primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnT-UGU_p1	rps16-trnT-UGU	1	CGGTGCTTTTTACCACATCG	CTAACCACGGAGCTAAGCAG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.313365	5054	6307	1275-1446	1.000000	87.1
rps16-trnT-UGU_p2	rps16-trnT-UGU	2	CGGTGCTTTTTACCACATCG	CTCTAACCACGGAGCTAAGC	20	20	50.0	55.0	57.8	57.8	0.0	0.0	0.452612	5054	6307	1277-1448	1.000000	86.0
rps16-trnT-UGU_p3	rps16-trnT-UGU	3	CGGGATCGAACATCAATTGC	CTAACCACGGAGCTAAGCAG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.461818	5054	6307	1314-1485	1.000000	85.7
rps16-trnT-UGU_p4	rps16-trnT-UGU	4	ATAAGTCGCACGGTGCTTTT	CTAACCACGGAGCTAAGCAG	20	20	45.0	55.0	58.5	58.1	28.4	0.0	0.552697	5054	6307	1285-1456	1.000000	85.1
rps16-trnT-UGU_p5	rps16-trnT-UGU	5	CGGGATCGAACATCAATTGC	CTCTAACCACGGAGCTAAGC	20	20	50.0	55.0	57.6	57.8	0.0	0.0	0.601065	5054	6307	1316-1487	1.000000	84.6
trnT-GGU-trnM-CAU_p1	trnT-GGU-trnM-CAU	1	ACGCCTTACCATGGTGTTAC	TCGGTCAACAAACTGAGCAA	20	20	50.0	45.0	57.9	58.0	28.2	4.0	0.134796	21354	21782	1143-1180	1.000000	88.0
trnT-GGU-trnM-CAU_p2	trnT-GGU-trnM-CAU	2	AACAGAAGGGAGGAGGTCAA	TCGGTCAACAAACTGAGCAA	20	20	50.0	45.0	57.9	58.0	0.0	4.0	0.149662	21354	21782	1455-1492	1.000000	86.5
trnT-GGU-trnM-CAU_p3	trnT-GGU-trnM-CAU	3	CTTGCGGAGTCCAAAGAACT	TCGGTCAACAAACTGAGCAA	20	20	50.0	45.0	58.1	58.0	10.5	4.0	0.147023	21354	21782	1490-1512	0.444444	58.9
trnT-GGU-trnM-CAU_p4	trnT-GGU-trnM-CAU	4	GCGGAGTCCAAAGAACTGAA	TCGGTCAACAAACTGAGCAA	20	20	50.0	45.0	58.1	58.0	10.5	4.0	0.152305	21354	21782	1487-1509	0.444444	58.9
trnT-GGU-trnM-CAU_p5	trnT-GGU-trnM-CAU	5	TCTTGCGGAGTCCAAAGAAC	TCGGTCAACAAACTGAGCAA	20	20	50.0	45.0	58.1	58.0	17.9	4.0	0.152305	21354	21782	1491-1513	0.444444	58.8
rpoC2-atpH_p1	rpoC2-atpH	1	TTTTTCACCCGGAGAACTGG	CTCGCGATACCTTCTACAGC	20	20	50.0	55.0	58.0	58.0	0.7	10.4	0.063667	36869	38003	1678-2852	1.000000	91.5
rpoC2-atpH_p2	rpoC2-atpH	2	GAACTGGTTGGATTGTTGCG	CTCGCGATACCTTCTACAGC	20	20	50.0	55.0	57.9	58.0	0.0	10.4	0.089804	36869	38003	1665-2839	1.000000	91.3
rpoC2-atpH_p3	rpoC2-atpH	3	TTTTTCACCCGGAGAACTGG	GATTACGAAGGGGAAGGCTG	20	20	50.0	55.0	58.0	58.0	0.7	0.0	0.069413	36869	38003	1983-2833	0.888889	87.0
rpoC2-atpH_p4	rpoC2-atpH	4	ACGCAAGGTCTTCCAAAAGT	CTCGCGATACCTTCTACAGC	20	20	45.0	55.0	57.9	58.0	0.0	10.4	0.092109	36869	38003	1977-2827	0.888889	86.8
rpoC2-atpH_p5	rpoC2-atpH	5	GAACTGGTTGGATTGTTGCG	GATTACGAAGGGGAAGGCTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.095550	36869	38003	1970-2820	0.888889	86.8
trnV-UAC-trnM-CAU_p1	trnV-UAC-trnM-CAU	1	TACCCACTGAGCTATAGCCC	GTATTGCTTTCATACGGCGG	20	20	55.0	50.0	58.0	57.3	21.2	13.2	0.674401	45810	47126	1376-2417	1.000000	81.1
