primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 AATCCACTGCCTTAATCCGC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 58.3 57.9 0.0 0.0 0.394391 96 613 601-670 1.000000 79.5 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CCTTAATCCGCTTGGCTACA TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.7 57.9 0.0 0.0 0.400312 96 613 592-661 1.000000 79.5 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 CGCATGGTGGATTCACAATC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.5 57.9 6.4 0.0 0.554952 96 613 617-686 1.000000 78.2 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 TAATCCGCTTGGCTACATCC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.5 57.9 0.0 0.0 0.618506 96 613 589-658 1.000000 77.8 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 AATCCACTGCCTTAATCCGC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 58.3 58.3 0.0 0.0 0.655968 96 613 650-719 1.000000 77.3 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 TGCTTTCTACCACAGCGTTT TTTGGTCCCGCTATTCGAAG 20 20 45.0 50.0 58.0 58.0 0.0 3.3 0.056748 6647 8439 1807-1872 1.000000 84.5 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 TTGCTTTCTACCACAGCGTT TTTGGTCCCGCTATTCGAAG 20 20 45.0 50.0 58.0 58.0 0.0 3.3 0.056748 6647 8439 1808-1873 1.000000 84.5 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CAGATCCATCCACCAAGTCG TTTGGTCCCGCTATTCGAAG 20 20 55.0 50.0 58.1 58.0 0.0 3.3 0.073155 6647 8439 1832-1897 1.000000 84.3 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 AAGTCGCACGTTGCTTTCTA TTTGGTCCCGCTATTCGAAG 20 20 45.0 50.0 58.1 58.0 8.1 3.3 0.157866 6647 8439 1818-1883 1.000000 83.7 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 CCACAACAGATCCATCCACC TTTGGTCCCGCTATTCGAAG 20 20 55.0 50.0 58.2 58.0 0.0 3.3 0.263362 6647 8439 1838-1903 1.000000 82.8 atpH-atpI_p1 atpH-atpI 1 AACAGAAGCGGCAGAAATCA TCCCTGTCATGTTCCTTGGA 20 20 45.0 50.0 58.1 58.3 1.4 0.0 0.362752 15171 16260 1223-1225 1.000000 77.8 atpH-atpI_p2 atpH-atpI 2 TACCCTCTACAGCTTGACCC TCCCTGTCATGTTCCTTGGA 20 20 55.0 50.0 58.1 58.3 0.0 0.0 0.396990 15171 16260 1308-1310 1.000000 77.5 atpH-atpI_p3 atpH-atpI 3 GCGATACCCTCTACAGCTTG TCCCTGTCATGTTCCTTGGA 20 20 55.0 50.0 57.9 58.3 0.0 0.0 0.400507 15171 16260 1312-1314 1.000000 77.5 atpH-atpI_p4 atpH-atpI 4 CAGCAGCAATAACAGAAGCG TCCCTGTCATGTTCCTTGGA 20 20 50.0 50.0 57.8 58.3 0.0 0.0 0.428635 15171 16260 1233-1235 1.000000 77.3 atpH-atpI_p5 atpH-atpI 5 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 20 20 50.0 50.0 57.8 58.3 0.0 0.0 0.473359 15171 16260 1240-1242 1.000000 76.9 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CCTCATTTCCATCCCTGAGC AAAAGGATTTGCAGTCCCCC 20 20 55.0 50.0 58.0 58.4 0.0 14.6 0.377036 28514 29766 1305-1319 1.000000 78.6 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 CCTCATTTCCATCCCTGAGC GCGACACCCAGATTTGAACT 20 20 55.0 50.0 58.0 