primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-rps16_p1	trnV-UAC-rps16	1	TAGGCTACAAATGGACCCCA	CCACCGAGATTGTTCACGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036117	10999	12120	640-1683	0.306122	64.0
trnV-UAC-rps16_p2	trnV-UAC-rps16	2	AGCTTGACTTTTGGTTCGGT	CCACCGAGATTGTTCACGAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.062927	10999	12120	678-1722	0.306122	63.7
trnV-UAC-rps16_p3	trnV-UAC-rps16	3	GGTTCGAATCCGTATAGCCC	CCACCGAGATTGTTCACGAT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.086124	10999	12120	188-1231	0.306122	63.6
trnV-UAC-rps16_p4	trnV-UAC-rps16	4	TAGGCTACAAATGGACCCCA	GGGCTGTGTGAGAAACTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058653	10999	12120	1506-1651	0.265306	55.8
trnV-UAC-rps16_p5	trnV-UAC-rps16	5	AGCTTGACTTTTGGTTCGGT	GGGCTGTGTGAGAAACTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.085464	10999	12120	1545-1690	0.265306	55.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	14732	15740	550-1151	1.000000	62.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.2	57.3	0.0	0.0	3.819906	14732	15740	553-1154	1.000000	61.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.2	57.3	0.0	0.0	3.819906	14732	15740	552-1153	1.000000	61.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.2	56.2	0.0	0.0	3.927661	14732	15740	551-1152	1.000000	60.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	20	20	50.0	45.0	58.2	54.0	0.0	0.0	4.162424	14732	15740	536-1137	1.000000	58.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGAGCCCATATCCTTGCTTT	TTGTTGTTGATCAGGCGACA	20	20	50.0	45.0	58.0	58.0	0.0	14.7	0.066711	34472	35712	1797-1878	0.877551	86.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCCTTAGGGTCCAACTCTGA	AGAATCCTTCCTTGGAGCCT	20	20	55.0	50.0	58.0	58.0	9.6	0.0	0.069017	34472	35712	1915-1976	0.448980	69.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTTAGGGTCCAACTCTG	AGAATCCTTCCTTGGAGCCT	20	20	55.0	50.0	58.0	58.0	18.0	0.0	0.069017	34472	35712	1916-1977	0.448980	69.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCGGGGCTTTGCAATATTTG	AGAATCCTTCCTTGGAGCCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.083013	34472	35712	1882-1943	0.448980	69.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CGAGCCCATATCCTTGCTTT	AGAATCCTTCCTTGGAGCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048767	34472	35712	2002-2063	0.428571	68.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	35784	36654	792-975	1.000000	89.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	AGTTCAAATCTGGGTGTCGC	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.5	58.0	0.0	0.0	0.482286	35784	36654	767-950	1.000000	88.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.4	57.8	14.7	0.0	0.546619	35784	36654	786-969	0.979592	86.8
trnC-GCA-petN_p4	trnC-GCA-petN	4	AGTTCAAATCTGGGTGTCGC	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.667370	35784	36654	761-944	0.979592	85.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTCAAATCTGGGTGTCGCC	TTAAAGCAGCCCAAGCAAGA	19	20	52.6	45.0	58.1	58.0	0.0	0.0	1.064834	35784	36654	765-948	1.000000	83.5
