primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	AATGATCCCGGACGAAATCC	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.7	58.2	0.7	17.2	0.473046	7712	7811	157-159	1.000000	84.8
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	AATGATCCCGGACGAAATCC	AGAGGTCTAAAGCGTCGGAT	20	20	50.0	50.0	57.7	58.2	0.7	0.0	0.490887	7712	7811	209-211	1.000000	82.5
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	AATGATCCCGGACGAAATCC	AGGTCTAAAGCGTCGGATTG	20	20	50.0	50.0	57.7	57.7	0.7	0.0	0.551370	7712	7811	207-209	1.000000	82.1
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	AATGATCCCGGACGAAATCC	GTCTAAAGCGTCGGATTGCT	20	20	50.0	50.0	57.7	58.4	0.7	2.3	0.614540	7712	7811	205-207	1.000000	81.7
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	AATGATCCCGGACGAAATCC	GAGGTCTAAAGCGTCGGATT	20	20	50.0	50.0	57.7	57.4	0.7	0.0	0.837083	7712	7811	208-210	1.000000	79.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	TACTGATGCGGGTTCGATTC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	57.8	56.9	0.0	0.0	2.270558	9708	9898	258-259	1.000000	69.6
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CTACTGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	57.8	56.9	0.0	0.0	2.276673	9708	9898	259-260	1.000000	69.5
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	TACTGATGCGGGTTCGATTC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	57.8	57.9	0.0	0.0	2.353886	9708	9898	264-265	1.000000	68.7
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CTACTGATGCGGGTTCGATT	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	57.8	57.9	0.0	0.0	2.360001	9708	9898	265-266	1.000000	68.7
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CTTCCAAGCTACTGATGCGG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.7	56.9	0.0	0.0	2.757680	9708	9898	267-268	1.000000	65.4
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	TCAATTGGTTAGAGCACCGC	CCTACGCTGGTTCGAATCC	20	19	50.0	57.9	58.6	58.0	5.4	5.8	1.577596	27421	27525	198-198	1.000000	70.6
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	TCAATTGGTTAGAGCACCGC	ATGGGGACGGACTGTAAATC	20	20	50.0	50.0	58.6	57.0	5.4	0.0	1.553472	27421	27525	229-229	1.000000	70.4
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	TCAATTGGTTAGAGCACCGC	TGGGGACGGACTGTAAATCC	20	20	50.0	55.0	58.6	59.1	5.4	0.0	1.644240	27421	27525	228-228	1.000000	69.7
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	TCAATTGGTTAGAGCACCGC	CTGGTTCGAATCCAGCTCG	20	19	50.0	57.9	58.6	58.3	5.4	24.2	1.880380	27421	27525	192-192	1.000000	68.3
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	TCAATTGGTTAGAGCACCGC	AATGGGGACGGACTGTAAATC	20	21	50.0	47.6	58.6	57.7	5.4	0.0	1.828157	27421	27525	230-230	1.000000	68.2
petN-trnC-GCA_p1	petN-trnC-GCA	1	ACGTCACAACCCACTTCTTC	CCTCAATTCCCCAGTTCGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.080373	29219	29685	574-575	1.000000	84.2
petN-trnC-GCA_p2	petN-trnC-GCA	2	ACGTCACAACCCACTTCTTC	TTGGGACGAGGCGATATAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.059414	29219	29685	621-622	1.000000	84.0
petN-trnC-GCA_p3	petN-trnC-GCA	3	CAACCCACTTCTTCCCCAAA	CCTCAATTCCCCAGTTCGAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.110492	29219	29685	568-569	1.000000	84.0
petN-trnC-GCA_p4	petN-trnC-GCA	4	CAACCCACTTCTTCCCCAAA	TTGGGACGAGGCGATATAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.089533	29219	29685	615-616	1.000000	83.8
petN-trnC-GCA_p5	petN-trnC-GCA	5	AACCCACTTCTTCCCCAAAC	TTGGGACGAGGCGATATAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091019	29219	29685	614-615	1.000000	83.8
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	TCTTCTAAGCCAGTCGTCCA	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.184297	33788	35593	1953-1979	1.000000	86.3