58.5 0.0 0.0 0.497788 28514 29766 1330-1344 1.000000 77.6 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 TCCCTCATTTCCATCCCTGA AAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 57.7 58.4 0.0 14.6 0.640766 28514 29766 1307-1321 1.000000 76.5 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 TCCCTCATTTCCATCCCTGA GCGACACCCAGATTTGAACT 20 20 50.0 50.0 57.7 58.5 0.0 0.0 0.761517 28514 29766 1332-1346 1.000000 75.5 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 CCTCATTTCCATCCCTGAGC TTTGTTGATCAGGCGACACC 20 20 55.0 50.0 58.0 58.8 0.0 0.0 0.786725 28514 29766 1342-1356 1.000000 75.3 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.3 58.1 0.0 0.0 0.395920 33999 35359 1463-1498 1.000000 79.7 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GGCGTAAGTCATCGGTTCAA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 58.3 58.2 0.0 0.0 0.528876 33999 35359 1453-1488 1.000000 78.6 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 ACTCAGGGGTAGAGTAACGC GGACCAGCCTACAAAAACGA 20 20 55.0 50.0 58.5 58.1 0.0 0.0 0.640926 33999 35359 1491-1526 1.000000 77.6 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GGCGTAAGTCATCGGTTCAA CAAAAACGAAACGGTCCCTC 20 20 50.0 50.0 58.3 57.6 0.0 0.0 0.677352 33999 35359 1452-1487 1.000000 77.4 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GGCGTAAGTCATCGGTTCAA AAGAGCAATAGACCGGACCA 20 20 50.0 50.0 58.3 58.4 0.0 9.5 0.714302 33999 35359 1477-1512 1.000000 77.1 psaA-pafII_p1 psaA-pafII 1 GCCCATTCCTCGAAAGAAGT CATTCCCTTGACCCGTACTG 20 20 50.0 55.0 57.9 58.0 4.1 0.0 0.139518 44412 45253 1003-1025 1.000000 81.6 psaA-pafII_p2 psaA-pafII 2 GCCCATTCCTCGAAAGAAGT ATTCCCTTGACCCGTACTGA 20 20 50.0 50.0 57.9 58.1 4.1 0.0 0.168358 44412 45253 1002-1024 1.000000 81.4 psaA-pafII_p3 psaA-pafII 3 GCCCATTCCTCGAAAGAAGT TTCCCTTGACCCGTACTGAT 20 20 50.0 50.0 57.9 58.1 4.1 0.0 0.168358 44412 45253 1001-1023 1.000000 81.4 psaA-pafII_p4 psaA-pafII 4 GCCCATTCCTCGAAAGAAGT CTTGCGCGTACCAATTGAAG 20 20 50.0 50.0 57.9 58.1 4.1 8.4 0.233145 44412 45253 928-950 1.000000 81.0 psaA-pafII_p5 psaA-pafII 5 GCCCATTCCTCGAAAGAAGT GAACAAAAAGCCGCCGAATT 20 20 50.0 45.0 57.9 58.2 4.1 0.0 0.337008 44412 45253 957-979 1.000000 80.1 accD-trnM-CAU_p1 accD-trnM-CAU 1 CCAATGGCTACGGGAATACC GTTGGGCGCTTTAACCATTC 20 20 55.0 50.0 58.1 58.0 0.0 7.6 0.116991 47332 48584 1371-1547 1.000000 83.2 accD-trnM-CAU_p2 accD-trnM-CAU 2 CCCAATGGCTACGGGAATAC GTTGGGCGCTTTAACCATTC 20 20 55.0 50.0 58.1 58.0 0.1 7.6 0.116991 47332 48584 1372-1548 1.000000 83.2 accD-trnM-CAU_p3 accD-trnM-CAU 3 TACCCGTTTGAACAGCATCC GTTGGGCGCTTTAACCATTC 20 20 50.0 50.0 58.2 58.0 0.0 7.6 0.193126 47332 48584 1337-1513 1.000000 82.7 accD-trnM-CAU_p4 accD-trnM-CAU 4 GGCTACGGGAATACCACCTA GTTGGGCGCTTTAACCATTC 20 20 55.0 50.0 58.3 58.0 0.0 7.6 0.300860 47332 48584 1366-1542 1.000000 81.8 accD-trnM-CAU_p5 accD-trnM-CAU 5 