primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 20 20 55.0 55.0 58.1 57.9 0.0 13.4 0.194818 166 527 387-428 1.000000 88.8 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 20 20 55.0 55.0 58.1 57.9 0.0 0.0 0.203648 166 527 390-431 1.000000 88.7 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 20 20 50.0 55.0 57.9 57.9 0.0 13.4 0.199180 166 527 401-442 1.000000 88.6 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 20 20 50.0 55.0 57.9 57.9 0.0 13.4 0.200688 166 527 400-441 1.000000 88.6 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 20 20 50.0 55.0 57.9 57.9 0.0 13.4 0.200688 166 527 402-443 1.000000 88.6 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CAGGATCAGTCGCGGTTTTA TATCCCAATGGGCCGTCTAT 20 20 50.0 50.0 58.0 58.0 6.1 14.9 0.043445 4417 5105 1043-1118 1.000000 88.0 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 AGGATCAGTCGCGGTTTTAC TATCCCAATGGGCCGTCTAT 20 20 50.0 50.0 58.0 58.0 6.1 14.9 0.044833 4417 5105 1042-1117 1.000000 88.0 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CAGGATCAGTCGCGGTTTTA AAAAGGGTATGTTGCTGCCA 20 20 50.0 45.0 58.0 58.0 6.1 0.0 0.012825 4417 5105 1517-1583 1.000000 87.3 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 AGGATCAGTCGCGGTTTTAC AAAAGGGTATGTTGCTGCCA 20 20 50.0 45.0 58.0 58.0 6.1 0.0 0.014213 4417 5105 1516-1582 1.000000 87.3 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 CTCATGGGAGAAGCCTTTCC AAAAGGGTATGTTGCTGCCA 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.058131 4417 5105 1801-1868 1.000000 86.4 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.9 58.1 0.0 16.9 0.189961 6287 7419 880-1261 1.000000 90.5 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 6287 7419 859-1240 1.000000 90.3 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 0.0 16.9 0.280333 6287 7419 870-1251 1.000000 89.8 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.7 58.1 0.0 16.9 0.408164 6287 7419 882-1263 1.000000 88.7 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 TCCACAACCGATCATGTCCT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.7 58.1 0.0 16.9 0.800435 6287 7419 881-1262 1.000000 85.6 psbK-psbI_p1 psbK-psbI 1 CAAGCCGCTGTAAGTTTTCG TTTGATAGTTAAGGGCGGGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.096530 8037 8203 451-451 0.200000 47.2 psbK-psbI_p2 psbK-psbI 2 GATTCGAACCTCCGGATAGC TTTGATAGTTAAGGGCGGGC 20 20 55.0 50.0 57.9 58.0 3.6 0.0 0.114416 8037 8203 1032-1032 0.200000 47.1 psbK-psbI_p3 psbK-psbI 3 AGCCGCTGTAAGTTTTCGAT TTTGATAGTTAAGGGCGGGC 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.141764 8037 