primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl32-tRNA-Pro_p1	rpl32-tRNA-Pro	1	AGATTTAGCCAAGGATAGTGCT	CGCAGTTTGGTAGCGTATCA	22	20	40.9	50.0	56.9	58.4	0.0	0.0	3.420363	66285	66715	596-606	1.000000	58.6
rpl32-tRNA-Pro_p2	rpl32-tRNA-Pro	2	AGATTTAGCCAAGGATAGTGCT	GGTAGCGTATCATCTTGGGG	22	20	40.9	55.0	56.9	57.5	0.0	0.0	3.513573	66285	66715	588-598	1.000000	58.0
rpl32-tRNA-Pro_p3	rpl32-tRNA-Pro	3	AGATTTAGCCAAGGATAGTGCT	AGCGTATCATCTTGGGGTGA	22	20	40.9	50.0	56.9	58.5	0.0	0.0	3.560147	66285	66715	585-595	1.000000	57.6
rpl32-tRNA-Pro_p4	rpl32-tRNA-Pro	4	AGATTTAGCCAAGGATAGTGCT	GAGGTCGTGGGTTCAAATCC	22	20	40.9	55.0	56.9	58.5	0.0	0.0	3.606182	66285	66715	563-573	1.000000	57.5
rpl32-tRNA-Pro_p5	rpl32-tRNA-Pro	5	AGATTTAGCCAAGGATAGTGCT	GGAGGTCGTGGGTTCAAATC	22	20	40.9	55.0	56.9	58.5	0.0	0.0	3.606182	66285	66715	564-574	1.000000	57.5
tRNA-Leu-tRNA-Asn_p1	tRNA-Leu-tRNA-Asn	1	GCTTAAGCATCTCGGTTCGA	GGTTCAAATCCTACCTGGGG	20	20	50.0	55.0	58.1	57.6	22.6	0.0	0.514515	67000	67086	144-144	1.000000	79.8
tRNA-Leu-tRNA-Asn_p2	tRNA-Leu-tRNA-Asn	2	TAGACACGCTGCTCTTAGGA	GGTTCAAATCCTACCTGGGG	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.561362	67000	67086	170-170	1.000000	78.8
tRNA-Leu-tRNA-Asn_p3	tRNA-Leu-tRNA-Asn	3	GCTTAAGCATCTCGGTTCGA	GGCTGTTAACCGATTTGTCG	20	20	50.0	50.0	58.1	57.5	22.6	2.5	0.598116	67000	67086	166-166	1.000000	78.6
tRNA-Leu-tRNA-Asn_p4	tRNA-Leu-tRNA-Asn	4	GGTAGACACGCTGCTCTTAG	GGTTCAAATCCTACCTGGGG	20	20	55.0	55.0	57.8	57.6	0.0	0.0	0.633380	67000	67086	172-172	1.000000	78.2
tRNA-Leu-tRNA-Asn_p5	tRNA-Leu-tRNA-Asn	5	GTAGACACGCTGCTCTTAGG	GGTTCAAATCCTACCTGGGG	20	20	55.0	55.0	57.8	57.6	0.0	0.0	0.633380	67000	67086	171-171	1.000000	78.2
tRNA-Asn-ChlL_p1	tRNA-Asn-ChlL	1	CCCCAGGTAGGATTTGAACC	ACCACCTTTTCCATAAACTGCT	20	22	55.0	40.9	57.6	58.2	0.0	0.0	2.592445	67159	67538	471-480	1.000000	65.5
tRNA-Asn-ChlL_p2	tRNA-Asn-ChlL	2	CGACAAATCGGTTAACAGCC	ACCACCTTTTCCATAAACTGCT	20	22	50.0	40.9	57.5	58.2	8.1	0.0	2.676046	67159	67538	449-458	1.000000	65.1
tRNA-Asn-ChlL_p3	tRNA-Asn-ChlL	3	CCCCAGGTAGGATTTGAACC	ACCACCTTTTCCATAAACTGC	20	21	55.0	42.9	57.6	56.7	0.0	0.0	2.743232	67159	67538	471-480	1.000000	64.3
