primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-AAA_p1	psbA-trnK-AAA	1	ACGGTTTTCAGTGCTGGTTA	AAAATGCAAGCACGGTTTGG	20	20	45.0	45.0	57.7	58.4	0.0	0.0	0.754500	1466	1744	449-470	1.000000	79.9
psbA-trnK-AAA_p2	psbA-trnK-AAA	2	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	0.0	0.0	0.944750	1466	1744	379-402	1.000000	78.7
psbA-trnK-AAA_p3	psbA-trnK-AAA	3	ACGGTTTTCAGTGCTGGTTA	TGAAAAATGCAAGCACGGTT	20	20	45.0	40.0	57.7	57.4	0.0	0.0	0.928662	1466	1744	452-473	1.000000	78.5
psbA-trnK-AAA_p4	psbA-trnK-AAA	4	TACAGAAGCGACCCCATAGG	AAAATGCAAGCACGGTTTGG	20	20	55.0	45.0	58.6	58.4	0.0	0.0	1.009180	1466	1744	423-444	1.000000	78.0
psbA-trnK-AAA_p5	psbA-trnK-AAA	5	ATCCAGTTACAGAAGCGACC	AAAATGCAAGCACGGTTTGG	20	20	50.0	45.0	57.3	58.4	0.0	0.0	1.093980	1466	1744	430-451	1.000000	77.2
trnK-AAA-rps16_p1	trnK-AAA-rps16	1	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4381	5026	981-1045	1.000000	84.4
trnK-AAA-rps16_p2	trnK-AAA-rps16	2	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4381	5026	982-1046	1.000000	84.4
trnK-AAA-rps16_p3	trnK-AAA-rps16	3	CCTTTCAGGATCAGTCGTGG	ATGCGTGAATCAAGCGTTTG	20	20	55.0	45.0	58.0	58.0	0.0	15.7	0.047974	4381	5026	1486-1571	1.000000	83.9
trnK-AAA-rps16_p4	trnK-AAA-rps16	4	CCTTTCAGGATCAGTCGTGG	CATGCGTGAATCAAGCGTTT	20	20	55.0	45.0	58.0	58.0	0.0	12.9	0.047974	4381	5026	1487-1572	1.000000	83.9
trnK-AAA-rps16_p5	trnK-AAA-rps16	5	TCAGGATCAGTCGTGGTCTT	ATGCGTGAATCAAGCGTTTG	20	20	50.0	45.0	58.1	58.0	0.0	15.7	0.107507	4381	5026	1482-1567	1.000000	83.4
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	20	22	50.0	31.8	57.9	54.4	16.6	7.7	5.620197	7115	7507	411-446	1.000000	48.3
psbK-psbI_p2	psbK-psbI	2	TGGCAAGCAGCTGTAAGTTT	ACAAAAAGTTTGAGAGTAAGCA	20	22	45.0	31.8	58.3	54.4	16.6	7.7	5.872906	7115	7507	408-443	1.000000	48.3
psbK-psbI_p3	psbK-psbI	3	TTGGCAAGCAGCTGTAAGTT	ACAAAAAGTTTGAGAGTAAGCA	20	22	45.0	31.8	58.3	54.4	16.6	7.7	5.872906	7115	7507	409-444	1.000000	48.3
psbK-psbI_p4	psbK-psbI	4	TTTGGCAAGCAGCTGTAAGT	ACAAAAAGTTTGAGAGTAAGCA	20	22	45.0	31.8	58.3	54.4	16.6	7.7	5.872906	7115	7507	410-445	1.000000	48.3
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCAT	20	23	50.0	30.4	57.9	55.0	16.6	7.7	6.053342	7115	7507	411-446	1.000000	48.3
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	GTTTGGCAAGCAGCTGTAAG	TGGTTTCATTCGGCTCCTTT	20	20	50.0	45.0	57.9	57.7	16.6	0.0	0.342459	7802	8394	1984-2021	1.000000	83.1
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	GTTTGGCAAGCAGCTGTAAG	TTGGTTTCATTCGGCTCCTT	20	20	50.0	45.0	57.9	57.7	16.6	0.0	0.342459	7802	8394	1985-2022	1.000000	83.1
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	GTTTGGCAAGCAGCTGTAAG	TTTGGTTTCATTCGGCTCCT	20	20	50.0	45.0	57.9	57.7	16.6	0.0	0.342459	7802	8394	1986-2023	1.000000	83.1
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	CCAACGCTTTAGTCCACTCA	TGGTTTCATTCGGCTCCTTT	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.450183	7802	8394	1298-1298	0.500000	56.4
