primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	ATTTTCCGGATGGGTTGCC	TCGATCATATCTACATATCATATTTTC	19	27	52.6	25.9	58.4	53.4	4.1	0.0	13.050118	1397	4033	2684-2692	1.000000	41.8
trnK-UUU_p2	trnK-UUU	2	ATTTTCCGGATGGGTTGCC	CGATCATATCTACATATCATATTTTC	19	26	52.6	26.9	58.4	52.1	4.1	0.0	13.356018	1397	4033	2683-2691	1.000000	41.8
trnK-UUU_p3	trnK-UUU	3	TTTTCCGGATGGGTTGCC	TCGATCATATCTACATATCATATTTTC	18	27	55.6	25.9	57.9	53.4	4.1	0.0	13.746210	1397	4033	2683-2691	1.000000	41.8
trnK-UUU_p4	trnK-UUU	4	TTTTCCGGATGGGTTGCC	CGATCATATCTACATATCATATTTTC	18	26	55.6	26.9	57.9	52.1	4.1	0.0	14.052110	1397	4033	2682-2690	1.000000	41.8
trnK-UUU_p5	trnK-UUU	5	ATTTTCCGGATGGGTTGCCC	TCGATCATATCTACATATCATATTTTC	20	27	55.0	25.9	61.0	53.4	4.1	0.0	14.616378	1397	4033	2684-2692	1.000000	41.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	5627	6712	1017-1177	1.000000	85.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	5627	6712	1015-1175	1.000000	85.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5627	6712	1023-1183	1.000000	85.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5627	6712	1024-1184	1.000000	85.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5627	6712	999-1159	1.000000	84.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	7984	8802	884-918	1.000000	56.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	TCGAACCCTCGGTACCAATA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.9	55.5	13.5	0.0	3.678199	7984	8802	917-951	1.000000	55.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	7984	8802	887-921	1.000000	55.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	7984	8802	886-920	1.000000	55.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GTCTTAAGCGATCGAAGGGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.1	55.5	21.6	0.0	3.681616	7984	8802	953-987	1.000000	55.6
atpF-atpH_p1	atpF-atpH	1	TTAAACCCGAAACTCCCAGC	CGGACTAGTCGTGGCATTAG	20	20	50.0	55.0	58.1	57.9	0.0	16.6	0.220871	12871	13048	295-603	1.000000	79.2
atpF-atpH_p2	atpF-atpH	2	TTAAACCCGAAACTCCCAGC	GGACTAGTCGTGGCATTAGC	20	20	50.0	55.0	58.1	58.1	0.0	16.6	0.245528	12871	13048	294-602	1.000000	79.0
atpF-atpH_p3	atpF-atpH	3	TTAAACCCGAAACTCCCAGC	GCGGAGGGTAAAATACGAGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.237784	12871	13048	360-668	1.000000	78.9
atpF-atpH_p4	atpF-atpH	4	TTAAACCCGAAACTCCCAGC	AGTCGTGGCATTAGCACTTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.284759	12871	13048	289-597	1.000000	78.7
atpF-atpH_p5	atpF-atpH	5	TTAAACCCGAAACTCCCAGC	ACTAGTCGTGGCATTAGCAC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.395007	12871	13048	292-600	1.000000	77.8
atpH-atpI_p1	atpH-atpI	1	TCCGATAGAAGCAAGCCCTA	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.9	58.5	0.0	0.0	0.599863	13295	13855	679-686	1.000000	77.7
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.1	58.5	0.0	0.0	0.621503	13295	13855	640-647	1.000000	77.6
atpH-atpI_p3	atpH-atpI	3	TCCGATAGAAGCAAGCCCTA	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	57.9	57.4	0.0	21.3	0.669200	13295	13855	652-659	1.000000	77.2
