primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.181932	36407	36551	267-267	1.000000	80.6
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.284854	36407	36551	247-247	1.000000	80.0
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.238284	36407	36551	304-304	1.000000	79.9
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	TTCCATGACCCCTCTTAGCT	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	57.7	58.0	0.0	0.0	0.291789	36407	36551	259-259	1.000000	79.8
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TTGTGGGACATTTGTGGCAT	GAGAGATGGCTGAGTGGTTG	20	20	45.0	55.0	58.3	58.0	0.0	0.0	0.306815	36407	36551	347-347	1.000000	79.2
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	TGACCAACCTATTGCAACTCT	20	21	50.0	42.9	58.0	57.2	15.2	0.0	1.833020	112825	115050	2621-2621	0.500000	45.3
ndhF_p2	ndhF	2	AAGAGGGATCCACCGAAGAA	TGACCAACCTATTGCAACTCT	20	21	50.0	42.9	58.0	57.2	15.2	0.0	1.835606	112825	115050	2613-2613	0.500000	45.3
ndhF_p3	ndhF	3	AGAGGGATCCACCGAAGAAA	TGACCAACCTATTGCAACTCT	20	21	50.0	42.9	58.0	57.2	15.2	0.0	1.835606	112825	115050	2612-2612	0.500000	45.3
ndhF_p4	ndhF	4	CAAGTTCCACGTTAGCCAGA	TGACCAACCTATTGCAACTCT	20	21	50.0	42.9	57.8	57.2	0.0	0.0	1.952045	112825	115050	2976-2976	0.500000	44.4
ndhF_p5	ndhF	5	CACTTGAAAGAGGCACGCTA	TGACCAACCTATTGCAACTCT	20	21	50.0	42.9	58.2	57.2	0.0	0.0	1.992460	112825	115050	2459-2459	0.500000	44.1
ndhF-rpl32_p1	ndhF-rpl32	1	ACAGATTGGCCGAAAACCAT	ACCCATTTGAGACCCAACAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.081268	115051	116077	1785-1785	0.500000	59.3
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAACTGCACCGACAAATA	ACCCATTTGAGACCCAACAC	20	20	45.0	50.0	58.1	58.0	4.2	0.0	0.113748	115051	116077	2327-2327	0.500000	59.1
ndhF-rpl32_p3	ndhF-rpl32	3	ATTTAGCAACTGCACCGACA	ACCCATTTGAGACCCAACAC	20	20	45.0	50.0	58.1	58.0	4.2	0.0	0.113748	115051	116077	2331-2331	0.500000	59.1
ndhF-rpl32_p4	ndhF-rpl32	4	TAGCAACTGCACCGACAAAT	ACCCATTTGAGACCCAACAC	20	20	45.0	50.0	58.1	58.0	4.2	0.0	0.113748	115051	116077	2328-2328	0.500000	59.1
ndhF-rpl32_p5	ndhF-rpl32	5	ACAGATTGGCCGAAAACCAT	CTTTTTGACTGTCCGGTGGA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.114851	115051	116077	1336-1336	0.500000	59.1
rpl32_p1	rpl32	1	ACAACGATAAAAAGGAGTCACC	ACCCATTTGAGACCCAACAC	22	20	40.9	50.0	56.6	58.0	0.0	0.0	3.360263	116078	116251	615-615	0.500000	33.1
rpl32_p2	rpl32	2	ACAACGATAAAAAGGAGTCACC	CATGGCGGCTTACCCTAAAT	22	20	40.9	50.0	56.6	58.0	0.0	11.4	3.374383	116078	116251	908-908	0.500000	33.0
rpl32_p3	rpl32	3	ACAACGATAAAAAGGAGTCACC	TCCTAAGAGCAGCGTGTCTA	22	20	40.9	50.0	56.6	57.9	0.0	0.0	3.479917	116078	116251	938-938	0.500000	32.2
rpl32_p4	rpl32	4	ACAACGATAAAAAGGAGTCACC	AATTTCACCATGGCGGCTTA	22	20	40.9	45.0	56.6	58.2	0.0	13.0	3.509633	116078	116251	916-916	0.500000	31.9