trnV-UAC-trnM-CAU_p2	trnV-UAC-trnM-CAU	2	AGTAAGACGGGGAAAGGTGA	GTATTGCTTTCATACGGCGG	20	20	50.0	50.0	58.0	57.3	0.0	13.2	0.683086	45810	47126	1459-2500	1.000000	80.8
trnV-UAC-trnM-CAU_p3	trnV-UAC-trnM-CAU	3	AAGGGCCCCTTTGAAAAACA	GTATTGCTTTCATACGGCGG	20	20	45.0	50.0	58.1	57.3	21.4	13.2	0.776857	45810	47126	1422-2463	1.000000	80.1
trnV-UAC-trnM-CAU_p4	trnV-UAC-trnM-CAU	4	AGGGCCCCTTTGAAAAACAT	GTATTGCTTTCATACGGCGG	20	20	45.0	50.0	57.9	57.3	21.4	13.2	0.780148	45810	47126	1421-2462	1.000000	80.1
trnV-UAC-trnM-CAU_p5	trnV-UAC-trnM-CAU	5	TGAAGGGCCCCTTTGAAAAA	GTATTGCTTTCATACGGCGG	20	20	45.0	50.0	57.8	57.3	21.4	13.2	0.845795	45810	47126	1424-2465	1.000000	79.6
trnW-CAA-trnP-UGG_p1	trnW-CAA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	68907	69083	251-266	1.000000	86.7
trnW-CAA-trnP-UGG_p2	trnW-CAA-trnP-UGG	2	TGCCGAACTGAACTAAGAGC	ATTGAAGGGATGTAGCGCAG	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.121244	68907	69083	272-287	1.000000	86.2
trnW-CAA-trnP-UGG_p3	trnW-CAA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	ATTGAAGGGATGTAGCGCAG	20	20	50.0	50.0	58.0	58.0	0.0	11.5	0.077786	68907	69083	291-306	1.000000	86.1
trnW-CAA-trnP-UGG_p4	trnW-CAA-trnP-UGG	4	TTTTGGAGACCCACGTTCTG	ATTGAAGGGATGTAGCGCAG	20	20	50.0	50.0	58.0	58.0	0.0	11.5	0.079165	68907	69083	290-305	1.000000	86.1
trnW-CAA-trnP-UGG_p5	trnW-CAA-trnP-UGG	5	ACCTACGACATCGGGTTTTG	ATTGAAGGGATGTAGCGCAG	20	20	50.0	50.0	57.9	58.0	6.2	11.5	0.129908	68907	69083	305-320	1.000000	85.4
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTGGGAAATGCTCGGGAA	TAACACCCTTTCGCACACAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042324	69697	70144	842-932	0.777778	75.5
psaJ-rpl33_p2	psaJ-rpl33	2	TTTTGGGAAATGCTCGGGAA	TTAACACCCTTTCGCACACA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042324	69697	70144	843-933	0.666667	69.5
psaJ-rpl33_p3	psaJ-rpl33	3	TTTTTGGGAAATGCTCGGGA	TAACACCCTTTCGCACACAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042324	69697	70144	843-933	0.666667	69.5
psaJ-rpl33_p4	psaJ-rpl33	4	TTTTTGGGAAATGCTCGGGA	TTAACACCCTTTCGCACACA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.042324	69697	70144	844-934	0.666667	69.5
psaJ-rpl33_p5	psaJ-rpl33	5	TCGATTTCACCGAATCCACC	TAACACCCTTTCGCACACAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.058360	69697	70144	1178-1268	0.444444	57.8
rps2-clpP_p1	rps2-clpP	1	CCGCATCTCTTTTCGGAAGA	AAGCAACGGAGCCATCATAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051103	74350	75831	1761-2654	1.000000	91.6
