primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	TATCAAACGATCCTGGACGG	TTACTTGGAGAGATGGCCGA	20	20	50.0	50.0	57.1	58.1	18.2	0.0	1.022286	8260	8323	199-199	1.000000	72.2
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TATCAAACGATCCTGGACGG	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.1	58.2	18.2	17.2	1.099856	8260	8323	128-128	1.000000	71.8
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	TATCAAACGATCCTGGACGG	TAACGCGTCGGATTGCTAAT	20	20	50.0	45.0	57.1	57.8	18.2	14.4	1.095796	8260	8323	173-173	1.000000	71.7
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	TATCAAACGATCCTGGACGG	CCATATGTACCGAGGGTTCG	20	20	50.0	55.0	57.1	57.6	18.2	1.2	1.316796	8260	8323	141-141	1.000000	70.0
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	TTATCAAACGATCCTGGACGG	TTACTTGGAGAGATGGCCGA	21	20	47.6	50.0	57.8	58.1	18.2	0.0	1.313027	8260	8323	200-200	1.000000	69.9
psbM-petN_p1	psbM-petN	1	TTCGTAGCCACTGCACTTTT	CTGCAGTTTGGGGAAGAAGT	20	20	45.0	50.0	58.0	58.0	0.0	5.2	0.051324	29204	30649	1556-1559	1.000000	80.3
psbM-petN_p2	psbM-petN	2	TTCGTAGCCACTGCACTTTT	GTTTGGGGAAGAAGTGGGTT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.120347	29204	30649	1551-1554	1.000000	79.7
psbM-petN_p3	psbM-petN	3	TTCGTAGCCACTGCACTTTT	TGCAGTTTGGGGAAGAAGTG	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.347010	29204	30649	1555-1558	1.000000	77.9
psbM-petN_p4	psbM-petN	4	TTCGTAGCCACTGCACTTTT	CAGTTTGGGGAAGAAGTGGG	20	20	45.0	55.0	58.0	58.4	0.0	0.0	0.417384	29204	30649	1553-1556	1.000000	77.3
psbM-petN_p5	psbM-petN	5	ACATTCAAACGGTCGCTCAA	CTGCAGTTTGGGGAAGAAGT	20	20	45.0	50.0	58.4	58.0	0.0	5.2	0.424443	29204	30649	1501-1504	1.000000	77.3
trnC-GCA-trnG-GCC_p1	trnC-GCA-trnG-GCC	1	AGGATTTGCAATCCTCCGTC	TGATGTGGTGTAGAATGCGG	20	20	50.0	50.0	58.0	58.0	19.0	0.0	0.066887	31216	31797	725-725	1.000000	80.2
trnC-GCA-trnG-GCC_p2	trnC-GCA-trnG-GCC	2	GGATTTGCAATCCTCCGTCT	TGATGTGGTGTAGAATGCGG	20	20	50.0	50.0	58.0	58.0	16.2	0.0	0.066887	31216	31797	724-724	1.000000	80.2
trnC-GCA-trnG-GCC_p3	trnC-GCA-trnG-GCC	3	AGGATTTGCAATCCTCCGTC	GATGTGGTGTAGAATGCGGT	20	20	50.0	50.0	58.0	58.0	19.0	0.0	0.068298	31216	31797	724-724	1.000000	80.2
trnC-GCA-trnG-GCC_p4	trnC-GCA-trnG-GCC	4	GGATTTGCAATCCTCCGTCT	GATGTGGTGTAGAATGCGGT	20	20	50.0	50.0	58.0	58.0	16.2	0.0	0.068298	31216	31797	723-723	1.000000	80.2
trnC-GCA-trnG-GCC_p5	trnC-GCA-trnG-GCC	5	TCCGTCTTACCGCTTGACTA	TGATGTGGTGTAGAATGCGG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.197674	31216	31797	711-711	1.000000	79.2
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	AGAGATGGTCGAGTGGTTCA	TTCTTTTTCTTCGTGGGCCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.135319	32697	33461	969-972	1.000000	79.9
