primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	79	475	844-852	1.000000	83.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	79	475	843-851	1.000000	83.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	79	475	858-866	1.000000	83.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	79	475	857-865	1.000000	83.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	79	475	859-867	1.000000	83.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4357	5181	945-957	1.000000	81.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4357	5181	944-956	1.000000	81.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	20	20	45.0	45.0	57.8	58.0	0.0	7.6	0.224713	4357	5181	966-978	1.000000	81.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4357	5181	954-966	1.000000	80.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.2	58.2	20.3	7.6	0.397463	4357	5181	948-960	1.000000	80.1
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	AGGAATCAAACGAAAGATCCCT	20	22	45.0	40.9	58.0	57.4	0.0	0.0	2.614537	9498	10315	814-943	1.000000	64.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	GCTTTAGTCCACTCAGCCAT	AGGAATCAAACGAAAGATCCCT	20	22	50.0	40.9	57.6	57.4	0.9	0.0	2.986278	9498	10315	800-929	1.000000	61.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGAAGAAACGGAGAGAGGGG	AGGAATCAAACGAAAGATCCCT	20	22	55.0	40.9	58.4	57.4	0.0	0.0	3.013547	9498	10315	870-999	1.000000	61.0
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGTCCACTCAGCCATCTCTC	AGGAATCAAACGAAAGATCCCT	20	22	55.0	40.9	58.5	57.4	0.0	0.0	3.092292	9498	10315	795-924	1.000000	60.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	GGAATCAAACGAAAGATCCCT	20	21	45.0	42.9	58.0	55.9	0.0	0.0	3.096814	9498	10315	813-942	1.000000	60.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.204630	29043	30098	1134-1170	1.000000	82.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	CTGCAAATCCTTTCTCCCCA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.372001	29043	30098	1090-1126	1.000000	81.2
trnC-GCA-petN_p3	trnC-GCA-petN	3	TGCAAATCCTTTCTCCCCAG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.372001	29043	30098	1089-1125	1.000000	81.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	GCAAATCCTTTCTCCCCAGT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.373575	29043	30098	1088-1124	1.000000	81.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	ACTGCAAATCCTTTCTCCCC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.373575	29043	30098	1091-1127	1.000000	81.1
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.181932	37592	37805	336-337	1.000000	81.4
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.213831	37592	37805	329-330	1.000000	81.2
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	CCTGTTCTTTCCATGACCCC	AGCTCTGGTCTTGAAAACCG	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.276341	37592	37805	314-315	1.000000	80.8
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	20	20	55.0	55.0	58.2	58.2	0.0	17.2	0.377505	37592	37805	267-268	1.000000	80.6
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	20	20	45.0	55.0	58.4	58.0	38.2	0.0	0.397019	37592	37805	379-380	1.000000	79.4
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TGGTTGAAGATCGCAAGGAG	20	20	50.0	50.0	57.9	57.9	4.1	4.6	0.214484	44057	44695	663-751	1.000000	82.7
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GTTGAAGATCGCAAGGAGGT	20	20	50.0	50.0	57.9	57.9	4.1	4.6	0.215947	44057	44695	661-749	1.000000	82.7
