0.0	0.261932	78315	78891	655-655	1.000000	78.0
atpI-atpH_p2	atpI-atpH	2	CGAATCCATGGAGGGTCATC	GCCTACGGATAATCCAGCAG	20	20	55.0	55.0	57.8	57.9	21.3	0.0	0.261932	78315	78891	656-656	1.000000	78.0
atpI-atpH_p3	atpI-atpH	3	CGAATCCATGGAGGGTCATC	AACGGAAGCAGCAGAAATCA	20	20	55.0	45.0	57.8	58.1	21.3	0.0	0.279658	78315	78891	632-632	1.000000	77.9
atpI-atpH_p4	atpI-atpH	4	CGAATCCATGGAGGGTCATC	ACGGAAGCAGCAGAAATCAA	20	20	55.0	45.0	57.8	58.1	21.3	0.0	0.279658	78315	78891	631-631	1.000000	77.9
atpI-atpH_p5	atpI-atpH	5	CGAATCCATGGAGGGTCATC	GCCAGGTCCAATAGAAGCAA	20	20	55.0	50.0	57.8	57.9	21.3	0.0	0.304648	78315	78891	677-677	1.000000	77.7
atpF_p1	atpF	1	CGGGACCTGGGACTTACTAA	ACTGATTTCATCGGGTCGAA	20	20	55.0	45.0	58.1	56.9	0.0	0.0	1.194280	79457	80700	1444-1444	1.000000	70.5
atpF_p2	atpF	2	CGGGACCTGGGACTTACTAA	ACTACTGATTTCATCGGGTCG	20	21	55.0	47.6	58.1	57.6	0.0	0.0	1.581668	79457	80700	1447-1447	1.000000	67.4
atpF_p3	atpF	3	CGGGACCTGGGACTTACTAA	ACTGATTTCATCGGGTCGAAT	20	21	55.0	42.9	58.1	57.5	0.0	0.0	1.687051	79457	80700	1444-1444	1.000000	66.6
atpF_p4	atpF	4	CGGGACCTGGGACTTACTAA	CTACTGATTTCATCGGGTCGA	20	21	55.0	47.6	58.1	57.3	0.0	0.0	1.853697	79457	80700	1446-1446	1.000000	65.2
atpF_p5	atpF	5	CGGGACCTGGGACTTACTAA	CATCGGGTCGAATAGTTACCA	20	21	55.0	47.6	58.1	57.3	0.0	0.0	1.864321	79457	80700	1436-1436	1.000000	65.1
CrjaCt019-psaM_p1	CrjaCt019-psaM	1	ACGAGAGTTGAAAGATCCTTCA	AGCAGCTAAAAGGGCTATCA	22	20	40.9	45.0	57.1	56.6	2.5	0.0	4.278200	83433	83969	658-658	1.000000	45.8
CrjaCt019-psaM_p2	CrjaCt019-psaM	2	ACGAGAGTTGAAAGATCCTTCA	AAGCAGCTAAAAGGGCTATCA	22	21	40.9	42.9	57.1	57.3	2.5	0.0	4.525803	83433	83969	659-659	1.000000	43.8
CrjaCt019-psaM_p3	CrjaCt019-psaM	3	ACGAGAGTTGAAAGATCCTTCA	AGCAGCTAAAAGGGCTATCAA	22	21	40.9	42.9	57.1	57.3	2.5	0.0	4.525803	83433	83969	658-658	1.000000	43.8
CrjaCt019-psaM_p4	CrjaCt019-psaM	4	ACGAGAGTTGAAAGATCCTTCAA	AGCAGCTAAAAGGGCTATCA	23	20	39.1	45.0	57.8	56.6	14.2	0.0	4.619706	83433	83969	658-658	1.000000	43.0
CrjaCt019-psaM_p5	CrjaCt019-psaM	5	ACGAGAGTTGAAAGATCCTTCAA	AGCAGCTAAAAGGGCTATCAA	23	21	39.1	42.9	57.8	57.3	14.2	0.0	4.867309	83433	83969	658-658	1.000000	41.1
CrjaCt020-psbI_p1	CrjaCt020-psbI	1	TCTAGGAGAGATGGCTGAGC	CGAAATCCTGGGCGTAAAGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.024586	84318	84400	196-196	1.000000	80.6