CATAACCCCAATGGCTACGG GTTGGGCGCTTTAACCATTC 20 20 55.0 50.0 58.4 58.0 0.0 7.6 0.404597 47332 48584 1378-1554 1.000000 80.9 trnM-CAU-trnS-GCU_p1 trnM-CAU-trnS-GCU 1 AGGTTCAATTCCTACTGGATGC CAATCCGACGCTTTAGTCCA 22 20 45.5 50.0 58.1 58.0 0.0 0.0 2.113534 48659 50334 1651-1947 1.000000 69.2 trnM-CAU-trnS-GCU_p2 trnM-CAU-trnS-GCU 2 AGGTTCAATTCCTACTGGATGC AATCCGACGCTTTAGTCCAC 22 20 45.5 50.0 58.1 58.0 0.0 0.0 2.114922 48659 50334 1650-1946 1.000000 69.2 trnM-CAU-trnS-GCU_p3 trnM-CAU-trnS-GCU 3 AGGTTCAATTCCTACTGGATGC ACGGAAAGAGAGGGATTCGA 22 20 45.5 50.0 58.1 58.2 0.0 0.0 2.260299 48659 50334 1705-2001 1.000000 67.9 trnM-CAU-trnS-GCU_p4 trnM-CAU-trnS-GCU 4 GGTTCAATTCCTACTGGATGC CAATCCGACGCTTTAGTCCA 21 20 47.6 50.0 56.7 58.0 0.0 0.0 2.343904 48659 50334 1650-1946 1.000000 67.3 trnM-CAU-trnS-GCU_p5 trnM-CAU-trnS-GCU 5 GGTTCAATTCCTACTGGATGC AATCCGACGCTTTAGTCCAC 21 20 47.6 50.0 56.7 58.0 0.0 0.0 2.345292 48659 50334 1649-1945 1.000000 67.3 trnS-GCU-rbcL_p1 trnS-GCU-rbcL 1 TGGACTAAAGCGTCGGATTG ACTACAGTTCGGTGGAGGAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.009953 50423 51517 1363-1406 1.000000 84.9 trnS-GCU-rbcL_p2 trnS-GCU-rbcL 2 GTGGACTAAAGCGTCGGATT ACTACAGTTCGGTGGAGGAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.011341 50423 51517 1364-1407 1.000000 84.9 trnS-GCU-rbcL_p3 trnS-GCU-rbcL 3 TGGACTAAAGCGTCGGATTG TCTTCACATTCACCGTGCAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.044166 50423 51517 1690-1733 1.000000 83.8 trnS-GCU-rbcL_p4 trnS-GCU-rbcL 4 TGGACTAAAGCGTCGGATTG TTGAATGCTACTGCGGGTAC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.023125 50423 51517 1842-1885 1.000000 83.7 trnS-GCU-rbcL_p5 trnS-GCU-rbcL 5 GTGGACTAAAGCGTCGGATT TTGAATGCTACTGCGGGTAC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.024512 50423 51517 1843-1886 1.000000 83.7 trnL-UAA-trnT-UGU_p1 trnL-UAA-trnT-UGU 1 ACCAATTTCGCCATATCCCC GCGATGCTCTAACCTCTGAG 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.137364 61236 61901 709-716 1.000000 81.1 trnL-UAA-trnT-UGU_p2 trnL-UAA-trnT-UGU 2 TAGCGTCTACCAATTTCGCC GCGATGCTCTAACCTCTGAG 20 20 50.0 55.0 58.1 57.9 0.0 0.0 0.202173 61236 61901 717-724 1.000000 80.6 trnL-UAA-trnT-UGU_p3 trnL-UAA-trnT-UGU 3 ACCAATTTCGCCATATCCCC GGAATCGAACCGATGACCAT 20 20 50.0 50.0 58.0 57.8 0.0 15.5 0.245117 61236 61901 741-748 1.000000 80.2 trnL-UAA-trnT-UGU_p4 trnL-UAA-trnT-UGU 4 TAGCGTCTACCAATTTCGCC GGAATCGAACCGATGACCAT 20 20 50.0 50.0 58.1 57.8 0.0 15.5 0.309926 61236 61901 749-756 1.000000 79.6 trnL-UAA-trnT-UGU_p5 trnL-UAA-trnT-UGU 5 AGTCCGTAGCGTCTACCAAT GCGATGCTCTAACCTCTGAG 20 20 50.0 55.0 58.2 57.9 10.2 0.0 0.372548 61236 61901 723-730 1.000000 79.2 pafI-cemA_p1 pafI-cemA 1 GGAAACAGGCTATAGCGCTT GAGAAATCCACCAGGGCAAA 20 20 50.0 50.0 58.0 58.1 18.7 0.0 0.121717 66196 67188 1146-1211 1.000000 83.1 pafI-cemA_p2 pafI-cemA 2 GGAAACAGGCTATAGCGCTT GGAATTCCTGAGCCCAAGAG 