primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	rbcL	1	ATTCCAAGGTCCTGCAACAG	GCACCTTCGTTACGAGCTAA	20	20	50.0	50.0	58.1	58.0	0.0	7.7	0.098161	758	877	861-861	0.333333	52.5
rbcL_p2	rbcL	2	ATTCCAAGGTCCTGCAACAG	AACACCAGACATACGCATCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.109035	758	877	526-526	0.333333	52.5
rbcL_p3	rbcL	3	GCTGTTGCTGCTTTACGTTT	GCACCTTCGTTACGAGCTAA	20	20	45.0	50.0	57.9	58.0	0.0	7.7	0.132974	758	877	917-917	0.333333	52.3
rbcL_p4	rbcL	4	GCTGTTGCTGCTTTACGTTT	AACACCAGACATACGCATCC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.143847	758	877	582-582	0.333333	52.2
rbcL_p5	rbcL	5	ATTCCAAGGTCCTGCAACAG	TACCAACAACTGTACCAGCG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.242693	758	877	557-557	0.333333	51.4
bas1_p1	bas1	1	GTTAGTCGCGGATCAACAGT	GATGACCTTTGAAAGCGTGC	20	20	50.0	50.0	58.0	58.0	6.1	0.0	0.038727	5739	6350	1054-1054	0.333333	53.0
bas1_p2	bas1	2	ATCAAACGGGAACAGTTGCT	GATGACCTTTGAAAGCGTGC	20	20	45.0	50.0	58.0	58.0	4.6	0.0	0.049640	5739	6350	1002-1002	0.333333	52.9
bas1_p3	bas1	3	GTTAGTCGCGGATCAACAGT	CCGTTTTCGTGCTACGTTTC	20	20	50.0	50.0	58.0	58.1	6.1	0.0	0.079554	5739	6350	1199-1199	0.333333	52.7
bas1_p4	bas1	4	GTTAGTCGCGGATCAACAGT	CGTTTTCGTGCTACGTTTCC	20	20	50.0	50.0	58.0	58.1	6.1	0.0	0.079554	5739	6350	1198-1198	0.333333	52.7
bas1_p5	bas1	5	ATCAAACGGGAACAGTTGCT	CGTTTTCGTGCTACGTTTCC	20	20	45.0	50.0	58.0	58.1	4.6	0.0	0.090467	5739	6350	1146-1146	0.333333	52.6
rpl20-acpP_p1	rpl20-acpP	1	ACAAGCCTGTCCTGTAGAGT	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126329	6915	8584	2818-2818	0.333333	52.3
rpl20-acpP_p2	rpl20-acpP	2	GCACGCTTTCAAAGGTCATC	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.140321	6915	8584	2619-2619	0.333333	52.2
rpl20-acpP_p3	rpl20-acpP	3	CCTGTCCTGTAGAGTGGGAA	AGGATTTGAACCTACGGCAC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.170840	6915	8584	2813-2813	0.333333	52.0
rpl20-acpP_p4	rpl20-acpP	4	GAAACGTAGCACGAAAACGG	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	58.1	57.9	14.2	0.0	0.181148	6915	8584	2474-2474	0.333333	51.9
rpl20-acpP_p5	rpl20-acpP	5	GGAAACGTAGCACGAAAACG	AGGATTTGAACCTACGGCAC	20	20	50.0	50.0	58.1	57.9	14.2	0.0	0.181148	6915	8584	2475-2475	0.333333	51.9
rns-trnI(gau)_p1	rns-trnI(gau)	1	GTAACCGTACTGGAAGGTGC	ATTTGAACCAGAGACCTCGC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.382209	11715	11788	162-175	1.000000	88.9
rns-trnI(gau)_p2	rns-trnI(gau)	2	AGGTAACCGTACTGGAAGGT	ATTTGAACCAGAGACCTCGC	20	20	50.0	50.0	57.7	57.9	10.2	0.0	0.416325	11715	11788	164-177	1.000000	88.7