CrjaCt020-psbI_p2	CrjaCt020-psbI	2	GGGTTCGAATCCCTCTCTCT	CGAAATCCTGGGCGTAAAGA	20	20	55.0	50.0	58.2	58.0	17.2	0.0	0.235471	84318	84400	127-127	1.000000	79.4
CrjaCt020-psbI_p3	CrjaCt020-psbI	3	GACTAAAGCGGTGGATTGCT	CGAAATCCTGGGCGTAAAGA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.279475	84318	84400	175-175	1.000000	78.7
CrjaCt020-psbI_p4	CrjaCt020-psbI	4	TCTAGGAGAGATGGCTGAGC	ACGAAATCCTGGGCGTAAAG	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.271881	84318	84400	197-197	1.000000	78.6
CrjaCt020-psbI_p5	CrjaCt020-psbI	5	TCTAGGAGAGATGGCTGAGC	GACGAAATCCTGGGCGTAAA	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.276760	84318	84400	198-198	1.000000	78.6
ndhA_p1	ndhA	1	GCAACCCTCTTCACGAAGAA	TTGCAGATCCCTCCTCAACT	20	20	50.0	50.0	58.1	58.3	10.5	0.0	0.459584	91267	93131	1998-1999	1.000000	76.4
ndhA_p2	ndhA	2	GCAACCCTCTTCACGAAGAA	AGATCCCTCCTCAACTGGTT	20	20	50.0	50.0	58.1	57.6	10.5	0.0	0.471909	91267	93131	1994-1995	1.000000	76.3
ndhA_p3	ndhA	3	GCAACCCTCTTCACGAAGAA	CAGATCCCTCCTCAACTGGT	20	20	50.0	55.0	58.1	58.4	10.5	0.0	0.543311	91267	93131	1995-1996	1.000000	75.7
ndhA_p4	ndhA	4	ATTGCAACCCTCTTCACGAA	TTGCAGATCCCTCCTCAACT	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.608889	91267	93131	2001-2002	1.000000	75.2
ndhA_p5	ndhA	5	GCAACCCTCTTCACGAAGAA	TTTGCAGATCCCTCCTCAAC	20	20	50.0	50.0	58.1	57.5	10.5	0.0	0.617945	91267	93131	1999-2000	1.000000	75.1
ndhF_p1	ndhF	1	GGATCTAGTGGATTGGAAACCA	CCAGGGAACGATATTGAAAAAGG	22	23	45.5	43.5	57.5	57.8	0.0	0.0	5.752584	96166	98391	2378-2378	1.000000	40.0
ndhF_p2	ndhF	2	AGGATCTAGTGGATTGGAAACC	CCAGGGAACGATATTGAAAAAGG	22	23	45.5	43.5	57.2	57.8	0.0	0.0	6.028863	96166	98391	2379-2379	1.000000	40.0
ndhF_p3	ndhF	3	TGGAAACCAAGAATGTGGAA	CCAGGGAACGATATTGAAAAAGG	20	23	40.0	43.5	55.0	57.8	0.0	0.0	6.199201	96166	98391	2365-2365	1.000000	40.0
ndhF_p4	ndhF	4	TTGGAAACCAAGAATGTGGA	CCAGGGAACGATATTGAAAAAGG	20	23	40.0	43.5	55.0	57.8	0.0	0.0	6.199201	96166	98391	2366-2366	1.000000	40.0
ndhF_p5	ndhF	5	TGTTCTCTTCAGGATCTAGTGGA	CCAGGGAACGATATTGAAAAAGG	23	23	43.5	43.5	58.1	57.8	0.0	0.0	6.313292	96166	98391	2389-2389	1.000000	40.0
ndhF-CrjaCt024_p1	ndhF-CrjaCt024	1	ATGAGATGCTTAGGAGGGGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	58.3	57.7	0.0	0.0	0.571029	98392	99193	983-983	1.000000	75.7
