primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.0	56.5	0.0	13.9	1.591763	5018	5733	835-842	1.000000	70.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	5018	5733	791-798	1.000000	69.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	5018	5733	790-797	1.000000	69.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCCTCGCTTCATCCAAATGT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	0.0	13.9	1.793898	5018	5733	834-841	1.000000	68.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCTAGCCGCACTTAAAAGC	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.3	56.5	0.0	13.9	1.814915	5018	5733	806-813	1.000000	68.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGCTTTCTACCACAGCGTTT	AAATATAGATGGGGCGTGGC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.238579	6824	8070	857-1366	1.000000	82.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTGCTTTCTACCACAGCGTT	AAATATAGATGGGGCGTGGC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.238579	6824	8070	858-1367	1.000000	82.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AAATATAGATGGGGCGTGGC	20	20	45.0	50.0	58.1	57.8	8.1	0.0	0.339697	6824	8070	868-1377	1.000000	81.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	AAATATAGATGGGGCGTGGC	20	20	55.0	50.0	58.4	57.8	7.8	0.0	0.570225	6824	8070	883-1392	1.000000	80.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	AAATATAGATGGGGCGTGGC	20	20	55.0	50.0	58.4	57.8	2.9	0.0	0.570225	6824	8070	882-1391	1.000000	80.0
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.368120	13807	14104	413-479	1.000000	80.1
atpF-atpH_p2	atpF-atpH	2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.8	58.2	3.3	0.0	0.428719	13807	14104	386-452	1.000000	79.8
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	3.3	0.536681	13807	14104	415-481	1.000000	78.7
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531623	13807	14104	484-550	1.000000	78.3
atpF-atpH_p5	atpF-atpH	5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	20	20	50.0	50.0	57.8	57.6	3.3	0.0	0.592222	13807	14104	457-523	1.000000	78.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.189918	27888	29105	1122-1372	1.000000	83.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.195241	27888	29105	1123-1373	1.000000	83.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.433374	27888	29105	1140-1390	1.000000	81.8
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.438696	27888	29105	1141-1391	1.000000	81.7
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.1	58.4	0.0	14.6	0.463281	27888	29105	1104-1354	1.000000	81.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	32351	33120	824-854	1.000000	82.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	20	20	60.0	50.0	58.2	57.9	0.0	0.0	0.317890	32351	33120	859-889	1.000000	81.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGTGTAGTACCCTACCCCCA	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.706875	32351	33120	860-890	1.000000	78.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	TGACTTACGCCTTACCATGG	20	20	50.0	50.0	57.8	57.3	0.0	11.2	0.853834	32351	33120	852-882	1.000000	77.6
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	GCCTTACCATGGCGTTACTC	20	20	50.0	55.0	57.8	58.7	0.0	13.0	0.873105	32351	33120	844-874	1.000000	77.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	46337	47161	906-921	1.000000	73.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	46337	47161	927-942	1.000000	72.8
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	46337	47161	877-892	1.000000	72.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	46337	47161	879-894	1.000000	72.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	46337	47161	907-922	1.000000	70.9
