primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GGTATCTGGTTTACCGCGTT	20	20	55.0	50.0	58.1	58.0	0.0	4.9	0.143255	76	350	476-520	1.000000	87.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GGTATCTGGTTTACCGCGTT	20	20	50.0	50.0	57.9	58.0	0.0	4.9	0.147617	76	350	490-534	1.000000	87.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GGTATCTGGTTTACCGCGTT	20	20	50.0	50.0	57.9	58.0	0.0	4.9	0.149124	76	350	489-533	1.000000	87.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GGTATCTGGTTTACCGCGTT	20	20	50.0	50.0	57.9	58.0	0.0	4.9	0.149124	76	350	491-535	1.000000	87.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCAATTGGTCAGGGAAGCTT	20	20	55.0	50.0	58.1	58.1	0.0	16.9	0.200052	76	350	836-876	0.750000	75.4
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	AGCCGAGTACTCTACCATTGA	TTTGAATTCCCAGCAATGGA	21	20	47.6	40.0	58.0	55.5	20.3	2.3	3.524984	4345	5278	956-996	1.000000	62.0
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AGCCGAGTACTCTACCATTGA	TGAATTCCCAGCAATGGATACT	21	22	47.6	40.9	58.0	57.5	20.3	2.3	3.539106	4345	5278	954-994	1.000000	61.9
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	AGCCGAGTACTCTACCATTGA	TTGAATTCCCAGCAATGGAT	21	20	47.6	40.0	58.0	55.2	20.3	2.3	3.764330	4345	5278	955-995	1.000000	60.1
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	GCCGAGTACTCTACCATTGAG	TTTGAATTCCCAGCAATGGA	21	20	52.4	40.0	57.6	55.5	20.3	2.3	3.867846	4345	5278	955-995	1.000000	59.3
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	GCCGAGTACTCTACCATTGAG	TGAATTCCCAGCAATGGATACT	21	22	52.4	40.9	57.6	57.5	20.3	2.3	3.881967	4345	5278	953-993	1.000000	59.2
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	CCGAGAAAGTCTACGGTTCG	AGAGAAGCGGCCATAAACTG	20	20	55.0	50.0	58.1	58.0	0.0	2.2	0.137371	10475	11000	1022-1099	1.000000	86.6
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	CCGAGAAAGTCTACGGTTCG	TTCATAGTTACTGTGCCCGC	20	20	55.0	50.0	58.1	58.0	0.0	4.2	0.126074	10475	11000	1158-1235	1.000000	86.4
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	AGCCTGACAAAAGGTGGTTT	AGAGAAGCGGCCATAAACTG	20	20	45.0	50.0	57.8	58.0	0.0	2.2	0.189017	10475	11000	1499-1576	1.000000	85.9
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	AGCCTGACAAAAGGTGGTTT	TTCATAGTTACTGTGCCCGC	20	20	45.0	50.0	57.8	58.0	0.0	4.2	0.177721	10475	11000	1635-1712	1.000000	85.8
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	CCGAGAAAGTCTACGGTTCG	TTTGGGTTGTTCGGTCAAGT	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.239308	10475	11000	950-1027	0.750000	75.3
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	18218	18525	377-404	1.000000	70.1
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	18218	18525	380-407	1.000000	69.9
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	18218	18525	383-410	1.000000	69.4
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	18218	18525	386-413	1.000000	69.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	18218	18525	381-408	1.000000	67.6
atpA-atpF_p1	atpA-atpF	1	CCGAGATTCCGAAGCTGAAA	AGTCGAAACGGAAGCAGATC	20	20	50.0	50.0	58.0	58.0	3.0	0.0	0.012476	20511	20595	759-763	1.000000	82.2
atpA-atpF_p2	atpA-atpF	2	CTGACCACGCCCTATAGGTA	CGGTTTCGAGTGAATGGCTA	20	20	55.0	50.0	58.0	58.0	17.7	0.0	0.013462	20511	20595	848-852	1.000000	82.1
