primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3276	4150	962-978	1.000000	66.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3276	4150	961-977	1.000000	66.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACTCCTTAAAGAGAAGCGGA	GTCGTGCAGTGCAATTCAAT	20	20	45.0	45.0	55.9	57.7	8.1	21.7	2.448754	3276	4150	1052-1068	1.000000	64.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	55.9	57.5	8.1	0.0	2.572956	3276	4150	975-991	1.000000	64.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	20	20	45.0	50.0	55.9	58.5	8.1	0.0	2.607840	3276	4150	978-994	1.000000	63.8
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8563	9355	838-867	1.000000	60.1
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8563	9355	841-870	1.000000	59.0
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8563	9355	840-869	1.000000	59.0
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.2	55.5	0.0	0.0	3.773135	8563	9355	894-923	1.000000	58.2
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8563	9355	839-868	1.000000	58.1
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	57.5	0.0	14.6	0.934514	27404	28579	1244-1263	1.000000	73.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.6	57.5	0.0	14.6	0.960477	27404	28579	1245-1264	1.000000	72.9
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.4	58.8	0.0	0.0	1.174588	27404	28579	1281-1300	1.000000	71.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATTCCGG	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	57.6	58.8	0.0	0.0	1.200551	27404	28579	1282-1301	1.000000	71.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCCCTCATTTCCATTCCGGA	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	58.4	58.8	0.0	0.7	1.244140	27404	28579	1267-1286	1.000000	70.6
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	29853	30700	988-1008	1.000000	70.1
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	29853	30700	983-1003	1.000000	69.2
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	29853	30700	933-953	1.000000	69.0
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	29853	30700	992-1012	1.000000	67.2
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	29853	30700	991-1011	1.000000	66.4
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	36359	36495	244-244	1.000000	81.5
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CTGTTCTTTCCATGACCCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	36359	36495	243-243	1.000000	81.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.188901	36359	36495	283-283	1.000000	81.3
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CTGTTCTTTCCATGACCCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	36359	36495	254-254	1.000000	80.8
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.259146	36359	36495	294-294	1.000000	80.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47530	48582	907-1103	1.000000	84.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47530	48582	915-1111	1.000000	83.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47530	48582	939-1135	1.000000	83.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47530	48582	947-1143	1.000000	82.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47530	48582	930-1126	1.000000	82.6
atpB-rbcL_p1	atpB-rbcL	1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.213422	56844	57613	905-909	1.000000	79.2
atpB-rbcL_p2	atpB-rbcL	2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	57.9	58.1	2.1	0.0	0.255358	56844	57613	883-887	1.000000	78.9
atpB-rbcL_p3	atpB-rbcL	3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.514303	56844	57613	906-910	1.000000	76.8
atpB-rbcL_p4	atpB-rbcL	4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	20	20	45.0	55.0	57.9	58.4	2.1	0.0	0.556239	56844	57613	884-888	1.000000	76.5
atpB-rbcL_p5	atpB-rbcL	5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.5	58.1	8.0	0.0	0.616166	56844	57613	884-888	1.000000	76.0
accD_p1	accD	1	GGATTGAGCCGAACCGAATA	GGTCCCATCGGATCTAGACA	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.237501	59851	61332	2522-2584	1.000000	82.2
accD_p2	accD	2	GGATTGAGCCGAACCGAATA	TGGTCCCATCGGATCTAGAC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.237501	59851	61332	2523-2585	1.000000	82.2
accD_p3	accD	3	GGATTGAGCCGAACCGAATA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	0.0	26.9	0.261303	59851	61332	2468-2530	1.000000	82.1
accD_p4	accD	4	GGATTGAGCCGAACCGAATA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	0.0	26.8	0.337837	59851	61332	2474-2536	1.000000	81.5
accD_p5	accD	5	GGATTGAGCCGAACCGAATA	GTCATAGAATGGGTGCCTCG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.363219	59851	61332	2403-2465	1.000000	81.4
accD-psaI_p1	accD-psaI	1	GACAGTACCCGATGGTTCAC	GGTCCCATCGGATCTAGACA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.009172	61333	61617	498-560	1.000000	87.5
accD-psaI_p2	accD-psaI	2	GACAGTACCCGATGGTTCAC	TGGTCCCATCGGATCTAGAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.009172	61333	61617	499-561	1.000000	87.5
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	GGTCCCATCGGATCTAGACA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027542	61333	61617	387-449	0.666667	71.0
accD-psaI_p4	accD-psaI	4	CCACGGTTTCTTTCCCTTGA	TGGTCCCATCGGATCTAGAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027542	61333	61617	388-450	0.666667	71.0
