primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psaB-petF_p1	psaB-petF	1	GAGCTTTAGATGCACGTGGA	AGATGCAGCAGAAGAAGCAG	20	20	50.0	50.0	58.0	58.3	18.9	4.1	0.269848	4727	5908	1995-1995	0.500000	57.8
psaB-petF_p2	psaB-petF	2	AGGAGCTTTAGATGCACGTG	AGATGCAGCAGAAGAAGCAG	20	20	50.0	50.0	58.0	58.3	12.3	4.1	0.275439	4727	5908	1997-1997	0.500000	57.8
psaB-petF_p3	psaB-petF	3	GGCACATGAACGTACTCCAT	AGATGCAGCAGAAGAAGCAG	20	20	50.0	50.0	58.0	58.3	0.0	4.1	0.283879	4727	5908	1565-1565	0.500000	57.7
psaB-petF_p4	psaB-petF	4	AAAAATCGGCCCAGGTGATT	AGATGCAGCAGAAGAAGCAG	20	20	45.0	50.0	58.1	58.3	0.0	4.1	0.322512	4727	5908	2075-2075	0.500000	57.4
psaB-petF_p5	psaB-petF	5	AAAATCGGCCCAGGTGATTT	AGATGCAGCAGAAGAAGCAG	20	20	45.0	50.0	58.1	58.3	9.1	4.1	0.322512	4727	5908	2074-2074	0.500000	57.4
sufB_p1	sufB	1	TTACTGTGTACGCAGCATCC	CAATTGGATCGACTTCGGGT	20	20	50.0	50.0	58.0	58.0	31.3	0.0	0.028758	11109	12566	2223-2223	0.500000	59.8
sufB_p2	sufB	2	TTACTGTGTACGCAGCATCC	CCAAATGCGAACGTTGTTCA	20	20	50.0	45.0	58.0	57.9	31.3	5.0	0.117057	11109	12566	2103-2103	0.500000	59.1
sufB_p3	sufB	3	CGTTTGGCACTGGATCTACA	CAATTGGATCGACTTCGGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117482	11109	12566	2406-2406	0.500000	59.1
sufB_p4	sufB	4	TTTGGCACTGGATCTACACG	CAATTGGATCGACTTCGGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117482	11109	12566	2404-2404	0.500000	59.1
sufB_p5	sufB	5	TTACTGTGTACGCAGCATCC	CACCCGAAAACCCTTCGTTA	20	20	50.0	50.0	58.0	58.1	31.3	6.2	0.122992	11109	12566	2313-2313	0.500000	59.0
sufC_p1	sufC	1	ACTCGAAGTCGCATTGTCTC	GCCTCCGCTAAGAATGTGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040203	12566	13321	1320-1320	0.500000	59.7
sufC_p2	sufC	2	CTCGAAGTCGCATTGTCTCA	GCCTCCGCTAAGAATGTGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041563	12566	13321	1319-1319	0.500000	59.7
sufC_p3	sufC	3	CTTATGCGCAGGGGATTCTT	GCCTCCGCTAAGAATGTGAA	20	20	50.0	50.0	58.0	58.0	18.3	0.0	0.051304	12566	13321	1507-1507	0.500000	59.6
sufC_p4	sufC	4	TTATGCGCAGGGGATTCTTC	GCCTCCGCTAAGAATGTGAA	20	20	50.0	50.0	58.0	58.0	18.3	0.0	0.056857	12566	13321	1506-1506	0.500000	59.5
sufC_p5	sufC	5	GCGCAGGGGATTCTTCTAAA	GCCTCCGCTAAGAATGTGAA	20	20	50.0	50.0	58.0	58.0	0.3	0.0	0.062562	12566	13321	1502-1502	0.500000	59.5
bas1_p1	bas1	1	CAAGCAGAAGGTCACAGGTT	TAAGGCTGCTTTTCGGGTTT	20	20	50.0	45.0	58.0	58.0	0.0	1.3	0.037422	21223	21858	1255-1255	0.500000	59.7
bas1_p2	bas1	2	CAAGCAGAAGGTCACAGGTT	TTAAGGCTGCTTTTCGGGTT	20	20	50.0	45.0	58.0	58.0	0.0	1.3	0.037422	21223	21858	1256-1256	0.500000	59.7