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	GTCCGATCAAGCTGCTGAAT	20	20	50.0	50.0	57.9	58.3	4.1	1.4	0.454002	44057	44695	736-824	1.000000	80.8
psaA-ycf3_p4	psaA-ycf3	4	TGTTCTTGAGAAATGGCCGG	TGGTTGAAGATCGCAAGGAG	20	20	50.0	50.0	58.5	57.9	0.0	4.6	0.565516	44057	44695	688-776	1.000000	79.9
psaA-ycf3_p5	psaA-ycf3	5	TGTTCTTGAGAAATGGCCGG	GTTGAAGATCGCAAGGAGGT	20	20	50.0	50.0	58.5	57.9	0.0	4.6	0.566979	44057	44695	686-774	1.000000	79.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTTCACTAATCGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	4.4	0.0	0.320066	52601	54451	1987-2008	1.000000	78.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTAATCGGGGCCAAAACTC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	4.4	0.0	0.321560	52601	54451	1983-2004	1.000000	78.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CACTAATCGGGGCCAAAACT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	4.4	0.0	0.363095	52601	54451	1984-2005	1.000000	78.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCACTAATCGGGGCCAAAAC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	4.4	0.0	0.368304	52601	54451	1985-2006	1.000000	78.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTTCACTAATCGGGGCCAAA	ACGCATGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	4.4	0.0	0.436429	52601	54451	2053-2074	1.000000	77.7
ycf4-cemA_p1	ycf4-cemA	1	CATTCCCTTGACCCGTACTG	AGATCCACCAGGGCAAAAAG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.095307	63557	64467	1060-1088	1.000000	82.2
ycf4-cemA_p2	ycf4-cemA	2	CATTCCCTTGACCCGTACTG	GATCCACCAGGGCAAAAAGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.100820	63557	64467	1059-1087	1.000000	82.2
ycf4-cemA_p3	ycf4-cemA	3	CATTCCCTTGACCCGTACTG	GAGATCCACCAGGGCAAAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.100820	63557	64467	1061-1089	1.000000	82.1
ycf4-cemA_p4	ycf4-cemA	4	ATTCCCTTGACCCGTACTGA	AGATCCACCAGGGCAAAAAG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.124147	63557	64467	1059-1087	1.000000	82.0
ycf4-cemA_p5	ycf4-cemA	5	ATTCCCTTGACCCGTACTGA	GATCCACCAGGGCAAAAAGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	63557	64467	1058-1086	1.000000	81.9
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	58.2	58.2	0.0	4.1	0.344636	66343	67502	1271-1388	1.000000	84.8
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	20	20	45.0	55.0	58.2	58.6	0.0	4.1	0.728252	66343	67502	1272-1389	1.000000	81.7
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.030186	66343	67502	1270-1387	1.000000	79.3
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	66343	67502	1264-1381	1.000000	79.1
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	57.1	58.2	0.0	4.1	1.105127	66343	67502	1270-1387	1.000000	78.7
rps19-rpl2_p1	rps19-rpl2	1	GCCCGGGACCAAGTTACTAT	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	59.2	58.0	29.3	22.4	1.188900	87755	87833	261-271	1.000000	76.2
rps19-rpl2_p2	rps19-rpl2	2	GCCCGGGACCAAGTTACTAT	CCTGCGCTTGGAAGAAAAAG	20	20	55.0	50.0	59.2	57.9	29.3	9.7	1.220818	87755	87833	256-266	1.000000	76.1
rps19-rpl2_p3	rps19-rpl2	3	GCCCGGGACCAAGTTACTAT	ATCCTGCGCTTGGAAGAAAA	20	20	55.0	45.0	59.2	58.1	29.3	22.4	1.255024	87755	87833	258-268	1.000000	75.8
rps19-rpl2_p4	rps19-rpl2	4	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	59.2	58.2	29.3	35.0	1.317396	87755	87833	275-285	1.000000	74.9
