primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	TTAGCAATCCGACGCCTTAG	AACTGAGAGACCCCTTAACT	20	20	50.0	45.0	57.8	55.1	0.0	0.0	3.154523	9417	11388	1999-2083	1.000000	60.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	TTAGCAATCCGACGCCTTAG	ACTGAGAGACCCCTTAACTT	20	20	50.0	45.0	57.8	55.1	0.0	0.0	3.154523	9417	11388	1998-2082	1.000000	60.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TTAGCAATCCGACGCCTTAG	ACTGAGAGACCCCTTAACTTTTG	20	23	50.0	43.5	57.8	58.0	0.0	0.0	3.244554	9417	11388	1998-2082	1.000000	59.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGGAGAGAGAGGGATTCGAA	AACTGAGAGACCCCTTAACT	20	20	55.0	45.0	58.3	55.1	0.0	0.0	3.254032	9417	11388	2048-2132	1.000000	59.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CGGAGAGAGAGGGATTCGAA	ACTGAGAGACCCCTTAACTT	20	20	55.0	45.0	58.3	55.1	0.0	0.0	3.254032	9417	11388	2047-2131	1.000000	59.4
atpH-atpI_p1	atpH-atpI	1	TACCTTCTACAGCTTGGCCT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	58.0	57.9	3.3	21.3	0.113177	16196	17427	1377-1494	1.000000	79.6
atpH-atpI_p2	atpH-atpI	2	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	20	20	50.0	45.0	58.0	57.9	3.3	0.0	0.126227	16196	17427	1404-1521	1.000000	79.5
atpH-atpI_p3	atpH-atpI	3	AACAGAAGCGGCAGAAATCA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.1	57.9	1.4	21.3	0.169579	16196	17427	1292-1409	1.000000	79.2
atpH-atpI_p4	atpH-atpI	4	AACAGAAGCGGCAGAAATCA	ATTTTTGCAACCTTAGCCGC	20	20	45.0	45.0	58.1	57.9	1.4	0.0	0.182629	16196	17427	1319-1436	1.000000	79.1
atpH-atpI_p5	atpH-atpI	5	CAGCAGCAATAACAGAAGCG	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.8	57.9	0.0	21.3	0.235461	16196	17427	1302-1419	1.000000	78.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.149342	35439	36178	879-879	1.000000	79.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.207439	35439	36178	858-858	1.000000	79.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TCCTTTCGTAGTACCCCCAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.229593	35439	36178	855-855	1.000000	79.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	35439	36178	843-843	1.000000	79.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	35439	36178	822-822	1.000000	78.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	36251	37496	1396-1473	1.000000	78.1
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	36251	37496	1399-1476	1.000000	77.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	22.4	0.0	0.340432	36251	37496	1415-1492	1.000000	77.9
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	36251	37496	1395-1472	1.000000	76.9
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.480767	36251	37496	1398-1475	1.000000	76.8
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TAGTCCTGGTAAAACCCCCA	ATTATGTGAGCCCGCTTAGC	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.552729	50635	51133	645-651	1.000000	79.3
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TAGTCCTGGTAAAACCCCCA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.6	57.9	0.0	0.0	0.569704	50635	51133	626-632	1.000000	79.3