bas1_p3	bas1	3	CAAGCAGAAGGTCACAGGTT	TTTAAGGCTGCTTTTCGGGT	20	20	50.0	45.0	58.0	58.0	0.0	1.3	0.037422	21223	21858	1257-1257	0.500000	59.7
bas1_p4	bas1	4	GCAGAAGGTCACAGGTTCAA	TAAGGCTGCTTTTCGGGTTT	20	20	50.0	45.0	58.0	58.0	0.0	1.3	0.042975	21223	21858	1252-1252	0.500000	59.7
bas1_p5	bas1	5	GCAGAAGGTCACAGGTTCAA	TTAAGGCTGCTTTTCGGGTT	20	20	50.0	45.0	58.0	58.0	0.0	1.3	0.042975	21223	21858	1253-1253	0.500000	59.7
rpoB-rps20_p1	rpoB-rps20	1	AGGAATAACCAGTTGTGCCG	AGAAACACCAGCAGAACGTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.217170	39768	40255	1321-1321	0.500000	58.3
rpoB-rps20_p2	rpoB-rps20	2	GGAATAACCAGTTGTGCCGA	AGAAACACCAGCAGAACGTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.222312	39768	40255	1320-1320	0.500000	58.2
rpoB-rps20_p3	rpoB-rps20	3	TGCCGAGTAATTTGCGGTAT	AGAAACACCAGCAGAACGTT	20	20	45.0	45.0	57.7	58.0	12.8	0.0	0.357523	39768	40255	1306-1306	0.500000	57.1
rpoB-rps20_p4	rpoB-rps20	4	GTTGTGCCGAGTAATTTGCG	AGAAACACCAGCAGAACGTT	20	20	50.0	45.0	58.4	58.0	0.0	0.0	0.436196	39768	40255	1310-1310	0.500000	56.5
rpoB-rps20_p5	rpoB-rps20	5	ACCAGTTGTGCCGAGTAATT	AGAAACACCAGCAGAACGTT	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.599865	39768	40255	1314-1314	0.500000	55.2
trnS-GCU-trnD-GUC_p1	trnS-GCU-trnD-GUC	1	AATGGCTGAGTGGTCGAAAG	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.239769	50641	50713	217-218	1.000000	86.9
trnS-GCU-trnD-GUC_p2	trnS-GCU-trnD-GUC	2	AATGGCTGAGTGGTCGAAAG	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	58.2	57.9	0.0	4.0	0.239769	50641	50713	218-219	1.000000	86.9
trnS-GCU-trnD-GUC_p3	trnS-GCU-trnD-GUC	3	GAAATGGCTGAGTGGTCGAA	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.244871	50641	50713	219-220	1.000000	86.8
trnS-GCU-trnD-GUC_p4	trnS-GCU-trnD-GUC	4	GAAATGGCTGAGTGGTCGAA	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	58.2	57.9	0.0	4.0	0.244871	50641	50713	220-221	1.000000	86.7
trnS-GCU-trnD-GUC_p5	trnS-GCU-trnD-GUC	5	GGAGAAATGGCTGAGTGGTC	AGACTCGAACTCGCAACTTC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.309286	50641	50713	222-223	1.000000	86.2
ycf90_p1	ycf90	1	TTTGGTTTGGTATCGGTGCA	TTTAGTAGGTGGTTTCGCGG	20	20	45.0	50.0	58.0	57.9	0.0	6.1	0.100506	58790	60211	1869-1869	0.500000	59.2
ycf90_p2	ycf90	2	TTTGGTTTGGTATCGGTGCA	ATGGCAAACACTGGTCGAAT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.107640	58790	60211	1907-1907	0.500000	59.1
ycf90_p3	ycf90	3	GGGTTAGCAGTAATGGCTCC	TTTAGTAGGTGGTTTCGCGG	20	20	55.0	50.0	58.0	57.9	0.0	6.1	0.114462	58790	60211	2134-2134	0.500000	59.1
ycf90_p4	ycf90	4	GGGTTAGCAGTAATGGCTCC	ATGGCAAACACTGGTCGAAT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.121596	58790	60211	2172-2172	0.500000	59.0
