primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	accD-psaI	1	TGGATGCAATTGTACCACGT	ATTGCAAGTGCCGGAAAAAC	20	20	45.0	45.0	57.8	58.1	7.4	5.0	0.328098	57941	58581	810-880	1.000000	79.0
accD-psaI_p2	accD-psaI	2	TGGATGCAATTGTACCACGT	CATTGCAAGTGCCGGAAAAA	20	20	45.0	45.0	57.8	58.1	7.4	5.0	0.329396	57941	58581	811-881	1.000000	79.0
accD-psaI_p3	accD-psaI	3	TGGATGCAATTGTACCACGT	AAAGAAGCCATTGCAAGTGC	20	20	45.0	45.0	57.8	57.8	7.4	13.5	0.351991	57941	58581	819-889	1.000000	78.8
accD-psaI_p4	accD-psaI	4	TGGATGCAATTGTACCACGT	TGCAAGTGCCGGAAAAACTA	20	20	45.0	45.0	57.8	58.3	7.4	5.0	0.511125	57941	58581	808-878	1.000000	77.5
accD-psaI_p5	accD-psaI	5	TGGATGCAATTGTACCACGT	GCAAGTGCCGGAAAAACTAG	20	20	45.0	50.0	57.8	57.7	7.4	5.0	0.524473	57941	58581	807-877	1.000000	77.4
ndhF_p1	ndhF	1	GGATGATGAATTCCACGTTCG	TGAAATCCACATGAGAGCCC	21	20	47.6	50.0	57.4	57.6	2.3	0.0	2.017524	110022	112262	80-2984	0.750000	65.9
ndhF_p2	ndhF	2	GGATGATGAATTCCACGTTCG	AAATCCACATGAGAGCCCTC	21	20	47.6	50.0	57.4	57.3	2.3	0.0	2.317602	110022	112262	80-2982	0.750000	63.5
ndhF_p3	ndhF	3	GGATGATGAATTCCACGTTCG	GAAATCCACATGAGAGCCCT	21	20	47.6	50.0	57.4	57.3	2.3	0.0	2.317602	110022	112262	80-2983	0.750000	63.5
ndhF_p4	ndhF	4	GATGAATTCCACGTTCGAACA	TGAAATCCACATGAGAGCCC	21	20	42.9	50.0	56.9	57.6	26.7	0.0	2.500196	110022	112262	80-2980	0.750000	62.0
ndhF_p5	ndhF	5	TGATGAATTCCACGTTCGAAC	TGAAATCCACATGAGAGCCC	21	20	42.9	50.0	56.9	57.6	18.5	0.0	2.500196	110022	112262	80-2981	0.750000	62.0
ndhF-rpl32_p1	ndhF-rpl32	1	TCCACATGCGTCGAAGATTT	TTTTTCCCCGTTTGGTTCCT	20	20	45.0	45.0	57.9	57.8	1.4	0.0	0.255570	112263	113113	80-1229	0.750000	80.0
ndhF-rpl32_p2	ndhF-rpl32	2	GCAATAGGTCGTGTGAACCA	TTTTTCCCCGTTTGGTTCCT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.358930	112263	113113	80-1620	0.750000	79.1
ndhF-rpl32_p3	ndhF-rpl32	3	TGCAATAGGTCGTGTGAACC	TTTTTCCCCGTTTGGTTCCT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.358930	112263	113113	80-1621	0.750000	79.1
ndhF-rpl32_p4	ndhF-rpl32	4	GGTCGTGTGAACCAAAAACC	TTTTTCCCCGTTTGGTTCCT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.360056	112263	113113	80-1614	0.750000	79.1
ndhF-rpl32_p5	ndhF-rpl32	5	TGGTTGCTGTCGGAAAAAGT	TTTTTCCCCGTTTGGTTCCT	20	20	45.0	45.0	58.2	57.8	0.0	0.0	0.404582	112263	113113	80-1208	0.750000	78.8
rpl32-trnL_p1	rpl32-trnL	1	TCGCTCGATATGGAGTACGA	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	57.8	58.0	15.2	6.7	0.173854	113288	114247	80-1793	0.750000	80.6
rpl32-trnL_p2	rpl32-trnL	2	TCGCTCGATATGGAGTACGA	TCGAACCGAGATGCTTTAGC	20	20	50.0	50.0	57.8	58.1	15.2	0.0	0.236757	113288	114247	80-1283	0.750000	80.1