rns-trnI(gau)_p3	rns-trnI(gau)	3	GTAACCGTACTGGAAGGTGC	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	58.3	57.9	0.0	6.7	0.430050	11715	11788	176-189	1.000000	88.6
rns-trnI(gau)_p4	rns-trnI(gau)	4	AGGTAACCGTACTGGAAGGT	CTGGGCCATTCTGGATTTGA	20	20	50.0	50.0	57.7	57.9	10.2	6.7	0.464166	11715	11788	178-191	1.000000	88.3
rns-trnI(gau)_p5	rns-trnI(gau)	5	GGTAACCGTACTGGAAGGTG	ATTTGAACCAGAGACCTCGC	20	20	55.0	50.0	57.6	57.9	0.0	0.0	0.475778	11715	11788	163-176	1.000000	88.2
rrn5_p1	rrn5	1	ATCTCCCCCAAGAGTTCACA	GTAGCTGCACACGGTTACTT	20	20	50.0	50.0	58.0	58.2	0.0	1.0	0.252787	15085	15402	1357-1655	0.666667	76.6
rrn5_p2	rrn5	2	TCTCCCCCAAGAGTTCACAT	GTAGCTGCACACGGTTACTT	20	20	50.0	50.0	58.0	58.2	0.0	1.0	0.252787	15085	15402	1356-1654	0.666667	76.6
rrn5_p3	rrn5	3	TAGTAAACGGAACCACGCTG	GTAGCTGCACACGGTTACTT	20	20	50.0	50.0	57.9	58.2	0.0	1.0	0.276831	15085	15402	852-1150	0.666667	76.5
rrn5_p4	rrn5	4	GTAGTAAACGGAACCACGCT	GTAGCTGCACACGGTTACTT	20	20	50.0	50.0	57.9	58.2	0.0	1.0	0.278256	15085	15402	853-1151	0.666667	76.4
rrn5_p5	rrn5	5	TTCGGTCCATATCCGGTGTA	GTAGCTGCACACGGTTACTT	20	20	50.0	50.0	57.9	58.2	0.0	1.0	0.278299	15085	15402	1212-1510	0.666667	76.4
syfB_p1	syfB	1	TTTAATAAGGGCGAGCGACC	CTTTGACTCCGCCATTCGTA	20	20	50.0	50.0	58.1	58.0	6.7	0.0	0.060551	26914	29031	2996-2996	0.333333	52.8
syfB_p2	syfB	2	TTTAATAAGGGCGAGCGACC	TTTGACTCCGCCATTCGTAG	20	20	50.0	50.0	58.1	58.0	6.7	0.0	0.060551	26914	29031	2995-2995	0.333333	52.8
syfB_p3	syfB	3	TTTAATAAGGGCGAGCGACC	AAGACGGTAGCACAGGTACT	20	20	50.0	50.0	58.1	58.1	6.7	6.4	0.135358	26914	29031	2831-2831	0.333333	52.3
syfB_p4	syfB	4	TTTAATAAGGGCGAGCGACC	CAAAAGACGGTAGCACAGGT	20	20	50.0	50.0	58.1	58.1	6.7	0.0	0.177015	26914	29031	2834-2834	0.333333	51.9
syfB_p5	syfB	5	TTTAATAAGGGCGAGCGACC	GGTCAAAAGACGGTAGCACA	20	20	50.0	50.0	58.1	58.1	6.7	0.0	0.182271	26914	29031	2837-2837	0.333333	51.9
rpl2_p1	rpl2	1	AAAGGTGGGGGCGTTATTTT	CAATGTTAATGGGCCTTGCG	20	20	45.0	50.0	58.0	58.1	0.0	4.5	0.093126	35116	35943	1925-1925	0.333333	52.6
rpl2_p2	rpl2	2	AAGGTGGGGGCGTTATTTTT	CAATGTTAATGGGCCTTGCG	20	20	45.0	50.0	58.0	58.1	0.0	4.5	0.093126	35116	35943	1924-1924	0.333333	52.6
rpl2_p3	rpl2	3	AAAGGTGGGGGCGTTATTTT	ATGTTAATGGGCCTTGCGAA	20	20	45.0	45.0	58.0	58.2	0.0	4.5	0.172349	35116	35943	1923-1923	0.333333	52.0
rpl2_p4	rpl2	4	AAGGTGGGGGCGTTATTTTT	AATGTTAATGGGCCTTGCGA	20	20	45.0	45.0	58.0	58.2	0.0	4.5	0.172349	35116	35943	1923-1923	0.333333	52.0
rpl2_p5	rpl2	5	AAGGTGGGGGCGTTATTTTT	ATGTTAATGGGCCTTGCGAA	20	20	45.0	45.0	58.0	58.2	0.0	4.5	0.172349	35116	35943	1922-1922	0.333333	52.0
trnL(uaa)-trnC(gca)_p1	trnL(uaa)-trnC(gca)	1	CTCACGCACTATCACTAGGC	CAGTTCGAATCTGGGTGCC	20	19	55.0	57.9	57.9	58.5	0.0	15.7	1.655659	49963	50030	151-156	1.000000	78.4