atpH-atpI_p4	atpH-atpI	4	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.1	58.5	0.0	0.0	0.655741	13295	13855	725-732	1.000000	77.1
atpH-atpI_p5	atpH-atpI	5	GATAGAAGCAAGCCCTACGG	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	57.8	58.5	0.0	0.0	0.674066	13295	13855	676-683	1.000000	77.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCCGTAGC	TGCCATATGTCTCACTGGGA	20	20	50.0	50.0	58.3	58.1	0.0	1.2	0.394936	28596	29392	820-937	1.000000	81.6
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCCGTAGC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	58.3	58.5	0.0	5.4	0.750478	28596	29392	794-911	1.000000	78.7
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCCGTAGC	ATGCCATATGTCTCACTGGG	20	20	50.0	50.0	58.3	57.1	0.0	1.2	1.218227	28596	29392	821-938	1.000000	75.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TAGAATGAACAGTGCCGTAGC	TGCCATATGTCTCACTGGGA	21	20	47.6	50.0	58.1	58.1	0.0	1.2	1.233196	28596	29392	821-938	1.000000	74.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ACTAGAATGAACAGTGCCGT	TGCCATATGTCTCACTGGGA	20	20	45.0	50.0	56.9	58.1	0.0	1.2	1.252324	28596	29392	823-940	1.000000	74.7
trnS-UGA-lhbA_p1	trnS-UGA-lhbA	1	GTTTTCAAGACCGGGGCTAT	ACCAACCATCAGAAGAAGCA	20	20	50.0	45.0	57.9	57.0	0.8	0.0	1.081679	34429	34776	489-489	1.000000	73.0
trnS-UGA-lhbA_p2	trnS-UGA-lhbA	2	AGCAAAAGGAGAGAGAGGGA	ACCAACCATCAGAAGAAGCA	20	20	50.0	45.0	57.7	57.0	0.0	0.0	1.291408	34429	34776	547-547	1.000000	71.1
trnS-UGA-lhbA_p3	trnS-UGA-lhbA	3	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGAAGAAGCA	20	21	50.0	47.6	57.9	58.2	0.8	0.0	1.341143	34429	34776	490-490	1.000000	70.9
trnS-UGA-lhbA_p4	trnS-UGA-lhbA	4	GTTTTCAAGACCGGGGCTAT	TGACCAACCATCAGAAGAAGC	20	21	50.0	47.6	57.9	58.2	0.8	0.0	1.341143	34429	34776	491-491	1.000000	70.9
trnS-UGA-lhbA_p5	trnS-UGA-lhbA	5	GTTTTCAAGACCGGGGCTAT	ACCAACCATCAGAAGAAGCAA	20	21	50.0	42.9	57.9	57.8	0.8	0.0	1.352465	34429	34776	489-489	1.000000	70.8
trnL-UAA_p1	trnL-UAA	1	AGTATTCCAAATCGCGCTGT	GTGCCAGGAACCAGATTTGA	20	20	45.0	50.0	58.0	58.1	5.6	0.0	0.114476	46186	46808	1325-1349	1.000000	81.5
trnL-UAA_p2	trnL-UAA	2	AGTATTCCAAATCGCGCTGT	ATGTATCCCGACCCGTCTAG	20	20	45.0	55.0	58.0	58.1	5.6	0.0	0.117186	46186	46808	1383-1407	1.000000	81.4
trnL-UAA_p3	trnL-UAA	3	AGTATTCCAAATCGCGCTGT	CGTGCATCATCCTAGTGGAG	20	20	45.0	55.0	58.0	57.9	5.6	0.0	0.170133	46186	46808	1244-1268	1.000000	81.2
trnL-UAA_p4	trnL-UAA	4	AGTATTCCAAATCGCGCTGT	GAGCTATCCCGACCATTTCC	20	20	45.0	55.0	58.0	57.8	5.6	0.0	0.195516	46186	46808	1264-1288	1.000000	80.9
trnL-UAA_p5	trnL-UAA	5	AGACATCCCTCCAGCTTCAT	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199748	46186	46808	1397-1421	1.000000	80.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CTCCACTAGGATGATGCACG	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.274728	47263	47965	874-879	1.000000	79.4