rps2-clpP_p2	rps2-clpP	2	AATGACACCGAGCGCTTATT	AAGCAACGGAGCCATCATAG	20	20	45.0	50.0	58.0	58.0	13.3	0.0	0.065134	74350	75831	1629-2522	1.000000	91.5
rps2-clpP_p3	rps2-clpP	3	TTCGTTAACATACCGCTGGG	AAGCAACGGAGCCATCATAG	20	20	50.0	50.0	58.0	58.0	6.4	0.0	0.053895	74350	75831	1703-2559	0.777778	82.7
rps2-clpP_p4	rps2-clpP	4	CCGCATCTCTTTTCGGAAGA	GTATCGCCCAACCCTGTATG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.131192	74350	75831	1406-2264	0.444444	68.7
rps2-clpP_p5	rps2-clpP	5	TTCGTTAACATACCGCTGGG	GTATCGCCCAACCCTGTATG	20	20	50.0	55.0	58.0	58.1	6.4	0.0	0.133984	74350	75831	1311-2169	0.444444	68.7
clpP_p1	clpP	1	ATCTCATACGGCTCCTCGAA	TCAGAATTACCACCCAGGGT	20	20	50.0	50.0	58.0	57.9	0.0	4.8	0.071087	75832	77841	2557-2564	0.333333	53.8
clpP_p2	clpP	2	AAATCTAAGGACCCGTTCGC	TCAGAATTACCACCCAGGGT	20	20	50.0	50.0	58.0	57.9	0.0	4.8	0.073694	75832	77841	2527-2534	0.333333	53.8
clpP_p3	clpP	3	ATCTCATACGGCTCCTCGAA	CTTCATGAAGCCACTCGGAA	20	20	50.0	50.0	58.0	57.9	0.0	22.0	0.116481	75832	77841	2743-2750	0.333333	53.4
clpP_p4	clpP	4	TCTAAGGACCCGTTCGCTAT	TCAGAATTACCACCCAGGGT	20	20	50.0	50.0	57.9	57.9	0.0	4.8	0.116717	75832	77841	2524-2531	0.333333	53.4
clpP_p5	clpP	5	CGCCACAAATTCCATCCCTA	TCAGAATTACCACCCAGGGT	20	20	50.0	50.0	57.9	57.9	0.0	4.8	0.105081	75832	77841	2695-2702	0.222222	48.4
rpl36-rps8_p1	rpl36-rps8	1	TTTATCCGGACTTGTGCTCG	AGTCGACCTGGTCTACGAAT	20	20	50.0	50.0	58.0	57.9	6.1	23.5	0.130356	79779	80555	1410-2223	1.000000	90.1
rpl36-rps8_p2	rpl36-rps8	2	TTTATCCGGACTTGTGCTCG	GAAGTCGAGTTTTTGGTGCG	20	20	50.0	50.0	58.0	58.0	6.1	7.0	0.023300	79779	80555	1889-2714	1.000000	89.8
rpl36-rps8_p3	rpl36-rps8	3	GGCGATCCTTTTCGGTTACT	GAAGTCGAGTTTTTGGTGCG	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.038665	79779	80555	1817-2642	1.000000	89.8
rpl36-rps8_p4	rpl36-rps8	4	GGCGATCCTTTTCGGTTACT	AAAACGGATCAGTCGACCTG	20	20	50.0	50.0	58.0	57.9	0.0	13.0	0.100234	79779	80555	1360-2111	0.666667	76.1
rpl36-rps8_p5	rpl36-rps8	5	TTTATCCGGACTTGTGCTCG	AAAACGGATCAGTCGACCTG	20	20	50.0	50.0	58.0	57.9	6.1	13.0	0.084869	79779	80555	1432-2183	0.666667	75.8
rpl2-trnI-CAU_p1	rpl2-trnI-CAU	1	ACTGTCTACGGTTCCATTGC	CCCTAGTACGAGGATCCCTC	20	20	50.0	60.0	57.9	57.8	0.0	22.4	0.298233	86299	87388	742-1248	1.000000	87.7
rpl2-trnI-CAU_p2	rpl2-trnI-CAU	2	ACTGTCTACGGTTCCATTGC	CCCTCTTAAGCATCCATGGC	20	20	50.0	55.0	57.9	58.4	0.0	7.0	0.480926	86299	87388	727-1233	1.000000	86.3
rpl2-trnI-CAU_p3	rpl2-trnI-CAU	3	ACTGTCTACGGTTCCATTGC	CACCCTAGTACGAGGATCCC	20	20	50.0	60.0	57.9	58.4	0.0	22.4	0.476275	86299	87388	744-1250	1.000000	86.2
