primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH(GUG)-psbA_p1	trnH(GUG)-psbA	1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	77	424	467-548	0.800000	72.2
trnH(GUG)-psbA_p2	trnH(GUG)-psbA	2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.307703	77	424	469-550	0.800000	72.2
trnH(GUG)-psbA_p3	trnH(GUG)-psbA	3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306133	77	424	481-562	0.800000	72.1
trnH(GUG)-psbA_p4	trnH(GUG)-psbA	4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	77	424	480-561	0.800000	72.1
trnH(GUG)-psbA_p5	trnH(GUG)-psbA	5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	77	424	482-563	0.800000	72.1
trnK(UUU)-rps16_p1	trnK(UUU)-rps16	1	AAAGCCGAGTACTCTACCGT	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.2	56.5	20.3	1.9	1.635593	4306	5076	840-995	1.000000	68.4
trnK(UUU)-rps16_p2	trnK(UUU)-rps16	2	AAGCCGAGTACTCTACCGTT	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.2	56.5	20.3	1.9	1.635593	4306	5076	839-994	1.000000	68.4
trnK(UUU)-rps16_p3	trnK(UUU)-rps16	3	TCTAGCCGCACTTAAAAGCC	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.3	56.5	0.0	1.9	1.733010	4306	5076	854-1009	1.000000	67.6
trnK(UUU)-rps16_p4	trnK(UUU)-rps16	4	CCGCACTTAAAAGCCGAGTA	AGGAACTTCCGCCTAATCAA	20	20	50.0	45.0	58.3	56.5	5.3	1.9	1.749979	4306	5076	849-1004	1.000000	67.5
trnK(UUU)-rps16_p5	trnK(UUU)-rps16	5	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	58.2	58.7	20.3	0.0	1.837155	4306	5076	893-1048	1.000000	66.7
rps16-trnQ(UUG)_p1	rps16-trnQ(UUG)	1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	6218	7375	1113-1320	1.000000	87.4
rps16-trnQ(UUG)_p2	rps16-trnQ(UUG)	2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6218	7375	1055-1262	1.000000	87.1
rps16-trnQ(UUG)_p3	rps16-trnQ(UUG)	3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6218	7375	1096-1303	1.000000	87.0
rps16-trnQ(UUG)_p4	rps16-trnQ(UUG)	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6218	7375	1038-1245	1.000000	86.7
rps16-trnQ(UUG)_p5	rps16-trnQ(UUG)	5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	58.2	58.0	7.8	0.0	0.217997	6218	7375	1114-1321	1.000000	86.4
rpoC2_p1	rpoC2	1	CCGAATCAAATGACTGCACC	GGGTCGAATCGATTGGTTGA	20	20	50.0	50.0	57.5	58.0	0.0	17.0	0.568175	16879	16998	323-323	1.000000	75.9
rpoC2_p2	rpoC2	2	CCGAATCAAATGACTGCACC	AGGCCTGAAAGAGAACGTTG	20	20	50.0	50.0	57.5	58.1	0.0	3.1	0.665398	16879	16998	302-302	1.000000	75.2
rpoC2_p3	rpoC2	3	CCGAATCAAATGACTGCACC	AGAGAACGTTGTTTTGGGGG	20	20	50.0	50.0	57.5	58.3	0.0	5.0	0.862070	16879	16998	293-293	1.000000	73.6
rpoC2_p4	rpoC2	4	CCGAATCAAATGACTGCACC	GGCCTGAAAGAGAACGTTGT	20	20	50.0	50.0	57.5	58.4	0.0	5.0	0.955303	16879	16998	301-301	1.000000	72.9
rpoC2_p5	rpoC2	5	CCGAATCAAATGACTGCACC	CGGATTGAAAGGATTCGTGC	20	20	50.0	50.0	57.5	57.5	0.0	0.0	1.008225	16879	16998	254-254	1.000000	72.6
psbC_p1	psbC	1	GTTTCTCAGGAAATTCGCGC	GCATTGGATCTCCTGTCTCA	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.112773	34118	35539	1556-1556	1.000000	71.3
psbC_p2	psbC	2	CGTTTCTCAGGAAATTCGCG	GCATTGGATCTCCTGTCTCA	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.141302	34118	35539	1557-1557	1.000000	71.0
psbC_p3	psbC	3	AGGTATTCGTGCTTGGATGG	GCATTGGATCTCCTGTCTCA	20	20	50.0	50.0	57.7	57.0	0.0	0.0	1.328312	34118	35539	1482-1482	1.000000	69.5
psbC_p4	psbC	4	GTTTCTCAGGAAATTCGCGC	CAAGCATTGGATCTCCTGTCT	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.395192	34118	35539	1559-1559	1.000000	69.0