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	CCAACGCTTTAGTCCACTCA	TTGGTTTCATTCGGCTCCTT	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.450183	7802	8394	1299-1299	0.500000	56.4
atpF-atpH_p1	atpF-atpH	1	GAAAGAATCGGTTAAATTTTTCA	TGGCCTGGTTGTAGCATTAG	23	20	26.1	50.0	52.3	57.6	0.0	3.3	9.140753	12614	13108	565-568	1.000000	45.2
atpF-atpH_p2	atpF-atpH	2	GAAAGAATCGGTTAAATTTTTCAT	TGGCCTGGTTGTAGCATTAG	24	20	25.0	50.0	52.9	57.6	0.0	3.3	9.521617	12614	13108	565-568	1.000000	45.2
atpF-atpH_p3	atpF-atpH	3	GAAAGAATCGGTTAAATTTTTCA	TTTATGGCCTGGTTGTAGCA	23	20	26.1	45.0	52.3	57.1	0.0	3.3	9.611554	12614	13108	569-572	1.000000	45.2
atpF-atpH_p4	atpF-atpH	4	GAAAGAATCGGTTAAATTTTTCA	GGCCTGGTTGTAGCATTAGC	23	20	26.1	55.0	52.3	59.0	0.0	0.0	9.697344	12614	13108	564-567	1.000000	45.2
atpF-atpH_p5	atpF-atpH	5	GAAAGAATCGGTTAAATTTTTCA	ATGGCCTGGTTGTAGCATTAG	23	21	26.1	47.6	52.3	58.1	0.0	3.3	9.786182	12614	13108	566-569	1.000000	45.2
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	58.0	58.0	0.0	5.7	0.054014	13355	13833	624-625	1.000000	84.1
atpH-atpI_p2	atpH-atpI	2	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	20	20	45.0	50.0	58.1	58.0	0.0	5.7	0.127956	13355	13833	588-589	1.000000	83.7
atpH-atpI_p3	atpH-atpI	3	AATAGAAGCAAGCCCGACAG	TTTTTGCCACTTTAGCTGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	13355	13833	601-602	1.000000	83.4
atpH-atpI_p4	atpH-atpI	4	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.136455	13355	13833	646-647	1.000000	83.2
atpH-atpI_p5	atpH-atpI	5	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	20	20	55.0	50.0	58.2	58.0	0.0	5.7	0.214492	13355	13833	650-651	1.000000	82.6
trnS-UCC-rps4_p1	trnS-UCC-rps4	1	GAGTGGTTGAAGGCGTAACA	AGGAAATGCGTCGGTTTGAA	20	20	50.0	45.0	58.1	58.4	0.0	0.0	0.526279	44250	44524	416-421	1.000000	78.8
trnS-UCC-rps4_p2	trnS-UCC-rps4	2	GAGTGGTTGAAGGCGTAACA	GGAAATGCGTCGGTTTGAAA	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.535455	44250	44524	415-420	1.000000	78.8
trnS-UCC-rps4_p3	trnS-UCC-rps4	3	GAGTGGTTGAAGGCGTAACA	TAGGAAATGCGTCGGTTTGA	20	20	50.0	45.0	58.1	57.5	0.0	0.0	0.585363	44250	44524	417-422	1.000000	78.4
trnS-UCC-rps4_p4	trnS-UCC-rps4	4	TCCTGATGTCGGAGAGATGG	AGGAAATGCGTCGGTTTGAA	20	20	55.0	45.0	58.3	58.4	0.0	0.0	0.702693	44250	44524	438-443	1.000000	77.3
trnS-UCC-rps4_p5	trnS-UCC-rps4	5	TCCTGATGTCGGAGAGATGG	GGAAATGCGTCGGTTTGAAA	20	20	55.0	45.0	58.3	57.6	0.0	0.0	0.711869	44250	44524	437-442	1.000000	77.2
trnL-UUA-trnF-UUC_p1	trnL-UUA-trnF-UUC	1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	46786	47282	587-794	1.000000	85.4
trnL-UUA-trnF-UUC_p2	trnL-UUA-trnF-UUC	2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	46786	47282	584-791	1.000000	82.1
trnL-UUA-trnF-UUC_p3	trnL-UUA-trnF-UUC	3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.0	58.1	0.0	20.1	1.013432	46786	47282	576-783	1.000000	81.5
trnL-UUA-trnF-UUC_p4	trnL-UUA-trnF-UUC	4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	20	20	50.0	55.0	57.5	58.6	0.0	0.0	1.064405	46786	47282	549-756	1.000000	81.4
