primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	rps7-ndhB	1	TGATGCAGCTAGAGATAATGGG	AGATGCATTCTGGTAAAATAGT	22	22	45.5	31.8	57.5	53.2	2.5	0.0	9.335358	1429	1630	356-357	1.000000	47.2
rps7-ndhB_p2	rps7-ndhB	2	TGCAGCTAGAGATAATGGGA	AGATGCATTCTGGTAAAATAGT	20	22	45.0	31.8	55.4	53.2	0.0	0.0	9.385579	1429	1630	353-354	1.000000	47.2
rps7-ndhB_p3	rps7-ndhB	3	TGATGCAGCTAGAGATAATGGG	AAGATGCATTCTGGTAAAATAGT	22	23	45.5	30.4	57.5	54.1	2.5	0.0	9.497253	1429	1630	357-358	1.000000	47.2
rps7-ndhB_p4	rps7-ndhB	4	TGCAGCTAGAGATAATGGGA	AAGATGCATTCTGGTAAAATAGT	20	23	45.0	30.4	55.4	54.1	0.0	0.0	9.547474	1429	1630	354-355	1.000000	47.2
rps7-ndhB_p5	rps7-ndhB	5	GATGCAGCTAGAGATAATGGGA	AGATGCATTCTGGTAAAATAGT	22	22	45.5	31.8	57.2	53.2	0.0	0.0	9.602439	1429	1630	355-356	1.000000	47.2
ndhB_p1	ndhB	1	GGAGCAGCAAGAAAACGTTC	CGCGTGTTTACCATTTCACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.079524	1631	3649	2489-2515	1.000000	81.8
ndhB_p2	ndhB	2	GGAAAAGCGTTAGCGATTCG	CGCGTGTTTACCATTTCACC	20	20	50.0	50.0	57.9	58.0	2.2	0.0	0.114573	1631	3649	2519-2545	1.000000	81.5
ndhB_p3	ndhB	3	GGAGCAGCAAGAAAACGTTC	GACTCGAACCTCCATGCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.138545	1631	3649	2529-2555	1.000000	81.3
ndhB_p4	ndhB	4	GGAAAAGCGTTAGCGATTCG	GACTCGAACCTCCATGCTTT	20	20	50.0	50.0	57.9	57.9	2.2	0.0	0.173594	1631	3649	2559-2585	1.000000	80.9
ndhB_p5	ndhB	5	GCAGCAAGAAAACGTTCAGG	CGCGTGTTTACCATTTCACC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.282800	1631	3649	2486-2512	1.000000	80.1
atpI-atpH_p1	atpI-atpH	1	TTTTTGCTACACTTGCAGCA	GCAGCAATAACAGAAGCAGC	20	20	40.0	50.0	56.8	58.1	0.0	0.0	1.338648	17640	18052	507-510	1.000000	74.2
atpI-atpH_p2	atpI-atpH	2	TTTTTGCTACACTTGCAGCA	AAGCAGCAGAAATCAAGGGG	20	20	40.0	50.0	56.8	58.4	0.0	0.0	1.690391	17640	18052	494-497	1.000000	71.5
atpI-atpH_p3	atpI-atpH	3	TTTTTGCTACACTTGCAGCA	CCAGCAGCAATAACAGAAGC	20	20	40.0	50.0	56.8	57.4	0.0	0.0	1.800632	17640	18052	510-513	1.000000	70.4
atpI-atpH_p4	atpI-atpH	4	ATTTTTGCTACACTTGCAGCA	GCAGCAATAACAGAAGCAGC	21	20	38.1	50.0	57.3	58.1	0.0	0.0	1.826202	17640	18052	508-511	1.000000	70.3
atpI-atpH_p5	atpI-atpH	5	TTTTTGCTACACTTGCAGCA	GCAGCAGAAATCAAGGGGTT	20	20	40.0	50.0	56.8	58.7	0.0	0.0	1.983553	17640	18052	492-495	1.000000	69.2
trnR-UCU-trnG-GCC_p1	trnR-UCU-trnG-GCC	1	CCTCTGTCCTATCCTTTAGACG	ACCCTTAGCCTTCCAAGCTA	22	20	50.0	50.0	57.2	58.0	0.0	0.0	2.872823	21306	21387	161-162	1.000000	59.5
trnR-UCU-trnG-GCC_p2	trnR-UCU-trnG-GCC	2	CCTCTGTCCTATCCTTTAGACG	TTCCAAGCTAATGATGCGGG	22	20	50.0	50.0	57.2	58.3	0.0	0.0	3.160529	21306	21387	151-152	1.000000	57.4
trnR-UCU-trnG-GCC_p3	trnR-UCU-trnG-GCC	3	GTCCTATCCTTTAGACGATGGAC	ACCCTTAGCCTTCCAAGCTA	23	20	47.8	50.0	57.9	58.0	0.0	0.0	3.156960	21306	21387	156-157	1.000000	57.3