atpA-atpF_p3	atpA-atpF	3	CTGACCACGCCCTATAGGTA	AGTCGAAACGGAAGCAGATC	20	20	55.0	50.0	58.0	58.0	17.7	0.0	0.011210	20511	20595	867-871	1.000000	82.0
atpA-atpF_p4	atpA-atpF	4	CCGAGATTCCGAAGCTGAAA	ATACCGTGCGAAATCAACGA	20	20	50.0	45.0	58.0	58.0	3.0	7.1	0.011097	20511	20595	1207-1210	0.500000	61.2
atpA-atpF_p5	atpA-atpF	5	CTGACCACGCCCTATAGGTA	ATACCGTGCGAAATCAACGA	20	20	55.0	45.0	58.0	58.0	17.7	7.1	0.009831	20511	20595	1315-1318	0.500000	61.1
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	20	20	45.0	50.0	58.0	57.3	1.4	14.7	0.774530	22211	23279	1120-1142	1.000000	80.8
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	20	20	45.0	45.0	58.0	57.2	1.4	0.0	0.808551	22211	23279	1157-1179	1.000000	80.3
atpH-atpI_p3	atpH-atpI	3	GTCCAATAGAAGCAAGCCCA	CCGCGGCTTATATAGGTGAA	20	20	50.0	50.0	57.9	57.3	0.0	14.7	0.864914	22211	23279	1158-1180	1.000000	79.9
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	CCGCGGCTTATATAGGTGAA	20	20	45.0	50.0	58.2	57.3	1.4	14.7	0.920069	22211	23279	1121-1143	1.000000	79.6
atpH-atpI_p5	atpH-atpI	5	GTCCAATAGAAGCAAGCCCA	ACAAGCGGTATTCAAGCTCT	20	20	50.0	45.0	57.9	57.2	0.0	0.0	0.898934	22211	23279	1195-1217	1.000000	79.4
trnS-GGA-psbI_p1	trnS-GGA-psbI	1	GGCTTTTTGTTTACCGAGGG	CGTAATCCAGGACGTGAAGA	20	20	50.0	50.0	57.3	57.1	0.0	0.0	1.651075	53833	54024	250-256	1.000000	72.8
trnS-GGA-psbI_p2	trnS-GGA-psbI	2	TTCGAATCCCTCTCTTTCCG	CGTAATCCAGGACGTGAAGA	20	20	50.0	50.0	57.0	57.1	0.0	0.0	1.895021	53833	54024	230-236	1.000000	71.3
trnS-GGA-psbI_p3	trnS-GGA-psbI	3	GGCTTTTTGTTTACCGAGGG	CGTAATCCAGGACGTGAAGAA	20	21	50.0	47.6	57.3	57.8	0.0	0.0	1.943926	53833	54024	250-256	1.000000	70.4
trnS-GGA-psbI_p4	trnS-GGA-psbI	4	GGGTTCGAATCCCTCTCTTT	CGTAATCCAGGACGTGAAGA	20	20	50.0	50.0	56.9	57.1	17.2	0.0	2.016050	53833	54024	233-239	1.000000	70.3
trnS-GGA-psbI_p5	trnS-GGA-psbI	5	GGTTCGAATCCCTCTCTTTCC	CGTAATCCAGGACGTGAAGA	21	20	52.4	50.0	58.1	57.1	0.0	0.0	2.025861	53833	54024	232-238	1.000000	70.2
psbI-psbK_p1	psbI-psbK	1	TCTTCACGTCCTGGATTACG	CTTTTGTTTGGCAAGCTGCT	20	20	50.0	45.0	57.1	58.1	0.0	15.2	0.989464	54136	54624	619-631	1.000000	79.4
psbI-psbK_p2	psbI-psbK	2	TCTTCACGTCCTGGATTACG	GTTTGGCAAGCTGCTGTAAG	20	20	50.0	50.0	57.1	57.9	0.0	1.4	0.989589	54136	54624	614-626	1.000000	79.4
psbI-psbK_p3	psbI-psbK	3	TCTTCACGTCCTGGATTACG	GCGAAATTACCCGAAGCCTA	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.989464	54136	54624	701-713	1.000000	78.5
psbI-psbK_p4	psbI-psbK	4	TCTTCACGTCCTGGATTACG	TGGCAAGCTGCTGTAAGTTT	20	20	50.0	45.0	57.1	58.3	0.0	1.4	1.242299	54136	54624	611-623	1.000000	77.4
psbI-psbK_p5	psbI-psbK	5	TCTTCACGTCCTGGATTACG	TTGGCAAGCTGCTGTAAGTT	20	20	50.0	45.0	57.1	58.3	0.0	1.4	1.242299	54136	54624	612-624	1.000000	77.4
rps11-rpl36_p1	rps11-rpl36	1	ATGGTGTACCCCTTCGTGTA	GAAAGAATCGGCGGAGAAGT	20	20	50.0	50.0	58.1	58.0	0.0	7.2	0.070163	78822	79110	1000-1019	1.000000	86.6
rps11-rpl36_p2	rps11-rpl36	2	GAGACAGAATAAAGCGCCCA	GAAAGAATCGGCGGAGAAGT	20	20	50.0	50.0	58.0	58.0	2.3	7.2	0.035716	78822	79110	1411-1411	0.250000	49.7
rps11-rpl36_p3	rps11-rpl36	3	TGGAGACAGAATAAAGCGCC	GAAAGAATCGGCGGAGAAGT	20	20	50.0	50.0	58.0	58.0	2.3	7.2	0.035716	78822	79110	1413-1413	0.250000	49.7
rps11-rpl36_p4	rps11-rpl36	4	GAGACAGAATAAAGCGCCCA	GGGCCTCGACTCGTAAAATT	20	20	50.0	50.0	58.0	58.0	2.3	0.8	0.044123	78822	79110	993-993	0.250000	49.6
