primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	75	354	392-399	1.000000	79.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	75	354	406-413	1.000000	79.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	75	354	405-412	1.000000	79.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	75	354	407-414	1.000000	79.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	75	354	393-400	1.000000	79.0
atpH-atpI_p1	atpH-atpI	1	CTCTACAGCTTGACCTGCAG	CGCGGCGTATATAGGTGAAT	20	20	55.0	50.0	58.0	57.7	6.8	15.4	0.280730	13672	14828	1299-1308	1.000000	79.4
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	CGCGGCGTATATAGGTGAAT	20	20	45.0	50.0	58.0	57.7	1.4	15.4	0.311756	13672	14828	1220-1229	1.000000	79.3
atpH-atpI_p3	atpH-atpI	3	GCGATACCCTCTACAGCTTG	CGCGGCGTATATAGGTGAAT	20	20	55.0	50.0	57.9	57.7	0.0	15.4	0.414905	13672	14828	1307-1316	1.000000	78.3
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	CGCGGCGTATATAGGTGAAT	20	20	45.0	50.0	58.2	57.7	1.4	15.4	0.457295	13672	14828	1221-1230	1.000000	78.1
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	CGCGGCGTATATAGGTGAAT	20	20	50.0	50.0	57.8	57.7	0.0	15.4	0.487756	13672	14828	1235-1244	1.000000	77.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCGCCTACACAAGCAAATTG	CTGGGGGTTTCCGAAAAGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029040	27028	28308	2521-2550	1.000000	81.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCGCCTACACAAGCAAATTG	TTTCCTATCAGGCTCCCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038937	27028	28308	2342-2370	1.000000	81.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCGCCTACACAAGCAAATTG	GGGGAATATTTCGGCGAGTT	20	20	50.0	50.0	58.0	58.0	0.0	10.5	0.055574	27028	28308	2087-2115	1.000000	81.5
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCGGGGCTTTGCAATATTTG	CTGGGGGTTTCCGAAAAGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071668	27028	28308	2209-2238	1.000000	81.5
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGGGGCTTTGCAATATTTG	TTTCCTATCAGGCTCCCCAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081565	27028	28308	2030-2058	1.000000	81.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGTAGAAAAGCGGTAGGAACT	AAATTTTCTGCCAGACCCCC	21	20	42.9	50.0	56.4	58.4	0.0	0.0	2.966614	30562	31262	833-872	1.000000	60.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGCGGTAGGAACTAGAATGA	AAATTTTCTGCCAGACCCCC	20	20	45.0	50.0	55.4	58.4	0.0	0.0	2.985213	30562	31262	825-864	1.000000	60.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	56.4	58.5	0.0	5.4	3.088455	30562	31262	794-833	1.000000	59.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.4	58.5	0.0	5.4	3.107053	30562	31262	786-825	1.000000	59.6
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGCGGTAGGAACTAGAATGAA	AAATTTTCTGCCAGACCCCC	21	20	42.9	50.0	56.2	58.4	0.0	0.0	3.177156	30562	31262	825-864	1.000000	58.8
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	37439	37585	249-250	1.000000	80.6
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	37439	37585	250-251	1.000000	80.6
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGAAGATGCGGGTTCGATTC	TTCAGTTCTTCTCTTCGGCG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.124668	37439	37585	271-272	1.000000	80.2
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	GAGAAGATGCGGGTTCGATT	TTCAGTTCTTCTCTTCGGCG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.124668	37439	37585	272-273	1.000000	80.2
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124730	37439	37585	290-291	1.000000	80.1
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	42722	43363	817-820	1.000000	79.6
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	42722	43363	799-802	1.000000	79.6
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	42722	43363	819-822	1.000000	78.8
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	42722	43363	813-816	1.000000	77.9
