primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCCTTTCAGGATCAGTCGTG	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.091715	4382	5161	1190-1233	1.000000	91.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.091715	4382	5161	1189-1232	1.000000	91.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTTCAGGATCAGTCGTGG	ACGAATCAACCGTCGGTATG	20	20	55.0	50.0	58.0	58.1	0.0	19.2	0.098463	4382	5161	1627-1675	1.000000	90.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCCTTTCAGGATCAGTCGTG	AGAACAAGGAAACACCGCTT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.068518	4382	5161	1494-1542	0.875000	84.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	AGAACAAGGAAACACCGCTT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.068518	4382	5161	1493-1541	0.875000	84.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GATCGTTGCCCCTGAGAAAT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.100217	6298	7242	1747-1832	1.000000	91.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GATCGTTGCCCCTGAGAAAT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.120224	6298	7242	1163-1247	1.000000	91.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	ATACCGACGGTTGATTCGTG	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.1	57.9	27.1	0.0	0.138822	6298	7242	1911-1996	1.000000	90.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGCGGTGTTTCCTTGTTCT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.9	58.1	0.0	16.9	0.128883	6298	7242	1511-1546	0.875000	83.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGCGGTGTTTCCTTGTTCT	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.108877	6298	7242	2093-2131	0.875000	83.1
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8603	9353	831-849	1.000000	84.2
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8603	9353	813-831	1.000000	82.0
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	TTTCGTCCACTCAGCCATCT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	59.0	58.1	0.0	0.0	1.105487	8603	9353	830-848	1.000000	81.1
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	TCCACTCAGCCATCTCTCC	CCGGATTTGAACTGGGGAAA	19	20	57.9	50.0	58.1	58.1	0.0	0.0	1.174425	8603	9353	825-843	1.000000	80.5
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	ATTAGCAATCCGCCGCTTT	CCGGATTTGAACTGGGGAAA	19	20	47.4	50.0	58.2	58.1	0.0	0.0	1.288297	8603	9353	846-864	1.000000	79.6
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9435	10221	853-915	0.875000	84.8
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9435	10221	871-933	0.875000	82.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	ATCAACGGGCTATAATGGGC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.282501	9435	10221	1309-1379	0.500000	63.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	ATCAACGGGCTATAATGGGC	20	20	50.0	50.0	58.4	57.8	14.7	0.0	0.555864	9435	10221	1327-1397	0.500000	60.9
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GGCTATAATGGGCGGTAGTC	20	20	50.0	55.0	58.1	57.6	0.0	0.0	0.463853	9435	10221	1302-1372	0.375000	55.5
petN-psbM_p1	petN-psbM	1	GCGGCTAACTAGTTGTGGAA	TTTGATTGGTCCGGGAATCC	20	20	50.0	50.0	57.9	57.9	18.6	0.0	0.226759	10312	10807	1460-1516	0.625000	71.1
petN-psbM_p2	petN-psbM	2	TCCGTTCTAGCGTCATTGTG	GTCCCGACGGATCCAATAAC	20	20	50.0	55.0	58.0	58.1	0.0	17.5	0.147230	10312	10807	1502-1519	0.375000	58.5
petN-psbM_p3	petN-psbM	3	TCCGTTCTAGCGTCATTGTG	TTTGATTGGTCCGGGAATCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.147011	10312	10807	1138-1152	0.375000	57.9
petN-psbM_p4	petN-psbM	4	TCCGTTCTAGCGTCATTGTG	AGTCCCGACGGATCCAATAA	20	20	50.0	50.0	58.0	58.2	0.0	17.5	0.224843	10312	10807	1503-1520	0.375000	57.9
petN-psbM_p5	petN-psbM	5	TCCGTTCTAGCGTCATTGTG	CCGCGACTCATCTTTCTCAT	20	20	50.0	50.0	58.0	57.8	0.0	8.2	0.225108	10312	10807	1356-1370	0.250000	50.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AAATGCAAGAATATTTACTTCCA	CTGTCAAGGCGGACGTTG	23	18	26.1	61.1	52.7	58.8	0.0	7.6	11.095131	10913	11513	654-750	1.000000	52.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATGCAAGAATATTTACTTCCAT	CTGTCAAGGCGGACGTTG	23	18	26.1	61.1	52.4	58.8	0.0	7.6	11.312974	10913	11513	653-749	1.000000	52.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAATGCAAGAATATTTACTTCCAT	CTGTCAAGGCGGACGTTG	24	18	25.0	61.1	53.3	58.8	0.0	7.6	11.476492	10913	11513	654-750	1.000000	52.