trnL(uaa)-trnC(gca)_p2	trnL(uaa)-trnC(gca)	2	CTCACGCACTATCACTAGGC	CCAGTTCGAATCTGGGTGC	20	19	55.0	57.9	57.9	58.5	0.0	26.3	1.655659	49963	50030	152-157	1.000000	78.4
trnL(uaa)-trnC(gca)_p3	trnL(uaa)-trnC(gca)	3	TAAGTCCGTCGTGTCTACCA	CAGTTCGAATCTGGGTGCC	20	19	50.0	57.9	57.8	58.5	0.0	15.7	1.712223	49963	50030	122-127	1.000000	78.3
trnL(uaa)-trnC(gca)_p4	trnL(uaa)-trnC(gca)	4	ACCCTCACGCACTATCACTA	CAGTTCGAATCTGGGTGCC	20	19	50.0	57.9	57.9	58.5	0.0	15.7	1.668069	49963	50030	154-159	1.000000	78.2
trnL(uaa)-trnC(gca)_p5	trnL(uaa)-trnC(gca)	5	ACCCTCACGCACTATCACTA	CCAGTTCGAATCTGGGTGC	20	19	50.0	57.9	57.9	58.5	0.0	26.3	1.668069	49963	50030	155-160	1.000000	78.2
trnI(gau)-rns_p1	trnI(gau)-rns	1	ATTTGAACCAGAGACCTCGC	GTAACCGTACTGGAAGGTGC	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.382209	62177	62250	162-175	1.000000	88.9
trnI(gau)-rns_p2	trnI(gau)-rns	2	ATTTGAACCAGAGACCTCGC	AGGTAACCGTACTGGAAGGT	20	20	50.0	50.0	57.9	57.7	0.0	10.2	0.416325	62177	62250	164-177	1.000000	88.7
trnI(gau)-rns_p3	trnI(gau)-rns	3	CTGGGCCATTCTGGATTTGA	GTAACCGTACTGGAAGGTGC	20	20	50.0	55.0	57.9	58.3	6.7	0.0	0.430050	62177	62250	176-189	1.000000	88.6
trnI(gau)-rns_p4	trnI(gau)-rns	4	CTGGGCCATTCTGGATTTGA	AGGTAACCGTACTGGAAGGT	20	20	50.0	50.0	57.9	57.7	6.7	10.2	0.464166	62177	62250	178-191	1.000000	88.3
trnI(gau)-rns_p5	trnI(gau)-rns	5	ATTTGAACCAGAGACCTCGC	GGTAACCGTACTGGAAGGTG	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.475778	62177	62250	163-176	1.000000	88.2
acpP-rpl20_p1	acpP-rpl20	1	AGGATTTGAACCTACGGCAC	ACAAGCCTGTCCTGTAGAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.126329	65381	67050	2818-2818	0.333333	52.3
acpP-rpl20_p2	acpP-rpl20	2	AGGATTTGAACCTACGGCAC	GCACGCTTTCAAAGGTCATC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.140321	65381	67050	2619-2619	0.333333	52.2
acpP-rpl20_p3	acpP-rpl20	3	AGGATTTGAACCTACGGCAC	CCTGTCCTGTAGAGTGGGAA	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.170840	65381	67050	2813-2813	0.333333	52.0
acpP-rpl20_p4	acpP-rpl20	4	AGGATTTGAACCTACGGCAC	GAAACGTAGCACGAAAACGG	20	20	50.0	50.0	57.9	58.1	0.0	14.2	0.181148	65381	67050	2474-2474	0.333333	51.9
acpP-rpl20_p5	acpP-rpl20	5	AGGATTTGAACCTACGGCAC	GGAAACGTAGCACGAAAACG	20	20	50.0	50.0	57.9	58.1	0.0	14.2	0.181148	65381	67050	2475-2475	0.333333	51.9
psbC_p1	psbC	1	CCGATTTGTTCTGGGTTCGA	CAAGTTTTGGTGTTGCTGCA	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.204026	81438	82853	2074-2075	0.666667	73.4
psbC_p2	psbC	2	CTTCCCATTCTCCAGGTGTG	CAAGTTTTGGTGTTGCTGCA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.127162	81438	82853	2540-2540	0.333333	52.3