trnR-UCU-trnG-GCC_p4	trnR-UCU-trnG-GCC	4	CTGTCCTATCCTTTAGACGATGG	ACCCTTAGCCTTCCAAGCTA	23	20	47.8	50.0	57.9	58.0	0.0	0.0	3.160848	21306	21387	158-159	1.000000	57.2
trnR-UCU-trnG-GCC_p5	trnR-UCU-trnG-GCC	5	TGTCCTATCCTTTAGACGATGGA	ACCCTTAGCCTTCCAAGCTA	23	20	43.5	50.0	58.2	58.0	0.0	0.0	3.233132	21306	21387	157-158	1.000000	56.7
psaM-trnS-GCU_p1	psaM-trnS-GCU	1	TGACCTCAATTTCAGATAGTCA	AACCCTCGGTACAAAAAGCT	22	20	36.4	45.0	54.6	57.3	0.0	0.0	6.091384	22640	23022	551-551	1.000000	48.1
psaM-trnS-GCU_p2	psaM-trnS-GCU	2	TGACCTCAATTTCAGATAGTCA	ACCCTCGGTACAAAAAGCTT	22	20	36.4	45.0	54.6	57.3	0.0	0.0	6.091384	22640	23022	550-550	1.000000	48.1
psaM-trnS-GCU_p3	psaM-trnS-GCU	3	TGACCTCAATTTCAGATAGTCA	CGGAGAGAGAGGGATTCGAA	22	20	36.4	55.0	54.6	58.3	0.0	0.0	5.758465	22640	23022	569-569	1.000000	47.9
psaM-trnS-GCU_p4	psaM-trnS-GCU	4	TGACCTCAATTTCAGATAGTCAA	CGGAGAGAGAGGGATTCGAA	23	20	34.8	55.0	55.3	58.3	0.0	0.0	5.980849	22640	23022	569-569	1.000000	47.9
psaM-trnS-GCU_p5	psaM-trnS-GCU	5	TGACCTCAATTTCAGATAGTCA	AACGGAGAGAGAGGGATTCG	22	20	36.4	55.0	54.6	58.6	0.0	0.0	6.045384	22640	23022	571-571	1.000000	47.8
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	GCTCCTGCTGTAAACGGTTA	CTGCCTTAATCCACTTGGCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.223739	29622	30060	475-487	1.000000	86.2
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	GCTCCTGCTGTAAACGGTTA	GATCCACAATCCACTGCCTT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.223739	29622	30060	488-500	1.000000	86.0
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	GCTCCTGCTGTAAACGGTTA	ACTGCCTTAATCCACTTGGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.249526	29622	30060	476-488	1.000000	86.0
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	GCTCCTGCTGTAAACGGTTA	TTATATTGGGCGAACGACGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.233436	29622	30060	529-541	1.000000	85.3
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	GTAACGCTCACAACTTCCCT	CTGCCTTAATCCACTTGGCT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.300108	29622	30060	516-528	1.000000	85.0
trnH-GUG-ycf2_p1	trnH-GUG-ycf2	1	AAGGCAGTGGATTGTGGATC	AACTCCATTGTGTCCATGGA	20	20	50.0	45.0	57.9	56.7	0.0	11.4	1.408149	30134	30468	491-494	1.000000	75.4
trnH-GUG-ycf2_p2	trnH-GUG-ycf2	2	GCCAAGTGGATTAAGGCAGT	AACTCCATTGTGTCCATGGA	20	20	50.0	45.0	58.2	56.7	0.2	11.4	1.433937	30134	30468	503-506	1.000000	75.0
trnH-GUG-ycf2_p3	trnH-GUG-ycf2	3	GATTGTGGATCCTCTACGCG	AACTCCATTGTGTCCATGGA	20	20	55.0	45.0	58.2	56.7	14.2	11.4	1.501178	30134	30468	482-485	1.000000	74.8
trnH-GUG-ycf2_p4	trnH-GUG-ycf2	4	AAGGCAGTGGATTGTGGATC	ACTCCATTGTGTCCATGGAT	20	20	50.0	45.0	57.9	56.5	0.0	19.6	1.643151	30134	30468	490-493	1.000000	73.5