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TCTTCTAAGCCAGTCGTCCA	AAGCCCTTTTAACTCAGCGG	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.546031	33788	35593	1955-1981	1.000000	83.4
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CTCTTCTAAGCCAGTCGTCC	GCCCTTTTAACTCAGCGGTA	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.673979	33788	35593	1954-1980	1.000000	82.3
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	TCTTCTAAGCCAGTCGTCCA	CGGTGATAGCTAAGCCCTTT	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.702823	33788	35593	1966-1992	1.000000	82.1
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	TCTTCTAAGCCAGTCGTCCA	GCCATGGTAAGGCGTAAGTC	20	20	50.0	55.0	58.1	58.7	0.0	14.7	0.784032	33788	35593	1926-1952	1.000000	81.6
rps4-trnL-UAA_p1	rps4-trnL-UAA	1	GCAAGGCCTGGCAAATTTTT	ACGTCTCCATACCTAGGCTC	20	20	45.0	55.0	58.4	58.0	28.2	9.3	0.407880	44661	45396	812-831	1.000000	82.3
rps4-trnL-UAA_p2	rps4-trnL-UAA	2	GCAAGGCCTGGCAAATTTTT	CGTCTCCATACCTAGGCTCA	20	20	45.0	55.0	58.4	58.0	28.2	9.3	0.409285	44661	45396	811-830	1.000000	82.3
rps4-trnL-UAA_p3	rps4-trnL-UAA	3	GCAAGGCCTGGCAAATTTTT	TCTGCCATTTCGCCATATCC	20	20	45.0	50.0	58.4	58.1	28.2	0.0	0.495726	44661	45396	771-790	1.000000	81.9
rps4-trnL-UAA_p4	rps4-trnL-UAA	4	GGCCTGGCAAATTTTTCAGA	ACGTCTCCATACCTAGGCTC	20	20	45.0	55.0	57.2	58.0	22.0	9.3	0.853466	44661	45396	808-827	1.000000	78.8
rps4-trnL-UAA_p5	rps4-trnL-UAA	5	GGCCTGGCAAATTTTTCAGA	CGTCTCCATACCTAGGCTCA	20	20	45.0	55.0	57.2	58.0	22.0	9.3	0.854872	44661	45396	807-826	1.000000	78.7
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	CCCAAAACAAACACGCTACC	ATGTTGCATCGGAATCCTCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.155495	63176	63304	220-228	1.000000	83.8
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCAAAACAAACACGCTACC	GTTGCATCGGAATCCTCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.172490	63176	63304	218-226	1.000000	83.7
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CCAAAACAAACACGCTACCG	ATGTTGCATCGGAATCCTCC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.277975	63176	63304	219-227	1.000000	82.8
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCAAAACAAACACGCTACC	GCATCGGAATCCTCCTTCTC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.290687	63176	63304	215-223	1.000000	82.8
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CCAAAACAAACACGCTACCG	GTTGCATCGGAATCCTCCTT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.294970	63176	63304	217-225	1.000000	82.7
psaJ-rpl33_p1	psaJ-rpl33	1	ATATCTCTCTACGGCCCCTG	ACCATCAGTCTCTCTCCGAG	20	20	55.0	55.0	57.8	57.9	0.0	0.0	0.275138	63485	63717	420-424	1.000000	80.6
psaJ-rpl33_p2	psaJ-rpl33	2	ATATCTCTCTACGGCCCCTG	CCATCAGTCTCTCTCCGAGT	20	20	55.0	55.0	57.8	57.9	0.0	0.0	0.275138	63485	63717	419-423	1.000000	80.6
psaJ-rpl33_p3	psaJ-rpl33	3	ATATGGTTCGGCTTACTGGC	ACCATCAGTCTCTCTCCGAG	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.307452	63485	63717	389-393	1.000000	80.6
psaJ-rpl33_p4	psaJ-rpl33	4	ATATGGTTCGGCTTACTGGC	CCATCAGTCTCTCTCCGAGT	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.307452	63485	63717	388-392	1.000000	80.6
psaJ-rpl33_p5	psaJ-rpl33	5	GCTTACTGGCTGGTTCACTC	CCATCAGTCTCTCTCCGAGT	20	20	55.0	55.0	58.6	57.9	0.0	0.0	0.607533	63485	63717	378-382	1.000000	78.3
rpl14-rpl16_p1	rpl14-rpl16	1	CCCGCTATTGTCTGCTACAT	CACCCGACTAGTGATTTCCG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.610696	77408	77873	542-546	1.000000	81.0
rpl14-rpl16_p2	rpl14-rpl16	2	CCCGCTATTGTCTGCTACAT	ACCCGACTAGTGATTTCCGA	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.689898	77408	77873	541-545	1.000000	80.4