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	47263	47965	854-859	1.000000	79.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	47263	47965	851-856	1.000000	78.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	47263	47965	850-855	1.000000	78.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTCCACTAGGATGATGCACG	ATAGGCTGGCCCTTACGTAA	20	20	55.0	50.0	57.9	58.2	0.0	7.9	0.348637	47263	47965	864-869	1.000000	78.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391484	49645	50186	664-674	1.000000	79.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGTCCTTTACTAATCGGGGC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.448692	49645	50186	678-688	1.000000	78.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.617224	49645	50186	666-676	1.000000	77.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.617342	49645	50186	667-677	1.000000	77.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CGGGGCTAAAACTCCAGAAA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.668261	49645	50186	746-756	1.000000	76.6
accD-psaI_p1	accD-psaI	1	AGTTCCACGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.049947	57422	58099	650-792	1.000000	84.4
accD-psaI_p2	accD-psaI	2	GTTCCACGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.049947	57422	58099	649-791	1.000000	84.4
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	57422	58099	646-788	1.000000	84.4
accD-psaI_p4	accD-psaI	4	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	57422	58099	647-789	1.000000	84.4
accD-psaI_p5	accD-psaI	5	GTTCCACGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.126481	57422	58099	655-797	1.000000	83.7
rpl16-rps3_p1	rpl16-rps3	1	AACGAGTCACACACTAAGCA	TTTGGATATTTGTAGACGAGT	20	21	45.0	33.3	57.1	52.3	0.0	0.0	7.567542	80994	81162	211-213	1.000000	44.2
rpl16-rps3_p2	rpl16-rps3	2	ACGAGTCACACACTAAGCAT	TTTGGATATTTGTAGACGAGT	20	21	45.0	33.3	56.9	52.3	0.0	0.0	7.793829	80994	81162	210-212	1.000000	44.2
rpl16-rps3_p3	rpl16-rps3	3	AACGAGTCACACACTAAGCAT	TTTGGATATTTGTAGACGAGT	21	21	42.9	33.3	57.6	52.3	0.0	0.0	8.071383	80994	81162	211-213	1.000000	44.2
rpl16-rps3_p4	rpl16-rps3	4	AACGAGTCACACACTAAGCA	ATTTGGATATTTGTAGACGAGT	20	22	45.0	31.8	57.1	53.0	0.0	0.0	7.883707	80994	81162	212-214	1.000000	44.1
rpl16-rps3_p5	rpl16-rps3	5	AACGAGTCACACACTAAGCA	AATTTGGATATTTGTAGACGAGT	20	23	45.0	30.4	57.1	53.8	0.0	0.0	8.042198	80994	81162	213-215	1.000000	44.1
rps3_p1	rps3	1	TGACAAGACAAATAAAGGTCCAC	CCACCTGTCATATAACAATTGT	23	22	39.1	36.4	57.0	54.2	0.0	4.9	9.863900	81163	81816	772-800	1.000000	43.3
rps3_p2	rps3	2	GACAAGACAAATAAAGGTCCACT	CCACCTGTCATATAACAATTGT	23	22	39.1	36.4	56.7	54.2	0.0	4.9	10.126092	81163	81816	771-799	1.000000	43.3
rps3_p3	rps3	3	TGACAAGACAAATAAAGGTCCACT	CCACCTGTCATATAACAATTGT	24	22	37.5	36.4	58.3	54.2	0.0	4.9	10.153010	81163	81816	772-800	1.000000	43.3
rps3_p4	rps3	4	TGACAAGACAAATAAAGGTCCA	CCACCTGTCATATAACAATTGT	22	22	36.4	36.4	55.6	54.2	0.0	4.9	10.275847	81163	81816	772-800	1.000000	43.3
rps3_p5	rps3	5	ACAAGACAAATAAAGGTCCACT	CCACCTGTCATATAACAATTGT	22	22	36.4	36.4	55.6	54.2	0.0	4.9	10.289706	81163	81816	770-798	1.000000	43.3