rpl32_p5	rpl32	5	ACAACGATAAAAAGGAGTCACC	TTCGTTCTCAGTCGGGGTAT	22	20	40.9	50.0	56.6	58.2	0.0	0.0	3.512271	116078	116251	666-666	0.500000	31.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.0	58.0	9.5	1.0	0.080892	116252	116944	1424-1424	0.500000	59.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.0	58.0	9.5	1.0	0.080892	116252	116944	1423-1423	0.500000	59.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	9.5	0.0	0.107041	116252	116944	1304-1304	0.500000	59.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.162484	116252	116944	792-792	0.500000	58.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	9.5	0.0	0.223211	116252	116944	785-785	0.500000	58.2
trnL-UAG_p1	trnL-UAG	1	GTGTTGGGTCTCAAATGGGT	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.0	58.0	0.0	1.0	0.047310	116945	117024	975-975	0.500000	59.6
trnL-UAG_p2	trnL-UAG	2	GTGTTGGGTCTCAAATGGGT	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.0	58.0	0.0	1.0	0.047310	116945	117024	974-974	0.500000	59.6
trnL-UAG_p3	trnL-UAG	3	GTGTTGGGTCTCAAATGGGT	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.073458	116945	117024	855-855	0.500000	59.4
trnL-UAG_p4	trnL-UAG	4	ATACCCCGACTGAGAACGAA	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.2	58.0	0.0	1.0	0.199318	116945	117024	924-924	0.500000	58.4
trnL-UAG_p5	trnL-UAG	5	ATACCCCGACTGAGAACGAA	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.2	58.0	0.0	1.0	0.199318	116945	117024	923-923	0.500000	58.4
ccsA_p1	ccsA	1	GTGTTGGGTCTCAAATGGGT	TCTGGACCACGAGAACTCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.007825	117156	118124	1888-1888	0.500000	59.9
ccsA_p2	ccsA	2	GTGTTGGGTCTCAAATGGGT	TTCTGGACCACGAGAACTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.007825	117156	118124	1889-1889	0.500000	59.9
ccsA_p3	ccsA	3	GTGTTGGGTCTCAAATGGGT	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020360	117156	118124	2116-2116	0.500000	59.8
ccsA_p4	ccsA	4	ATTTAGGGTAAGCCGCCATG	TCTGGACCACGAGAACTCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021945	117156	118124	1595-1595	0.500000	59.8
ccsA_p5	ccsA	5	ATTTAGGGTAAGCCGCCATG	TTCTGGACCACGAGAACTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021945	117156	118124	1596-1596	0.500000	59.8
ccsA-ndhD_p1	ccsA-ndhD	1	ATGCAGCTCTTTTATGCGGA	TCTGGACCACGAGAACTCTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.045888	118125	118390	934-934	0.500000	59.6
ccsA-ndhD_p2	ccsA-ndhD	2	ATGCAGCTCTTTTATGCGGA	TTCTGGACCACGAGAACTCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.045888	118125	118390	935-935	0.500000	59.6
ccsA-ndhD_p3	ccsA-ndhD	3	TGCAGCTCTTTTATGCGGAT	TCTGGACCACGAGAACTCTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.045888	118125	118390	933-933	0.500000	59.6
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAGCTCTTTTATGCGGAT	TTCTGGACCACGAGAACTCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.045888	118125	118390	934-934	0.500000	59.6
ccsA-ndhD_p5	ccsA-ndhD	5	TGCAGCTCTTTTATGCGGAT	TTCTCGATGGCTTCTCTTGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.058422	118125	118390	1161-1161	0.500000	59.5
ndhD_p1	ndhD	1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.155611	118391	119899	2262-2262	0.500000	58.8
ndhD_p2	ndhD	2	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.198258	118391	119899	2261-2261	0.500000	58.4
ndhD_p3	ndhD	3	AGGCATTCTTTCTGGAGCAG	CAAAAGCCCGTACTCGAGAA	20	20	50.0	50.0	57.9	57.9	0.0	21.7	0.200320	118391	119899	2125-2125	0.500000	58.4