8203 449-449 0.200000 46.9 psbK-psbI_p4 psbK-psbI 4 TTTCTTTGCCAAATTGCCCG TTTGATAGTTAAGGGCGGGC 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.170680 8037 8203 551-551 0.200000 46.6 psbK-psbI_p5 psbK-psbI 5 AAGCCGCTGTAAGTTTTCGA TTTGATAGTTAAGGGCGGGC 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.173304 8037 8203 450-450 0.200000 46.6 trnR-UCU-atpA_p1 trnR-UCU-atpA 1 AGCCTTCCAAGCTAACGATG GGAACGCTTCCTACTTCAGG 20 20 50.0 55.0 58.0 58.0 0.0 8.9 0.041378 10562 10785 541-622 1.000000 90.4 trnR-UCU-atpA_p2 trnR-UCU-atpA 2 TCGGAATGAAAAGCGTCCAT GGAACGCTTCCTACTTCAGG 20 20 45.0 55.0 57.9 58.0 0.0 8.9 0.119490 10562 10785 339-417 1.000000 90.3 trnR-UCU-atpA_p3 trnR-UCU-atpA 3 CCTAGCCTTCCAAGCTAACG GGAACGCTTCCTACTTCAGG 20 20 55.0 55.0 58.1 58.0 0.0 8.9 0.070215 10562 10785 544-625 1.000000 90.2 trnR-UCU-atpA_p4 trnR-UCU-atpA 4 ATCGGAATGAAAAGCGTCCA GGAACGCTTCCTACTTCAGG 20 20 45.0 55.0 57.9 58.0 0.0 8.9 0.119490 10562 10785 340-418 1.000000 90.2 trnR-UCU-atpA_p5 trnR-UCU-atpA 5 AGCCTTCCAAGCTAACGATG CGAAGCTTATCCGGGAGATG 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.101527 10562 10785 1183-1264 1.000000 85.9 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 TGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 20 20 35.0 50.0 52.0 58.5 0.0 5.4 6.474201 30636 31775 758-1274 1.000000 49.3 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 ATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 21 20 33.3 50.0 52.7 58.5 0.0 5.4 6.746812 30636 31775 759-1275 1.000000 49.3 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 TGCGAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 21 20 33.3 50.0 52.7 58.5 0.0 5.4 6.746812 30636 31775 758-1274 1.000000 49.3 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 AATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 22 20 31.8 50.0 53.6 58.5 0.0 5.4 6.858000 30636 31775 760-1276 1.000000 49.3 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 TGCGAGAATATTTACTTCCA GTTCAATTGGTCAGAGCACC 20 20 35.0 50.0 52.0 57.0 0.0 5.4 6.994354 30636 31775 759-1275 1.000000 49.3 trnY-GUA-trnE-UUC_p1 trnY-GUA-trnE-UUC 1 TATTGGATCGCTTGTGTCGG AAGTATGCCCCCATCGTCTA 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.162521 32397 32455 770-776 0.800000 75.7 trnY-GUA-trnE-UUC_p2 trnY-GUA-trnE-UUC 2 TATTGGATCGCTTGTGTCGG TGATCCCTCTTCCCCGATTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.172096 32397 32455 1243-1270 0.600000 66.7 trnY-GUA-trnE-UUC_p3 trnY-GUA-trnE-UUC 3 TATTGGATCGCTTGTGTCGG TTGATCCCTCTTCCCCGATT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.172096 