tRNA-Asn-ChlL_p4	tRNA-Asn-ChlL	4	CGACAAATCGGTTAACAGCC	ACCACCTTTTCCATAAACTGC	20	21	50.0	42.9	57.5	56.7	8.1	0.0	2.826833	67159	67538	449-458	1.000000	63.9
tRNA-Asn-ChlL_p5	tRNA-Asn-ChlL	5	CCCCAGGTAGGATTTGAACC	CCACCTTTTCCATAAACTGCT	20	21	55.0	42.9	57.6	56.4	0.0	0.0	3.029356	67159	67538	470-479	1.000000	62.0
ndhD-tRNA-Ile_p1	ndhD-tRNA-Ile	1	ACTGCTTGTTCCTTAGCATCT	GAACCTGCGAGTTCTCCAAT	21	20	42.9	50.0	57.3	57.9	0.0	10.5	1.810813	81596	82113	644-665	1.000000	70.5
ndhD-tRNA-Ile_p2	ndhD-tRNA-Ile	2	ACTGCTTGTTCCTTAGCATCT	ATGAGTTGGGTGCCTTAACC	21	20	42.9	50.0	57.3	57.8	0.0	5.7	1.926151	81596	82113	623-644	1.000000	69.8
ndhD-tRNA-Ile_p3	ndhD-tRNA-Ile	3	ACTGCTTGTTCCTTAGCATCTT	GAACCTGCGAGTTCTCCAAT	22	20	40.9	50.0	58.0	57.9	0.0	10.5	2.126961	81596	82113	644-665	1.000000	68.0
ndhD-tRNA-Ile_p4	ndhD-tRNA-Ile	4	TGCAATGTTTACTGCTTGTTCC	GAACCTGCGAGTTCTCCAAT	22	20	40.9	50.0	58.0	57.9	0.0	10.5	2.124436	81596	82113	654-675	1.000000	67.9
ndhD-tRNA-Ile_p5	ndhD-tRNA-Ile	5	GCTATTCCAGGTATGAGTGGT	GAACCTGCGAGTTCTCCAAT	21	20	47.6	50.0	56.9	57.9	0.0	10.5	2.226984	81596	82113	620-641	1.000000	67.4
tRNA-Ile-rpl2_p1	tRNA-Ile-rpl2	1	ATTGGAGAACTCGCAGGTTC	ATTTGTTGCGTGTACCTGGA	20	20	50.0	45.0	57.9	57.7	9.8	0.0	0.364610	82188	82524	423-423	1.000000	80.3
tRNA-Ile-rpl2_p2	tRNA-Ile-rpl2	2	ATAAGCATCCATGGCTGAGC	ATTTGTTGCGTGTACCTGGA	20	20	50.0	45.0	58.1	57.7	19.8	0.0	0.357015	82188	82524	464-464	1.000000	80.1
tRNA-Ile-rpl2_p3	tRNA-Ile-rpl2	3	GGTTAAGGCACCCAACTCAT	ATTTGTTGCGTGTACCTGGA	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.479947	82188	82524	444-444	1.000000	79.2
tRNA-Ile-rpl2_p4	tRNA-Ile-rpl2	4	ATTGGAGAACTCGCAGGTTC	TTTGTTGCGTGTACCTGGAG	20	20	50.0	50.0	57.9	58.4	9.8	0.0	0.511931	82188	82524	422-422	1.000000	79.1
tRNA-Ile-rpl2_p5	tRNA-Ile-rpl2	5	ATAAGCATCCATGGCTGAGC	TTTGTTGCGTGTACCTGGAG	20	20	50.0	50.0	58.1	58.4	19.8	0.0	0.504336	82188	82524	463-463	1.000000	78.9
rpl16-rpl14_p1	rpl16-rpl14	1	TGGAGTTTCAGAAGCAGTAGC	TCGAGCACCACTATTATCTGC	21	21	47.6	47.6	58.0	57.6	0.0	0.0	2.416703	86079	86178	217-245	1.000000	66.4
rpl16-rpl14_p2	rpl16-rpl14	2	TGGAGTTTCAGAAGCAGTAGC	CGAGCACCACTATTATCTGCT	21	21	47.6	47.6	58.0	57.6	0.0	0.0	2.422946	86079	86178	216-244	1.000000	66.4