rpl32-trnL_p3	rpl32-trnL	3	TCGCTCGATATGGAGTACGA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.8	57.9	15.2	0.0	0.283604	113288	114247	80-1257	0.750000	79.7
rpl32-trnL_p4	rpl32-trnL	4	TCGCTCGATATGGAGTACGA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	57.8	57.8	15.2	0.0	0.344331	113288	114247	80-1250	0.750000	79.2
rpl32-trnL_p5	rpl32-trnL	5	TCGCTCGATATGGAGTACGA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	57.8	57.8	15.2	0.0	0.355622	113288	114247	80-1255	0.750000	79.2
trnL_p1	trnL	1	AGCGAAATCAATAGGTTCTTGA	TGCAGACTGAGGCATTTTGT	22	20	36.4	45.0	55.8	58.0	0.0	6.7	4.202419	114248	114327	80-1324	0.750000	48.4
trnL_p2	trnL	2	AGCGAAATCAATAGGTTCTTGA	AAAGTAGGTCCGACTTTGCG	22	20	36.4	50.0	55.8	58.2	0.0	24.4	4.395886	114248	114327	80-1464	0.750000	46.8
trnL_p3	trnL	3	AGCGAAATCAATAGGTTCTTGA	GTAGGTCCGACTTTGCGAAA	22	20	36.4	50.0	55.8	58.2	0.0	0.0	4.400879	114248	114327	80-1461	0.750000	46.8
trnL_p4	trnL	4	AAGCGAAATCAATAGGTTCTTGA	TGCAGACTGAGGCATTTTGT	23	20	34.8	45.0	56.5	58.0	0.0	6.7	4.487744	114248	114327	80-1325	0.750000	46.1
trnL_p5	trnL	5	AGCGAAATCAATAGGTTCTTGAA	TGCAGACTGAGGCATTTTGT	23	20	34.8	45.0	56.5	58.0	0.0	6.7	4.487744	114248	114327	80-1324	0.750000	46.1
ccsA_p1	ccsA	1	GCTAAAGCATCTCGGTTCGA	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.1	58.0	0.0	8.5	0.110111	114439	115425	80-1717	0.750000	81.1
ccsA_p2	ccsA	2	GTTTTGAACAAGTTGCCGCT	TTATCGATGGCTTCCCTTGC	20	20	45.0	50.0	58.1	58.0	1.8	8.5	0.111438	114439	115425	80-1776	0.750000	81.1
ccsA_p3	ccsA	3	AAGTTGCCGCTATGGTGAAA	TTATCGATGGCTTCCCTTGC	20	20	45.0	50.0	58.1	58.0	0.0	8.5	0.128395	114439	115425	80-1767	0.750000	81.0
ccsA_p4	ccsA	4	TAGACACGCTGCTCTTAGGA	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	57.9	58.0	0.0	8.5	0.156959	114439	115425	80-1743	0.750000	80.7
ccsA_p5	ccsA	5	AGTTGCCGCTATGGTGAAAT	TTATCGATGGCTTCCCTTGC	20	20	45.0	50.0	57.9	58.0	0.0	8.5	0.161452	114439	115425	80-1766	0.750000	80.7
ndhD_p1	ndhD	1	CCATCGGCATTCTTTCAGGA	CTCAATGTGTTCGAGCATGC	20	20	50.0	50.0	58.0	57.8	0.0	10.5	0.212349	115647	117170	80-2350	0.750000	80.3
ndhD_p2	ndhD	2	CCATCGGCATTCTTTCAGGA	AAGAACCGAGGACTGTGTTG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.283179	115647	117170	80-2265	0.750000	79.7
ndhD_p3	ndhD	3	CCATCGGCATTCTTTCAGGA	CAAGAACCGAGGACTGTGTT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.283179	115647	117170	80-2266	0.750000	79.7
ndhD_p4	ndhD	4	CCATCGGCATTCTTTCAGGA	GCATGCCCTACAGACGTATT	20	20	50.0	50.0	58.0	57.8	0.0	6.4	0.283179	115647	117170	80-2336	0.750000	79.7
