primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	20	20	45.0	50.0	58.0	58.2	0.0	5.0	0.209227	8006	8989	1128-1129	1.000000	79.0
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	8006	8989	1115-1116	1.000000	78.4
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	20	20	50.0	50.0	57.6	58.2	0.9	5.0	0.580969	8006	8989	1114-1115	1.000000	76.1
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	20	20	45.0	55.0	58.0	58.6	0.0	0.0	0.605692	8006	8989	1107-1108	1.000000	75.9
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.6	58.2	0.9	0.0	0.656018	8006	8989	1101-1102	1.000000	75.5
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	17935	18667	895-895	1.000000	68.0
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	21	20	42.9	60.0	57.6	58.3	0.0	0.0	1.689144	17935	18667	839-839	1.000000	67.0
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	21	20	42.9	55.0	57.6	58.4	0.0	0.0	1.779576	17935	18667	838-838	1.000000	66.2
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	56.1	58.2	0.0	0.0	2.081320	17935	18667	895-895	1.000000	63.8
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	AGTAGTATGGGGGAGGAGTG	21	20	42.9	55.0	57.6	57.2	0.0	0.0	2.184882	17935	18667	840-840	1.000000	63.0
atpH-atpF_p1	atpH-atpF	1	AGTTGTGGCACTAGCACTTT	GGATGGCCAGTGAGCTAAAA	20	20	45.0	50.0	57.7	57.9	19.8	13.0	0.477181	33389	33842	545-545	1.000000	77.2
atpH-atpF_p2	atpH-atpF	2	AGTTGTGGCACTAGCACTTT	ATTAAGCCCGAAACTCCCAG	20	20	45.0	50.0	57.7	57.6	19.8	0.0	0.768469	33389	33842	566-566	1.000000	74.8
atpH-atpF_p3	atpH-atpF	3	CTAGTTGTGGCACTAGCACT	GGATGGCCAGTGAGCTAAAA	20	20	50.0	50.0	57.2	57.9	19.8	13.0	0.879937	33389	33842	547-547	1.000000	74.0
atpH-atpF_p4	atpH-atpF	4	CTAGTTGTGGCACTAGCACT	ATTAAGCCCGAAACTCCCAG	20	20	50.0	50.0	57.2	57.6	19.8	0.0	1.171225	33389	33842	568-568	1.000000	71.6
atpH-atpF_p5	atpH-atpF	5	CTAGTTGTGGCACTAGCACTT	GGATGGCCAGTGAGCTAAAA	21	20	47.6	50.0	58.0	57.9	19.8	13.0	1.174371	33389	33842	547-547	1.000000	71.6
psaA-ycf3_p1	psaA-ycf3	1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.480902	42376	42992	798-800	1.000000	77.2
psaA-ycf3_p2	psaA-ycf3	2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.5	58.0	0.0	18.7	0.499445	42376	42992	726-728	1.000000	77.1
psaA-ycf3_p3	psaA-ycf3	3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.522343	42376	42992	794-796	1.000000	76.8
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540887	42376	42992	722-724	1.000000	76.8
psaA-ycf3_p5	psaA-ycf3	5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.7	58.0	0.0	18.7	0.667465	42376	42992	779-781	1.000000	75.7
rps4_p1	rps4	1	AGGTCGATTTAGTTCGGGGA	TCAGTTTTAATTTCGATGTATTCTGT	20	26	50.0	26.9	58.1	55.8	0.0	0.0	8.370216	45923	46528	759-759	1.000000	40.5
rps4_p2	rps4	2	CGGGGAAAGGGGAAGAAAAT	TCAGTTTTAATTTCGATGTATTCTGT	20	26	50.0	26.9	57.8	55.8	0.0	0.0	8.458561	45923	46528	745-745	1.000000	40.5
rps4_p3	rps4	3	TTAGTTCGGGGAAAGGGGAA	TCAGTTTTAATTTCGATGTATTCTGT	20	26	50.0	26.9	58.3	55.8	0.0	0.0	8.481973	45923	46528	751-751	1.000000	40.5