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GGGTTCGAATCCCTCTCTCT	TTCTTTTTCTTCGTGGGCCA	20	20	55.0	45.0	58.2	57.9	17.2	0.0	0.276979	32697	33461	905-908	1.000000	78.8
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	AAAACTGGCATGGTGTCGAA	TTCTTTTTCTTCGTGGGCCA	20	20	45.0	45.0	58.3	57.9	0.0	0.0	0.373695	32697	33461	935-938	1.000000	78.0
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	AAACTGGCATGGTGTCGAAA	TTCTTTTTCTTCGTGGGCCA	20	20	45.0	45.0	58.3	57.9	0.0	0.0	0.373695	32697	33461	934-937	1.000000	78.0
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	GATGGTCGAGTGGTTCATGG	TTCTTTTTCTTCGTGGGCCA	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.403398	32697	33461	966-969	1.000000	77.8
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	AGACCAGACCAACCTACGAA	GGCCCTTTTAACTCAGTGGT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.293348	35893	37462	1750-1750	1.000000	78.0
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TTTTCCAATGGCGATGGTCA	GGCCCTTTTAACTCAGTGGT	20	20	45.0	50.0	58.1	57.7	8.1	0.0	0.371472	35893	37462	1664-1664	1.000000	77.4
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	TTTCCAATGGCGATGGTCAT	GGCCCTTTTAACTCAGTGGT	20	20	45.0	50.0	57.9	57.7	8.1	0.0	0.430333	35893	37462	1663-1663	1.000000	76.9
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	AGACCAGACCAACCTACGAA	AGGCCCTTTTAACTCAGTGG	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.592019	35893	37462	1751-1751	1.000000	75.6
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	TTTTCCAATGGCGATGGTCA	AGGCCCTTTTAACTCAGTGG	20	20	45.0	50.0	58.1	57.4	8.1	0.0	0.670143	35893	37462	1665-1665	1.000000	75.0
rps4-trnL-CAA_p1	rps4-trnL-CAA	1	CCTGTAAGCCCGGGTAAATC	CTGAATCTGGGAGCTGTCAC	20	20	55.0	55.0	58.0	58.0	24.6	0.0	0.058183	47171	47848	834-836	1.000000	80.0
rps4-trnL-CAA_p2	rps4-trnL-CAA	2	CTGTAAGCCCGGGTAAATCC	CTGAATCTGGGAGCTGTCAC	20	20	55.0	55.0	58.0	58.0	24.6	0.0	0.058183	47171	47848	833-835	1.000000	80.0
rps4-trnL-CAA_p3	rps4-trnL-CAA	3	ATGCTTTACCTGTAAGCCCG	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.059742	47171	47848	842-844	1.000000	80.0
rps4-trnL-CAA_p4	rps4-trnL-CAA	4	TACCTGTAAGCCCGGGTAAA	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.1	58.0	24.6	0.0	0.071556	47171	47848	836-838	1.000000	79.9
rps4-trnL-CAA_p5	rps4-trnL-CAA	5	TTTACCTGTAAGCCCGGGTA	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.1	58.0	24.6	0.0	0.071556	47171	47848	838-840	1.000000	79.9
accD-psaI_p1	accD-psaI	1	GAAGGGTGTTTTGAGCGAGA	AGGGTAGATAGGACGCTGTC	20	20	50.0	55.0	58.1	58.0	0.0	0.8	0.136981	57811	58404	664-677	1.000000	81.9
accD-psaI_p2	accD-psaI	2	GAAGGGTGTTTTGAGCGAGA	GAGGGTAGATAGGACGCTGT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.136981	57811	58404	665-678	1.000000	81.9
accD-psaI_p3	accD-psaI	3	GAAGGGTGTTTTGAGCGAGA	TGTAATTGCCGGAAACACCA	20	20	50.0	45.0	58.1	58.0	0.0	5.0	0.138387	57811	58404	708-721	1.000000	81.7