ndhD_p4	ndhD	4	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.204524	118391	119899	2252-2252	0.500000	58.4
ndhD_p5	ndhD	5	TTCTGGAGCAGTATGGGCTA	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.204524	118391	119899	2253-2253	0.500000	58.4
psaC-ndhE_p1	psaC-ndhE	1	GGACAAGCGGATTCACATCT	AATCTCGTCGCATTTTCCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034421	120275	120834	913-913	0.500000	59.7
psaC-ndhE_p2	psaC-ndhE	2	GGACAAGCGGATTCACATCT	CGAAGCATGGTTAGAGCACT	20	20	50.0	50.0	58.0	58.0	0.0	10.7	0.045574	120275	120834	970-970	0.500000	59.6
psaC-ndhE_p3	psaC-ndhE	3	TGAAGAACCCGCGAATATGG	AATCTCGTCGCATTTTCCGA	20	20	50.0	45.0	58.1	58.0	8.2	0.0	0.056478	120275	120834	1175-1175	0.500000	59.5
psaC-ndhE_p4	psaC-ndhE	4	GGACAAGCGGATTCACATCT	AAGCCGAAGCATGGTTAGAG	20	20	50.0	50.0	58.0	58.0	0.0	17.9	0.059677	120275	120834	974-974	0.500000	59.5
psaC-ndhE_p5	psaC-ndhE	5	TGAAGAACCCGCGAATATGG	CGAAGCATGGTTAGAGCACT	20	20	50.0	50.0	58.1	58.0	8.2	10.7	0.067632	120275	120834	1232-1232	0.500000	59.5
nad6_p1	nad6	1	TCGGAAAATGCGACGAGATT	TTTTGCGGTAACTGTGTCGA	20	20	45.0	45.0	58.0	58.1	0.0	2.7	0.067305	121319	121849	1273-1273	0.500000	59.5
nad6_p2	nad6	2	AGTGCTCTAACCATGCTTCG	TTTTGCGGTAACTGTGTCGA	20	20	50.0	45.0	58.0	58.1	0.0	2.7	0.078458	121319	121849	1216-1216	0.500000	59.4
nad6_p3	nad6	3	TCGGAAAATGCGACGAGATT	ATGTGTTCGCGTATGTCCAA	20	20	45.0	45.0	58.0	57.9	0.0	5.8	0.092084	121319	121849	1382-1382	0.500000	59.3
nad6_p4	nad6	4	TCGGAAAATGCGACGAGATT	TGTGTTCGCGTATGTCCAAT	20	20	45.0	45.0	58.0	57.9	0.0	5.8	0.092084	121319	121849	1381-1381	0.500000	59.3
nad6_p5	nad6	5	CTCTAACCATGCTTCGGCTT	TTTTGCGGTAACTGTGTCGA	20	20	50.0	45.0	58.0	58.1	0.0	2.7	0.092560	121319	121849	1212-1212	0.500000	59.3
nad6-ndhI_p1	nad6-ndhI	1	GCAGAATCGATGGGATTGGT	TTTTGCGGTAACTGTGTCGA	20	20	50.0	45.0	58.0	58.1	0.0	2.7	0.089850	121850	122075	522-522	0.500000	59.3
nad6-ndhI_p2	nad6-ndhI	2	GCAGAATCGATGGGATTGGT	ATGTGTTCGCGTATGTCCAA	20	20	50.0	45.0	58.0	57.9	0.0	5.8	0.114629	121850	122075	631-631	0.500000	59.1
nad6-ndhI_p3	nad6-ndhI	3	GCAGAATCGATGGGATTGGT	TGTGTTCGCGTATGTCCAAT	20	20	50.0	45.0	58.0	57.9	0.0	5.8	0.114629	121850	122075	630-630	0.500000	59.1
nad6-ndhI_p4	nad6-ndhI	4	GCAGAATCGATGGGATTGGT	CAAACAATACGAGCCGCAAG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144930	121850	122075	798-798	0.500000	58.8
nad6-ndhI_p5	nad6-ndhI	5	GCAGAATCGATGGGATTGGT	TGTGAAGTATGTGTTCGCGT	20	20	50.0	45.0	58.0	57.9	0.0	5.8	0.170135	121850	122075	639-639	0.500000	58.6
ndhI_p1	ndhI	1	GCAGAATCGATGGGATTGGT	TTTGGAATAGCGATGGGAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059809	122076	122579	1540-1540	0.500000	59.5
ndhI_p2	ndhI	2	GCAGAATCGATGGGATTGGT	ACCAGAAGCAGAGGAGGAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063143	122076	122579	1351-1351	0.500000	59.5
ndhI_p3	ndhI	3	GCAGAATCGATGGGATTGGT	GGAGCCGTATGAAGCTTAGG	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.183471	122076	122579	1573-1573	0.500000	58.5
ndhI_p4	ndhI	4	AAGGGTCCAGGAAAGGAACT	TTTGGAATAGCGATGGGAGC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.198141	122076	122579	1384-1384	0.500000	58.4