rpl2-trnI-CAU_p4	rpl2-trnI-CAU	4	ACTGTCTACGGTTCCATTGC	TGGCGAATTCGTAGGTTCAA	20	20	50.0	45.0	57.9	57.5	0.0	27.2	0.553823	86299	87388	679-1185	1.000000	86.0
rpl2-trnI-CAU_p5	rpl2-trnI-CAU	5	ACTGTCTACGGTTCCATTGC	TTGGCGAATTCGTAGGTTCA	20	20	50.0	45.0	57.9	57.5	0.0	27.2	0.553823	86299	87388	680-1186	1.000000	86.0
trnI-GAU-trnA-UGC_p1	trnI-GAU-trnA-UGC	1	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	20	20	50.0	50.0	58.0	57.7	0.0	4.9	0.341137	103952	104095	177-241	1.000000	86.8
trnI-GAU-trnA-UGC_p2	trnI-GAU-trnA-UGC	2	AGGTCTCTGGTTCAAGTCCA	CAACGACCCAATTGCAAGAG	20	20	50.0	50.0	57.9	57.7	0.0	4.9	0.439708	103952	104095	159-223	1.000000	86.5
trnI-GAU-trnA-UGC_p3	trnI-GAU-trnA-UGC	3	TGATTTACTTCACGGGCGAG	CCGCCTGGACAATTAGACAT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.336646	103952	104095	211-275	1.000000	86.0
trnI-GAU-trnA-UGC_p4	trnI-GAU-trnA-UGC	4	AGGTCTCTGGTTCAAGTCCA	CCGCCTGGACAATTAGACAT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.435217	103952	104095	193-257	1.000000	85.6
trnI-GAU-trnA-UGC_p5	trnI-GAU-trnA-UGC	5	TGATTTACTTCACGGGCGAG	AGACATCCAACCCGTAATCG	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.601486	103952	104095	197-261	1.000000	84.2
ndhF-rpl32_p1	ndhF-rpl32	1	GCATATTGATATGTCTGTTCCA	AGCCCTTCCCTTTCCAAATC	22	20	36.4	50.0	54.1	57.8	11.5	0.0	6.185979	125913	126885	1007-1122	1.000000	50.7
ndhF-rpl32_p2	ndhF-rpl32	2	GCATATTGATATGTCTGTTCCA	GAGCCCTTCCCTTTCCAAAT	22	20	36.4	50.0	54.1	57.8	11.5	0.0	6.185979	125913	126885	1008-1123	1.000000	50.7
ndhF-rpl32_p3	ndhF-rpl32	3	GCATATTGATATGTCTGTTCCA	GCCCTTCCCTTTCCAAATCT	22	20	36.4	50.0	54.1	57.8	11.5	0.0	6.185979	125913	126885	1006-1121	1.000000	50.7
ndhF-rpl32_p4	ndhF-rpl32	4	GCATATTGATATGTCTGTTCCAT	AGCCCTTCCCTTTCCAAATC	23	20	34.8	50.0	54.6	57.8	11.5	0.0	6.604797	125913	126885	1007-1122	1.000000	50.7
ndhF-rpl32_p5	ndhF-rpl32	5	GCATATTGATATGTCTGTTCCAT	GCCCTTCCCTTTCCAAATCT	23	20	34.8	50.0	54.6	57.8	11.5	0.0	6.604797	125913	126885	1006-1121	1.000000	50.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCTTTGGCATTGGGGAAATC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036250	127045	127447	499-872	0.777778	75.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCTTTGGCATTGGGGAAATC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037621	127045	127447	500-873	0.777778	75.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCTTTGGCATTGGGGAAATC	AGAGGATGGTATATGCCGCT	20	20	50.0	50.0	58.0	58.1	0.0	5.3	0.084243	127045	127447	563-936	0.777778	75.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCTTTGGCATTGGGGAAATC	AAAAGGCCCGAAGTAGCAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.038714	127045	127447	1026-1466	0.777778	74.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCTTTGGCATTGGGGAAATC	AATGACTAGAAGTGCGGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044156	127045	127447	1162-1602	0.777778	74.5