ndhE-psaC_p1	ndhE-psaC	1	GGAAGCAGCTATTGGGCTAT	CTTAGCTTTACACCCGTCCC	20	20	50.0	55.0	57.4	58.0	0.0	0.0	0.595752	119669	120053	540-587	1.000000	80.6
ndhE-psaC_p2	ndhE-psaC	2	GGAAGCAGCTATTGGGCTAT	TTACACCCGTCCCAAGGTAT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.620354	119669	120053	533-580	1.000000	80.5
ndhE-psaC_p3	ndhE-psaC	3	GGAAGCAGCTATTGGGCTAT	TGCTTAGCTTTACACCCGTC	20	20	50.0	50.0	57.4	57.9	0.0	5.6	0.664754	119669	120053	542-589	1.000000	80.1
ndhE-psaC_p4	ndhE-psaC	4	GGAAGCAGCTATTGGGCTAT	CAAAACATCCGTTGGGCAAG	20	20	50.0	50.0	57.4	58.2	0.0	0.0	0.793761	119669	120053	507-554	1.000000	79.1
ndhE-psaC_p5	ndhE-psaC	5	TGTTATAGCGATTGCAGCCG	CTTAGCTTTACACCCGTCCC	20	20	50.0	55.0	58.8	58.0	7.9	0.0	0.802946	119669	120053	561-608	1.000000	78.9
ndhD_p1	ndhD	1	GGGACGGGTGTAAAGCTAAG	GCATTCTTTCCGGAGCAGTA	20	20	55.0	50.0	58.0	58.0	0.0	3.3	0.044123	120418	121923	2244-2278	1.000000	80.6
ndhD_p2	ndhD	2	ATACCTTGGGACGGGTGTAA	GCATTCTTTCCGGAGCAGTA	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.068725	120418	121923	2251-2285	1.000000	80.4
ndhD_p3	ndhD	3	GGGACGGGTGTAAAGCTAAG	TTTCTTTGGCGAGCTCCAAT	20	20	55.0	45.0	58.0	58.1	0.0	8.5	0.110248	120418	121923	2402-2436	1.000000	80.1
ndhD_p4	ndhD	4	GACGGGTGTAAAGCTAAGCA	GCATTCTTTCCGGAGCAGTA	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.113125	120418	121923	2242-2276	1.000000	80.1
ndhD_p5	ndhD	5	ATACCTTGGGACGGGTGTAA	TTTCTTTGGCGAGCTCCAAT	20	20	50.0	45.0	58.0	58.1	0.0	8.5	0.134849	120418	121923	2409-2443	1.000000	79.9
ndhD-ccsA_p1	ndhD-ccsA	1	ATCCGGATTTCGTTCTCTCC	GCGCTTGTGGCTTCTATAGG	20	20	50.0	55.0	57.1	58.8	12.4	0.0	1.671370	121924	122128	387-421	1.000000	69.2
ndhD-ccsA_p2	ndhD-ccsA	2	ATCCGGATTTCGTTCTCTCC	GGCGCTTGTGGCTTCTATAG	20	20	50.0	55.0	57.1	58.8	12.4	7.6	1.671370	121924	122128	388-422	1.000000	69.2
ndhD-ccsA_p3	ndhD-ccsA	3	ATCCGGATTTCGTTCTCTCC	CGCTTGTGGCTTCTATAGGA	20	20	50.0	50.0	57.1	57.1	12.4	0.0	1.783592	121924	122128	386-420	1.000000	68.3
ndhD-ccsA_p4	ndhD-ccsA	4	CCGGATTTCGTTCTCTCCCT	GCGCTTGTGGCTTCTATAGG	20	20	55.0	55.0	59.2	58.8	0.0	0.0	1.953826	121924	122128	385-419	1.000000	67.0
ndhD-ccsA_p5	ndhD-ccsA	5	CCGGATTTCGTTCTCTCCCT	GGCGCTTGTGGCTTCTATAG	20	20	55.0	55.0	59.2	58.8	0.0	7.6	1.953826	121924	122128	386-420	1.000000	67.0
rpl32-ndhF_p1	rpl32-ndhF	1	CTTGCTTGTTTGTCGCACAA	CAAGAGGTCGTGTGAACCAA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.145139	124015	124972	1549-1662	1.000000	84.7
rpl32-ndhF_p2	rpl32-ndhF	2	CTTGCTTGTTTGTCGCACAA	AGGGGAAATTGTGCGGATTT	20	20	45.0	45.0	58.1	58.1	0.0	0.0	0.156052	124015	124972	1775-1888	1.000000	84.4
rpl32-ndhF_p3	rpl32-ndhF	3	CGCGGTAGGGTTCAGTTTTA	CAAGAGGTCGTGTGAACCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.135062	124015	124972	2081-2180	0.666667	69.9
rpl32-ndhF_p4	rpl32-ndhF	4	CGCGGTAGGGTTCAGTTTTA	AAGGGGAAATTGTGCGGATT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.145975	124015	124972	2308-2407	0.666667	69.4
rpl32-ndhF_p5	rpl32-ndhF	5	CGCGGTAGGGTTCAGTTTTA	AGGGGAAATTGTGCGGATTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.145975	124015	124972	2307-2406	0.666667	69.4