psbC_p3	psbC	3	CTTCCCATTCTCCAGGTGTG	GATGGTGATGCTGCAAACAC	20	20	55.0	50.0	57.9	58.0	0.0	24.6	0.133103	81438	82853	2379-2379	0.333333	52.3
psbC_p4	psbC	4	CTTCCCATTCTCCAGGTGTG	TCCGTGCTTTTACACCAACA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.159619	81438	82853	2357-2357	0.333333	52.1
psbC_p5	psbC	5	CTTCCCATTCTCCAGGTGTG	CTTTTATGGGGTCCTGAGGC	20	20	55.0	55.0	57.9	57.9	0.0	0.0	0.178394	81438	82853	2781-2781	0.333333	51.9
petA_p1	petA	1	TCAACATGCGCAGGTAAAGT	TTTAACCCCGTCAACAGACC	20	20	45.0	50.0	58.1	57.7	29.5	0.0	0.368390	93869	94813	1305-1305	0.333333	50.4
petA_p2	petA	2	CAGGTGCTTGTTCAACATGC	TTTAACCCCGTCAACAGACC	20	20	50.0	50.0	58.2	57.7	19.1	0.0	0.484628	93869	94813	1316-1316	0.333333	49.5
petA_p3	petA	3	GCAGGTGCTTGTTCAACATG	TTTAACCCCGTCAACAGACC	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.484628	93869	94813	1317-1317	0.333333	49.5
petA_p4	petA	4	TCAACATGCGCAGGTAAAGT	AACTTTCGCCCGGTGAATAT	20	20	45.0	45.0	58.1	57.6	29.5	14.2	0.522433	93869	94813	1741-1741	0.333333	49.2
petA_p5	petA	5	TCAACATGCGCAGGTAAAGT	ACTTTCGCCCGGTGAATATT	20	20	45.0	45.0	58.1	57.6	29.5	14.2	0.522433	93869	94813	1740-1740	0.333333	49.2
rpoB_p1	rpoB	1	GACGACGGCAGCTACTTTTA	CCAACCGATCTTACTCGTCG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.189474	100405	100524	1581-1581	0.333333	51.8
rpoB_p2	rpoB	2	TTAATGACGACGGCAGCTAC	CCAACCGATCTTACTCGTCG	20	20	50.0	55.0	58.1	58.2	10.3	0.0	0.258899	100405	100524	1586-1586	0.333333	51.3
rpoB_p3	rpoB	3	CTTAATGACGACGGCAGCTA	CCAACCGATCTTACTCGTCG	20	20	50.0	55.0	57.8	58.2	10.3	0.0	0.379972	100405	100524	1587-1587	0.333333	50.3
rpoB_p4	rpoB	4	TTACCCTTAATGACGACGGC	CCAACCGATCTTACTCGTCG	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.474583	100405	100524	1592-1592	0.333333	49.5
rpoB_p5	rpoB	5	GACGACGGCAGCTACTTTTA	TCAGGAATAATGGTTGCCGT	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.812428	100405	100524	1074-1074	0.333333	46.8
rpoC2_p1	rpoC2	1	GGCTTGAAGAAGAAACTCACC	GCATCAGACGAGGTATTGAGA	21	21	47.6	47.6	57.1	57.3	0.0	0.0	3.623414	108056	108175	1143-1143	0.333333	24.3
rpoC2_p2	rpoC2	2	GGCTTGAAGAAGAAACTCACC	AGCATCAGACGAGGTATTGAG	21	21	47.6	47.6	57.1	57.3	0.0	0.0	3.630522	108056	108175	1144-1144	0.333333	24.3
rpoC2_p3	rpoC2	3	GGCTTGAAGAAGAAACTCACC	ACGAGAAGGAATCCATCGAT	21	20	47.6	45.0	57.1	56.1	0.0	22.3	3.778778	108056	108175	1007-1007	0.333333	23.1
rpoC2_p4	rpoC2	4	GGCTTGAAGAAGAAACTCACC	AGCATCAGACGAGGTATTGA	21	20	47.6	45.0	57.1	56.1	0.0	0.0	3.847549	108056	108175	1144-1144	0.333333	22.6
rpoC2_p5	rpoC2	5	GGCTTGAAGAAGAAACTCACC	AGGTTGACCCTTTTGTAGTGT	21	21	47.6	42.9	57.1	57.1	0.0	0.0	3.851654	108056	108175	932-932	0.333333	22.5