accD-psaI_p4	accD-psaI	4	CTGAGCCTTATTCCTCAGCC	AGGGTAGATAGGACGCTGTC	20	20	55.0	55.0	57.7	58.0	4.9	0.8	0.268911	57811	58404	641-654	1.000000	80.9
accD-psaI_p5	accD-psaI	5	CTGAGCCTTATTCCTCAGCC	GAGGGTAGATAGGACGCTGT	20	20	55.0	55.0	57.7	58.0	4.9	0.0	0.268911	57811	58404	642-655	1.000000	80.9
petA-psbJ_p1	petA-psbJ	1	AAAAAGTCCAGCTGGCAGAA	GTTCGGTGGGCATATCCTTT	20	20	45.0	50.0	57.9	57.9	28.9	0.0	0.124560	62330	63044	802-803	1.000000	79.6
petA-psbJ_p2	petA-psbJ	2	AAAAGTCCAGCTGGCAGAAA	GTTCGGTGGGCATATCCTTT	20	20	45.0	50.0	57.9	57.9	28.9	0.0	0.124560	62330	63044	801-802	1.000000	79.6
petA-psbJ_p3	petA-psbJ	3	TTGAAAAAGTCCAGCTGGCA	GTTCGGTGGGCATATCCTTT	20	20	45.0	50.0	58.2	57.9	28.9	0.0	0.278672	62330	63044	805-806	1.000000	78.4
petA-psbJ_p4	petA-psbJ	4	AAAGTCCAGCTGGCAGAAAT	GTTCGGTGGGCATATCCTTT	20	20	45.0	50.0	57.7	57.9	28.9	0.0	0.352108	62330	63044	800-801	1.000000	77.8
petA-psbJ_p5	petA-psbJ	5	AAAAGTCCAGCTGGCAGAAA	GAGCCTATTCGGGGTTAGGA	20	20	45.0	55.0	57.9	58.3	28.9	0.2	0.358480	62330	63044	777-778	1.000000	77.7
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	CGAGGAGTATGTACCAGGGT	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.182695	67371	68404	1145-1147	1.000000	78.9
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	CTGGGGAGTGGGACAAAATC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.274437	67371	68404	1193-1195	1.000000	78.1
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	CAACGGCGAGGAGTATGTAC	20	20	50.0	55.0	57.9	58.2	0.0	16.6	0.277477	67371	68404	1151-1153	1.000000	78.1
rpl20-rps12_p4	rpl20-rps12	4	TGAATGAGCCCCTCGAAATC	CGAGGAGTATGTACCAGGGT	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.400897	67371	68404	1143-1145	1.000000	77.1
rpl20-rps12_p5	rpl20-rps12	5	AATCTTGAATGAGCCCCTCG	CGAGGAGTATGTACCAGGGT	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.407411	67371	68404	1148-1150	1.000000	77.1
rps3-rpl22_p1	rps3-rpl22	1	GGATGAATCTTTCGCCCCAT	CCGATAAGGAAACCCACCTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051504	82585	82671	182-182	1.000000	81.3
rps3-rpl22_p2	rps3-rpl22	2	AGTGGATGAATCTTTCGCCC	CCGATAAGGAAACCCACCTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.082510	82585	82671	185-185	1.000000	81.1
rps3-rpl22_p3	rps3-rpl22	3	ATCTTCCATCTGTGCGAACC	CCGATAAGGAAACCCACCTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.068225	82585	82671	246-246	1.000000	80.8
rps3-rpl22_p4	rps3-rpl22	4	GGATGAATCTTTCGCCCCAT	AAAAGGTTTCGTCCTCGGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138109	82585	82671	218-218	1.000000	80.4
rps3-rpl22_p5	rps3-rpl22	5	ATCTTCCATCTGTGCGAACC	AAAAGGTTTCGTCCTCGGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.154829	82585	82671	282-282	1.000000	79.9