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TAGTCCTGGTAAAACCCCCA	GATGGTCATCGGTTCGACTC	20	20	50.0	55.0	57.6	58.2	0.0	0.0	0.595514	50635	51133	595-601	1.000000	79.2
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAGTCCTGGTAAAACCCCCA	GTGATTATGTGAGCCCGCTT	20	20	50.0	50.0	57.6	58.3	0.0	0.0	0.768545	50635	51133	648-654	1.000000	77.5
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TAGTCCTGGTAAAACCCCCA	ATGTGATTATGTGAGCCCGC	20	20	50.0	50.0	57.6	58.4	0.0	0.0	0.839222	50635	51133	650-656	1.000000	77.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	6.3	0.0	0.231387	55655	57669	2150-2152	1.000000	79.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	55655	57669	2144-2146	1.000000	78.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTAACCGGGGCTAAAACTCC	AGCCCAATGCAACAAAATGC	20	20	55.0	45.0	58.0	58.5	6.3	0.0	0.508164	55655	57669	2232-2234	1.000000	77.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CTAACCGGGGCTAAAACTCC	GAAGGTCTACGGTTCGAGTC	20	20	55.0	55.0	58.0	57.5	6.3	0.0	0.575159	55655	57669	2149-2151	1.000000	76.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	5.2	0.0	0.613383	55655	57669	2226-2228	1.000000	76.2
accD-psaI_p1	accD-psaI	1	CACGGTTTCCTTCCCTTGAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	65263	66143	969-982	1.000000	83.8
accD-psaI_p2	accD-psaI	2	CACGGTTTCCTTCCCTTGAA	AGAAACCATTGCAATTGCCG	20	20	50.0	45.0	58.0	57.9	0.0	26.9	0.107507	65263	66143	974-987	1.000000	83.4
accD-psaI_p3	accD-psaI	3	TCAACTCCACGGTTTCCTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	0.0	26.9	0.276847	65263	66143	976-989	1.000000	82.0
accD-psaI_p4	accD-psaI	4	TCAACTCCACGGTTTCCTTC	AGAAACCATTGCAATTGCCG	20	20	50.0	45.0	57.8	57.9	0.0	26.9	0.333010	65263	66143	981-994	1.000000	81.6
accD-psaI_p5	accD-psaI	5	TTCAACTCCACGGTTTCCTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.3	58.0	0.0	26.9	0.748429	65263	66143	977-990	1.000000	78.3
petA-psbJ_p1	petA-psbJ	1	CGGGTCCAAGGTCTTTTGTT	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.357230	69692	70704	1148-1148	1.000000	78.3
petA-psbJ_p2	petA-psbJ	2	CGGGTCCAAGGTCTTTTGTT	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.357230	69692	70704	1146-1146	1.000000	78.3
petA-psbJ_p3	petA-psbJ	3	CGGGTCCAAGGTCTTTTGTT	TGGTATCCCTGTGATCGGTT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.438308	69692	70704	1197-1197	1.000000	77.6
petA-psbJ_p4	petA-psbJ	4	TACGGGTCCAAGGTCTTTTG	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.599163	69692	70704	1150-1150	1.000000	76.4
petA-psbJ_p5	petA-psbJ	5	TACGGGTCCAAGGTCTTTTG	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.599163	69692	70704	1148-1148	1.000000	76.4
psaJ-rpl33_p1	psaJ-rpl33	1	ATCGTTTATTCCCGGATGCC	ACCCGGGGATTCCTTATTGA	20	20	50.0	50.0	58.1	58.1	0.7	27.9	0.206143	74457	75069	694-741	1.000000	82.4
psaJ-rpl33_p2	psaJ-rpl33	2	GTCTTTAGCAGGCCTGTTGA	ACCCGGGGATTCCTTATTGA	20	20	50.0	50.0	57.8	58.1	32.8	27.9	0.283357	74457	75069	723-770	1.000000	81.7
psaJ-rpl33_p3	psaJ-rpl33	3	ATCGTTTATTCCCGGATGCC	AGAAATACCCGGGGATTCCT	20	20	50.0	50.0	58.1	57.8	0.7	31.7	0.315717	74457	75069	700-747	1.000000	81.5
psaJ-rpl33_p4	psaJ-rpl33	4	ATCGTTTATTCCCGGATGCC	ACATCTTTACCCTTGGCCAC	20	20	50.0	50.0	58.1	57.8	0.7	3.9	0.323733	74457	75069	627-674	1.000000	81.4