rpl16-rpl14_p3	rpl16-rpl14	3	TGAGTGGAGTTTCAGAAGCAG	TCGAGCACCACTATTATCTGC	21	21	47.6	47.6	57.9	57.6	0.0	0.0	2.488594	86079	86178	221-249	1.000000	65.8
rpl16-rpl14_p4	rpl16-rpl14	4	GAGTGGAGTTTCAGAAGCAGT	TCGAGCACCACTATTATCTGC	21	21	47.6	47.6	57.9	57.6	0.0	0.0	2.490008	86079	86178	220-248	1.000000	65.8
rpl16-rpl14_p5	rpl16-rpl14	5	TGAGTGGAGTTTCAGAAGCAG	CGAGCACCACTATTATCTGCT	21	21	47.6	47.6	57.9	57.6	0.0	0.0	2.494837	86079	86178	220-248	1.000000	65.8
ftsH_p1	ftsH	1	ACACAAAAAGATTCGGTTGCT	AGCGAATTCCACCATTCCAA	21	20	38.1	45.0	56.9	57.8	0.0	0.0	2.289897	91536	91655	1331-1331	0.500000	41.7
ftsH_p2	ftsH	2	ACACAAAAAGATTCGGTTGC	AGCGAATTCCACCATTCCAA	20	20	40.0	45.0	55.4	57.8	0.0	0.0	2.824281	91536	91655	1331-1331	0.500000	37.4
ftsH_p3	ftsH	3	CACAAAAAGATTCGGTTGCT	AGCGAATTCCACCATTCCAA	20	20	40.0	45.0	55.1	57.8	0.0	0.0	3.121043	91536	91655	1330-1330	0.500000	35.0
ftsH_p4	ftsH	4	ACACAAAAAGATTCGGTTGCT	TAGCGAATTCCACCATTCCA	21	20	38.1	45.0	56.9	56.9	0.0	0.0	3.166619	91536	91655	1332-1332	0.500000	34.7
ftsH_p5	ftsH	5	TTCGGTTGCTATTCCAATCA	AGCGAATTCCACCATTCCAA	20	20	40.0	45.0	55.0	57.8	0.0	0.0	3.169085	91536	91655	1320-1320	0.500000	34.6
tRNA-Asp-tRNA-Tyr_p1	tRNA-Asp-tRNA-Tyr	1	GTATAACGGGATTGACGGGG	GTAATGCCTACGCTGGTTCA	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.177869	95341	95513	284-290	1.000000	84.4
tRNA-Asp-tRNA-Tyr_p2	tRNA-Asp-tRNA-Tyr	2	GTATAACGGGATTGACGGGG	TGTAATGCCTACGCTGGTTC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.177869	95341	95513	285-291	1.000000	84.4
tRNA-Asp-tRNA-Tyr_p3	tRNA-Asp-tRNA-Tyr	3	GTATAACGGGATTGACGGGG	GATGTCCGAGTGGCTAAAGG	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.218002	95341	95513	327-333	1.000000	83.6
tRNA-Asp-tRNA-Tyr_p4	tRNA-Asp-tRNA-Tyr	4	GTATAACGGGATTGACGGGG	AGGCTTAGGACACCTCCTTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.205607	95341	95513	439-445	1.000000	82.5
tRNA-Asp-tRNA-Tyr_p5	tRNA-Asp-tRNA-Tyr	5	GTATAACGGGATTGACGGGG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165298	95341	95513	752-760	1.000000	82.4
tRNA-Thr-psbD_p1	tRNA-Thr-psbD	1	AGTCATCGGTTCAAATCCGG	AACCGGACCAACCAACAAAT	20	20	50.0	45.0	58.0	58.2	0.0	9.5	0.241104	96037	96761	860-873	1.000000	80.4
tRNA-Thr-psbD_p2	tRNA-Thr-psbD	2	GTGGTAGAGTAACGCCATGG	AACCGGACCAACCAACAAAT	20	20	55.0	45.0	58.1	58.2	7.6	9.5	0.280996	96037	96761	889-902	1.000000	80.0