trnH-GUG-ycf2_p5	trnH-GUG-ycf2	5	AAGGCAGTGGATTGTGGATC	AAACTCCATTGTGTCCATGGA	20	21	50.0	42.9	57.9	57.5	0.0	11.4	1.658884	30134	30468	492-495	1.000000	73.4
ycf2_p1	ycf2	1	TGATTTCTTGAATTCTCAGTTGA	CTTCGCGAAGGGTCTGTTAA	23	20	30.4	50.0	54.2	57.9	7.1	35.6	6.849975	33496	33645	1392-1494	1.000000	45.9
ycf2_p2	ycf2	2	TGATTTCTTGAATTCTCAGTTGAA	CTTCGCGAAGGGTCTGTTAA	24	20	29.2	50.0	55.0	57.9	7.1	35.6	7.095031	33496	33645	1392-1494	1.000000	45.9
ycf2_p3	ycf2	3	ATGATTTCTTGAATTCTCAGTTGA	CTTCGCGAAGGGTCTGTTAA	24	20	29.2	50.0	54.8	57.9	7.1	35.6	7.294945	33496	33645	1393-1495	1.000000	45.9
ycf2_p4	ycf2	4	TGATTTCTTGAATTCTCAGTTGA	TTCGCGAAGGGTCTGTTAAA	23	20	30.4	45.0	54.2	57.5	7.1	19.4	7.305208	33496	33645	1391-1493	1.000000	45.9
ycf2_p5	ycf2	5	TGATTTCTTGAATTCTCAGTTG	CTTCGCGAAGGGTCTGTTAA	22	20	31.8	50.0	52.7	57.9	7.1	35.6	7.366039	33496	33645	1392-1494	1.000000	45.9
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	CTCCTGAAGGTTGGTCAAGT	GGGAATCGAACCCGCATATT	20	20	50.0	50.0	57.1	58.1	0.0	13.9	1.046746	42646	42769	289-291	1.000000	77.4
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	CTCCTGAAGGTTGGTCAAGT	CGAACCCGCATATTCTCCTT	20	20	50.0	50.0	57.1	57.8	0.0	0.0	1.179449	42646	42769	283-285	1.000000	76.5
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	CTCCTGAAGGTTGGTCAAGT	CCGCATATTCTCCTTGGCAA	20	20	50.0	50.0	57.1	58.3	0.0	0.0	1.259817	42646	42769	278-280	1.000000	75.9
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	CTCCTGAAGGTTGGTCAAGT	AACCCGCATATTCTCCTTGG	20	20	50.0	50.0	57.1	57.7	0.0	0.0	1.287366	42646	42769	281-283	1.000000	75.7
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TCTCCTGAAGGTTGGTCAAG	GGGAATCGAACCCGCATATT	20	20	50.0	50.0	56.8	58.1	0.0	13.9	1.338272	42646	42769	290-292	1.000000	75.1
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	TACCAGACTGCTCTACTCCG	CGTGAAATGGCTCATGCATG	20	20	55.0	50.0	58.0	58.2	0.0	21.1	0.204697	42970	43069	172-176	1.000000	83.4
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	AAGGTTATGAGCCTTGCGAG	CGTGAAATGGCTCATGCATG	20	20	50.0	50.0	58.0	58.2	0.0	21.1	0.194717	42970	43069	193-197	1.000000	83.0
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	GGATTCGAACCTGTGACCTC	TTGCCTGGAGTCACGAAATC	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.203129	42970	43069	189-193	1.000000	83.0
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	GGATTCGAACCTGTGACCTC	CGTGAAATGGCTCATGCATG	20	20	55.0	50.0	58.0	58.2	0.0	21.1	0.176419	42970	43069	213-217	1.000000	82.7
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	GGATTCGAACCTGTGACCTC	CTTCGTGAAATGGCTCATGC	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.195925	42970	43069	216-220	1.000000	82.5