ndhD_p5	ndhD	5	CCATCGGCATTCTTTCAGGA	GTTATAGCAATTGCAGCGGC	20	20	50.0	50.0	58.0	58.2	0.0	30.9	0.283903	115647	117170	80-2752	0.750000	79.7
ndhD-psaC_p1	ndhD-psaC	1	TTTCTTTCCACCCCACATGG	CTCAATGTGTTCGAGCATGC	20	20	50.0	50.0	58.0	57.8	0.0	10.5	0.177906	117171	117312	80-843	0.750000	80.6
ndhD-psaC_p2	ndhD-psaC	2	ACAGACGTTTCTTTCCACCC	CTCAATGTGTTCGAGCATGC	20	20	50.0	50.0	58.0	57.8	0.0	10.5	0.206491	117171	117312	80-850	0.750000	80.3
ndhD-psaC_p3	ndhD-psaC	3	CGAACCGGCAGATATTGGAA	CTCAATGTGTTCGAGCATGC	20	20	50.0	50.0	58.1	57.8	0.0	10.5	0.221559	117171	117312	80-423	0.750000	80.2
ndhD-psaC_p4	ndhD-psaC	4	CACCCCACATGGCTAAAAGT	CTCAATGTGTTCGAGCATGC	20	20	50.0	50.0	58.1	57.8	0.0	10.5	0.246307	117171	117312	80-835	0.750000	80.0
ndhD-psaC_p5	ndhD-psaC	5	TTTCTTTCCACCCCACATGG	AAGAACCGAGGACTGTGTTG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.248736	117171	117312	80-758	0.750000	80.0
psaC-ndhE_p1	psaC-ndhE	1	CCAGATCCAAAGAACGCGTA	CGGGGTTGTTTGGACTACAA	20	20	50.0	50.0	58.0	58.0	14.5	0.0	0.034491	117559	117806	80-1383	0.750000	81.7
psaC-ndhE_p2	psaC-ndhE	2	ATGCTTCGCGTTGTTTCATG	CGGGGTTGTTTGGACTACAA	20	20	45.0	50.0	58.0	58.0	5.8	0.0	0.062147	117559	117806	80-1221	0.750000	81.5
psaC-ndhE_p3	psaC-ndhE	3	CATGCTTCGCGTTGTTTCAT	CGGGGTTGTTTGGACTACAA	20	20	45.0	50.0	58.0	58.0	5.8	0.0	0.062147	117559	117806	80-1222	0.750000	81.5
psaC-ndhE_p4	psaC-ndhE	4	CGAACCGGCAGATATTGGAA	CGGGGTTGTTTGGACTACAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081804	117559	117806	80-1457	0.750000	81.3
psaC-ndhE_p5	psaC-ndhE	5	GCATGCTCGAACACATTGAG	CGGGGTTGTTTGGACTACAA	20	20	50.0	50.0	57.8	58.0	11.4	0.0	0.199571	117559	117806	80-1054	0.750000	80.4
ndhE_p1	ndhE	1	CCAGATCCAAAGAACGCGTA	CGGGGTTGTTTGGACTACAA	20	20	50.0	50.0	58.0	58.0	14.5	0.0	0.034491	117807	118112	80-1383	0.750000	81.7
ndhE_p2	ndhE	2	ATGCTTCGCGTTGTTTCATG	CGGGGTTGTTTGGACTACAA	20	20	45.0	50.0	58.0	58.0	5.8	0.0	0.062147	117807	118112	80-1221	0.750000	81.5
ndhE_p3	ndhE	3	CATGCTTCGCGTTGTTTCAT	CGGGGTTGTTTGGACTACAA	20	20	45.0	50.0	58.0	58.0	5.8	0.0	0.062147	117807	118112	80-1222	0.750000	81.5
ndhE_p4	ndhE	4	CGAACCGGCAGATATTGGAA	CGGGGTTGTTTGGACTACAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081804	117807	118112	80-1457	0.750000	81.3
ndhE_p5	ndhE	5	CCAGATCCAAAGAACGCGTA	TGCGGCACAACTCCTTATTT	20	20	50.0	45.0	58.0	58.1	14.5	0.0	0.100296	117807	118112	80-1569	0.750000	81.2
ndhE-ndhG_p1	ndhE-ndhG	1	GCATGCTCGAACACATTGAG	CGGGGTTGTTTGGACTACAA	20	20	50.0	50.0	57.8	58.0	11.4	0.0	0.199571	118113	118380	80-1054	0.750000	80.4