rps4_p4	rps4	4	TTTAGTTCGGGGAAAGGGGA	TCAGTTTTAATTTCGATGTATTCTGT	20	26	50.0	26.9	58.3	55.8	0.0	0.0	8.481973	45923	46528	752-752	1.000000	40.5
rps4_p5	rps4	5	AGGTCGATTTAGTTCGGGGA	CAGTTTTAATTTCGATGTATTCTGT	20	25	50.0	28.0	58.1	54.5	0.0	0.0	8.658258	45923	46528	758-758	1.000000	40.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	46904	47604	775-792	1.000000	80.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.1	58.2	0.0	10.2	0.313860	46904	47604	767-784	1.000000	80.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46904	47604	745-762	1.000000	80.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.434961	46904	47604	769-786	1.000000	79.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CGATGACCCTCGCATTACAA	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.476197	46904	47604	761-778	1.000000	79.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.0	57.9	6.3	0.0	0.110083	50931	51577	773-887	1.000000	79.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	58.0	58.3	6.3	22.8	0.339465	50931	51577	774-888	1.000000	77.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.3	57.9	6.3	0.0	0.415737	50931	51577	772-886	1.000000	77.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.5	57.9	6.3	0.0	0.560721	50931	51577	775-889	1.000000	75.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.3	58.3	6.3	22.8	0.645119	50931	51577	773-887	1.000000	75.2
rbcL-psaI_p1	rbcL-psaI	1	CGAGTTCGAGCCGGTAGATA	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.8	58.0	1.9	26.9	0.802928	56752	58049	1401-1402	1.000000	74.7
rbcL-psaI_p2	rbcL-psaI	2	CGAGTTCGAGCCGGTAGATA	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.8	57.9	1.9	26.8	0.879462	56752	58049	1407-1408	1.000000	74.1
rbcL-psaI_p3	rbcL-psaI	3	AGTTCGAGCCGGTAGATAAAC	CCATTGCAATTGCCGGAAAT	21	20	47.6	45.0	57.6	58.0	1.9	26.9	1.460516	56752	58049	1399-1400	1.000000	69.5
rbcL-psaI_p4	rbcL-psaI	4	GTTCGAGCCGGTAGATAAACT	CCATTGCAATTGCCGGAAAT	21	20	47.6	45.0	57.6	58.0	1.9	26.9	1.460516	56752	58049	1398-1399	1.000000	69.5
rbcL-psaI_p5	rbcL-psaI	5	GAGTTCGAGCCGGTAGATAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	56.6	58.0	1.9	26.9	1.464133	56752	58049	1400-1401	1.000000	69.4
rpl36-infA_p1	rpl36-infA	1	AACTCTAATTCGTCCACGCC	ATTCGAAGCGTACCGAAGAC	20	20	50.0	50.0	58.0	58.1	0.0	3.3	0.100521	76945	77055	270-272	1.000000	80.4
rpl36-infA_p2	rpl36-infA	2	AACTCTAATTCGTCCACGCC	GATTCGAAGCGTACCGAAGA	20	20	50.0	50.0	58.0	57.8	0.0	3.3	0.190355	76945	77055	271-273	1.000000	79.7
rpl36-infA_p3	rpl36-infA	3	AACTCTAATTCGTCCACGCC	CCCATAAGGATTCGAAGCGT	20	20	50.0	50.0	58.0	57.8	0.0	7.4	0.246464	76945	77055	279-281	1.000000	79.2
rpl36-infA_p4	rpl36-infA	4	AACTCTAATTCGTCCACGCC	TAAGGATTCGAAGCGTACCG	20	20	50.0	50.0	58.0	57.5	0.0	9.2	0.486194	76945	77055	275-277	1.000000	77.3
rpl36-infA_p5	rpl36-infA	5	AACTCTAATTCGTCCACGCC	CCCCATAAGGATTCGAAGCG	20	20	50.0	55.0	58.0	58.5	0.0	7.4	0.493045	76945	77055	280-282	1.000000	77.2