32397 32455 1244-1271 0.600000 66.7 trnY-GUA-trnE-UUC_p4 trnY-GUA-trnE-UUC 4 TATTGGATCGCTTGTGTCGG ATTGATCCCTCTTCCCCGAT 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.189972 32397 32455 1245-1272 0.600000 66.5 trnY-GUA-trnE-UUC_p5 trnY-GUA-trnE-UUC 5 TATTGGATCGCTTGTGTCGG TCCCCTCCCCAATTCTTAGG 20 20 50.0 55.0 58.1 58.1 0.0 0.0 0.158172 32397 32455 1286-1286 0.200000 46.7 psbC-trnS-UGA_p1 psbC-trnS-UGA 1 GCAGCAGGGTTTGAAAAAGG GGGTTCGAATCCCTCTCTCT 20 20 50.0 55.0 58.1 58.2 0.0 17.2 0.347155 37144 37399 339-353 1.000000 86.0 psbC-trnS-UGA_p2 psbC-trnS-UGA 2 CTGCAGCAGGGTTTGAAAAA GGGTTCGAATCCCTCTCTCT 20 20 45.0 55.0 57.4 58.2 15.3 17.2 0.817399 37144 37399 341-355 1.000000 82.2 psbC-trnS-UGA_p3 psbC-trnS-UGA 3 TGCAGCAGGGTTTGAAAAAG GGGTTCGAATCCCTCTCTCT 20 20 45.0 55.0 57.4 58.2 0.0 17.2 0.817399 37144 37399 340-354 1.000000 82.2 psbC-trnS-UGA_p4 psbC-trnS-UGA 4 TCGCGATTTTGAACCTGTTC GGGTTCGAATCCCTCTCTCT 20 20 45.0 55.0 57.0 58.2 3.7 17.2 1.178450 37144 37399 313-327 1.000000 80.0 psbC-trnS-UGA_p5 psbC-trnS-UGA 5 CGCGATTTTGAACCTGTTCT GGGTTCGAATCCCTCTCTCT 20 20 45.0 55.0 57.0 58.2 0.0 17.2 1.186372 37144 37399 312-326 1.000000 80.0 psbZ-trnfM-CAU_p1 psbZ-trnfM-CAU 1 ATATGATACCGGGACAGGCA CTTGAGGTCACGGGTTCAAA 20 20 50.0 50.0 58.0 58.0 6.3 4.2 0.062382 38059 38820 1523-1736 0.800000 79.0 psbZ-trnfM-CAU_p2 psbZ-trnfM-CAU 2 ATATGATACCGGGACAGGCA CGGGACTTTGGGTTATCTGG 20 20 50.0 55.0 58.0 58.0 6.3 0.0 0.062563 38059 38820 1814-2053 0.800000 78.2 psbZ-trnfM-CAU_p3 psbZ-trnfM-CAU 3 ATATGATACCGGGACAGGCA TCTTGATTGCCTCCACATCG 20 20 50.0 50.0 58.0 58.0 6.3 0.0 0.052596 38059 38820 2202-2446 0.800000 77.4 psbZ-trnfM-CAU_p4 psbZ-trnfM-CAU 4 ATATGATACCGGGACAGGCA GGTTCTTCTATTCAGGGCGG 20 20 50.0 55.0 58.0 58.0 6.3 0.0 0.069249 38059 38820 1578-1669 0.600000 68.9 psbZ-trnfM-CAU_p5 psbZ-trnfM-CAU 5 ATATGATACCGGGACAGGCA GTTCTTCTATTCAGGGCGGG 20 20 50.0 55.0 58.0 58.0 6.3 0.0 0.069249 38059 38820 1577-1668 0.600000 68.9 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 48734 49740 1001-1124 1.000000 88.2 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 48734 49740 1009-1132 1.000000 87.6 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 48734 49740 1033-1156 1.000000 87.2 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 48734 49740 1041-1164 1.000000 86.6 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 48734 49740 1015-1138 1.000000 86.2 petA-psbJ_p1 petA-psbJ 1 GGCTTCTGTTATTTTGGCACA ATTCCGGATTGGGTTCATCC 21 20 42.9 50.0 57.3 57.6 0.0 4.1 2.023729 65863 66963 1179-1212 1.000000 75.8 petA-psbJ_p2 petA-psbJ 2 TGGCTTCTGTTATTTTGGCAC