accD-psaI_p1	accD-psaI	1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	58.8	57.9	0.0	0.0	0.864190	60865	61305	556-567	1.000000	76.5
accD-psaI_p2	accD-psaI	2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	20	20	50.0	45.0	58.8	58.2	0.0	7.5	0.914646	60865	61305	546-557	1.000000	76.2
accD-psaI_p3	accD-psaI	3	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	19	20	52.6	45.0	58.1	57.9	0.0	0.0	1.169454	60865	61305	556-567	1.000000	74.1
accD-psaI_p4	accD-psaI	4	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	21	20	42.9	45.0	58.1	57.9	0.0	0.0	1.177813	60865	61305	554-565	1.000000	74.0
accD-psaI_p5	accD-psaI	5	GAGTTATTTCAGCTCCACGC	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	56.9	57.9	0.0	0.0	1.199152	60865	61305	567-578	1.000000	73.8
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	64515	65483	1159-1178	1.000000	74.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	64515	65483	1146-1165	1.000000	74.5
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	64515	65483	1160-1179	1.000000	74.0
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	64515	65483	1147-1166	1.000000	73.7
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	64515	65483	1164-1183	1.000000	73.1
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATTTCCCCGT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.0	58.7	0.0	9.8	0.736204	70178	70963	828-850	1.000000	77.7
rpl20-rps12_p2	rpl20-rps12	2	TCCGAGCTATATTTCCCCGT	AGCGTCGAGGAACATGTACT	20	20	50.0	50.0	58.0	58.8	0.0	9.8	0.830587	70178	70963	827-849	1.000000	76.9
rpl20-rps12_p3	rpl20-rps12	3	TCCGAGCTATATTTCCCCGT	TCAGCGTCGAGGAACATGTA	20	20	50.0	50.0	58.0	58.8	0.0	9.8	0.834928	70178	70963	829-851	1.000000	76.9
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATATTTCCCCGT	GCGTCGAGGAACATGTACTA	20	20	50.0	50.0	58.0	57.2	0.0	9.8	0.843756	70178	70963	826-848	1.000000	76.8
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	70178	70963	882-904	1.000000	76.6
ycf2_p1	ycf2	1	TACCTAGCGACAAACTCCCA	GTTCCAGCTCCGTATGAAGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.131188	92148	92267	178-268	1.000000	87.3
ycf2_p2	ycf2	2	TACCTAGCGACAAACTCCCA	CAGCTCCGTATGAAGGTCAC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.144618	92148	92267	174-264	1.000000	87.3
ycf2_p3	ycf2	3	CGACAAACTCCCATGTTCCT	GTTCCAGCTCCGTATGAAGG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.243679	92148	92267	171-261	1.000000	86.6
ycf2_p4	ycf2	4	CGACAAACTCCCATGTTCCT	CAGCTCCGTATGAAGGTCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.257109	92148	92267	167-257	1.000000	86.6
ycf2_p5	ycf2	5	TACCTAGCGACAAACTCCCA	TAGCAGTTCCAGCTCCGTAT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.295851	92148	92267	183-273	1.000000	85.9
ycf1-ndhF_p1	ycf1-ndhF	1	ACCGAATTGGAATTCCTGTCT	GTGGGCGCATCTCTTCTTAT	21	20	42.9	50.0	57.3	57.8	24.7	10.8	1.967806	111835	111873	142-180	1.000000	72.4
ycf1-ndhF_p2	ycf1-ndhF	2	ACCGAATTGGAATTCCTGTCT	TGGGCGCATCTCTTCTTATC	21	20	42.9	50.0	57.3	57.5	24.7	10.8	2.254353	111835	111873	141-179	1.000000	70.1
ycf1-ndhF_p3	ycf1-ndhF	3	ACCGAATTGGAATTCCTGTC	GTGGGCGCATCTCTTCTTAT	20	20	45.0	50.0	55.7	57.8	24.7	10.8	2.521665	111835	111873	142-180	1.000000	68.0
ycf1-ndhF_p4	ycf1-ndhF	4	ACCGAATTGGAATTCCTGTCT	AGTGGGCGCATCTCTTCTTA	21	20	42.9	50.0	57.3	58.8	24.7	10.8	2.525973	111835	111873	143-181	1.000000	67.9
ycf1-ndhF_p5	ycf1-ndhF	5	ACCGAATTGGAATTCCTGTCT	TAGTGGGCGCATCTCTTCTT	21	20	42.9	50.0	57.3	58.8	24.7	10.8	2.525973	111835	111873	144-182	1.000000	67.9