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	GCTGGATAAGATAAGGGGCG	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	57.9	58.0	0.0	22.4	0.124780	81965	82019	223-223	1.000000	79.4
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	81965	82019	205-205	1.000000	79.2
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	81965	82019	191-191	1.000000	79.2
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	81965	82019	190-190	1.000000	79.1
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	81965	82019	192-192	1.000000	79.1
rrn23-rrn4.5_p1	rrn23-rrn4.5	1	CCAAGTGCGGAGAGGATAAC	GTTCGTTCGTTAGGATGCCT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.064646	99909	100003	249-249	1.000000	80.1
rrn23-rrn4.5_p2	rrn23-rrn4.5	2	CCAAGTGCGGAGAGGATAAC	TCACTGCACTTCCACTTGAC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.105116	99909	100003	217-217	1.000000	79.9
rrn23-rrn4.5_p3	rrn23-rrn4.5	3	CCAAGTGCGGAGAGGATAAC	CAGGTCGTGTAGGAACAAGG	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.138961	99909	100003	277-277	1.000000	79.5
rrn23-rrn4.5_p4	rrn23-rrn4.5	4	AAGTAGTAAGCCCACCCCAA	GTTCGTTCGTTAGGATGCCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.255948	99909	100003	215-215	1.000000	78.7
rrn23-rrn4.5_p5	rrn23-rrn4.5	5	TAGTAAGCCCACCCCAAGAT	GTTCGTTCGTTAGGATGCCT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.286279	99909	100003	212-212	1.000000	78.5
rps15-ndhF_p1	rps15-ndhF	1	TCGGAAAGAGGTCTACGAAGA	AAATATGTAGGGGGAGGGCG	21	20	47.6	55.0	57.9	58.9	0.0	0.0	1.998686	102913	103369	646-664	1.000000	67.1
rps15-ndhF_p2	rps15-ndhF	2	ACTTTTGGGAAAACGTCAACG	AAATATGTAGGGGGAGGGCG	21	20	42.9	55.0	57.9	58.9	0.1	0.0	2.074778	102913	103369	626-644	1.000000	66.6
rps15-ndhF_p3	rps15-ndhF	3	TCGGAAAGAGGTCTACGAAGA	GTAGGGGGAGGGCGAATAT	21	19	47.6	57.9	57.9	57.9	0.0	0.0	2.147702	102913	103369	640-658	1.000000	65.9
rps15-ndhF_p4	rps15-ndhF	4	TCGGAAAGAGGTCTACGAAGA	GTAGGGGGAGGGCGAATATC	21	20	47.6	60.0	57.9	59.1	0.0	0.0	2.160274	102913	103369	640-658	1.000000	65.8
rps15-ndhF_p5	rps15-ndhF	5	ACTTTTGGGAAAACGTCAACG	GTAGGGGGAGGGCGAATAT	21	19	42.9	57.9	57.9	57.9	0.1	0.0	2.223794	102913	103369	620-638	1.000000	65.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGTTTTTCTGGGCAACAAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.824999	106619	107357	808-809	1.000000	74.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGTTTTTCTGGGCAACAAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.3	57.8	0.0	0.0	0.885726	106619	107357	801-802	1.000000	74.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGTTTTTCTGGGCAACAAA	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	57.3	58.2	0.0	4.7	0.887209	106619	107357	822-823	1.000000	74.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGTTTTTCTGGGCAACAAA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.3	57.8	0.0	0.0	0.897017	106619	107357	807-808	1.000000	74.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGTTTTTCTGGGCAACAAA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.3	57.8	0.0	0.0	0.897017	106619	107357	806-807	1.000000	74.2