psaJ-rpl33_p5	psaJ-rpl33	5	ATCGTTTATTCCCGGATGCC	CCCGGGGATTCCTTATTGAC	20	20	50.0	55.0	58.1	57.7	0.7	14.7	0.379734	74457	75069	693-740	1.000000	81.0
rpl20-rps12_p1	rpl20-rps12	1	AGTCTTGAATGAGCTCCCCT	TCTCCCGCTCTTAGAGGATG	20	20	50.0	55.0	58.0	58.0	12.1	11.5	0.047721	76405	77166	885-908	1.000000	82.5
rpl20-rps12_p2	rpl20-rps12	2	TGAGCTCCCCTAAAGGTTGA	TCTCCCGCTCTTAGAGGATG	20	20	50.0	55.0	58.0	58.0	12.1	11.5	0.050569	76405	77166	876-899	1.000000	82.5
rpl20-rps12_p3	rpl20-rps12	3	TTTTTGTTCGACGTCTCCGA	TCTCCCGCTCTTAGAGGATG	20	20	45.0	55.0	57.8	58.0	14.7	11.5	0.218522	76405	77166	842-865	1.000000	81.3
rpl20-rps12_p4	rpl20-rps12	4	TCTCCGAGCTGTATATCCCC	TCTCCCGCTCTTAGAGGATG	20	20	55.0	55.0	57.8	58.0	0.0	11.5	0.229217	76405	77166	829-852	1.000000	81.2
rpl20-rps12_p5	rpl20-rps12	5	ATCCCCGTCTAAGTCTGGTC	TCTCCCGCTCTTAGAGGATG	20	20	55.0	55.0	58.2	58.0	0.0	11.5	0.233766	76405	77166	815-838	1.000000	81.2
rpl16_p1	rpl16	1	GCGGTTGTTTTTCATCCTCG	CGATTGACCATTTCTTAGAATTGC	20	24	50.0	37.5	58.0	57.0	0.0	10.4	5.076010	89050	91365	2301-2370	1.000000	45.3
rpl16_p2	rpl16	2	GCGGTTGTTTTTCATCCTCG	CGATTGACCATTTCTTAGAATTGCT	20	25	50.0	36.0	58.0	58.2	0.0	10.4	5.246991	89050	91365	2301-2370	1.000000	45.3
rpl16_p3	rpl16	3	GCGGTTGTTTTTCATCCTCG	TCGATTGACCATTTCTTAGAATTGC	20	25	50.0	36.0	58.0	58.2	0.0	10.4	5.251062	89050	91365	2302-2371	1.000000	45.3
rpl16_p4	rpl16	4	GCGGTTGTTTTTCATCCTCG	TCTTTTTCCGTTAAATCAACCA	20	22	50.0	31.8	58.0	54.5	0.0	0.0	5.542435	89050	91365	2370-2439	1.000000	45.3
rpl16_p5	rpl16	5	GCGGTTGTTTTTCATCCTCG	CCGTTAAATCAACCAAAAATAAGCA	20	25	50.0	32.0	58.0	57.4	0.0	0.0	5.681239	89050	91365	2363-2432	1.000000	45.3
ndhF_p1	ndhF	1	TCAGTGAGGAGAAAACCGGA	AAGTTCAGAGTGTCATGCCC	20	20	50.0	50.0	58.3	57.8	9.5	0.0	0.479072	121170	123404	2858-2884	1.000000	76.6
ndhF_p2	ndhF	2	TCAGTGAGGAGAAAACCGGA	CAGAGTGTCATGCCCGTAAT	20	20	50.0	50.0	58.3	57.7	9.5	0.0	0.603869	121170	123404	2853-2879	1.000000	75.6
ndhF_p3	ndhF	3	CAGTGAGGAGAAAACCGGAA	AAGTTCAGAGTGTCATGCCC	20	20	50.0	50.0	57.5	57.8	9.5	0.0	0.734905	121170	123404	2857-2883	1.000000	74.6
ndhF_p4	ndhF	4	ATGCGTGCTCAAAGACGTAA	CAGAGTGTCATGCCCGTAAT	20	20	45.0	50.0	58.2	57.7	0.0	0.0	0.525170	121170	123404	2997-2997	0.500000	55.8
ndhF_p5	ndhF	5	TCTATGCGTGCTCAAAGACG	CAGAGTGTCATGCCCGTAAT	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.686913	121170	123404	3000-3000	0.500000	54.5
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCTGTCGGAATCGGAAT	GAAAAAGCCTTTACTGCGGC	20	20	50.0	50.0	58.6	58.3	0.0	0.0	0.932210	123405	124321	1076-1119	1.000000	75.7
ndhF-rpl32_p2	ndhF-rpl32	2	TTTTGTTGCTGTCGGAATCG	GAAAAAGCCTTTACTGCGGC	20	20	45.0	50.0	57.3	58.3	0.0	0.0	0.981190	123405	124321	1080-1123	1.000000	75.3
ndhF-rpl32_p3	ndhF-rpl32	3	TGCTGTCGGAATCGGAATAA	GAAAAAGCCTTTACTGCGGC	20	20	45.0	50.0	57.0	58.3	0.0	0.0	1.273495	123405	124321	1074-1117	1.000000	73.0
ndhF-rpl32_p4	ndhF-rpl32	4	TTGCTGTCGGAATCGGAATA	GAAAAAGCCTTTACTGCGGC	20	20	45.0	50.0	57.0	58.3	0.0	0.0	1.273495	123405	124321	1075-1118	1.000000	73.0