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.366926	39772	40336	382-641	1.000000	89.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.385076	39772	40336	361-620	1.000000	88.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CCTGAACCACTAGACGATGG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.689795	39772	40336	358-617	1.000000	86.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CTGAACCACTAGACGATGGG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.689795	39772	40336	357-616	1.000000	86.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGAACCACTAGACGATGGGG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.8	57.9	0.0	0.0	0.912002	39772	40336	356-615	1.000000	84.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTTCAAATCCGATAAGGGGCT	TCTACCAAGGGCTATTGTCA	21	20	47.6	45.0	58.1	55.5	0.0	0.0	3.530679	40409	41309	927-964	0.979592	62.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TTCAAATCCGATAAGGGGCT	TCTACCAAGGGCTATTGTCA	20	20	45.0	45.0	56.6	55.5	0.0	0.0	3.881618	40409	41309	926-963	0.979592	60.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTTCAAATCCGATAAGGGGC	TCTACCAAGGGCTATTGTCA	20	20	50.0	45.0	56.6	55.5	0.0	0.0	3.907014	40409	41309	927-964	0.979592	59.9
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTTCAAATCCGATAAGGGGCT	ATCTACCAAGGGCTATTGTCA	21	21	47.6	42.9	58.1	56.1	0.0	0.0	3.947770	40409	41309	928-965	0.979592	59.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTTCAAATCCGATAAGGGGCT	TCTACCAAGGGCTATTGTCAT	21	21	47.6	42.9	58.1	56.1	0.0	0.0	3.947770	40409	41309	927-964	0.979592	59.6
psaA-pafI_p1	psaA-pafI	1	ATGCCACTCAGCCAAAGAAA	GTTGAAGATCACGAGACGCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.016379	50304	50932	819-936	0.979592	91.1
psaA-pafI_p2	psaA-pafI	2	TGCCACTCAGCCAAAGAAAT	GTTGAAGATCACGAGACGCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.016379	50304	50932	818-935	0.979592	91.1
psaA-pafI_p3	psaA-pafI	3	GCTGGGTCCAATGTGAGTAG	GTTGAAGATCACGAGACGCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.045866	50304	50932	889-1006	0.959184	90.0
psaA-pafI_p4	psaA-pafI	4	ATGCCACTCAGCCAAAGAAA	AACCCACCTATTCTGACCGA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.053367	50304	50932	961-1078	0.918367	88.3
psaA-pafI_p5	psaA-pafI	5	TGCCACTCAGCCAAAGAAAT	AACCCACCTATTCTGACCGA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.053367	50304	50932	960-1077	0.918367	88.3
pafI-trnS-GGA_p1	pafI-trnS-GGA	1	CCATTTATCCGTGATCTAGGCA	GACCGCTCGGCCATCTCTC	22	19	45.5	68.4	57.7	62.5	0.0	8.4	7.764006	52970	54355	318-1947	0.918367	48.7
pafI-trnS-GGA_p2	pafI-trnS-GGA	2	CCATTTATCCGTGATCTAGGCA	ACCGCTCGGCCATCTCTC	22	18	45.5	66.7	57.7	61.5	0.0	8.4	7.803851	52970	54355	317-1946	0.918367	48.7
pafI-trnS-GGA_p3	pafI-trnS-GGA	3	CCATTTATCCGTGATCTAGGCA	GACCGCTCGGCCATCTCT	22	18	45.5	66.7	57.7	61.5	0.0	8.4	7.803851	52970	54355	318-1947	0.918367	48.7
pafI-trnS-GGA_p4	pafI-trnS-GGA	4	TCCATTTATCCGTGATCTAGGC	GACCGCTCGGCCATCTCTC	22	19	45.5	68.4	57.5	62.5	0.0	8.4	8.029445	52970	54355	319-1948	0.918367	48.7