psbC_p5	psbC	5	GTTTCTCAGGAAATTCGCGC	AGCATTGGATCTCCTGTCTC	20	20	50.0	50.0	58.1	56.7	0.0	0.0	1.414244	34118	35539	1557-1557	1.000000	68.8
psaA-ycf3_p1	psaA-ycf3	1	CTTCTGGTTCCGGCGAAC	TTGAAGATCACGAGGCGTTT	18	20	61.1	45.0	58.4	57.8	9.3	0.0	2.601183	41956	42683	776-783	1.000000	62.2
psaA-ycf3_p2	psaA-ycf3	2	CTTCTGGTTCCGGCGAAC	TTGGTTGAAGATCACGAGGC	18	20	61.1	50.0	58.4	58.2	9.3	0.0	2.628955	41956	42683	780-787	1.000000	61.9
psaA-ycf3_p3	psaA-ycf3	3	CTTCTGGTTCCGGCGAAC	TGAAGATCACGAGGCGTTTC	18	20	61.1	50.0	58.4	58.3	9.3	0.0	2.727352	41956	42683	775-782	1.000000	61.2
psaA-ycf3_p4	psaA-ycf3	4	CTTCTGGTTCCGGCGAAC	TGGTTTGATCAAGCTGCTGA	18	20	61.1	45.0	58.4	57.7	9.3	19.9	2.712499	41956	42683	854-861	1.000000	61.0
psaA-ycf3_p5	psaA-ycf3	5	CTTCTGGTTCCGGCGAAC	TCACGAGGCGTTTCGAATAA	18	20	61.1	45.0	58.4	57.6	9.3	15.7	2.788264	41956	42683	769-776	1.000000	60.7
trnT(UGU)-trnL(UAA)_p1	trnT(UGU)-trnL(UAA)	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46404	47096	730-754	1.000000	80.8
trnT(UGU)-trnL(UAA)_p2	trnT(UGU)-trnL(UAA)	2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	46404	47096	781-805	1.000000	79.8
trnT(UGU)-trnL(UAA)_p3	trnT(UGU)-trnL(UAA)	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46404	47096	762-786	1.000000	79.8
trnT(UGU)-trnL(UAA)_p4	trnT(UGU)-trnL(UAA)	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46404	47096	724-748	1.000000	79.6
trnT(UGU)-trnL(UAA)_p5	trnT(UGU)-trnL(UAA)	5	TAGGAAAAAGCCGGCTATCG	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	33.1	0.0	0.645085	46404	47096	775-799	1.000000	78.5
trnF(GAA)-ndhJ_p1	trnF(GAA)-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	48043	48706	806-806	1.000000	79.8
trnF(GAA)-ndhJ_p2	trnF(GAA)-ndhJ	2	CTACATTGGGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.119819	48043	48706	823-823	1.000000	79.5
trnF(GAA)-ndhJ_p3	trnF(GAA)-ndhJ	3	TGCTACATTGGGAATGGTCG	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.124095	48043	48706	825-825	1.000000	79.5
trnF(GAA)-ndhJ_p4	trnF(GAA)-ndhJ	4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	48043	48706	770-770	1.000000	79.4
trnF(GAA)-ndhJ_p5	trnF(GAA)-ndhJ	5	GGGATAGCTCAGCTGGTAGA	GATAATCATCCGCGCCTGAA	20	20	55.0	50.0	58.0	58.2	10.3	12.0	0.206738	48043	48706	845-845	1.000000	78.8
ndhC-trnV(UAC)_p1	ndhC-trnV(UAC)	1	CTAGAAAGTTTCTCCGGCCC	CGATCTAGACCAGAGGCAGA	20	20	55.0	55.0	58.0	58.0	21.2	0.0	0.051324	50378	51321	1367-1376	1.000000	83.3
ndhC-trnV(UAC)_p2	ndhC-trnV(UAC)	2	GGGGCTAAAATCCCGGAAAT	CGATCTAGACCAGAGGCAGA	20	20	50.0	55.0	57.9	58.0	0.7	0.0	0.107875	50378	51321	1337-1346	1.000000	82.9
ndhC-trnV(UAC)_p3	ndhC-trnV(UAC)	3	CTAGAAAGTTTCTCCGGCCC	ATCTAGACCAGAGGCAGACC	20	20	55.0	55.0	58.0	57.9	21.2	0.0	0.106242	50378	51321	1365-1374	1.000000	82.8
ndhC-trnV(UAC)_p4	ndhC-trnV(UAC)	4	AGGGGGCCATTTTCATTGTT	CGATCTAGACCAGAGGCAGA	20	20	45.0	55.0	58.0	58.0	4.4	0.0	0.069042	50378	51321	1917-1926	1.000000	82.1
ndhC-trnV(UAC)_p5	ndhC-trnV(UAC)	5	CTAAGGAGGGGGCCATTTTC	CGATCTAGACCAGAGGCAGA	20	20	55.0	55.0	57.9	58.0	4.4	0.0	0.098987	50378	51321	1923-1932	1.000000	81.8
atpB-rbcL_p1	atpB-rbcL	1	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.354276	54361	55163	932-932	1.000000	77.9
atpB-rbcL_p2	atpB-rbcL	2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	54361	55163	899-899	1.000000	77.6
atpB-rbcL_p3	atpB-rbcL	3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	54361	55163	970-970	1.000000	77.0
atpB-rbcL_p4	atpB-rbcL	4	ATTTGGATGATACGCCCCTG	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	57.7	58.3	0.0	0.0	0.578226	54361	55163	937-937	1.000000	76.1