ndhF-rpl32_p5	ndhF-rpl32	5	TTTTTGTTGCTGTCGGAATCG	GAAAAAGCCTTTACTGCGGC	21	20	42.9	50.0	58.0	58.3	0.0	0.0	1.308290	123405	124321	1081-1124	1.000000	72.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTTTACCGAGCGTTCAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.0	58.0	0.0	0.0	1.050628	124496	125371	931-969	1.000000	75.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTTTACCGAGCGTTCAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.0	58.0	0.0	0.0	1.051998	124496	125371	932-970	1.000000	75.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTTTACCGAGCGTTCAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.0	57.9	0.0	0.0	1.167075	124496	125371	941-979	1.000000	74.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACCGAGCGTTCAAAAAGTTT	AGCGTGTCTACCGATTTCAC	20	20	40.0	50.0	56.8	58.0	0.0	0.0	1.225731	124496	125371	924-962	1.000000	73.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACCGAGCGTTCAAAAAGTTT	CAGCGTGTCTACCGATTTCA	20	20	40.0	50.0	56.8	58.0	0.0	0.0	1.227101	124496	125371	925-963	1.000000	73.9
ccsA-ndhD_p1	ccsA-ndhD	1	AGGGATAGGTCTACATAGTTATGGT	CTCGCTATCAGTTGACAGGG	25	20	40.0	55.0	57.9	57.8	4.3	0.0	5.333238	126519	127108	694-755	1.000000	44.3
ccsA-ndhD_p2	ccsA-ndhD	2	AGGGATAGGTCTACATAGTTATGGT	GTTGACAGGGCCCAAGTTAT	25	20	40.0	50.0	57.9	57.8	4.3	27.6	5.333273	126519	127108	684-745	1.000000	44.3
ccsA-ndhD_p3	ccsA-ndhD	3	AGGGATAGGTCTACATAGTTATGGT	CCGATTTCGTTCTCTCGCTA	25	20	40.0	50.0	57.9	57.5	4.3	0.0	5.587527	126519	127108	707-768	1.000000	44.3
ccsA-ndhD_p4	ccsA-ndhD	4	AGGGATAGGTCTACATAGTTATGGT	ATCCCGATTTCGTTCTCTCG	25	20	40.0	50.0	57.9	57.5	4.3	0.0	5.611044	126519	127108	710-771	1.000000	44.3
ccsA-ndhD_p5	ccsA-ndhD	5	GGGATAGGTCTACATAGTTATGGT	CTCGCTATCAGTTGACAGGG	24	20	41.7	55.0	56.6	57.8	4.3	0.0	5.650040	126519	127108	693-754	1.000000	44.3
psaC-ndhE_p1	psaC-ndhE	1	ATACATCTGTAGGGCAGGCT	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.736744	128995	130071	1189-1245	1.000000	78.6
psaC-ndhE_p2	psaC-ndhE	2	ATACATCTGTAGGGCAGGCT	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.736744	128995	130071	1188-1244	1.000000	78.6
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	128995	130071	1215-1271	1.000000	78.5
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	128995	130071	1214-1270	1.000000	78.5
psaC-ndhE_p5	psaC-ndhE	5	ATACATCTGTAGGGCAGGCT	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	57.9	58.8	0.0	6.0	0.875310	128995	130071	1187-1243	1.000000	77.5
rps15-ycf1_p1	rps15-ycf1	1	TCAACAGAACCCTTGTTTTCT	GCTTGTCTGAATCGCTATTGG	21	21	38.1	47.6	55.5	57.4	0.0	0.0	5.056821	135879	136515	801-844	1.000000	41.9
rps15-ycf1_p2	rps15-ycf1	2	TCAACAGAACCCTTGTTTTCT	CTTTGGCCCAATTACCGATT	21	20	38.1	45.0	55.5	56.4	0.0	0.0	5.096061	135879	136515	831-874	1.000000	41.9
rps15-ycf1_p3	rps15-ycf1	3	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGA	21	20	38.1	45.0	55.5	56.3	0.0	0.0	5.161573	135879	136515	828-871	1.000000	41.9
rps15-ycf1_p4	rps15-ycf1	4	TCAACAGAACCCTTGTTTTCTTC	GCTTGTCTGAATCGCTATTGG	23	21	39.1	47.6	57.4	57.4	0.0	0.0	5.175493	135879	136515	801-844	1.000000	41.9
rps15-ycf1_p5	rps15-ycf1	5	CAACAGAACCCTTGTTTTCTTCT	GCTTGTCTGAATCGCTATTGG	23	21	39.1	47.6	57.4	57.4	0.0	0.0	5.181734	135879	136515	800-843	1.000000	41.9