trnL-UUA-trnF-UUC_p5	trnL-UUA-trnF-UUC	5	AATCGTGAGGGTTCAAGTCC	TGAACTGGTGACACGAGGAT	20	20	50.0	50.0	57.5	58.7	0.0	20.1	1.136303	46786	47282	585-792	1.000000	80.5
trnF-UUC-ndhJ_p1	trnF-UUC-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47356	47925	675-682	1.000000	86.5
trnF-UUC-ndhJ_p2	trnF-UUC-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47356	47925	674-681	1.000000	86.5
trnF-UUC-ndhJ_p3	trnF-UUC-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.062041	47356	47925	702-709	1.000000	86.4
trnF-UUC-ndhJ_p4	trnF-UUC-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.132182	47356	47925	699-706	1.000000	85.9
trnF-UUC-ndhJ_p5	trnF-UUC-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.276511	47356	47925	709-716	1.000000	84.6
accD_p1	accD	1	TTTAAGTTCCGTGTCAGGGC	CCGGAAAGACTAGGCCTACT	20	20	50.0	55.0	58.1	58.2	6.7	8.9	0.335173	56109	57566	2632-2649	1.000000	80.8
accD_p2	accD	2	CCATCCTGCATGTTGTCCTT	CCGGAAAGACTAGGCCTACT	20	20	50.0	55.0	58.2	58.2	0.0	8.9	0.378691	56109	57566	2584-2601	1.000000	80.5
accD_p3	accD	3	GCATCGATTGCGTACTCTGA	CCGGAAAGACTAGGCCTACT	20	20	50.0	55.0	58.2	58.2	0.0	8.9	0.381879	56109	57566	2328-2346	1.000000	80.5
accD_p4	accD	4	TTTAAGTTCCGTGTCAGGGC	CAAGTGCCGGAAAGACTAGG	20	20	50.0	55.0	58.1	58.3	6.7	5.0	0.386260	56109	57566	2638-2655	1.000000	80.4
accD_p5	accD	5	TTTAAGTTCCGTGTCAGGGC	CGGGAAATGCACAAAGATGG	20	20	50.0	50.0	58.1	57.7	6.7	0.0	0.395814	56109	57566	2162-2167	1.000000	80.0
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.0	58.1	0.0	0.0	1.132033	61602	62213	800-806	1.000000	74.4
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.0	57.8	0.0	0.0	1.223633	61602	62213	801-807	1.000000	73.7
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.2	58.1	13.8	0.0	1.272430	61602	62213	797-803	1.000000	73.3
petA-psbJ_p4	petA-psbJ	4	CATTACGTGTCCAAGGCCTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.2	57.8	13.8	0.0	1.364030	61602	62213	798-804	1.000000	72.6
petA-psbJ_p5	petA-psbJ	5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCTTGTGATCGGT	20	21	45.0	47.6	57.6	58.1	17.6	0.0	1.445352	61602	62213	795-801	1.000000	71.9
ndhF-rpl32_p1	ndhF-rpl32	1	GCAATAGGTCGTGTGAACCA	ACGAATACGCTTTTTCGAGA	20	20	50.0	40.0	58.2	55.5	0.0	19.5	2.650043	111001	111833	1393-1393	0.500000	38.8
ndhF-rpl32_p2	ndhF-rpl32	2	GGTCGTGTGAACCAAAAACC	ACGAATACGCTTTTTCGAGA	20	20	50.0	40.0	57.8	55.5	0.0	19.5	2.651169	111001	111833	1387-1387	0.500000	38.8
ndhF-rpl32_p3	ndhF-rpl32	3	GCAATAGGTCGTGTGAACCA	ACTTAGTGTGGCTTATTGTGGA	20	22	50.0	40.9	58.2	57.4	0.0	0.0	2.830678	111001	111833	1903-1903	0.500000	37.4
ndhF-rpl32_p4	ndhF-rpl32	4	GGTCGTGTGAACCAAAAACC	ACTTAGTGTGGCTTATTGTGGA	20	22	50.0	40.9	57.8	57.4	0.0	0.0	2.831804	111001	111833	1897-1897	0.500000	37.3
ndhF-rpl32_p5	ndhF-rpl32	5	GCAATAGGTCGTGTGAACCA	CACTTAGTGTGGCTTATTGTGG	20	22	50.0	45.5	58.2	57.3	0.0	3.9	2.881835	111001	111833	1904-1904	0.500000	36.9
rpl32-trnL-CUA_p1	rpl32-trnL-CUA	1	TCGTTGAAAGCTTTTTCGTTAGG	TCCTAAGAGCAGCGTGTCTA	23	20	39.1	50.0	58.2	57.9	14.0	0.0	3.284482	111993	112952	952-1077	1.000000	63.1