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TAAATGCAAGAATATTTACTTCCA	CTGTCAAGGCGGACGTTG	24	18	25.0	61.1	52.7	58.8	0.0	7.6	12.015581	10913	11513	655-751	1.000000	52.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAATGCAAGAATATTTACTTCCA	CCTGTCAAGGCGGACGTT	23	18	26.1	61.1	52.7	60.0	0.0	7.6	12.308674	10913	11513	655-751	1.000000	52.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GAATCAAACCGAGAGACCCC	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	58.0	58.0	0.0	5.0	0.029297	30213	31218	1374-1600	1.000000	91.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GAATCAAACCGAGAGACCCC	TGGACCAGAGAATGCGATTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.054008	30213	31218	1740-1966	1.000000	90.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GAATCAAACCGAGAGACCCC	AAACTAGGCGCAAAGAACCA	20	20	55.0	45.0	58.0	58.0	0.0	10.8	0.049929	30213	31218	1811-2037	1.000000	90.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GAATCAAACCGAGAGACCCC	AACTAGGCGCAAAGAACCAA	20	20	55.0	45.0	58.0	58.0	0.0	10.8	0.049929	30213	31218	1810-2036	1.000000	90.7
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GAATCAAACCGAGAGACCCC	ACCAAAACTAGGCGCAAAGA	20	20	55.0	45.0	58.0	58.0	0.0	10.8	0.049929	30213	31218	1815-2041	1.000000	90.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	AACATAGCATCTGAGGCTGC	TTGGGTATGGCTCCGACTAT	20	20	50.0	50.0	58.0	57.9	25.4	0.0	0.132558	42842	43757	1830-1953	1.000000	90.8
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	AACATAGCATCTGAGGCTGC	TGGGTATGGCTCCGACTATT	20	20	50.0	50.0	58.0	57.9	25.4	0.0	0.132558	42842	43757	1829-1952	1.000000	90.7
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCAACGCTTCCCAATTTCCA	TTGGGTATGGCTCCGACTAT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108817	42842	43757	2108-2231	0.875000	84.9
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TCAACGCTTCCCAATTTCCA	TGGGTATGGCTCCGACTATT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.108817	42842	43757	2107-2230	0.875000	84.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TCAACGCTTCCCAATTTCCA	TGAAATGCGCCTGGATAACA	20	20	45.0	45.0	58.0	57.9	0.0	4.2	0.132998	42842	43757	2139-2262	0.875000	84.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	45190	45756	602-653	1.000000	89.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	45190	45756	634-685	1.000000	88.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	45190	45756	596-647	1.000000	87.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	45190	45756	628-679	1.000000	86.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	45190	45756	601-652	1.000000	86.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGCATTCCATTGCTGTCAT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.196601	46696	47321	1011-1051	1.000000	89.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCCAATGCGCCTATGATGTA	20	20	50.0	50.0	58.1	57.8	0.0	4.2	0.235374	46696	47321	959-993	0.875000	83.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATAAAAAGTCGCGGCTCACT	20	20	50.0	45.0	58.1	57.9	0.0	16.4	0.146658	46696	47321	1206-1231	0.500000	63.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AGAGACTCGATGGAAGCTGT	ATAAAAAGTCGCGGCTCACT	20	20	50.0	45.0	58.2	57.9	0.0	16.4	0.248756	46696	47321	1919-1942	0.500000	63.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATTATCTCCGCTCCCAATGC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.243023	46696	47321	993-993	0.125000	43.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTTGCTACTCGGAGCTA	ATGAAAGAGCCCAATGCACA	20	20	50.0	45.0	57.9	58.1	4.6	0.0	0.211836	49000	49966	529-1411	1.000000	90.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCGTCGCCTATTGGTTCTAT	ATGAAAGAGCCCAATGCACA	20	20	50.0	45.0	57.8	58.1	5.8	0.0	0.222910	49000	49966	579-1461	1.000000	90.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GTCTAGGACTCGACCTTGGT	ATGAAAGAGCCCAATGCACA	20	20	55.0	45.0	58.2	58.1	0.0	0.0	0.235710	49000	49966	1043-1925	1.000000	89.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CGTGAGCCTATTAGGGAAGC	ATGAAAGAGCCCAATGCACA	20	20	55.0	45.0	57.8	58.1	11.7	0.0	0.228856	49000	49966	1668-1878	0.875000	83.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTTGCTACTCGGAGCTA	GACAAGGGTTCCATCCGTTT	20	20	50.0	50.0	57.9	58.1	4.6	0.0	0.233686	49000	49966	1579-1790	0.500000	63.9
rbcL-accD_p1	rbcL-accD	1	AGGCTACCAAATGGAGTCCT	CAATAGGACCAAGACTGCCC	20	20	50.0	55.0	58.0	58.0	11.3	0.0	0.071535	55200	55708	724-749	0.500000	68.8