rpl14-rpl16_p3	rpl14-rpl16	3	CCCGCTATTGTCTGCTACAT	TAGCCACCCGACTAGTGATT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.687833	77408	77873	546-550	1.000000	80.3
rpl14-rpl16_p4	rpl14-rpl16	4	CCCGCTATTGTCTGCTACAT	CAAGATGCCTATAGCCACCC	20	20	50.0	55.0	57.5	57.8	0.0	0.0	0.724394	77408	77873	557-561	1.000000	79.9
rpl14-rpl16_p5	rpl14-rpl16	5	CCCGCTATTGTCTGCTACAT	CCTATAGCCACCCGACTAGT	20	20	50.0	55.0	57.5	57.7	0.0	0.0	0.822858	77408	77873	550-554	1.000000	79.2
trnT-UGU_p1	trnT-UGU	1	TCATCACTTGATAGGAAAATGA	AATGCAAGACGACTTCTGCT	22	20	31.8	45.0	52.9	57.8	0.0	11.7	7.293768	84126	86152	2073-2073	0.500000	20.0
trnT-UGU_p2	trnT-UGU	2	TCATCACTTGATAGGAAAATGA	GAAAAGAAAGGGGTCGGGAG	22	20	31.8	55.0	52.9	58.2	0.0	0.0	7.295384	84126	86152	2105-2105	0.500000	20.0
trnT-UGU_p3	trnT-UGU	3	TCATCACTTGATAGGAAAATGA	ATGCAAGACGACTTCTGCTC	22	20	31.8	50.0	52.9	58.3	0.0	11.7	7.398291	84126	86152	2072-2072	0.500000	20.0
trnT-UGU_p4	trnT-UGU	4	TCATCACTTGATAGGAAAATGA	TCGGGAGACTTCTTCCAGAA	22	20	31.8	50.0	52.9	57.7	0.0	4.4	7.422112	84126	86152	2092-2092	0.500000	20.0
trnT-UGU_p5	trnT-UGU	5	TTCATCACTTGATAGGAAAATGA	AATGCAAGACGACTTCTGCT	23	20	30.4	45.0	53.7	57.8	0.0	11.7	7.441492	84126	86152	2074-2074	0.500000	20.0
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	CGGATCTTACACAGCTGCTT	CTCTGGCACTGCTTCCTAAG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.037091	110761	111284	1422-1428	1.000000	86.1
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	CGGATCTTACACAGCTGCTT	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.037091	110761	111284	1420-1426	1.000000	86.1
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	CGGATCTTACACAGCTGCTT	GTGGGTTAGGGAAGAGAGGT	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.061701	110761	111284	1270-1276	1.000000	86.1
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	CGGATCTTACACAGCTGCTT	TGTGGGTTAGGGAAGAGAGG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.063114	110761	111284	1271-1277	1.000000	86.1
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	CGGATCTTACACAGCTGCTT	CAGCGTGTCTACCCATTTCA	20	20	50.0	50.0	58.0	57.9	15.3	0.0	0.109042	110761	111284	1400-1406	1.000000	85.6
trnP-GGG-trnL-UAG_p1	trnP-GGG-trnL-UAG	1	GCTACCAAACTGCGCCATAT	CTCTGGCACTGCTTCCTAAG	20	20	50.0	55.0	58.7	58.0	4.2	0.0	0.712357	111359	111627	345-345	1.000000	82.2
trnP-GGG-trnL-UAG_p2	trnP-GGG-trnL-UAG	2	GCTACCAAACTGCGCCATAT	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.7	58.0	4.2	0.0	0.712357	111359	111627	343-343	1.000000	82.2
trnP-GGG-trnL-UAG_p3	trnP-GGG-trnL-UAG	3	GCTACCAAACTGCGCCATAT	CAGCGTGTCTACCCATTTCA	20	20	50.0	50.0	58.7	57.9	4.2	0.0	0.784308	111359	111627	323-323	1.000000	82.0
trnP-GGG-trnL-UAG_p4	trnP-GGG-trnL-UAG	4	GCTACCAAACTGCGCCATAT	AGCGTGTCTACCCATTTCAC	20	20	50.0	50.0	58.7	57.9	4.2	0.0	0.785764	111359	111627	322-322	1.000000	82.0
trnP-GGG-trnL-UAG_p5	trnP-GGG-trnL-UAG	5	GACCTCCATCACCCCAAGAT	CTGGCACTGCTTCCTAAGAG	20	20	55.0	55.0	58.8	58.0	0.0	0.0	0.804365	111359	111627	368-368	1.000000	81.0
trnT-UGU_p1	trnT-UGU	1	AATGCAAGACGACTTCTGCT	TCATCACTTGATAGGAAAATGA	20	22	45.0	31.8	57.8	52.9	11.7	0.0	7.293768	150227	152253	2073-2073	0.500000	20.0
trnT-UGU_p2	trnT-UGU	2	GAAAAGAAAGGGGTCGGGAG	TCATCACTTGATAGGAAAATGA	20	22	55.0	31.8	58.2	52.9	0.0	0.0	7.295384	150227	152253	2105-2105	0.500000	20.0
trnT-UGU_p3	trnT-UGU	3	ATGCAAGACGACTTCTGCTC	TCATCACTTGATAGGAAAATGA	20	22	50.0	31.8	58.3	52.9	11.7	0.0	7.398291	150227	152253	2072-2072	0.500000	20.0
trnT-UGU_p4	trnT-UGU	4	TCGGGAGACTTCTTCCAGAA	TCATCACTTGATAGGAAAATGA	20	22	50.0	31.8	57.7	52.9	4.4	0.0	7.422112	150227	152253	2092-2092	0.500000	20.0
trnT-UGU_p5	trnT-UGU	5	AATGCAAGACGACTTCTGCT	TTCATCACTTGATAGGAAAATGA	20	23	45.0	30.4	57.8	53.7	11.7	0.0	7.441492	150227	152253	2074-2074	0.500000	20.0