tRNA-Thr-psbD_p3	tRNA-Thr-psbD	3	TCAGTGGTAGAGTAACGCCA	AACCGGACCAACCAACAAAT	20	20	50.0	45.0	58.1	58.2	0.0	9.5	0.303151	96037	96761	892-905	1.000000	79.8
tRNA-Thr-psbD_p4	tRNA-Thr-psbD	4	AGTCATCGGTTCAAATCCGG	TAAACCGGACCAACCAACAA	20	20	50.0	45.0	58.0	57.6	0.0	9.5	0.456129	96037	96761	862-875	1.000000	78.7
tRNA-Thr-psbD_p5	tRNA-Thr-psbD	5	AGTCATCGGTTCAAATCCGG	AAACCGGACCAACCAACAAA	20	20	50.0	45.0	58.0	58.4	0.0	9.5	0.465943	96037	96761	861-874	1.000000	78.6
tRNA-Ser-psbZ_p1	tRNA-Ser-psbZ	1	GCCATAAACCACTCGACCAT	TCCATCTGGAGAAGCAAGTAC	20	21	50.0	47.6	58.0	57.1	0.0	0.0	1.933619	99328	99655	436-450	1.000000	69.2
tRNA-Ser-psbZ_p2	tRNA-Ser-psbZ	2	GCCATAAACCACTCGACCAT	CCATCTGGAGAAGCAAGTACT	20	21	50.0	47.6	58.0	57.1	0.0	0.0	1.941268	99328	99655	435-449	1.000000	69.2
tRNA-Ser-psbZ_p3	tRNA-Ser-psbZ	3	ATGGGAGAGAGAGGGATTCG	TCCATCTGGAGAAGCAAGTAC	20	21	55.0	47.6	58.0	57.1	0.0	0.0	1.903475	99328	99655	496-510	1.000000	68.9
tRNA-Ser-psbZ_p4	tRNA-Ser-psbZ	4	ATGGGAGAGAGAGGGATTCG	CCATCTGGAGAAGCAAGTACT	20	21	55.0	47.6	58.0	57.1	0.0	0.0	1.911124	99328	99655	495-509	1.000000	68.9
tRNA-Ser-psbZ_p5	tRNA-Ser-psbZ	5	GGGAGAGAGAGGGATTCGAA	TCCATCTGGAGAAGCAAGTAC	20	21	55.0	47.6	57.9	57.1	0.0	0.0	1.969961	99328	99655	494-508	1.000000	68.4
psbZ-tRNA-Gly_p1	psbZ-tRNA-Gly	1	TCGCTTTGGATTGCTCTTGT	CGAACTCGCATCTTCTCCTT	20	20	45.0	50.0	58.1	57.7	0.0	0.0	0.389607	99845	100026	294-296	1.000000	79.3
psbZ-tRNA-Gly_p2	psbZ-tRNA-Gly	2	TCGCTTTGGATTGCTCTTGT	AACTCGCATCTTCTCCTTGG	20	20	45.0	50.0	58.1	57.6	0.0	0.0	0.497052	99845	100026	292-294	1.000000	78.5
psbZ-tRNA-Gly_p3	psbZ-tRNA-Gly	3	GCTTCTCCAGATGGATGGTC	CGAACTCGCATCTTCTCCTT	20	20	55.0	50.0	57.7	57.7	1.2	0.0	0.538803	99845	100026	345-347	1.000000	77.8
psbZ-tRNA-Gly_p4	psbZ-tRNA-Gly	4	TCGCTTTGGATTGCTCTTGT	ATCGAACTCGCATCTTCTCC	20	20	45.0	50.0	58.1	57.5	0.0	0.0	0.604289	99845	100026	296-298	1.000000	77.6
psbZ-tRNA-Gly_p5	psbZ-tRNA-Gly	5	TCGCTTTGGATTGCTCTTGT	TCGAACTCGCATCTTCTCCT	20	20	45.0	50.0	58.1	58.5	0.0	0.0	0.639047	99845	100026	295-297	1.000000	77.3
tRNA-Gly-tufA_p1	tRNA-Gly-tufA	1	GAGTTCGATTCTCGCTACCC	TCGCGAAGGAGGATTTACAA	20	20	55.0	45.0	57.9	57.0	0.0	6.2	1.165136	100098	100156	137-137	1.000000	73.0