accD-psaI_p5	accD-psaI	5	TCCACGGTTTCTTTCCCTTG	GGTCCCATCGGATCTAGACA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027542	61333	61617	388-450	0.666667	71.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	66519	67801	1420-1430	1.000000	77.1
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	66519	67801	1418-1428	1.000000	77.0
psbE-petL_p3	psbE-petL	3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.565192	66519	67801	1433-1443	1.000000	76.8
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.569541	66519	67801	1431-1441	1.000000	76.7
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.647263	66519	67801	1429-1439	1.000000	76.1
rpl16_p1	rpl16	1	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACCA	20	26	50.0	30.8	57.9	58.1	0.0	0.0	6.217863	83551	85115	1644-1669	1.000000	43.4
rpl16_p2	rpl16	2	GTCCAGAATAAACCTGCGGT	GGTTGAATAAAAATTCGATTGACCA	20	25	50.0	32.0	57.9	56.9	0.0	0.0	6.223742	83551	85115	1643-1668	1.000000	43.4
rpl16_p3	rpl16	3	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACC	20	25	50.0	32.0	57.9	56.6	0.0	0.0	6.464149	83551	85115	1644-1669	1.000000	43.4
rpl16_p4	rpl16	4	TCCGTCCAGAATAAACCTGC	AGGTTGAATAAAAATTCGATTGACCA	20	26	50.0	30.8	57.6	58.1	0.0	0.0	6.505028	83551	85115	1647-1672	1.000000	43.4
rpl16_p5	rpl16	5	TCCGTCCAGAATAAACCTGC	GGTTGAATAAAAATTCGATTGACCA	20	25	50.0	32.0	57.6	56.9	0.0	0.0	6.510907	83551	85115	1646-1671	1.000000	43.4
rpl22_p1	rpl22	1	GCGAACCAAAAGGAACGATG	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.0	58.0	0.0	9.6	0.053641	85995	86321	702-706	1.000000	82.0
rpl22_p2	rpl22	2	GCGAACCAAAAGGAACGATG	TAGTAACTTGGTCTCGGGCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.108766	85995	86321	841-845	1.000000	81.1
rpl22_p3	rpl22	3	GCGAACCAAAAGGAACGATG	GATCCACTTGGCTACATCCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.165173	85995	86321	1088-1092	1.000000	80.3
rpl22_p4	rpl22	4	GCGAACCAAAAGGAACGATG	CAATCCACTGCCTTGATCCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.169535	85995	86321	1102-1106	1.000000	80.2
rpl22_p5	rpl22	5	GCGAACCAAAAGGAACGATG	AATCCACTGCCTTGATCCAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.171042	85995	86321	1101-1105	1.000000	80.2
rpl22-rps19_p1	rpl22-rps19	1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.5	58.0	8.2	9.6	0.525718	86322	86524	299-303	1.000000	81.4
rpl22-rps19_p2	rpl22-rps19	2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	20	20	50.0	55.0	58.5	57.8	8.2	9.6	0.689756	86322	86524	304-308	1.000000	80.0
rpl22-rps19_p3	rpl22-rps19	3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	20	20	50.0	50.0	58.5	57.7	8.2	9.6	0.778605	86322	86524	300-304	1.000000	79.3
rpl22-rps19_p4	rpl22-rps19	4	CATAGGAACGTCCGCGAATC	TCTGACTTTCGCGAGACATG	20	20	55.0	50.0	58.9	58.0	8.2	9.6	0.888062	86322	86524	307-311	1.000000	78.3
rpl22-rps19_p5	rpl22-rps19	5	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	20	20	50.0	50.0	58.5	58.5	8.2	17.5	0.959344	86322	86524	323-327	1.000000	77.5
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	AAAGAGATGGCTTTTCCCGG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.2	58.0	0.0	15.9	0.168407	95883	96485	745-1171	1.000000	81.5
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	AAAGAGATGGCTTTTCCCGG	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.2	58.0	0.0	15.9	0.169777	95883	96485	747-1173	1.000000	81.5
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	95883	96485	739-1165	1.000000	81.0
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	95883	96485	738-1164	1.000000	81.0
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	95883	96485	740-1166	1.000000	81.0
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGAAAAGGTATTATCCATGCA	TCCCTTTTTCTTCCAAATATTCCT	24	24	37.5	33.3	57.0	56.2	0.0	0.0	10.729019	115259	115535	393-452	1.000000	43.5
ndhF-rpl32_p2	ndhF-rpl32	2	TGGAAGAAAAGGTATTATCCATGC	TCCCTTTTTCTTCCAAATATTCCT	24	24	37.5	33.3	57.0	56.2	0.0	0.0	10.729019	115259	115535	394-453	1.000000	43.4
ndhF-rpl32_p3	ndhF-rpl32	3	GTGGAAGAAAAGGTATTATCCATGC	TCCCTTTTTCTTCCAAATATTCCT	25	24	40.0	33.3	58.3	56.2	0.0	0.0	11.030269	115259	115535	395-454	1.000000	43.4
ndhF-rpl32_p4	ndhF-rpl32	4	CTGTAAGTGGAAGAAAAGGT	TCCCTTTTTCTTCCAAATATTCCT	20	24	40.0	33.3	52.7	56.2	0.0	0.0	11.044341	115259	115535	401-460	1.000000	43.4
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGAAAAGGTATTATCCATGCA	GCAGTTAAATATCCCTTTTTCTTCCA	24	26	37.5	34.6	57.0	58.2	0.0	0.0	11.122797	115259	115535	404-463	1.000000	43.4
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119626	120365	785-934	1.000000	82.4
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119626	120365	784-933	1.000000	82.4
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	119626	120365	805-954	1.000000	82.3
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	119626	120365	783-932	1.000000	81.3
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	119626	120365	760-909	1.000000	81.0
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	AAAGAGATGGCTTTTCCCGG	20	20	50.0	50.0	58.0	58.2	15.9	0.0	0.168407	147171	147773	745-1171	1.000000	81.5
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	AAAGAGATGGCTTTTCCCGG	20	20	50.0	50.0	58.0	58.2	15.9	0.0	0.169777	147171	147773	747-1173	1.000000	81.5
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	147171	147773	739-1165	1.000000	81.0
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	147171	147773	738-1164	1.000000	81.0
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	147171	147773	740-1166	1.000000	81.0