ndhG_p1	ndhG	1	TGTTGGCAGTAGATGGGTTT	TCCCTTTTTCCTTCCTTGAATCT	20	23	45.0	39.1	57.0	57.7	0.0	0.0	4.309244	111603	112133	704-704	1.000000	45.8
ndhG_p2	ndhG	2	TTGTTGGCAGTAGATGGGTT	TCCCTTTTTCCTTCCTTGAATCT	20	23	45.0	39.1	57.0	57.7	0.0	0.0	4.309244	111603	112133	705-705	1.000000	45.8
ndhG_p3	ndhG	3	TTTGTTGGCAGTAGATGGGT	TCCCTTTTTCCTTCCTTGAATCT	20	23	45.0	39.1	57.0	57.7	0.0	0.0	4.309244	111603	112133	706-706	1.000000	45.8
ndhG_p4	ndhG	4	TGTTGGCAGTAGATGGGTTT	TCCTAATCCCTTTTTCCTTCCT	20	22	45.0	40.9	57.0	56.4	0.0	0.0	4.560016	111603	112133	710-710	1.000000	43.7
ndhG_p5	ndhG	5	TTGTTGGCAGTAGATGGGTT	TCCTAATCCCTTTTTCCTTCCT	20	22	45.0	40.9	57.0	56.4	0.0	0.0	4.560016	111603	112133	711-711	1.000000	43.7
ndhG-ndhI_p1	ndhG-ndhI	1	TCTCATGTATTGGTCCAGGT	GAGTCGGTTACCAATCTCCA	20	20	45.0	50.0	55.5	56.7	0.0	1.1	3.817106	112134	112365	378-379	1.000000	51.4
ndhG-ndhI_p2	ndhG-ndhI	2	TCTCATGTATTGGTCCAGGT	TGAGTCGGTTACCAATCTCC	20	20	45.0	50.0	55.5	56.7	0.0	1.1	3.817106	112134	112365	379-380	1.000000	51.4
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCCAAATAGCACAAGAATCT	GAGTCGGTTACCAATCTCCA	22	20	40.9	50.0	57.4	56.7	0.0	1.1	3.933131	112134	112365	397-398	1.000000	50.3
ndhG-ndhI_p4	ndhG-ndhI	4	TCCCCAAATAGCACAAGAATCT	TGAGTCGGTTACCAATCTCC	22	20	40.9	50.0	57.4	56.7	0.0	1.1	3.933131	112134	112365	398-399	1.000000	50.3
ndhG-ndhI_p5	ndhG-ndhI	5	TCTCATGTATTGGTCCAGGT	TGAGTCGGTTACCAATCTCCA	20	21	45.0	47.6	55.5	58.5	0.0	1.1	3.943525	112134	112365	379-380	1.000000	50.3
rrn4.5-rrn23_p1	rrn4.5-rrn23	1	GTTCGTTCGTTAGGATGCCT	CCAAGTGCGGAGAGGATAAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064646	119380	119474	249-249	1.000000	80.1
rrn4.5-rrn23_p2	rrn4.5-rrn23	2	TCACTGCACTTCCACTTGAC	CCAAGTGCGGAGAGGATAAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.105116	119380	119474	217-217	1.000000	79.9
rrn4.5-rrn23_p3	rrn4.5-rrn23	3	CAGGTCGTGTAGGAACAAGG	CCAAGTGCGGAGAGGATAAC	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.138961	119380	119474	277-277	1.000000	79.5
rrn4.5-rrn23_p4	rrn4.5-rrn23	4	GTTCGTTCGTTAGGATGCCT	AAGTAGTAAGCCCACCCCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.255948	119380	119474	215-215	1.000000	78.7
rrn4.5-rrn23_p5	rrn4.5-rrn23	5	GTTCGTTCGTTAGGATGCCT	TAGTAAGCCCACCCCAAGAT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.286279	119380	119474	212-212	1.000000	78.5
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	GCTGGATAAGATAAGGGGCG	20	20	50.0	55.0	58.0	57.9	22.4	0.0	0.124780	137364	137418	223-223	1.000000	79.4
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	137364	137418	205-205	1.000000	79.2
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	137364	137418	191-191	1.000000	79.2
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	137364	137418	190-190	1.000000	79.1
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	137364	137418	192-192	1.000000	79.1