ndhB-trnR-ACG_p1	ndhB-trnR-ACG	1	TCGGGATCACACAGAATACCT	AAAAGTGGTTCCTGGAACGG	21	20	47.6	50.0	58.3	58.3	0.0	28.1	1.565260	86718	88554	1974-2043	1.000000	68.8
ndhB-trnR-ACG_p2	ndhB-trnR-ACG	2	TCGGGATCACACAGAATACC	AAAAGTGGTTCCTGGAACGG	20	20	50.0	50.0	56.7	58.3	0.0	28.1	1.583138	86718	88554	1974-2043	1.000000	68.7
ndhB-trnR-ACG_p3	ndhB-trnR-ACG	3	CGGGATCACACAGAATACCT	AAAAGTGGTTCCTGGAACGG	20	20	50.0	50.0	56.7	58.3	0.0	28.1	1.592097	86718	88554	1973-2042	1.000000	68.6
ndhB-trnR-ACG_p4	ndhB-trnR-ACG	4	CGGGATCACACAGAATACCTT	AAAAGTGGTTCCTGGAACGG	21	20	47.6	50.0	57.5	58.3	0.0	28.1	1.856117	86718	88554	1973-2042	1.000000	66.5
ndhB-trnR-ACG_p5	ndhB-trnR-ACG	5	CCATTATCGGGATCACACAGA	AAAAGTGGTTCCTGGAACGG	21	20	47.6	50.0	57.2	58.3	0.0	28.1	2.063916	86718	88554	1980-2049	1.000000	64.8
rrn23_p1	rrn23	1	ACTTCTGTCGTTTCACGCTT	TAGTAGCACGTTGAAGTGGC	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.138614	89499	92315	2944-2945	1.000000	79.3
rrn23_p2	rrn23	2	TCGGGTTCTCGAATCAGAGA	TAGTAGCACGTTGAAGTGGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.198824	89499	92315	2963-2964	1.000000	78.8
rrn23_p3	rrn23	3	TCTCGGGTTCTCGAATCAGA	TAGTAGCACGTTGAAGTGGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.198824	89499	92315	2965-2966	1.000000	78.8
rrn23_p4	rrn23	4	CCCAGCTTTTTCTGCGTCTA	TAGTAGCACGTTGAAGTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.266136	89499	92315	2898-2899	1.000000	78.3
rrn23_p5	rrn23	5	CGGGTTCTCGAATCAGAGAC	TAGTAGCACGTTGAAGTGGC	20	20	55.0	50.0	57.8	57.9	4.7	0.0	0.270842	89499	92315	2962-2963	1.000000	78.2
rrn16-rps12_p1	rrn16-rps12	1	CATCCCCGTTAGCCTAGAGA	CCTGAGCGATTACGAACCAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.007784	101141	101390	786-1149	1.000000	84.7
rrn16-rps12_p2	rrn16-rps12	2	CATCCCCGTTAGCCTAGAGA	AAGTAGATCCTGTCCCTCCG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.077930	101141	101390	589-968	1.000000	84.2
rrn16-rps12_p3	rrn16-rps12	3	CATCCCCGTTAGCCTAGAGA	GACGAGCGGGTTAGAAAGAG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.092514	101141	101390	837-1200	1.000000	84.0
rrn16-rps12_p4	rrn16-rps12	4	GGTAGTGCCCTGATTGAGTG	CCTGAGCGATTACGAACCAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.034421	101141	101390	1006-1006	0.500000	59.7
rrn16-rps12_p5	rrn16-rps12	5	GTAGTGCCCTGATTGAGTGG	CCTGAGCGATTACGAACCAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.034421	101141	101390	1005-1005	0.500000	59.7
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	GGAGGGCTAAATCGGTGAAA	CTTGCATGGAGGTAACGGAA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.521260	118033	119184	1360-1363	1.000000	76.3
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	TGGAGGGCTAAATCGGTGAA	CTTGCATGGAGGTAACGGAA	20	20	50.0	50.0	58.7	57.9	0.0	0.0	0.826531	118033	119184	1361-1364	1.000000	73.9