rps19-rpl2_p5	rps19-rpl2	5	GCCCGGGACCAAGTTACTAT	GGCCCCGATTGGTAGAAAAA	20	20	55.0	50.0	59.2	58.2	29.3	0.0	1.322672	87755	87833	299-309	1.000000	74.7
rpl2-trnI-CAU_p1	rpl2-trnI-CAU	1	TGGATCCTTTGCGTATGCTC	ACTCGCGATGTACAATGACC	20	20	50.0	50.0	58.0	58.1	14.2	10.4	0.123871	89332	89747	559-570	1.000000	83.0
rpl2-trnI-CAU_p2	rpl2-trnI-CAU	2	TGGATCCTTTGCGTATGCTC	GGTCACTCGCGATGTACAAT	20	20	50.0	50.0	58.0	58.1	14.2	10.4	0.123871	89332	89747	563-574	1.000000	83.0
rpl2-trnI-CAU_p3	rpl2-trnI-CAU	3	TGGATCCTTTGCGTATGCTC	AATGACCCCAGTGAAGCATC	20	20	50.0	50.0	58.0	57.9	14.2	0.0	0.181230	89332	89747	546-557	1.000000	82.6
rpl2-trnI-CAU_p4	rpl2-trnI-CAU	4	TGGATCCTTTGCGTATGCTC	CAATGACCCCAGTGAAGCAT	20	20	50.0	50.0	58.0	58.2	14.2	0.0	0.203073	89332	89747	547-558	1.000000	82.4
rpl2-trnI-CAU_p5	rpl2-trnI-CAU	5	TGCTCGGGGTAGAAGTTTTG	ACTCGCGATGTACAATGACC	20	20	50.0	50.0	57.8	58.1	0.0	10.4	0.256342	89332	89747	544-555	1.000000	82.0
ycf2_p1	ycf2	1	TCACCAATTGAGTCACAGGT	ACGGAGATAGTGATGCAAGT	20	20	45.0	45.0	56.7	56.3	7.1	0.0	2.973574	93280	93399	261-261	1.000000	61.1
ycf2_p2	ycf2	2	TTGAGTCACAGGTATCTGGC	ACGGAGATAGTGATGCAAGT	20	20	50.0	45.0	56.9	56.3	8.1	0.0	2.710000	93280	93399	254-254	0.967742	61.1
ycf2_p3	ycf2	3	TTGAGTCACAGGTATCTGGC	TGAAACGGAGATAGTGATGCA	20	21	50.0	42.9	56.9	57.1	8.1	0.0	2.945012	93280	93399	258-258	0.967742	59.2
ycf2_p4	ycf2	4	TTGAGTCACAGGTATCTGGC	AACGGAGATAGTGATGCAAGT	20	21	50.0	42.9	56.9	57.1	8.1	0.0	2.954520	93280	93399	255-255	0.967742	59.2
ycf2_p5	ycf2	5	TTGAGTCACAGGTATCTGGC	ACGGAGATAGTGATGCAAGTT	20	21	50.0	42.9	56.9	57.1	8.1	0.0	2.954520	93280	93399	254-254	0.967742	59.2
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.323995	116093	117214	1234-1347	1.000000	81.2
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.332664	116093	117214	1228-1341	1.000000	81.1
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.562944	116093	117214	1229-1342	1.000000	79.3
ndhF-rpl32_p4	ndhF-rpl32	4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.568384	116093	117214	1292-1405	1.000000	79.1
ndhF-rpl32_p5	ndhF-rpl32	5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.577053	116093	117214	1286-1399	1.000000	79.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.7	57.9	13.1	0.0	0.386849	117389	118773	1517-1525	1.000000	81.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.7	57.8	13.1	0.0	0.447576	117389	118773	1510-1518	1.000000	80.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.7	57.8	13.1	0.0	0.458867	117389	118773	1516-1524	1.000000	80.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.7	57.8	13.1	0.0	0.458867	117389	118773	1515-1523	1.000000	80.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	57.7	58.3	13.1	0.0	0.535719	117389	118773	1511-1519	1.000000	79.9
ycf1_p1	ycf1	1	TTGCCTCTTCCTCCTCAGAA	TGGCTTTGGTACGTTACTCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.098864	131707	131826	255-264	1.000000	84.2
ycf1_p2	ycf1	2	TTTGCCTCTTCCTCCTCAGA	TGGCTTTGGTACGTTACTCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.098864	131707	131826	256-265	1.000000	84.2
ycf1_p3	ycf1	3	TTGCCTCTTCCTCCTCAGAA	TACGCGCTCAAAGACGTAAA	20	20	50.0	45.0	58.0	58.0	0.0	5.7	0.079104	131707	131826	195-204	0.967742	83.4
ycf1_p4	ycf1	4	TTTGCCTCTTCCTCCTCAGA	TACGCGCTCAAAGACGTAAA	20	20	50.0	45.0	58.0	58.0	0.0	5.7	0.079104	131707	131826	196-205	0.967742	83.4
ycf1_p5	ycf1	5	TTGCCTCTTCCTCCTCAGAA	GATCCATACGCGCTCAAAGA	20	20	50.0	50.0	58.0	58.1	0.0	5.7	0.177085	131707	131826	201-210	0.967742	82.5