ycf90_p5	ycf90	5	GGCTCCTACTCAAATGGGTG	TTTAGTAGGTGGTTTCGCGG	20	20	55.0	50.0	58.0	57.9	0.0	6.1	0.127289	58790	60211	2120-2120	0.500000	59.0
ycf39_p1	ycf39	1	AATTTTCGATAGCCGGGGTC	CAAGGTCACGACGATTAGGA	20	20	50.0	50.0	58.0	57.1	2.2	0.0	0.970323	63443	64432	1676-1676	0.500000	52.2
ycf39_p2	ycf39	2	AATTTTCGATAGCCGGGGTC	TCAAGGTCACGACGATTAGG	20	20	50.0	50.0	58.0	57.1	2.2	0.0	0.970323	63443	64432	1677-1677	0.500000	52.2
ycf39_p3	ycf39	3	GAATTTTCGATAGCCGGGGT	CAAGGTCACGACGATTAGGA	20	20	50.0	50.0	58.0	57.1	2.2	0.0	0.970323	63443	64432	1677-1677	0.500000	52.2
ycf39_p4	ycf39	4	GAATTTTCGATAGCCGGGGT	TCAAGGTCACGACGATTAGG	20	20	50.0	50.0	58.0	57.1	2.2	0.0	0.970323	63443	64432	1678-1678	0.500000	52.2
ycf39_p5	ycf39	5	AATTTTCGATAGCCGGGGTC	CGATTAGGATTTCGTGACGGA	20	21	50.0	47.6	58.0	57.9	2.2	4.7	1.111332	63443	64432	1665-1665	0.500000	51.1
trnP-UGG-ycf89_p1	trnP-UGG-ycf89	1	GAAGATGGAGTCATGCAGCA	ATTCCAGGAGCATCGGGATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.064132	66197	66546	1099-1099	0.500000	59.5
trnP-UGG-ycf89_p2	trnP-UGG-ycf89	2	GATGGAGTCATGCAGCAGAA	ATTCCAGGAGCATCGGGATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.064132	66197	66546	1096-1096	0.500000	59.5
trnP-UGG-ycf89_p3	trnP-UGG-ycf89	3	AAGATGGAGTCATGCAGCAG	ATTCCAGGAGCATCGGGATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.069845	66197	66546	1098-1098	0.500000	59.4
trnP-UGG-ycf89_p4	trnP-UGG-ycf89	4	GAAGATGGAGTCATGCAGCA	GCAAAGACCCATGGATACGT	20	20	50.0	50.0	58.0	58.0	0.0	15.2	0.072491	66197	66546	1513-1513	0.500000	59.4
trnP-UGG-ycf89_p5	trnP-UGG-ycf89	5	GATGGAGTCATGCAGCAGAA	GCAAAGACCCATGGATACGT	20	20	50.0	50.0	58.0	58.0	0.0	15.2	0.072491	66197	66546	1510-1510	0.500000	59.4
dnaK_p1	dnaK	1	GCAATTGTCGCACCATTGAA	GGACCCCTTGCAAAATGGTA	20	20	45.0	50.0	57.9	58.1	7.4	12.7	0.131520	88331	90178	2892-2892	0.500000	58.9
dnaK_p2	dnaK	2	CGAAGGTGTAGTTGATCCGG	GGACCCCTTGCAAAATGGTA	20	20	55.0	50.0	58.1	58.1	0.0	12.7	0.145794	88331	90178	2758-2758	0.500000	58.8
dnaK_p3	dnaK	3	GCCCGTTGTGAACAATTACG	TAAGTGCTTCATGTGGAGCC	20	20	50.0	50.0	58.0	57.9	0.0	10.6	0.155443	88331	90178	2585-2585	0.500000	58.8
dnaK_p4	dnaK	4	GCAATTGTCGCACCATTGAA	TAAGTGCTTCATGTGGAGCC	20	20	45.0	50.0	57.9	57.9	7.4	10.6	0.177016	88331	90178	2273-2273	0.500000	58.6
dnaK_p5	dnaK	5	CGAAGGTGTAGTTGATCCGG	TAAGTGCTTCATGTGGAGCC	20	20	55.0	50.0	58.1	57.9	0.0	10.6	0.191290	88331	90178	2139-2139	0.500000	58.5
dnaK-rpl3_p1	dnaK-rpl3	1	TGTTGTTCCTAAATCAATTCCAACT	TGGGTCATTCCAATTTTGTTACC	25	23	32.0	39.1	57.4	57.5	0.0	0.0	9.071910	90179	90500	399-400	1.000000	51.0