rps14_p1	rps14	1	GGATTCGAACCTGTGACCTC	GCTCATGAGCGTACTCCTTT	20	20	55.0	50.0	58.0	57.7	0.0	30.6	0.313117	43070	43372	713-722	1.000000	79.8
rps14_p2	rps14	2	AAGGTTATGAGCCTTGCGAG	GCTCATGAGCGTACTCCTTT	20	20	50.0	50.0	58.0	57.7	0.0	30.6	0.331415	43070	43372	693-702	1.000000	79.8
rps14_p3	rps14	3	GGATTCGAACCTGTGACCTC	TCTGTTTGGGCATGGATGTT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.319036	43070	43372	824-833	1.000000	79.6
rps14_p4	rps14	4	GGATTCGAACCTGTGACCTC	TTCTGTTTGGGCATGGATGT	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.319036	43070	43372	825-834	1.000000	79.6
rps14_p5	rps14	5	AAGGTTATGAGCCTTGCGAG	TCTGTTTGGGCATGGATGTT	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.337334	43070	43372	804-813	1.000000	79.5
trnS-UGA-rps4_p1	trnS-UGA-rps4	1	TGGTTGAGTGGTTTAAGGCA	TGTAGAATATTACTCTCGTCAAGT	20	24	45.0	33.3	57.3	54.9	0.0	0.0	7.813096	49714	49939	334-390	1.000000	43.8
trnS-UGA-rps4_p2	trnS-UGA-rps4	2	TGGTTGAGTGGTTTAAGGCA	AGTTGTAGAATATTACTCTCGTCAAGT	20	27	45.0	33.3	57.3	58.0	0.0	0.0	7.780080	49714	49939	337-393	1.000000	43.7
trnS-UGA-rps4_p3	trnS-UGA-rps4	3	TGGTTGAGTGGTTTAAGGCA	AGTTGTAGAATATTACTCTCGTCA	20	24	45.0	33.3	57.3	54.9	0.0	0.0	7.813096	49714	49939	337-393	1.000000	43.7
trnS-UGA-rps4_p4	trnS-UGA-rps4	4	TGGTTGAGTGGTTTAAGGCA	GTTGTAGAATATTACTCTCGTCAAGT	20	26	45.0	34.6	57.3	56.9	0.0	0.0	7.875280	49714	49939	336-392	1.000000	43.7
trnS-UGA-rps4_p5	trnS-UGA-rps4	5	TGGTTGAGTGGTTTAAGGCA	AGTTGTAGAATATTACTCTCGTCAAG	20	26	45.0	34.6	57.3	56.6	0.0	0.0	8.104866	49714	49939	337-393	1.000000	43.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GACCTCTGAGCTAAACAGGC	TTTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	58.0	58.0	0.0	15.0	0.030440	50828	51028	259-260	1.000000	83.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GACCTCTGAGCTAAACAGGC	AATTTAAGTCCGCAGCGTCT	20	20	55.0	45.0	58.0	57.9	0.0	15.0	0.111900	50828	51028	261-262	1.000000	82.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GAGTCGAACCGATGACCATC	TTTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	58.2	58.0	0.0	15.0	0.171852	50828	51028	300-301	1.000000	81.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	AATTTAAGTCCGCAGCGTCT	20	20	55.0	45.0	58.2	57.9	0.0	15.0	0.253313	50828	51028	302-303	1.000000	80.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GACCTCTGAGCTAAACAGGC	TACCAATTTCGCCATACCCC	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.368844	50828	51028	242-243	1.000000	80.7
psaJ-rpl33_p1	psaJ-rpl33	1	ACTGCACCTGTTTTAGCTACA	TTTTCATCATTTTGAGCACAA	21	21	42.9	28.6	57.5	52.8	0.0	0.0	7.699665	65641	65754	287-287	1.000000	42.0
psaJ-rpl33_p2	psaJ-rpl33	2	ACTGCACCTGTTTTAGCTACA	TGAGCACAATTAATACACTCT	21	21	42.9	33.3	57.5	52.6	0.0	0.0	7.904367	65641	65754	275-275	1.000000	42.0