ATTCCGGATTGGGTTCATCC 21 20 42.9 50.0 57.3 57.6 0.0 4.1 2.023729 65863 66963 1180-1213 1.000000 75.8 petA-psbJ_p3 petA-psbJ 3 TGAGAAGGTTCAATTGTCCGA ATTCCGGATTGGGTTCATCC 21 20 42.9 50.0 57.2 57.6 5.1 4.1 2.127380 65863 66963 1128-1161 1.000000 75.0 petA-psbJ_p4 petA-psbJ 4 TTGGCTTCTGTTATTTTGGCAC ATTCCGGATTGGGTTCATCC 22 20 40.9 50.0 58.0 57.6 0.0 4.1 2.376154 65863 66963 1181-1214 1.000000 73.0 petA-psbJ_p5 petA-psbJ 5 TGAGAAGGTTCAATTGTCCGAA ATTCCGGATTGGGTTCATCC 22 20 40.9 50.0 57.9 57.6 5.1 4.1 2.444952 65863 66963 1128-1161 1.000000 72.4 psaJ-rpl33_p1 psaJ-rpl33 1 GGGAATGCTCGGGAGTAAAG GGTCGACTCGGTTCTTTCAA 20 20 55.0 50.0 58.0 57.9 0.0 16.5 0.189555 70321 70799 1418-1437 0.800000 75.3 psaJ-rpl33_p2 psaJ-rpl33 2 TGCGCTTACATTCCCCTTTT CTGTTCCTTGGAAGGGTGAC 20 20 45.0 55.0 58.1 58.1 9.7 5.6 0.184568 70321 70799 742-748 0.400000 56.9 psaJ-rpl33_p3 psaJ-rpl33 3 GGGAATGCTCGGGAGTAAAG CTGTTCCTTGGAAGGGTGAC 20 20 55.0 55.0 58.0 58.1 0.0 5.6 0.147513 70321 70799 1027-1033 0.400000 56.8 psaJ-rpl33_p4 psaJ-rpl33 4 ACCCGTGACATTTTGTACCC CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.202195 70321 70799 1334-1334 0.400000 56.4 psaJ-rpl33_p5 psaJ-rpl33 5 CCCGTGACATTTTGTACCCA CTGTTCCTTGGAAGGGTGAC 20 20 50.0 55.0 58.1 58.1 0.0 5.6 0.203538 70321 70799 1333-1333 0.400000 56.4 psbT-psbN_p1 psbT-psbN 1 TTCTTCGGACACATTTGGCA TCCCCTACGGTAGTTGGTTT 20 20 45.0 50.0 58.0 58.0 0.0 13.8 0.054296 77531 77593 794-799 1.000000 84.0 psbT-psbN_p2 psbT-psbN 2 TCCGGATTTGGATGCTCAAG TCCCCTACGGTAGTTGGTTT 20 20 50.0 50.0 58.0 58.0 0.0 13.8 0.071247 77531 77593 732-737 1.000000 84.0 psbT-psbN_p3 psbT-psbN 3 CGATCCCGCTACTGTGAAAA TCCCCTACGGTAGTTGGTTT 20 20 50.0 50.0 58.0 58.0 0.0 13.8 0.033144 77531 77593 945-950 1.000000 83.8 psbT-psbN_p4 psbT-psbN 4 CGATCCCGCTACTGTGAAAA AGCTAGGACTTCGTGGTTCT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.078474 77531 77593 1132-1137 0.800000 74.5 psbT-psbN_p5 psbT-psbN 5 TTCTTCGGACACATTTGGCA AGCTAGGACTTCGTGGTTCT 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.099627 77531 77593 981-986 0.800000 74.5 ndhF_p1 ndhF 1 ACTTCCGAAACGAAGGGAAC CGGTGAATCGGAAACAAGTA 20 20 50.0 45.0 58.1 55.8 0.0 0.0 2.320663 113433 115682 2474-2530 0.400000 38.7 ndhF_p2 ndhF 2 GAACTTCCGAAACGAAGGGA CGGTGAATCGGAAACAAGTA 20 20 50.0 45.0 57.8 55.8 0.0 0.0 2.354999 113433 115682 2476-2532 0.400000 38.4 ndhF_p3 ndhF 3 AGAACTTCCGAAACGAAGGG CGGTGAATCGGAAACAAGTA 20 20 50.0 45.0 57.8 55.8 0.0 0.0 2.360990 113433 115682 2477-2533 0.400000 38.4 ndhF_p4 ndhF 4 TAGGCAGAATACCCTCACCC CGGTGAATCGGAAACAAGTA 20 20 55.0 45.0 58.2 55.8 0.0 0.0 2.393118 