dnaK-rpl3_p2	dnaK-rpl3	2	TGTTGTTCCTAAATCAATTCCAACT	GGGTCATTCCAATTTTGTTACCT	25	23	32.0	39.1	57.4	57.3	0.0	0.0	9.334438	90179	90500	398-399	1.000000	51.0
dnaK-rpl3_p3	dnaK-rpl3	3	TGTTGTTCCTAAATCAATTCCAAC	TGGGTCATTCCAATTTTGTTACC	24	23	33.3	39.1	56.1	57.5	0.0	0.0	9.372201	90179	90500	399-400	1.000000	51.0
dnaK-rpl3_p4	dnaK-rpl3	4	GCAACTACAGAATTTGTTGT	TGGGTCATTCCAATTTTGTTACC	20	23	35.0	39.1	52.3	57.5	0.0	0.0	9.182686	90179	90500	413-414	1.000000	50.9
dnaK-rpl3_p5	dnaK-rpl3	5	GCAACTACAGAATTTGTTGT	GGGTCATTCCAATTTTGTTACCT	20	23	35.0	39.1	52.3	57.3	0.0	0.0	9.445214	90179	90500	412-413	1.000000	50.9
rpl2_p1	rpl2	1	GGTGGGGTGATATTTGGACC	ATAGTCGAGCTACGTGACCA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.101470	92148	92975	1699-1699	0.500000	59.2
rpl2_p2	rpl2	2	GGTGGGGTGATATTTGGACC	GAGCTACGTGACCAAGTTGT	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.217763	92148	92975	1693-1693	0.500000	58.3
rpl2_p3	rpl2	3	GGTGGGGTGATATTTGGACC	TAGTCGAGCTACGTGACCAA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.255130	92148	92975	1698-1698	0.500000	58.0
rpl2_p4	rpl2	4	GGTGGTGGGGTGATATTTGG	ATAGTCGAGCTACGTGACCA	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.258719	92148	92975	1702-1702	0.500000	57.9
rpl2_p5	rpl2	5	GGTGGTGGGGTGATATTTGG	GAGCTACGTGACCAAGTTGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.375012	92148	92975	1696-1696	0.500000	57.0
rps3_p1	rps3	1	CATTCTGTAGCAGCAAACGC	TTACCACGCATTCTTCCTCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.117263	94169	94816	970-970	0.500000	59.1
rps3_p2	rps3	2	CTGTAGCAGCAAACGCAAAA	TTACCACGCATTCTTCCTCG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.122811	94169	94816	966-966	0.500000	59.0
rps3_p3	rps3	3	CATTCTGTAGCAGCAAACGC	TAATCGTTCGTCGTGTTGCT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.167367	94169	94816	1074-1074	0.500000	58.7
rps3_p4	rps3	4	CTGTAGCAGCAAACGCAAAA	TAATCGTTCGTCGTGTTGCT	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.172916	94169	94816	1070-1070	0.500000	58.6
rps3_p5	rps3	5	CATTCTGTAGCAGCAAACGC	CTGTCGCAACCCAATAGTCA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.205986	94169	94816	1200-1200	0.500000	58.4
rpl6_p1	rpl6	1	ACACTTACGCCGTAAACGTT	AACAACAACAGCACGGAAAC	20	20	45.0	45.0	58.1	57.7	11.3	0.0	0.420163	97639	98181	1646-1646	0.500000	56.6
rpl6_p2	rpl6	2	ACACTTACGCCGTAAACGTT	TGGCCGTTCAAATGTACCTT	20	20	45.0	45.0	58.1	57.7	11.3	0.0	0.428276	97639	98181	1185-1185	0.500000	56.6
rpl6_p3	rpl6	3	TGGGGTTGGTGGTGAAATTT	AACAACAACAGCACGGAAAC	20	20	45.0	45.0	57.8	57.7	0.0	0.0	0.454337	97639	98181	1216-1216	0.500000	56.4