rps11-rpl36_p5	rps11-rpl36	5	TGGAGACAGAATAAAGCGCC	GGGCCTCGACTCGTAAAATT	20	20	50.0	50.0	58.0	58.0	2.3	0.8	0.044123	78822	79110	995-995	0.250000	49.6
rps3-rps19_p1	rps3-rps19	1	ACGTCCGGCAATTTGTACTT	GCGCCGGAGTAAAAAGTAGA	20	20	45.0	50.0	58.1	58.0	0.0	5.0	0.113543	83091	83415	1200-1246	1.000000	86.8
rps3-rps19_p2	rps3-rps19	2	GAAATGTTGAGTTTGCGGGG	GCGCCGGAGTAAAAAGTAGA	20	20	50.0	50.0	57.9	58.0	0.0	5.0	0.065003	83091	83415	1037-1083	0.750000	76.8
rps3-rps19_p3	rps3-rps19	3	GAAATGTTGAGTTTGCGGGG	GATTGGCCATACCGTTGCTA	20	20	50.0	50.0	57.9	58.0	0.0	6.1	0.102780	83091	83415	792-811	0.750000	76.6
rps3-rps19_p4	rps3-rps19	4	GAAATGTTGAGTTTGCGGGG	TTGGCCATACCGTTGCTATC	20	20	50.0	50.0	57.9	58.0	0.0	6.1	0.102780	83091	83415	790-809	0.750000	76.6
rps3-rps19_p5	rps3-rps19	5	AACCCTCCCTTCTCTGATCC	GCGCCGGAGTAAAAAGTAGA	20	20	55.0	50.0	58.1	58.0	0.0	5.0	0.114007	83091	83415	1254-1297	0.750000	75.7
ycf1_p1	ycf1	1	GGAACCGTCCAATGCGATAT	CCACGACAAGGTCCATTCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.230171	109925	110044	394-403	1.000000	87.2
ycf1_p2	ycf1	2	GGAACCGTCCAATGCGATAT	GGCTTTTCTGGAACGAATGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.138435	109925	110044	878-893	1.000000	86.7
ycf1_p3	ycf1	3	GTGGGAAACGGCAGAAACTA	GGCTTTTCTGGAACGAATGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.130501	109925	110044	1000-1006	0.500000	61.7
ycf1_p4	ycf1	4	GTGGGAAACGGCAGAAACTA	CCACGACAAGGTCCATTCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.222237	109925	110044	516-522	0.500000	61.0
ycf1_p5	ycf1	5	GTGTTTTTCGTACCCCTCCA	GGCTTTTCTGGAACGAATGC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.276654	109925	110044	769-775	0.500000	60.8
ndhG-ndhE_p1	ndhG-ndhE	1	GTGCAATTGTTGTGGCTCG	TTTCGGCTCGTGATCAATCC	19	20	52.6	50.0	58.2	58.3	7.4	0.0	1.547917	119763	119978	319-346	1.000000	73.1
ndhG-ndhE_p2	ndhG-ndhE	2	GGTGCAATTGTTGTGGCTC	TTTCGGCTCGTGATCAATCC	19	20	52.6	50.0	57.8	58.3	7.4	0.0	1.547776	119763	119978	320-347	1.000000	73.0
ndhG-ndhE_p3	ndhG-ndhE	3	GGTGCAATTGTTGTGGCTC	CGGCTCGTGATCAATCCATA	19	20	52.6	50.0	57.8	57.6	7.4	0.0	1.638632	119763	119978	317-344	1.000000	72.4
ndhG-ndhE_p4	ndhG-ndhE	4	GTGCAATTGTTGTGGCTCG	CGGCTCGTGATCAATCCATA	19	20	52.6	50.0	58.2	57.6	7.4	0.0	1.638773	119763	119978	316-343	1.000000	72.4
ndhG-ndhE_p5	ndhG-ndhE	5	GGTGCAATTGTTGTGGCTC	AACCATATTTCGGCTCGTGA	19	20	52.6	45.0	57.8	57.3	7.4	0.0	1.883765	119763	119978	327-354	1.000000	70.3
rpl32-ndhF_p1	rpl32-ndhF	1	AAATAAAATGCCATGCCGCC	GTGGGAAAAAGTAGGAGCCC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.212313	124326	124986	1315-1358	1.000000	86.0
rpl32-ndhF_p2	rpl32-ndhF	2	AAATAAAATGCCATGCCGCC	TTTGCTGCAATAGGACGTGT	20	20	45.0	45.0	58.0	58.1	0.0	16.8	0.156931	124326	124986	1741-1784	1.000000	85.0
rpl32-ndhF_p3	rpl32-ndhF	3	AAATAAAATGCCATGCCGCC	TCCCGAAATTCGAAACTCCC	20	20	45.0	50.0	58.0	57.9	0.0	17.7	0.151344	124326	124986	1843-1886	1.000000	84.8
rpl32-ndhF_p4	rpl32-ndhF	4	TCCTAAGAGCAGCGTGTCTA	TCCCGAAATTCGAAACTCCC	20	20	50.0	50.0	57.9	57.9	0.0	17.7	0.227814	124326	124986	1789-1832	1.000000	84.4
rpl32-ndhF_p5	rpl32-ndhF	5	AAATAAAATGCCATGCCGCC	CCCGAAATTCGAAACTCCCA	20	20	45.0	50.0	58.0	58.2	0.0	17.7	0.231733	124326	124986	1874-1874	0.250000	48.1