rbcL-accD_p2	rbcL-accD	2	GGCTACCAAATGGAGTCCTG	CAATAGGACCAAGACTGCCC	20	20	55.0	55.0	58.0	58.0	11.3	0.0	0.088245	55200	55708	723-748	0.500000	68.7
rbcL-accD_p3	rbcL-accD	3	CTAACAGGGGGATTCACTGC	CAATAGGACCAAGACTGCCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.088245	55200	55708	1256-1281	0.500000	66.5
rbcL-accD_p4	rbcL-accD	4	TTGAATGCTACTGCGGGTAC	CAATAGGACCAAGACTGCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.062665	55200	55708	1346-1371	0.500000	66.2
rbcL-accD_p5	rbcL-accD	5	AATGCTACTGCGGGTACATG	CAATAGGACCAAGACTGCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.090564	55200	55708	1343-1368	0.500000	66.0
petA-psbJ_p1	petA-psbJ	1	CTGACGGGAGCAAGAGTAAC	TGGAGGGAAGTGGGGTAAAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.071162	59653	60429	1225-1297	1.000000	90.7
petA-psbJ_p2	petA-psbJ	2	CTGACGGGAGCAAGAGTAAC	TCCCTATTCATTGCGGGTTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.053665	59653	60429	1791-1863	1.000000	89.1
petA-psbJ_p3	petA-psbJ	3	CGGATTTGGTCAGGGAGATG	TCCCTATTCATTGCGGGTTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.092293	59653	60429	1571-1643	1.000000	89.1
petA-psbJ_p4	petA-psbJ	4	GCATCTGATGGACGTCAAGT	TGGAGGGAAGTGGGGTAAAT	20	20	50.0	50.0	58.0	57.9	18.9	0.0	0.084567	59653	60429	1118-1190	0.750000	79.4
petA-psbJ_p5	petA-psbJ	5	GCATCTGATGGACGTCAAGT	TCCCTATTCATTGCGGGTTG	20	20	50.0	50.0	58.0	57.9	18.9	0.0	0.067070	59653	60429	1684-1756	0.750000	78.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAATAACTAGTTATGGTAGGCA	20	22	50.0	31.8	57.9	52.3	6.4	25.2	7.747449	61221	62374	1281-1377	1.000000	52.0
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AAATAACTAGTTATGGTAGGCA	20	22	50.0	31.8	57.8	52.3	24.9	25.2	7.852458	61221	62374	1204-1300	1.000000	52.0
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAATAACTAGTTATGGTAGGCA	20	22	45.0	31.8	58.2	52.3	0.0	25.2	7.867136	61221	62374	1290-1386	1.000000	52.0
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AAATAACTAGTTATGGTAGGCA	20	22	50.0	31.8	58.2	52.3	0.0	25.2	7.880041	61221	62374	1295-1391	1.000000	52.0
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AAATAACTAGTTATGGTAGGCA	20	22	50.0	31.8	57.8	52.3	0.0	25.2	7.883466	61221	62374	1289-1385	1.000000	52.0
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	ATATCTGCCCTTCCCGAGAA	20	20	50.0	50.0	58.0	57.9	17.5	0.0	0.135568	107483	108588	1580-1664	0.750000	80.9
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	ATATCTGCCCTTCCCGAGAA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.146857	107483	108588	1578-1662	0.750000	80.8
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	ATATCTGCCCTTCCCGAGAA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.146857	107483	108588	1579-1663	0.750000	80.8
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	TATCTGCCCTTCCCGAGAAA	20	20	50.0	50.0	58.0	58.1	17.5	0.0	0.158285	107483	108588	1579-1663	0.750000	80.7
ndhF-rpl32_p5	ndhF-rpl32	5	TGGGTCGATCAAGTATCCGA	ATATCTGCCCTTCCCGAGAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.164663	107483	108588	1755-1839	0.750000	80.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	108754	109602	651-1045	1.000000	88.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	108754	109602	652-1046	1.000000	88.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	108754	109602	683-1062	0.875000	84.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	108754	109602	684-1063	0.875000	84.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGAAAGGATATTGGGCGG	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.453915	108754	109602	1191-1584	0.875000	83.4
ycf1_p1	ycf1	1	AAGGAAGGCGTTTTGAGAGG	AAGAAATCCTCATTCCCCGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.161033	122055	122174	600-600	0.250000	51.5
ycf1_p2	ycf1	2	AAGGAAGGCGTTTTGAGAGG	GAAATCCTCATTCCCCGCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.161033	122055	122174	598-598	0.250000	51.5
ycf1_p3	ycf1	3	AAGGAAGGCGTTTTGAGAGG	ACCTGATTTTCGTCGATCCG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.179870	122055	122174	678-678	0.250000	51.2
ycf1_p4	ycf1	4	AAGGAAGGCGTTTTGAGAGG	CCTGATTTTCGTCGATCCGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.179870	122055	122174	677-677	0.250000	51.2
ycf1_p5	ycf1	5	AAGGAAGGCGTTTTGAGAGG	ATCAAAGGATCTATGCGGGC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.293568	122055	122174	656-656	0.250000	50.2