ycf1_p1	ycf1	1	ATACTCCCCAGAAAGCCCTT	GCATAGGTTGGGGTTTGGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.081285	140531	140650	914-1046	1.000000	91.3
ycf1_p2	ycf1	2	CCAAATCCTCAGACCACTCG	GCATAGGTTGGGGTTTGGAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.084101	140531	140650	713-845	1.000000	91.3
ycf1_p3	ycf1	3	ACCACTCGTTCTGTTGCAAT	GCATAGGTTGGGGTTTGGAA	20	20	45.0	50.0	58.0	58.1	0.6	0.0	0.101046	140531	140650	701-833	1.000000	91.2
ycf1_p4	ycf1	4	TCCCCAGAAAGCCCTTAGAA	GCATAGGTTGGGGTTTGGAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.122112	140531	140650	910-1042	1.000000	91.0
ycf1_p5	ycf1	5	ATACTCCCCAGAAAGCCCTT	TCAGCACGGGGTAATCAAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.120870	140531	140650	1133-1223	0.444444	64.1
trnA-UGC-trnI-GAU_p1	trnA-UGC-trnI-GAU	1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	4.9	0.0	0.341137	150395	150538	177-241	1.000000	86.8
trnA-UGC-trnI-GAU_p2	trnA-UGC-trnI-GAU	2	CAACGACCCAATTGCAAGAG	AGGTCTCTGGTTCAAGTCCA	20	20	50.0	50.0	57.7	57.9	4.9	0.0	0.439708	150395	150538	159-223	1.000000	86.5
trnA-UGC-trnI-GAU_p3	trnA-UGC-trnI-GAU	3	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.336646	150395	150538	211-275	1.000000	86.0
trnA-UGC-trnI-GAU_p4	trnA-UGC-trnI-GAU	4	CCGCCTGGACAATTAGACAT	AGGTCTCTGGTTCAAGTCCA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.435217	150395	150538	193-257	1.000000	85.6
trnA-UGC-trnI-GAU_p5	trnA-UGC-trnI-GAU	5	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.601486	150395	150538	197-261	1.000000	84.2
trnI-CAU-rpl2_p1	trnI-CAU-rpl2	1	CCCTAGTACGAGGATCCCTC	ACTGTCTACGGTTCCATTGC	20	20	60.0	50.0	57.8	57.9	22.4	0.0	0.298233	167102	168191	742-1248	1.000000	87.6
trnI-CAU-rpl2_p2	trnI-CAU-rpl2	2	CACCCTAGTACGAGGATCCC	ACTGTCTACGGTTCCATTGC	20	20	60.0	50.0	58.4	57.9	22.4	0.0	0.476275	167102	168191	744-1250	1.000000	86.2
trnI-CAU-rpl2_p3	trnI-CAU-rpl2	3	CCCTCTTAAGCATCCATGGC	ACTGTCTACGGTTCCATTGC	20	20	55.0	50.0	58.4	57.9	7.0	0.0	0.480926	167102	168191	727-1233	1.000000	86.2
trnI-CAU-rpl2_p4	trnI-CAU-rpl2	4	TGGCGAATTCGTAGGTTCAA	ACTGTCTACGGTTCCATTGC	20	20	45.0	50.0	57.5	57.9	27.2	0.0	0.553823	167102	168191	679-1185	1.000000	85.9
trnI-CAU-rpl2_p5	trnI-CAU-rpl2	5	TTGGCGAATTCGTAGGTTCA	ACTGTCTACGGTTCCATTGC	20	20	45.0	50.0	57.5	57.9	27.2	0.0	0.553823	167102	168191	680-1186	1.000000	85.9