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	GGAGGGCTAAATCGGTGAAA	TACTTGCATGGAGGTAACGG	20	20	50.0	50.0	57.6	57.3	0.0	6.7	1.097311	118033	119184	1362-1365	1.000000	71.7
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	TGGAGGGCTAAATCGGTGA	CTTGCATGGAGGTAACGGAA	19	20	52.6	50.0	58.0	57.9	0.0	0.0	1.107952	118033	119184	1361-1364	1.000000	71.6
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	GAGGGCTAAATCGGTGAAAGT	CTTGCATGGAGGTAACGGAA	21	20	47.6	50.0	58.0	57.9	0.0	0.0	1.120718	118033	119184	1359-1362	1.000000	71.5
chlN_p1	chlN	1	ACTCGGCAATTTCAAAACAA	TTCTGGAAAAGGGGAGGACA	20	20	35.0	50.0	54.6	58.2	0.0	0.0	3.570102	134535	135929	1546-1546	1.000000	51.7
chlN_p2	chlN	2	ACTCGGCAATTTCAAAACAA	AAGGGGAGGACACTCGAAAT	20	20	35.0	50.0	54.6	58.3	0.0	0.0	3.740090	134535	135929	1538-1538	1.000000	50.3
chlN_p3	chlN	3	ACTCGGCAATTTCAAAACAAA	TTCTGGAAAAGGGGAGGACA	21	20	33.3	50.0	55.4	58.2	0.0	0.0	3.740505	134535	135929	1546-1546	1.000000	50.3
chlN_p4	chlN	4	ACTCGGCAATTTCAAAACAAA	AAGGGGAGGACACTCGAAAT	21	20	33.3	50.0	55.4	58.3	0.0	0.0	3.910493	134535	135929	1538-1538	1.000000	49.0
chlN_p5	chlN	5	ACTCGGCAATTTCAAAACAA	AGGGGAGGACACTCGAAATA	20	20	35.0	50.0	54.6	57.5	0.0	0.0	3.942738	134535	135929	1537-1537	1.000000	48.7
rps12-rrn16_p1	rps12-rrn16	1	GGGACAGTGTCGATAGTGTC	ACCCGTTTGCTTGATGTCTC	20	20	55.0	50.0	57.4	58.5	31.1	0.0	1.039713	150239	150638	188-567	1.000000	76.7
rps12-rrn16_p2	rps12-rrn16	2	GGGACAGTGTCGATAGTGTC	CCCGTTTGCTTGATGTCTCA	20	20	55.0	50.0	57.4	58.5	31.1	0.0	1.040802	150239	150638	187-566	1.000000	76.7
rps12-rrn16_p3	rps12-rrn16	3	GGGACAGTGTCGATAGTGTC	TTAGACAGAACAGCCAACGC	20	20	55.0	50.0	57.4	58.5	31.1	0.0	1.054839	150239	150638	166-545	1.000000	76.7
rps12-rrn16_p4	rps12-rrn16	4	GGGACAGTGTCGATAGTGTC	CCGTTTGCTTGATGTCTCAC	20	20	55.0	50.0	57.4	57.4	31.1	0.0	1.164951	150239	150638	186-565	1.000000	75.7
rps12-rrn16_p5	rps12-rrn16	5	GGGGACAGTGTCGATAGTGT	ACCCGTTTGCTTGATGTCTC	20	20	55.0	50.0	58.8	58.5	21.0	0.0	1.292694	150239	150638	189-568	1.000000	74.7
trnR-ACG-ndhB_p1	trnR-ACG-ndhB	1	AAAAGTGGTTCCTGGAACGG	TCGGGATCACACAGAATACCT	20	21	50.0	47.6	58.3	58.3	28.1	0.0	1.565260	163139	164975	1974-2043	1.000000	68.8
trnR-ACG-ndhB_p2	trnR-ACG-ndhB	2	AAAAGTGGTTCCTGGAACGG	TCGGGATCACACAGAATACC	20	20	50.0	50.0	58.3	56.7	28.1	0.0	1.583138	163139	164975	1974-2043	1.000000	68.7
trnR-ACG-ndhB_p3	trnR-ACG-ndhB	3	AAAAGTGGTTCCTGGAACGG	CGGGATCACACAGAATACCT	20	20	50.0	50.0	58.3	56.7	28.1	0.0	1.592097	163139	164975	1973-2042	1.000000	68.6
trnR-ACG-ndhB_p4	trnR-ACG-ndhB	4	AAAAGTGGTTCCTGGAACGG	CGGGATCACACAGAATACCTT	20	21	50.0	47.6	58.3	57.5	28.1	0.0	1.856117	163139	164975	1973-2042	1.000000	66.5
trnR-ACG-ndhB_p5	trnR-ACG-ndhB	5	AAAAGTGGTTCCTGGAACGG	CCATTATCGGGATCACACAGA	20	21	50.0	47.6	58.3	57.2	28.1	0.0	2.063916	163139	164975	1980-2049	1.000000	64.8