psaJ-rpl33_p3	psaJ-rpl33	3	ACTGCACCTGTTTTAGCTACA	TTCATCATTTTGAGCACAATT	21	21	42.9	28.6	57.5	52.6	0.0	0.0	7.933996	65641	65754	285-285	1.000000	42.0
psaJ-rpl33_p4	psaJ-rpl33	4	ACTGCACCTGTTTTAGCTACA	TTTTTCATCATTTTGAGCACA	21	21	42.9	28.6	57.5	52.8	0.0	0.0	7.699665	65641	65754	288-288	1.000000	41.9
psaJ-rpl33_p5	psaJ-rpl33	5	ACTGCACCTGTTTTAGCTACA	TTTTTCATCATTTTGAGCACAA	21	22	42.9	27.3	57.5	53.7	0.0	0.0	7.819636	65641	65754	288-288	1.000000	41.9
rpl16-rps3_p1	rpl16-rps3	1	AACGAGTCACACACTAAGCA	AGTTTGGATATTTCAAGATGAAT	20	23	45.0	26.1	57.1	52.1	0.0	0.0	9.779934	77989	78051	100-112	1.000000	47.8
rpl16-rps3_p2	rpl16-rps3	2	ACGAGTCACACACTAAGCAT	AAGTTTGGATATTTCAAGATGAA	20	23	45.0	26.1	56.9	52.3	0.0	0.0	9.786662	77989	78051	100-112	1.000000	47.8
rpl16-rps3_p3	rpl16-rps3	3	AACGAGTCACACACTAAGCA	AAAGTTTGGATATTTCAAGATGA	20	23	45.0	26.1	57.1	52.3	0.0	0.0	9.560375	77989	78051	102-114	1.000000	47.7
rpl16-rps3_p4	rpl16-rps3	4	AACGAGTCACACACTAAGCA	AAGTTTGGATATTTCAAGATGAA	20	23	45.0	26.1	57.1	52.3	0.0	0.0	9.560375	77989	78051	101-113	1.000000	47.7
rpl16-rps3_p5	rpl16-rps3	5	AACGAGTCACACACTAAGCA	AAAGTTTGGATATTTCAAGATGAA	20	24	45.0	25.0	57.1	53.2	0.0	0.0	9.719606	77989	78051	102-114	1.000000	47.7
cysT-trnL-UAG_p1	cysT-trnL-UAG	1	TGAACAATATGATTATCAAAGTGCT	AGCAGCGTGTCTACCAATTT	25	20	28.0	45.0	55.4	57.8	0.0	0.0	7.813381	95054	95301	391-395	1.000000	45.7
cysT-trnL-UAG_p2	cysT-trnL-UAG	2	TGAACAATATGATTATCAAAGTGCT	TCCTAAGAGCAGCGTGTCTA	25	20	28.0	50.0	55.4	57.9	0.0	0.0	7.752654	95054	95301	398-402	1.000000	45.6
cysT-trnL-UAG_p3	cysT-trnL-UAG	3	TGAACAATATGATTATCAAAGTGCT	CTAAGAGCAGCGTGTCTACC	25	20	28.0	55.0	55.4	57.8	0.0	0.0	7.824672	95054	95301	396-400	1.000000	45.6
cysT-trnL-UAG_p4	cysT-trnL-UAG	4	TGAACAATATGATTATCAAAGTGCT	TCGAACCGAGATGCATTAGC	25	20	28.0	50.0	55.4	58.1	0.0	0.0	7.775435	95054	95301	424-428	1.000000	45.3
cysT-trnL-UAG_p5	cysT-trnL-UAG	5	TGAACAATATGATTATCAAAGTGCT	GGATTCGAACCGAGATGCAT	25	20	28.0	50.0	55.4	58.1	0.0	0.0	7.757297	95054	95301	428-432	1.000000	45.2
ycf1-chlN_p1	ycf1-chlN	1	AACCCATAGCACAGAAAGCA	ACTTGAACTTGTTACACGTCCT	20	22	45.0	40.9	57.4	57.9	0.0	0.0	2.717848	108614	108760	268-269	1.000000	62.4
ycf1-chlN_p2	ycf1-chlN	2	ACCCATAGCACAGAAAGCAA	ACTTGAACTTGTTACACGTCCT	20	22	45.0	40.9	57.4	57.9	0.0	0.0	2.717848	108614	108760	267-268	1.000000	62.4
ycf1-chlN_p3	ycf1-chlN	3	AACCCATAGCACAGAAAGCA	ACTTGAACTTGTTACACGTCC	20	21	45.0	42.9	57.4	56.4	0.0	0.0	3.158992	108614	108760	268-269	1.000000	58.9
ycf1-chlN_p4	ycf1-chlN	4	ACCCATAGCACAGAAAGCAA	ACTTGAACTTGTTACACGTCC	20	21	45.0	42.9	57.4	56.4	0.0	0.0	3.158992	108614	108760	267-268	1.000000	58.9
ycf1-chlN_p5	ycf1-chlN	5	TGGAACCCATAGCACAGAAAG	ACTTGAACTTGTTACACGTCCT	21	22	47.6	40.9	57.9	57.9	0.0	0.0	3.204887	108614	108760	271-272	1.000000	58.5