113433 115682 2578-2634 0.400000 38.1 ndhF_p5 ndhF 5 GGAACAAGAGAGATCCACCG CGGTGAATCGGAAACAAGTA 20 20 55.0 45.0 57.7 55.8 0.0 0.0 2.497249 113433 115682 2448-2504 0.400000 37.3 ndhF-rpl32_p1 ndhF-rpl32 1 TTGGAAGTGGAAGGAAAGGT TCCAAAAATTTTTACGAATACGCT 20 24 45.0 29.2 56.6 56.2 0.0 15.5 7.242469 115683 116992 1303-1509 1.000000 50.1 ndhF-rpl32_p2 ndhF-rpl32 2 TTGGAAGTGGAAGGAAAGGT TCCAAAAATTTTTACGAATACGC 20 23 45.0 30.4 56.6 54.8 0.0 15.5 7.598854 115683 116992 1303-1509 1.000000 50.1 ndhF-rpl32_p3 ndhF-rpl32 3 TTGGAAGTGGAAGGAAAGGT TCCAAAAATTTTTACGAATACGCTT 20 25 45.0 28.0 56.6 56.8 0.0 15.5 7.606955 115683 116992 1303-1509 1.000000 50.1 ndhF-rpl32_p4 ndhF-rpl32 4 TTGGAAGTGGAAGGAAAGGT CCAAAAATTTTTACGAATACGCT 20 23 45.0 30.4 56.6 54.8 0.0 15.5 7.610673 115683 116992 1302-1508 1.000000 50.1 ndhF-rpl32_p5 ndhF-rpl32 5 TTGGAAGTGGAAGGAAAGGT TTCCAAAAATTTTTACGAATACGCT 20 25 45.0 28.0 56.6 56.8 0.0 15.5 7.606955 115683 116992 1304-1510 1.000000 50.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TTGGAAAAGGAAAGGACGGC AAGCACTGCTTCCTAAGAGC 20 20 50.0 50.0 58.7 57.9 0.0 10.7 0.782094 117182 118300 1301-1357 0.600000 63.1 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TTGGAAAAGGAAAGGACGGC GCTCAAGCACTGCTTCCTAA 20 20 50.0 50.0 58.7 58.2 0.0 13.6 0.862104 117182 118300 1305-1361 0.600000 62.5 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TTGGAAAAGGAAAGGACGGC CACTTGGCCCGGTTATTGTA 20 20 50.0 50.0 58.7 57.9 0.0 0.0 0.792444 117182 118300 1778-1844 0.600000 62.4 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TTGGAAAAGGAAAGGACGGC ACTTGGCCCGGTTATTGTAC 20 20 50.0 50.0 58.7 57.9 0.0 0.0 0.793930 117182 118300 1777-1843 0.600000 62.4 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TTGGAAAAGGAAAGGACGGC GCTTCCTAAGAGCAACGTGT 20 20 50.0 50.0 58.7 58.2 0.0 0.0 0.878593 117182 118300 1294-1350 0.600000 62.3 ccsA-ndhD_p1 ccsA-ndhD 1 TTCTGCAATTGTGGCGTCTA TGGGCGGAATAGCTATCTCA 20 20 45.0 50.0 57.8 58.0 7.4 6.7 0.187075 119489 119648 697-704 1.000000 84.9 ccsA-ndhD_p2 ccsA-ndhD 2 TACAATAACCGGGCCAAGTG TGGGCGGAATAGCTATCTCA 20 20 50.0 50.0 57.9 58.0 6.3 6.7 0.126508 119489 119648 1252-1259 1.000000 83.5 ccsA-ndhD_p3 ccsA-ndhD 3 GTACAATAACCGGGCCAAGT TGGGCGGAATAGCTATCTCA 20 20 50.0 50.0 57.9 58.0 6.3 6.7 0.127995 119489 119648 1253-1260 1.000000 83.5 ccsA-ndhD_p4 ccsA-ndhD 4 GCTAATGAGGCATGGGGATC TGGGCGGAATAGCTATCTCA 20 20 55.0 50.0 58.2 58.0 0.0 6.7 0.178101 119489 119648 819-826 0.800000 75.4 ccsA-ndhD_p5 ccsA-ndhD 5 CTAATGAGGCATGGGGATCG TGGGCGGAATAGCTATCTCA 20 20 55.0 50.0 57.9 58.0 0.0 6.7 0.114688 119489 119648 818-825 0.600000 66.5