ndhI_p5	ndhI	5	AAGGGTCCAGGAAAGGAACT	ACCAGAAGCAGAGGAGGAAT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.201475	122076	122579	1195-1195	0.500000	58.4
ndhI-ndhA_p1	ndhI-ndhA	1	TCGACACAGTTACCGCAAAA	TTTGGAATAGCGATGGGAGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.095403	122580	122699	1038-1038	0.500000	59.2
ndhI-ndhA_p2	ndhI-ndhA	2	TCGACACAGTTACCGCAAAA	ACCAGAAGCAGAGGAGGAAT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.098737	122580	122699	849-849	0.500000	59.2
ndhI-ndhA_p3	ndhI-ndhA	3	ATTGGACATACGCGAACACA	TTTGGAATAGCGATGGGAGC	20	20	45.0	50.0	57.9	58.0	15.9	0.0	0.120182	122580	122699	930-930	0.500000	59.0
ndhI-ndhA_p4	ndhI-ndhA	4	TTGGACATACGCGAACACAT	TTTGGAATAGCGATGGGAGC	20	20	45.0	50.0	57.9	58.0	15.9	0.0	0.120182	122580	122699	929-929	0.500000	59.0
ndhI-ndhA_p5	ndhI-ndhA	5	TTGGACATACGCGAACACAT	ACCAGAAGCAGAGGAGGAAT	20	20	45.0	50.0	57.9	58.0	15.9	0.0	0.123516	122580	122699	740-740	0.500000	59.0
ndhA_p1	ndhA	1	TCGACACAGTTACCGCAAAA	TTGCAAATTCTTCCCCAGCT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.076732	122700	124931	2787-2787	0.500000	59.4
ndhA_p2	ndhA	2	ATTGGACATACGCGAACACA	TTGCAAATTCTTCCCCAGCT	20	20	45.0	45.0	57.9	58.0	15.9	0.0	0.101511	122700	124931	2679-2679	0.500000	59.2
ndhA_p3	ndhA	3	TTGGACATACGCGAACACAT	TTGCAAATTCTTCCCCAGCT	20	20	45.0	45.0	57.9	58.0	15.9	0.0	0.101511	122700	124931	2678-2678	0.500000	59.2
ndhA_p4	ndhA	4	TCGACACAGTTACCGCAAAA	TAGAGGCCCCAAAAGGAGAA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.116469	122700	124931	2882-2882	0.500000	59.1
ndhA_p5	ndhA	5	CTTGCGGCTCGTATTGTTTG	TTGCAAATTCTTCCCCAGCT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.131812	122700	124931	2511-2511	0.500000	58.9
ndhH_p1	ndhH	1	CCAGCATATTCAGGCCCAAT	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.0	57.7	0.0	9.9	0.318093	124933	126114	1656-1656	0.500000	57.5
ndhH_p2	ndhH	2	TTGGTACCATCCGCTAAAGC	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.0	57.7	17.2	9.9	0.345403	124933	126114	1695-1695	0.500000	57.2
ndhH_p3	ndhH	3	TGTTCCAACGAATCACACGT	TTCTGGGAAAACGTCAACGA	20	20	45.0	45.0	58.1	57.7	0.0	9.9	0.370045	124933	126114	2024-2024	0.500000	57.0
ndhH_p4	ndhH	4	AAAGGGCCAGCATATTCAGG	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	57.9	57.7	6.2	9.9	0.390410	124933	126114	1662-1662	0.500000	56.9
ndhH_p5	ndhH	5	CCAGCATATTCAGGCCCAAT	TCTGGGAAAACGTCAACGAT	20	20	50.0	45.0	58.0	57.5	0.0	9.9	0.542131	124933	126114	1655-1655	0.500000	55.7
ycf1_p1	ycf1	1	ACATACTGAAACGACTGCTGT	CCATTTCACGACACAGCAAC	21	20	42.9	50.0	57.6	58.0	0.0	0.0	1.423325	127074	127193	342-342	0.500000	48.6
ycf1_p2	ycf1	2	ACATACTGAAACGACTGCTGT	CGAACAACAGGTCCAAAGGA	21	20	42.9	50.0	57.6	58.0	0.0	0.0	1.424975	127074	127193	848-848	0.500000	48.6
ycf1_p3	ycf1	3	ACATACTGAAACGACTGCTGT	ATCTCCCAGACGTCGTAGAG	21	20	42.9	55.0	57.6	58.0	0.0	19.5	1.430472	127074	127193	266-266	0.500000	48.6
ycf1_p4	ycf1	4	ACATACTGAAACGACTGCTGT	CTCCCAGACGTCGTAGAGAA	21	20	42.9	55.0	57.6	58.3	0.0	20.4	1.651382	127074	127193	264-264	0.500000	46.8
ycf1_p5	ycf1	5	TGACATACTGAAACGACTGCT	CCATTTCACGACACAGCAAC	21	20	42.9	50.0	57.3	58.0	0.0	0.0	1.697057	127074	127193	344-344	0.500000	46.4