ndhE-ndhG_p2	ndhE-ndhG	2	GCATGCTCGAACACATTGAG	TTTCTGGGATCCGGTCTTCT	20	20	50.0	50.0	57.8	58.0	11.4	5.5	0.217042	118113	118380	80-1377	0.750000	80.3
ndhE-ndhG_p3	ndhE-ndhG	3	GCATGCTCGAACACATTGAG	CTGGGATCCGGTCTTCTAGT	20	20	50.0	55.0	57.8	57.9	11.4	5.5	0.244392	118113	118380	80-1374	0.750000	80.0
ndhE-ndhG_p4	ndhE-ndhG	4	GCATGCTCGAACACATTGAG	TGCGGCACAACTCCTTATTT	20	20	50.0	45.0	57.8	58.1	11.4	0.0	0.265376	118113	118380	80-1240	0.750000	79.9
ndhE-ndhG_p5	ndhE-ndhG	5	GCATGCTCGAACACATTGAG	GTCATGGTACGGGGTTGTTT	20	20	50.0	50.0	57.8	58.1	11.4	0.0	0.269418	118113	118380	80-1063	0.750000	79.8
ndhG_p1	ndhG	1	GCCGCTGCAATTGCTATAAC	TCTGCCTGTTGTTGATTGGA	20	20	50.0	45.0	58.2	57.3	29.4	0.0	0.896306	118381	118911	80-1629	0.750000	74.8
ndhG_p2	ndhG	2	TGATGCGCGTTGATTTTCTG	TCTGCCTGTTGTTGATTGGA	20	20	45.0	45.0	57.8	57.3	8.4	0.0	0.900885	118381	118911	80-1694	0.750000	74.8
ndhG_p3	ndhG	3	GATGCGCGTTGATTTTCTGT	TCTGCCTGTTGTTGATTGGA	20	20	45.0	45.0	57.8	57.3	8.4	0.0	0.902400	118381	118911	80-1693	0.750000	74.8
ndhG_p4	ndhG	4	AGCCGCTGCAATTGCTATAA	TCTGCCTGTTGTTGATTGGA	20	20	45.0	45.0	58.3	57.3	29.4	0.0	0.975951	118381	118911	80-1630	0.750000	74.2
ndhG_p5	ndhG	5	GATTGATTGATGCGCGTTGA	TCTGCCTGTTGTTGATTGGA	20	20	45.0	45.0	57.5	57.3	8.4	0.0	1.112032	118381	118911	80-1701	0.750000	73.1
ndhG-ndhI_p1	ndhG-ndhI	1	TTGTAGTCCAAACAACCCCG	TCAACAAACCCTACGAGCTG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.193726	118912	119272	80-1192	0.750000	80.5
ndhG-ndhI_p2	ndhG-ndhI	2	TTGTAGTCCAAACAACCCCG	GTCAACAAACCCTACGAGCT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.195227	118912	119272	80-1193	0.750000	80.4
ndhG-ndhI_p3	ndhG-ndhI	3	AGAAGACCGGATCCCAGAAA	TCAACAAACCCTACGAGCTG	20	20	50.0	50.0	58.0	57.8	9.5	0.0	0.211197	118912	119272	80-869	0.750000	80.3
ndhG-ndhI_p4	ndhG-ndhI	4	AGAAGACCGGATCCCAGAAA	GTCAACAAACCCTACGAGCT	20	20	50.0	50.0	58.0	57.8	9.5	0.0	0.212697	118912	119272	80-870	0.750000	80.3
ndhG-ndhI_p5	ndhG-ndhI	5	ACTAGAAGACCGGATCCCAG	TCAACAAACCCTACGAGCTG	20	20	55.0	50.0	57.9	57.8	9.5	0.0	0.238547	118912	119272	80-872	0.750000	80.1
ndhI_p1	ndhI	1	TTGTAGTCCAAACAACCCCG	GAATGCGAGAGGTTACCGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035879	119273	119755	80-1724	0.750000	81.7
ndhI_p2	ndhI	2	TTGTAGTCCAAACAACCCCG	GCGAGAGGTTACCGTTTGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035879	119273	119755	80-1720	0.750000	81.7
ndhI_p3	ndhI	3	AGAAGACCGGATCCCAGAAA	GAATGCGAGAGGTTACCGTT	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.053349	119273	119755	80-1401	0.750000	81.6