atpB-rbcL_p5	atpB-rbcL	5	GATGATACGCCCCTGGTTTT	TAACACCCGCTTTGAATCCA	20	20	50.0	45.0	57.9	57.4	0.0	0.0	0.623197	54361	55163	933-933	1.000000	75.7
rbcL-accD_p1	rbcL-accD	1	CCGCTTGTGAGGTATGGAAA	ACCTGCGTTCTAACTCCTTC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.853465	56610	57298	821-822	1.000000	73.6
rbcL-accD_p2	rbcL-accD	2	CCGCTTGTGAGGTATGGAAA	TCCCATACCTGCGTTCTAAC	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.081644	56610	57298	827-828	1.000000	71.7
rbcL-accD_p3	rbcL-accD	3	CCGCTTGTGAGGTATGGAAA	CCCATACCTGCGTTCTAACT	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.089809	56610	57298	826-827	1.000000	71.7
rbcL-accD_p4	rbcL-accD	4	CCGCTTGTGAGGTATGGAAA	CCTGCGTTCTAACTCCTTCT	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.148446	56610	57298	820-821	1.000000	71.2
rbcL-accD_p5	rbcL-accD	5	GCTTGCAAATGGAGTCCTGA	ACCTGCGTTCTAACTCCTTC	20	20	50.0	50.0	58.5	57.3	11.3	0.0	1.207467	56610	57298	849-850	1.000000	70.7
accD-psaI_p1	accD-psaI	1	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.121766	58766	59451	802-803	1.000000	79.5
accD-psaI_p2	accD-psaI	2	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	0.0	26.8	0.198299	58766	59451	808-809	1.000000	78.9
accD-psaI_p3	accD-psaI	3	CACGCCTTCTTTCCCTTGAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.4	58.0	0.0	26.9	0.422400	58766	59451	795-796	1.000000	77.1
accD-psaI_p4	accD-psaI	4	CACGCCTTCTTTCCCTTGAA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.4	57.9	0.0	26.8	0.498933	58766	59451	801-802	1.000000	76.5
accD-psaI_p5	accD-psaI	5	TAAGCTCCACGCCTTCTTTC	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	57.9	57.5	0.0	8.9	0.547057	58766	59451	791-792	1.000000	76.1
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62975	64025	1107-1108	1.000000	75.7
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62975	64025	1109-1110	1.000000	75.7
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62975	64025	1106-1107	1.000000	75.2
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62975	64025	1108-1109	1.000000	75.2
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	62975	64025	1106-1107	1.000000	74.8
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	71207	71643	525-539	1.000000	87.7
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	GGCCGGGATCATTCAATACA	20	20	50.0	50.0	58.0	57.7	0.0	2.2	0.271304	71207	71643	557-571	1.000000	86.9
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.470628	71207	71643	528-542	1.000000	85.8
clpP-psbB_p4	clpP-psbB	4	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.3	58.2	23.8	0.0	0.494531	71207	71643	516-530	1.000000	85.8
clpP-psbB_p5	clpP-psbB	5	CGTCAACCCAAGATGCATCT	GGCCGGGATCATTCAATACA	20	20	50.0	50.0	58.3	57.7	23.8	2.2	0.527591	71207	71643	548-562	1.000000	85.0
petB_p1	petB	1	TCACAAAGTCCTGGCTTTTGA	TCCTATTATTCCTCCTCTAAGTGA	21	24	42.9	37.5	58.0	55.6	21.6	0.0	7.416122	74108	75487	1560-1561	1.000000	40.8
petB_p2	petB	2	TCACAAAGTCCTGGCTTTTGA	TCCTCCTCTAAGTGATAAATGATTGA	21	26	42.9	34.6	58.0	57.4	21.6	0.0	7.564652	74108	75487	1551-1552	1.000000	40.8
petB_p3	petB	3	TCACAAAGTCCTGGCTTTTGA	TCCTCCTCTAAGTGATAAATGA	21	22	42.9	36.4	58.0	53.4	21.6	0.0	7.593695	74108	75487	1551-1552	1.000000	40.8
petB_p4	petB	4	AGAATCACAAAGTCCTGGCT	TCCTATTATTCCTCCTCTAAGTGA	20	24	45.0	37.5	56.7	55.6	0.0	0.0	7.666528	74108	75487	1564-1565	1.000000	40.8
petB_p5	petB	5	TCACAAAGTCCTGGCTTTTGA	TCCATACTTGTAGCAATAAAATCCT	21	25	42.9	32.0	58.0	56.3	21.6	0.0	7.686454	74108	75487	1581-1582	1.000000	40.8