rpl32-trnL-CUA_p2	rpl32-trnL-CUA	2	GTTGAAAGCTTTTTCGTTAGGGA	TCCTAAGAGCAGCGTGTCTA	23	20	39.1	50.0	57.8	57.9	14.0	0.0	3.302003	111993	112952	950-1075	1.000000	63.0
rpl32-trnL-CUA_p3	rpl32-trnL-CUA	3	CGTTGAAAGCTTTTTCGTTAGG	TCCTAAGAGCAGCGTGTCTA	22	20	40.9	50.0	56.8	57.9	14.0	0.0	3.308951	111993	112952	951-1076	1.000000	62.9
rpl32-trnL-CUA_p4	rpl32-trnL-CUA	4	TCGTTGAAAGCTTTTTCGTTAGG	AGCAGCGTGTCTACCAATTT	23	20	39.1	45.0	58.2	57.8	14.0	0.0	3.345209	111993	112952	945-1070	1.000000	62.6
rpl32-trnL-CUA_p5	rpl32-trnL-CUA	5	TCGTTGAAAGCTTTTTCGTTAGG	CTAAGAGCAGCGTGTCTACC	23	20	39.1	55.0	58.2	57.8	14.0	0.0	3.356499	111993	112952	950-1075	1.000000	62.5
trnL-CUA-ccsA_p1	trnL-CUA-ccsA	1	TTTGAACAGGTTGCCGCTAT	TGCAGACTGAGGCATTTTGT	20	20	45.0	45.0	58.1	58.0	0.0	6.7	0.110240	113033	113150	589-594	1.000000	81.1
trnL-CUA-ccsA_p2	trnL-CUA-ccsA	2	TAGACACGCTGCTCTTAGGA	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	57.9	58.0	0.0	6.7	0.138804	113033	113150	558-563	1.000000	80.9
trnL-CUA-ccsA_p3	trnL-CUA-ccsA	3	TTTGAACAGGTTGCCGCTAT	TAACCACTGGGACTGGAGAG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.157129	113033	113150	622-627	1.000000	80.7
trnL-CUA-ccsA_p4	trnL-CUA-ccsA	4	TAGACACGCTGCTCTTAGGA	TAACCACTGGGACTGGAGAG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.185693	113033	113150	591-596	1.000000	80.4
trnL-CUA-ccsA_p5	trnL-CUA-ccsA	5	AAATTGGTAGACACGCTGCT	TGCAGACTGAGGCATTTTGT	20	20	45.0	45.0	57.8	58.0	0.0	6.7	0.199531	113033	113150	565-570	1.000000	80.4
rps15-ycf1_p1	rps15-ycf1	1	CAGCAGTCGTTGACGTTTTC	GGACAAATTCTTCACTCAGCG	20	21	50.0	47.6	58.0	57.5	3.2	0.0	1.467135	122237	124502	2823-2825	1.000000	71.8
rps15-ycf1_p2	rps15-ycf1	2	GATCCCCTGCTTTCGTCTTT	GGACAAATTCTTCACTCAGCG	20	21	50.0	47.6	57.9	57.5	0.0	0.0	1.577289	122237	124502	2693-2695	1.000000	71.0
rps15-ycf1_p3	rps15-ycf1	3	ATAAGCCAGCAGTCGTTGAC	GGACAAATTCTTCACTCAGCG	20	21	50.0	47.6	58.3	57.5	0.0	0.0	1.735093	122237	124502	2829-2831	1.000000	69.6
rps15-ycf1_p4	rps15-ycf1	4	TCGTTGACGTTTTCCCAGAA	GGACAAATTCTTCACTCAGCG	20	21	45.0	47.6	57.7	57.5	3.2	0.0	1.774669	122237	124502	2817-2819	1.000000	69.3
rps15-ycf1_p5	rps15-ycf1	5	ACAAATAAGCCAGCAGTCGT	GGACAAATTCTTCACTCAGCG	20	21	45.0	47.6	57.8	57.5	0.0	0.0	1.646951	122237	124502	2833-2833	0.500000	46.8
ycf1_p1	ycf1	1	GTTTTACGCCTTTGGGAACG	TGATCCATTTTTGCACGGGA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.124209	126025	126144	627-627	1.000000	83.3
ycf1_p2	ycf1	2	GAGTGCGCTTTTGCTTGAAA	TGATCCATTTTTGCACGGGA	20	20	45.0	45.0	58.2	58.1	9.7	0.0	0.244581	126025	126144	663-663	1.000000	82.3
ycf1_p3	ycf1	3	GACCACTCGGGAAGTTGAAA	TGATCCATTTTTGCACGGGA	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.333338	126025	126144	981-981	1.000000	81.5
ycf1_p4	ycf1	4	AGTGCGCTTTTGCTTGAAAA	TGATCCATTTTTGCACGGGA	20	20	40.0	45.0	57.7	58.1	9.7	0.0	0.375597	126025	126144	662-662	1.000000	81.2
ycf1_p5	ycf1	5	GCCTTTGGGAACGCATAGAT	TGATCCATTTTTGCACGGGA	20	20	50.0	45.0	58.3	58.1	9.9	0.0	0.403157	126025	126144	620-620	0.500000	56.8