ndhI_p4	ndhI	4	AGAAGACCGGATCCCAGAAA	GCGAGAGGTTACCGTTTGAT	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.053349	119273	119755	80-1397	0.750000	81.6
ndhI_p5	ndhI	5	ACTAGAAGACCGGATCCCAG	GCGAGAGGTTACCGTTTGAT	20	20	55.0	50.0	57.9	58.0	9.5	0.0	0.080699	119273	119755	80-1400	0.750000	81.4
ndhA_p1	ndhA	1	CAGCTCGTAGGGTTTGTTGA	AGTTGAAGCTCCAAAAGGGG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.164894	119842	122032	80-2532	0.750000	80.7
ndhA_p2	ndhA	2	AGCTCGTAGGGTTTGTTGAC	AGTTGAAGCTCCAAAAGGGG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.166395	119842	122032	80-2531	0.750000	80.7
ndhA_p3	ndhA	3	CAGCTCGTAGGGTTTGTTGA	GTTGAAGCTCCAAAAGGGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.168442	119842	122032	80-2531	0.750000	80.7
ndhA_p4	ndhA	4	AGCTCGTAGGGTTTGTTGAC	GTTGAAGCTCCAAAAGGGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.169942	119842	122032	80-2530	0.750000	80.6
ndhA_p5	ndhA	5	CAGCTCGTAGGGTTTGTTGA	ATGGAAAATACGACCACCGG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.196498	119842	122032	80-2466	0.750000	80.4
ndhH_p1	ndhH	1	TTTAACCGACTCGCACGTAG	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	58.0	58.0	0.0	9.9	0.037427	122034	123215	80-1955	0.750000	81.7
ndhH_p2	ndhH	2	GTTGTTGTATCCCTGCCGAT	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	58.0	58.0	0.0	9.9	0.043575	122034	123215	80-1567	0.750000	81.7
ndhH_p3	ndhH	3	ACTAATCGATTGGGCAGCAG	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	58.0	58.0	23.3	9.9	0.046147	122034	123215	80-1896	0.750000	81.6
ndhH_p4	ndhH	4	TTGAAGGATTCCCAATGGGC	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	58.1	58.0	14.9	9.9	0.140519	122034	123215	80-1608	0.750000	80.9
ndhH_p5	ndhH	5	AAGTAGTTTTGTCCCGTCCG	GAAAACGTCAACGACTGCTG	20	20	50.0	50.0	57.8	58.0	0.0	9.9	0.164516	122034	123215	80-1635	0.750000	80.7
ycf1_p1	ycf1	1	TAACGAGTCGGCCTCAAAAA	TCATCTCAGAAGGCTCGACT	20	20	45.0	50.0	57.5	57.9	18.3	0.0	0.666079	126010	126129	80-1269	0.750000	76.7
ycf1_p2	ycf1	2	AATAACGAGTCGGCCTCAAA	TCATCTCAGAAGGCTCGACT	20	20	45.0	50.0	57.2	57.9	18.3	0.0	0.891245	126010	126129	80-1271	0.750000	74.9
ycf1_p3	ycf1	3	ATAACGAGTCGGCCTCAAAA	TCATCTCAGAAGGCTCGACT	20	20	45.0	50.0	57.2	57.9	18.3	0.0	0.891245	126010	126129	80-1270	0.750000	74.9
ycf1_p4	ycf1	4	TAACGAGTCGGCCTCAAAAA	CAATTTCCCGAGTGGTCTGA	20	20	45.0	50.0	57.5	57.5	18.3	0.0	1.002476	126010	126129	80-1449	0.750000	74.0
ycf1_p5	ycf1	5	TAACGAGTCGGCCTCAAAAA	TCAATTTCCCGAGTGGTCTG	20	20	45.0	50.0	57.5	57.5	18.3	0.0	1.002476	126010	126129	80-1450	0.750000	74.0