pafI-trnS-GGA_p5	pafI-trnS-GGA	5	TCCATTTATCCGTGATCTAGGC	ACCGCTCGGCCATCTCTC	22	18	45.5	66.7	57.5	61.5	0.0	8.4	8.069290	52970	54355	318-1947	0.918367	48.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	55770	56436	681-771	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	55770	56436	689-779	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	55770	56436	713-803	1.000000	90.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	55770	56436	721-811	1.000000	89.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	55770	56436	695-785	1.000000	89.0
ndhC-psaI_p1	ndhC-psaI	1	GAAACTCATCGCCCATGGAT	CGGAACTACTAGGCCCACTA	20	20	50.0	55.0	58.0	57.9	15.2	0.0	0.090098	59757	62022	919-2963	0.693878	79.0
ndhC-psaI_p2	ndhC-psaI	2	GAAACTCATCGCCCATGGAT	CGGTTTCATACATCTGCGGA	20	20	50.0	50.0	58.0	58.1	15.2	0.0	0.092539	59757	62022	1390-2957	0.326531	64.3
ndhC-psaI_p3	ndhC-psaI	3	GAAACTCATCGCCCATGGAT	TCGGTTTCATACATCTGCGG	20	20	50.0	50.0	58.0	58.1	15.2	0.0	0.092539	59757	62022	1391-2958	0.326531	64.3
ndhC-psaI_p4	ndhC-psaI	4	CTGTGCCGGCTGTTAAAATG	CGGAACTACTAGGCCCACTA	20	20	50.0	55.0	58.0	57.9	29.7	0.0	0.081717	59757	62022	1324-2991	0.306122	63.6
ndhC-psaI_p5	ndhC-psaI	5	GCTAGAAAGCTTCTCCGTCC	GTTTTTCGAGACCCCGCTAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.090717	59757	62022	1529-2995	0.102041	55.4
psbE-petL_p1	psbE-petL	1	CATTGCGGATATTGACCCCA	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056973	67746	68975	2019-2199	0.979592	90.7
psbE-petL_p2	psbE-petL	2	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	20	20	45.0	50.0	58.0	58.0	0.0	2.6	0.059731	67746	68975	2108-2288	0.836735	85.0
psbE-petL_p3	psbE-petL	3	CATTGCGGATATTGACCCCA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	0.0	2.6	0.036173	67746	68975	2041-2221	0.816327	84.4
psbE-petL_p4	psbE-petL	4	ATGGAATTCCTTGTCGGCTC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	24.7	2.6	0.060150	67746	68975	1871-2051	0.816327	84.2
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.9	58.0	6.4	2.6	0.067362	67746	68975	1782-1958	0.714286	80.0
clpP1_p1	clpP1	1	TCAACAATTGGTGGCATGGA	GTATCAATCCGCAATGCAGC	20	20	45.0	50.0	58.0	58.0	23.0	5.9	0.048909	73601	75835	2786-2833	0.285714	63.0
clpP1_p2	clpP1	2	AACAATTGGTGGCATGGACT	GTATCAATCCGCAATGCAGC	20	20	45.0	50.0	58.0	58.0	10.8	5.9	0.056114	73601	75835	2784-2831	0.285714	63.0
clpP1_p3	clpP1	3	TCAACAATTGGTGGCATGGA	TTTTCTACGGGCCAATGCTT	20	20	45.0	45.0	58.0	58.1	23.0	4.4	0.088933	73601	75835	2751-2977	0.285714	62.7
clpP1_p4	clpP1	4	TACAAGGGGGAAGGTAAGGG	GTATCAATCCGCAATGCAGC	20	20	55.0	50.0	58.0	58.0	0.0	5.9	0.066633	73601	75835	2581-2619	0.244898	57.0
clpP1_p5	clpP1	5	ATTTGCCCCTGGTTCCATTT	GTATCAATCCGCAATGCAGC	20	20	45.0	50.0	58.0	58.0	0.0	5.9	0.085411	73601	75835	2819-2857	0.204082	52.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGATCCATGCATATTGATATGT	GTACGTTTCTTTGGAACCGC	22	20	31.8	50.0	53.1	57.7	0.0	0.0	7.165997	117770	120308	1987-2739	0.346939	25.9
ndhF-rpl32_p2	ndhF-rpl32	2	ATGATCCATGCATATTGATATGT	GTACGTTTCTTTGGAACCGC	23	20	30.4	50.0	53.8	57.7	0.0	0.0	7.537749	117770	120308	1988-2740	0.346939	25.9
ndhF-rpl32_p3	ndhF-rpl32	3	TGCATATTGATATGTATGTTCCA	GTACGTTTCTTTGGAACCGC	23	20	30.4	50.0	53.7	57.7	11.5	0.0	7.629713	117770	120308	1980-2732	0.346939	25.9
ndhF-rpl32_p4	ndhF-rpl32	4	TGATCCATGCATATTGATATGT	TACGTTTCTTTGGAACCGCC	22	20	31.8	50.0	53.1	58.8	0.0	0.0	7.632212	117770	120308	1986-2738	0.346939	25.9