ndhF-CrjaCt024_p2	ndhF-CrjaCt024	2	GCTTAGGAGGGGGACGTATA	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.7	57.7	0.0	0.0	0.610105	98392	99193	976-976	1.000000	75.4
ndhF-CrjaCt024_p3	ndhF-CrjaCt024	3	ATGAGATGCTTAGGAGGGGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	58.3	57.6	0.0	0.0	0.633790	98392	99193	945-945	1.000000	75.2
ndhF-CrjaCt024_p4	ndhF-CrjaCt024	4	GCTTAGGAGGGGGACGTATA	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.7	57.6	0.0	0.0	0.672866	98392	99193	938-938	1.000000	74.9
ndhF-CrjaCt024_p5	ndhF-CrjaCt024	5	AGGAGAGGGAATGAGATGCT	TTCCTCAGTAGCTCAGTGGT	20	20	50.0	50.0	57.5	57.7	0.0	0.0	0.803878	98392	99193	993-993	1.000000	73.8
CrjaCt026_p1	CrjaCt026	1	TTCTGATTGATCTGCTCCGC	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.059195	103721	104565	979-979	1.000000	79.6
CrjaCt026_p2	CrjaCt026	2	TTCTGATTGATCTGCTCCGC	CCACCCATTATGCGCTATGT	20	20	50.0	50.0	58.1	58.1	0.0	9.7	0.158393	103721	104565	934-934	1.000000	78.8
CrjaCt026_p3	CrjaCt026	3	TTCTGATTGATCTGCTCCGC	GGATAGCCCACCCATTATGC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.215189	103721	104565	941-941	1.000000	78.4
CrjaCt026_p4	CrjaCt026	4	TTCTGATTGATCTGCTCCGC	CTGGTTCAAGCCCAGGATAG	20	20	50.0	55.0	58.1	57.7	0.0	0.0	0.393189	103721	104565	955-955	1.000000	76.9
CrjaCt026_p5	CrjaCt026	5	TTCTGATTGATCTGCTCCGC	GATAGCCCACCCATTATGCG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.601458	103721	104565	940-940	1.000000	75.3
CrjaCt030-ycf1_p1	CrjaCt030-ycf1	1	GAATGTCGCGTGTCTACCAT	TCCAAGGCAACAGAAAAGCT	20	20	50.0	45.0	58.1	57.9	5.8	0.0	0.149207	121185	122438	1203-1313	1.000000	83.3
CrjaCt030-ycf1_p2	CrjaCt030-ycf1	2	GAATGTCGCGTGTCTACCAT	GGGACCAATTGGCAATGTTG	20	20	50.0	50.0	58.1	57.9	5.8	24.8	0.175109	121185	122438	1274-1384	1.000000	83.0
CrjaCt030-ycf1_p3	CrjaCt030-ycf1	3	GAATGTCGCGTGTCTACCAT	GCTCCTTTGTATCCAAGGCA	20	20	50.0	50.0	58.1	57.9	5.8	0.0	0.206200	121185	122438	1214-1324	1.000000	82.8
CrjaCt030-ycf1_p4	CrjaCt030-ycf1	4	GAATGTCGCGTGTCTACCAT	TGCTCCTTTGTATCCAAGGC	20	20	50.0	50.0	58.1	57.9	5.8	0.0	0.206200	121185	122438	1215-1325	1.000000	82.8
CrjaCt030-ycf1_p5	CrjaCt030-ycf1	5	GCGTGTCTACCATTTCACCA	TCCAAGGCAACAGAAAAGCT	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.268443	121185	122438	1196-1306	1.000000	82.3