ndhF-rpl32_p1	ndhF-rpl32	1	TGCCGACGGAAAAAGAAGAA	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	58.0	55.3	20.5	0.0	6.763201	114121	115176	1181-1221	1.000000	45.2
ndhF-rpl32_p2	ndhF-rpl32	2	AAAAGAAGAAGTCCCGCTCC	TCCAAATATTTTTACGAATACGCT	20	24	50.0	29.2	57.8	55.3	0.0	0.0	6.906030	114121	115176	1171-1211	1.000000	45.2
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGACGGAAAAAGAAG	TCCAAATATTTTTACGAATACGCT	20	24	50.0	29.2	58.0	55.3	20.5	0.0	6.751912	114121	115176	1183-1223	0.909091	41.0
ndhF-rpl32_p4	ndhF-rpl32	4	TTGCCGACGGAAAAAGAAGA	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	58.0	55.3	20.5	0.0	6.763201	114121	115176	1182-1222	0.909091	41.0
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGACGGAAAAAG	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	57.8	55.3	20.5	0.0	6.915793	114121	115176	1186-1226	0.909091	41.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	115339	116314	1011-1100	1.000000	84.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	115339	116314	1012-1101	1.000000	84.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	115339	116314	1032-1121	1.000000	84.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	115339	116314	1034-1123	1.000000	84.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	115339	116314	1021-1110	1.000000	83.6
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	TGGACCGCGAGAGTTATTTG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.012413	116452	117465	1498-1505	1.000000	81.9
ccsA_p2	ccsA	2	AACCGACAAAAGCCTGGAAT	TGGACCGCGAGAGTTATTTG	20	20	45.0	50.0	58.0	58.0	0.0	8.2	0.009206	116452	117465	1918-1925	1.000000	81.8
ccsA_p3	ccsA	3	ACCGACAAAAGCCTGGAATT	TGGACCGCGAGAGTTATTTG	20	20	45.0	50.0	58.0	58.0	0.0	8.2	0.009206	116452	117465	1917-1924	1.000000	81.8
ccsA_p4	ccsA	4	AACCGACAAAAGCCTGGAAT	GGACCGCGAGAGTTATTTGT	20	20	45.0	50.0	58.0	58.0	0.0	8.2	0.010594	116452	117465	1917-1924	1.000000	81.8
ccsA_p5	ccsA	5	ACCGACAAAAGCCTGGAATT	GGACCGCGAGAGTTATTTGT	20	20	45.0	50.0	58.0	58.0	0.0	8.2	0.010594	116452	117465	1916-1923	1.000000	81.8
ccsA-ndhD_p1	ccsA-ndhD	1	GGCGTCTATGGGCTTTCTTA	CAGTTGACAAGGTCGAGGTT	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.855322	117466	117688	373-380	1.000000	77.6
ccsA-ndhD_p2	ccsA-ndhD	2	GGCGTCTATGGGCTTTCTTA	AGTTGACAAGGTCGAGGTTC	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.142870	117466	117688	372-379	1.000000	75.4
ccsA-ndhD_p3	ccsA-ndhD	3	GGCGTCTATGGGCTTTCTTA	GTTGACAAGGTCGAGGTTCT	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.142870	117466	117688	371-378	1.000000	75.4
ccsA-ndhD_p4	ccsA-ndhD	4	GGCGTCTATGGGCTTTCTTA	TCAGTTGACAAGGTCGAGGT	20	20	50.0	50.0	57.4	58.6	0.0	0.0	1.204824	117466	117688	374-381	1.000000	74.8
ccsA-ndhD_p5	ccsA-ndhD	5	GGCGTCTATGGGCTTTCTTA	ATCAGTTGACAAGGTCGAGG	20	20	50.0	50.0	57.4	57.2	0.0	0.0	1.365995	117466	117688	375-382	1.000000	73.5
ndhA_p1	ndhA	1	TGTTTAAGACAAGTCGTGAAGA	CACCCGGTTTTATCAATTTGCA	22	22	36.4	40.9	55.7	58.1	0.0	0.0	6.423329	121847	124009	2322-2332	1.000000	41.2
ndhA_p2	ndhA	2	TGTTTAAGACAAGTCGTGAAGA	ACCCGGTTTTATCAATTTGCA	22	21	36.4	38.1	55.7	56.7	0.0	0.0	6.659529	121847	124009	2321-2331	1.000000	41.2
ndhA_p3	ndhA	3	TTGTTTAAGACAAGTCGTGAAGA	CACCCGGTTTTATCAATTTGCA	23	22	34.8	40.9	56.4	58.1	0.0	0.0	6.708456	121847	124009	2323-2333	1.000000	41.2
ndhA_p4	ndhA	4	TGTTTAAGACAAGTCGTGAAGA	ACCACCCGGTTTTATCAATTTG	22	22	36.4	40.9	55.7	57.5	0.0	0.0	6.871654	121847	124009	2324-2334	1.000000	41.2
ndhA_p5	ndhA	5	AGACAAGTCGTGAAGATAGA	CACCCGGTTTTATCAATTTGCA	20	22	40.0	40.9	53.2	58.1	0.0	0.0	6.907836	121847	124009	2316-2326	1.000000	41.2