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.377504	42722	43363	815-818	1.000000	77.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	TGCATGTTGGGTCTGTGAAA	20	20	50.0	45.0	57.9	57.9	5.2	0.0	0.203757	51331	52467	1333-1335	1.000000	79.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AAAGTGCATGTTGGGTCTGT	20	20	50.0	45.0	57.9	57.9	5.2	0.0	0.211101	51331	52467	1337-1339	1.000000	79.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	51331	52467	1268-1270	1.000000	78.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	GCATGTTGGGTCTGTGAAAC	20	20	50.0	50.0	57.9	57.6	5.2	0.0	0.561599	51331	52467	1332-1334	1.000000	76.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	5.2	0.0	0.680379	51331	52467	1267-1269	1.000000	75.4
trnM-CAU-psaI_p1	trnM-CAU-psaI	1	CCCGCCGTATGAAAGCAATA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.4	58.0	0.0	26.9	0.438329	57800	60247	2561-2652	1.000000	77.7
trnM-CAU-psaI_p2	trnM-CAU-psaI	2	CCCGCCGTATGAAAGCAATA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.4	57.9	0.0	26.8	0.514863	57800	60247	2567-2658	1.000000	77.1
trnM-CAU-psaI_p3	trnM-CAU-psaI	3	CCGCCGTATGAAAGCAATAC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.3	58.0	0.0	26.9	0.690282	57800	60247	2560-2651	1.000000	75.7
trnM-CAU-psaI_p4	trnM-CAU-psaI	4	CCGCCGTATGAAAGCAATAC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.3	57.9	0.0	26.8	0.766816	57800	60247	2566-2657	1.000000	75.1
trnM-CAU-psaI_p5	trnM-CAU-psaI	5	TTTGAACCAATGACTCCCGC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.8	58.0	0.0	26.9	0.783101	57800	60247	2576-2667	1.000000	74.9
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	57.8	56.2	0.0	0.0	1.948359	64120	64952	851-1111	1.000000	69.4
petA-psbJ_p2	petA-psbJ	2	CCATTACGTGTTCAAGGCCT	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	0.0	0.0	1.972289	64120	64952	866-1126	1.000000	69.2
petA-psbJ_p3	petA-psbJ	3	TCCATTACGTGTTCAAGGCC	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	0.0	0.0	1.977431	64120	64952	867-1127	1.000000	69.2
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	GCCGATACTACTGGAAGGATT	20	21	50.0	47.6	57.8	57.0	0.0	0.0	2.186450	64120	64952	851-1111	1.000000	67.5
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTTCAAGGCCT	GCCGATACTACTGGAAGGATT	20	21	50.0	47.6	58.2	57.0	0.0	0.0	2.210380	64120	64952	866-1126	1.000000	67.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.194794	65730	66950	1364-1368	1.000000	71.6
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.194794	65730	66950	1363-1367	1.000000	71.6
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	20	20	50.0	45.0	57.8	56.9	24.9	0.0	1.299803	65730	66950	1287-1291	1.000000	70.8
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	20	20	50.0	45.0	57.8	56.9	24.9	0.0	1.299803	65730	66950	1286-1290	1.000000	70.8
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AGCCGCTAGTAGAAAACCAA	20	20	45.0	45.0	58.2	56.9	0.0	0.0	1.314480	65730	66950	1372-1376	1.000000	70.6
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	67524	67692	249-249	1.000000	81.2
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	67524	67692	238-238	1.000000	81.2
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	67524	67692	236-236	1.000000	81.2
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	67524	67692	239-239	1.000000	81.2
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	67524	67692	250-250	1.000000	81.1
rpl20-rps12_p1	rpl20-rps12	1	GAGCCCCTCGAAAGTTTGAT	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.236459	70063	70860	906-909	1.000000	78.9
rpl20-rps12_p2	rpl20-rps12	2	AGCCCCTCGAAAGTTTGATG	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.294731	70063	70860	905-908	1.000000	78.4
rpl20-rps12_p3	rpl20-rps12	3	ATGAGCCCCTCGAAAGTTTG	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.294731	70063	70860	908-911	1.000000	78.4
rpl20-rps12_p4	rpl20-rps12	4	TCTCCGAGCTACATATCCCC	CGCCGAGGAACATGTACTAG	20	20	55.0	55.0	57.8	57.9	0.0	0.0	0.338626	70063	70860	860-863	1.000000	78.1
rpl20-rps12_p5	rpl20-rps12	5	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.464026	70063	70860	871-874	1.000000	77.1