tRNA-Gly-tufA_p2	tRNA-Gly-tufA	2	GAGTTCGATTCTCGCTACCC	TTCGCGAAGGAGGATTTACA	20	20	55.0	45.0	57.9	57.0	0.0	19.4	1.165136	100098	100156	138-138	1.000000	73.0
tRNA-Gly-tufA_p3	tRNA-Gly-tufA	3	GAGTTCGATTCTCGCTACCC	TTGTTATTCGCGAAGGAGGA	20	20	55.0	45.0	57.9	57.0	0.0	27.2	1.165136	100098	100156	144-144	1.000000	72.9
tRNA-Gly-tufA_p4	tRNA-Gly-tufA	4	CGAGTTCGATTCTCGCTACC	TCGCGAAGGAGGATTTACAA	20	20	55.0	45.0	58.3	57.0	21.2	6.2	1.298200	100098	100156	138-138	1.000000	71.9
tRNA-Gly-tufA_p5	tRNA-Gly-tufA	5	CGAGTTCGATTCTCGCTACC	TTCGCGAAGGAGGATTTACA	20	20	55.0	45.0	58.3	57.0	21.2	19.4	1.298200	100098	100156	139-139	1.000000	71.9
psbJ-petA_p1	psbJ-petA	1	AGGCAGAATTCCTCTTTGGC	TCCTGCTAGATTGCAAGGTT	20	20	50.0	45.0	58.2	56.8	0.6	4.9	1.335948	102569	103284	943-944	1.000000	70.8
psbJ-petA_p2	psbJ-petA	2	GGCAGAATTCCTCTTTGGCT	TCCTGCTAGATTGCAAGGTT	20	20	50.0	45.0	58.2	56.8	0.6	4.9	1.335948	102569	103284	942-943	1.000000	70.8
psbJ-petA_p3	psbJ-petA	3	AGGCAGAATTCCTCTTTGGC	GATCCTGCTAGATTGCAAGGT	20	21	50.0	47.6	58.2	57.8	0.6	4.9	1.361098	102569	103284	945-946	1.000000	70.6
psbJ-petA_p4	psbJ-petA	4	GGCAGAATTCCTCTTTGGCT	GATCCTGCTAGATTGCAAGGT	20	21	50.0	47.6	58.2	57.8	0.6	4.9	1.361098	102569	103284	944-945	1.000000	70.6
psbJ-petA_p5	psbJ-petA	5	GGCAGAATTCCTCTTTGGCT	ATCCTGCTAGATTGCAAGGT	20	20	50.0	45.0	58.2	56.6	0.6	4.9	1.569238	102569	103284	943-944	1.000000	69.0
accD-tRNA-Arg_p1	accD-tRNA-Arg	1	CAAACCAGTCAACCAAAGACA	AGGCTTGTAGCTCAGAGGAT	21	20	42.9	50.0	56.9	57.8	0.0	0.0	2.314218	108333	108774	536-539	1.000000	65.4
accD-tRNA-Arg_p2	accD-tRNA-Arg	2	CAAACCAGTCAACCAAAGACA	GTGTTGAGGCTTGTAGCTCA	21	20	42.9	50.0	56.9	57.8	0.0	0.0	2.310924	108333	108774	542-545	1.000000	65.3
accD-tRNA-Arg_p3	accD-tRNA-Arg	3	CAAACCAGTCAACCAAAGACA	TGTGTTGAGGCTTGTAGCTC	21	20	42.9	50.0	56.9	57.8	0.0	0.0	2.310924	108333	108774	543-546	1.000000	65.3
accD-tRNA-Arg_p4	accD-tRNA-Arg	4	CAAACCAGTCAACCAAAGACA	GAGGATAAAGAGCGCATGGT	21	20	42.9	50.0	56.9	57.8	0.0	11.5	2.387811	108333	108774	522-525	1.000000	64.9
accD-tRNA-Arg_p5	accD-tRNA-Arg	5	TCAAACCAGTCAACCAAAGACA	GTGTTGAGGCTTGTAGCTCA	22	20	40.9	50.0	58.3	57.8	0.0	0.0	2.478258	108333	108774	543-546	1.000000	64.0