rpl6_p4	rpl6	4	TTGGGGTTGGTGGTGAAATT	AACAACAACAGCACGGAAAC	20	20	45.0	45.0	57.8	57.7	0.0	0.0	0.454337	97639	98181	1217-1217	0.500000	56.4
rpl6_p5	rpl6	5	TGGGGTTGGTGGTGAAATTT	TGGCCGTTCAAATGTACCTT	20	20	45.0	45.0	57.8	57.7	0.0	0.0	0.462450	97639	98181	755-755	0.500000	56.3
rpoA_p1	rpoA	1	TCGTCTGGTAGTTCTGGGTT	TCGTTTCCCGACACCAATTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.035278	101499	102461	1746-1746	0.500000	59.7
rpoA_p2	rpoA	2	TCGTCTGGTAGTTCTGGGTT	TTTGACCTGTTAAACCGCCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.059602	101499	102461	1939-1939	0.500000	59.5
rpoA_p3	rpoA	3	TCGTCTGGTAGTTCTGGGTT	GATCTTGTGCTACATGCGGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.065396	101499	102461	1657-1657	0.500000	59.5
rpoA_p4	rpoA	4	ACAATATCTTGGGCGTCGTC	TCGTTTCCCGACACCAATTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.093976	101499	102461	1761-1761	0.500000	59.2
rpoA_p5	rpoA	5	ACAATATCTTGGGCGTCGTC	TTTGACCTGTTAAACCGCCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.118300	101499	102461	1954-1954	0.500000	59.1
clpC_p1	clpC	1	GGCACAAACGAAACGTGAAT	AGGTGTACAGTCAGGACGAT	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.331421	106711	109383	2969-2969	0.500000	57.3
clpC_p2	clpC	2	GGCACAAACGAAACGTGAAT	AGCTGGTCGAATGGAAAAGA	20	20	45.0	45.0	57.9	57.1	0.0	0.0	0.973076	106711	109383	2939-2939	0.500000	52.2
clpC_p3	clpC	3	GGCACAAACGAAACGTGAAT	TAGGTGTACAGTCAGGACGA	20	20	45.0	50.0	57.9	57.1	0.0	0.0	0.979030	106711	109383	2970-2970	0.500000	52.2
clpC_p4	clpC	4	GGCACAAACGAAACGTGAAT	GGTGTACAGTCAGGACGATT	20	20	45.0	50.0	57.9	57.0	0.0	0.0	1.146125	106711	109383	2968-2968	0.500000	50.8
clpC_p5	clpC	5	GGCACAAACGAAACGTGAAT	AGCTGGTCGAATGGAAAAGAA	20	21	45.0	42.9	57.9	57.9	0.0	0.0	1.254235	106711	109383	2939-2939	0.500000	50.0
ycf89_p1	ycf89	1	TCTCGTACGACTTGCATGTG	AGGATTTGAACCTAGGGCAC	20	20	50.0	50.0	58.0	57.2	15.3	14.8	0.831718	127743	128705	1724-1734	1.000000	83.0
ycf89_p2	ycf89	2	CATTGCGGAAAATTCCCCAC	AGGATTTGAACCTAGGGCAC	20	20	50.0	50.0	58.0	57.2	6.6	14.8	0.817658	127743	128705	2000-2011	1.000000	82.9
ycf89_p3	ycf89	3	TATTGCTCCGTCAGGCTTTC	AGGATTTGAACCTAGGGCAC	20	20	50.0	50.0	58.0	57.2	0.0	14.8	0.833082	127743	128705	2023-2034	1.000000	82.8
ycf89_p4	ycf89	4	ATTGCGGAAAATTCCCCACT	AGGATTTGAACCTAGGGCAC	20	20	45.0	50.0	58.1	57.2	6.6	14.8	0.871715	127743	128705	1999-2010	1.000000	82.5
ycf89_p5	ycf89	5	CAACCCATAGGCAGTCATCC	AGGATTTGAACCTAGGGCAC	20	20	55.0	50.0	58.0	57.2	0.0	14.8	0.836034	127743	128705	2063-2063	0.500000	53.3