ndhF-rpl32_p5	ndhF-rpl32	5	ATGATCCATGCATATTGATATGT	TACGTTTCTTTGGAACCGCC	23	20	30.4	50.0	53.8	58.8	0.0	0.0	8.003964	117770	120308	1987-2739	0.346939	25.9
ndhD_p1	ndhD	1	CGCAAATGAAAGAGGCAGTG	CCGCTATCATGAGCAGATCC	20	20	50.0	55.0	58.0	58.0	0.0	10.5	0.034222	122626	124134	1814-2656	1.000000	91.7
ndhD_p2	ndhD	2	CGCAATTGTGGCTTCTCTTG	CCGCTATCATGAGCAGATCC	20	20	50.0	55.0	58.0	58.0	11.3	10.5	0.034222	122626	124134	1529-2371	1.000000	91.7
ndhD_p3	ndhD	3	CGCAAATGAAAGAGGCAGTG	AATTGCTTCTGCCCCAAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.040871	122626	124134	1557-2399	0.979592	90.9
ndhD_p4	ndhD	4	CGCAATTGTGGCTTCTCTTG	AAATTGCTTCTGCCCCAAGA	20	20	50.0	45.0	58.0	58.0	11.3	0.0	0.040871	122626	124134	1273-2115	0.979592	90.9
ndhD_p5	ndhD	5	CGCAATTGTGGCTTCTCTTG	AATTGCTTCTGCCCCAAGAA	20	20	50.0	45.0	58.0	58.0	11.3	0.0	0.040871	122626	124134	1272-2114	0.979592	90.9
ycf1-rps15_p1	ycf1-rps15	1	TCCACGCTTGAGAATTGGTT	CGTTTTCCCCGATTCAGGAT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.308739	147378	149779	795-2813	0.285714	55.0
ycf1-rps15_p2	ycf1-rps15	2	CCACGCTTGAGAATTGGTTG	CGTTTTCCCCGATTCAGGAT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.374941	147378	149779	794-2812	0.285714	54.4
ycf1-rps15_p3	ycf1-rps15	3	TCCACGCTTGAGAATTGGTT	TTCCCCGATTCAGGATCAGT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.390777	147378	149779	791-2809	0.285714	54.3
ycf1-rps15_p4	ycf1-rps15	4	CCACGCTTGAGAATTGGTTG	TTCCCCGATTCAGGATCAGT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.456980	147378	149779	790-2808	0.285714	53.8
ycf1-rps15_p5	ycf1-rps15	5	TCCACGCTTGAGAATTGGTT	ATGCATTGATGGGTGGTGAG	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.498787	147378	149779	867-2885	0.285714	53.3
ndhD_p1	ndhD	1	CCGCTATCATGAGCAGATCC	CGCAAATGAAAGAGGCAGTG	20	20	55.0	50.0	58.0	58.0	10.5	0.0	0.034222	156155	157663	1814-2656	0.979592	90.9
ndhD_p2	ndhD	2	CCGCTATCATGAGCAGATCC	CGCAATTGTGGCTTCTCTTG	20	20	55.0	50.0	58.0	58.0	10.5	11.3	0.034222	156155	157663	1529-2371	0.979592	90.9
ndhD_p3	ndhD	3	AAATTGCTTCTGCCCCAAGA	CGCAATTGTGGCTTCTCTTG	20	20	45.0	50.0	58.0	58.0	0.0	11.3	0.040871	156155	157663	1273-2115	0.959184	90.0
ndhD_p4	ndhD	4	AATTGCTTCTGCCCCAAGAA	CGCAAATGAAAGAGGCAGTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.040871	156155	157663	1557-2399	0.959184	90.0
ndhD_p5	ndhD	5	AATTGCTTCTGCCCCAAGAA	CGCAATTGTGGCTTCTCTTG	20	20	45.0	50.0	58.0	58.0	0.0	11.3	0.040871	156155	157663	1272-2114	0.959184	90.0
rpl32-ndhF_p1	rpl32-ndhF	1	GTACGTTTCTTTGGAACCGC	TGATCCATGCATATTGATATGT	20	22	50.0	31.8	57.7	53.1	0.0	0.0	7.165997	159981	162519	1987-2739	0.346939	25.9
rpl32-ndhF_p2	rpl32-ndhF	2	GTACGTTTCTTTGGAACCGC	ATGATCCATGCATATTGATATGT	20	23	50.0	30.4	57.7	53.8	0.0	0.0	7.537749	159981	162519	1988-2740	0.346939	25.9
rpl32-ndhF_p3	rpl32-ndhF	3	GTACGTTTCTTTGGAACCGC	TGCATATTGATATGTATGTTCCA	20	23	50.0	30.4	57.7	53.7	0.0	11.5	7.629713	159981	162519	1980-2732	0.346939	25.9
rpl32-ndhF_p4	rpl32-ndhF	4	TACGTTTCTTTGGAACCGCC	TGATCCATGCATATTGATATGT	20	22	50.0	31.8	58.8	53.1	0.0	0.0	7.632212	159981	162519	1986-2738	0.346939	25.9
rpl32-ndhF_p5	rpl32-ndhF	5	TACGTTTCTTTGGAACCGCC	ATGATCCATGCATATTGATATGT	20	23	50.0	30.4	58.8	53.8	0.0	0.0	8.003964	159981	162519	1987-2739	0.346939	25.9