rps12-psbB_p1	rps12-psbB	1	CTAGTACATGTTCCTCGGCG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.9	58.2	2.3	0.0	0.358409	70975	72193	1346-1358	1.000000	77.8
rps12-psbB_p2	rps12-psbB	2	CTAGTACATGTTCCTCGGCG	ACACGATACCAAGGCAAACC	20	20	55.0	50.0	57.9	58.5	2.3	0.0	0.590793	70975	72193	1349-1361	1.000000	75.9
rps12-psbB_p3	rps12-psbB	3	CTAGTACATGTTCCTCGGCG	TGAACACGATACCAAGGCAA	20	20	55.0	45.0	57.9	57.4	2.3	0.0	0.673715	70975	72193	1352-1364	1.000000	75.3
rps12-psbB_p4	rps12-psbB	4	CTAGTACATGTTCCTCGGCG	GCAACCAGAGCTGTATGCAT	20	20	55.0	50.0	57.9	58.6	2.3	3.8	0.734583	70975	72193	1418-1430	1.000000	74.7
rps12-psbB_p5	rps12-psbB	5	CCTAGTACATGTTCCTCGGC	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.5	58.2	2.3	0.0	0.748060	70975	72193	1347-1359	1.000000	74.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCTGTCGGAAAAAGAAG	AACGCTGTCCAATATCCCTT	21	20	42.9	45.0	57.3	56.9	0.0	0.0	2.792890	111298	112033	920-926	1.000000	58.4
ndhF-rpl32_p2	ndhF-rpl32	2	TCGGAAAAAGAAGAAGTCCCA	AACGCTGTCCAATATCCCTT	21	20	42.9	45.0	57.2	56.9	0.0	0.0	2.951405	111298	112033	912-918	1.000000	57.1
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGCTGTCGGAAAAAGAA	AACGCTGTCCAATATCCCTT	20	20	40.0	45.0	56.1	56.9	0.0	0.0	2.993009	111298	112033	920-926	1.000000	56.7
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCTGTCGGAAAAAGA	AACGCTGTCCAATATCCCTT	20	20	40.0	45.0	56.1	56.9	0.0	0.0	2.993009	111298	112033	921-927	1.000000	56.7
ndhF-rpl32_p5	ndhF-rpl32	5	CGGAAAAAGAAGAAGTCCCAC	AACGCTGTCCAATATCCCTT	21	20	47.6	45.0	57.1	56.9	0.0	0.0	3.000358	111298	112033	911-917	1.000000	56.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGTTGTGCGACAAACAAACA	TCCTAAGAGCAGCGTGTCTA	20	20	40.0	50.0	57.3	57.9	3.7	0.0	0.830832	112196	112938	807-812	1.000000	74.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGTTGTGCGACAAACAAACA	AGCAGCGTGTCTACCAATTT	20	20	40.0	45.0	57.3	57.8	3.7	0.0	0.891559	112196	112938	800-805	1.000000	73.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGTTGTGCGACAAACAAACA	CCTAAGAGCAGCGTGTCTAC	20	20	40.0	55.0	57.3	57.8	3.7	0.0	0.902850	112196	112938	806-811	1.000000	73.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTTGTGCGACAAACAAACA	CTAAGAGCAGCGTGTCTACC	20	20	40.0	55.0	57.3	57.8	3.7	0.0	0.902850	112196	112938	805-810	1.000000	73.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGTTGTGCGACAAACAAACA	GAGCAGCGTGTCTACCAATT	20	20	40.0	50.0	57.3	58.3	3.7	0.0	0.979701	112196	112938	801-806	1.000000	73.2
ndhE-ndhG_p1	ndhE-ndhG	1	GACACATAAGGGCTCTAACCA	AGGTGCAATTGCTGTAGCTC	21	20	47.6	50.0	57.1	58.5	0.0	29.4	2.449374	116744	116956	341-341	1.000000	61.6
ndhE-ndhG_p2	ndhE-ndhG	2	AGTTCAAGACACATAAGGGCTC	AGGTGCAATTGCTGTAGCTC	22	20	45.5	50.0	58.1	58.5	0.0	29.4	2.598567	116744	116956	348-348	1.000000	60.4
ndhE-ndhG_p3	ndhE-ndhG	3	GTTCAAGACACATAAGGGCTCT	AGGTGCAATTGCTGTAGCTC	22	20	45.5	50.0	58.1	58.5	0.0	29.4	2.598567	116744	116956	347-347	1.000000	60.4
ndhE-ndhG_p4	ndhE-ndhG	4	AGTTCAAGACACATAAGGGCT	AGGTGCAATTGCTGTAGCTC	21	20	42.9	50.0	56.9	58.5	0.0	29.4	2.620709	116744	116956	348-348	1.000000	60.2
ndhE-ndhG_p5	ndhE-ndhG	5	TCAAGACACATAAGGGCTCT	AGGTGCAATTGCTGTAGCTC	20	20	45.0	50.0	55.9	58.5	0.0	29.4	2.691720	116744	116956	345-345	1.000000	59.6
ycf1_p1	ycf1	1	CCTGCGAGGGACTTCTTTAC	ATCCGCCAAGTTTGTCAACT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.030284	126375	126494	530-533	1.000000	82.3
ycf1_p2	ycf1	2	ATCCACTCGCCATTCTGTTC	ATCCGCCAAGTTTGTCAACT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048582	126375	126494	596-599	1.000000	82.0
ycf1_p3	ycf1	3	GATCCACTCGCCATTCTGTT	ATCCGCCAAGTTTGTCAACT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048582	126375	126494	597-600	1.000000	82.0
ycf1_p4	ycf1	4	CCTGCGAGGGACTTCTTTAC	ATGCCGTCACCCATTCTTAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.049927	126375	126494	942-945	1.000000	81.7
ycf1_p5	ycf1	5	ATCCACTCGCCATTCTGTTC	ATGCCGTCACCCATTCTTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068225	126375	126494	1008-1011	1.000000	81.5