20 20 50.0 55.0 58.0 58.0 18.7 17.7 0.094961 66196 67188 1497-1562 1.000000 82.5 pafI-cemA_p3 pafI-cemA 3 CTAAAGTCTTGAGCAGCGGT GAGAAATCCACCAGGGCAAA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.162699 66196 67188 1624-1688 1.000000 81.6 pafI-cemA_p4 pafI-cemA 4 ACTAAAGTCTTGAGCAGCGG GGAATTCCTGAGCCCAAGAG 20 20 50.0 55.0 57.9 58.0 0.0 17.7 0.135943 66196 67188 1976-2040 1.000000 81.4 pafI-cemA_p5 pafI-cemA 5 CTAAAGTCTTGAGCAGCGGT GGAATTCCTGAGCCCAAGAG 20 20 50.0 55.0 57.9 58.0 0.0 17.7 0.135943 66196 67188 1975-2039 1.000000 81.4 petA-psbJ_p1 petA-psbJ 1 GAGAAGGTTCAATTGGCCGA ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.2 57.6 5.4 4.1 0.543756 69080 70169 1136-1153 1.000000 80.7 petA-psbJ_p2 petA-psbJ 2 AGAAGGTTCAATTGGCCGAA ATTCCGGATTGGGTTCATCC 20 20 45.0 50.0 57.7 57.6 5.4 4.1 0.651337 69080 70169 1135-1152 1.000000 79.8 petA-psbJ_p3 petA-psbJ 3 TGAGAAGGTTCAATTGGCCG ATTCCGGATTGGGTTCATCC 20 20 50.0 50.0 58.5 57.6 5.4 4.1 0.831347 69080 70169 1137-1154 1.000000 78.4 petA-psbJ_p4 petA-psbJ 4 GAGAAGGTTCAATTGGCCGA CTTGTGATCGGTTTAGTGGGT 20 21 50.0 47.6 58.2 57.9 5.4 0.0 1.232615 69080 70169 1176-1193 1.000000 75.0 petA-psbJ_p5 petA-psbJ 5 AGAAGGTTCAATTGGCCGAA CTTGTGATCGGTTTAGTGGGT 20 21 45.0 47.6 57.7 57.9 5.4 0.0 1.340196 69080 70169 1175-1192 1.000000 74.1 trnR-ACG-trnN-GUU_p1 trnR-ACG-trnN-GUU 1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 20 20 55.0 55.0 57.8 58.0 18.9 0.0 0.218238 114210 114783 708-715 1.000000 79.5 trnR-ACG-trnN-GUU_p2 trnR-ACG-trnN-GUU 2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 20 20 55.0 55.0 57.7 58.0 18.7 0.0 0.288158 114210 114783 745-752 1.000000 78.9 trnR-ACG-trnN-GUU_p3 trnR-ACG-trnN-GUU 3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 20 20 55.0 50.0 57.8 58.2 18.9 18.9 0.372539 114210 114783 659-666 1.000000 78.3 trnR-ACG-trnN-GUU_p4 trnR-ACG-trnN-GUU 4 GAACAGATCCAGTGGAGACG TGGTCGTAGGTTCGAATCCT 20 20 55.0 50.0 57.7 58.2 18.7 18.9 0.442458 114210 114783 696-703 1.000000 77.7 trnR-ACG-trnN-GUU_p5 trnR-ACG-trnN-GUU 5 CAGAGGATTAGAGCACGTGG CGATTGGTCGTAGGTTCGAA 20 20 55.0 50.0 57.8 57.7 18.9 0.0 0.483095 114210 114783 663-670 1.000000 77.5 ycf1-ndhF_p1 ycf1-ndhF 1 AAGATGGGGAAGATGGGGAA CGAATGGGGTTGGTGTTTTG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.147725 116876 116915 774-869 1.000000 84.2 ycf1-ndhF_p2 ycf1-ndhF 2 ATGGGGAAGATGGGGAAGAA CGAATGGGGTTGGTGTTTTG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.147725 116876 116915 771-866 1.000000 84.2 ycf1-ndhF_p3 ycf1-ndhF 3 TTGGGGAAGATGGGGAAGAT CGAATGGGGTTGGTGTTTTG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.147725 116876 116915 780-875 1.000000 84.2 ycf1-ndhF_p4 ycf1-ndhF 4 AAGATGGGGAAGATGGGGAA AATATGTAGGGGGTGGACGT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.185952 116876 116915 797-797 0.250000 48.5 ycf1-ndhF_p5 ycf1-ndhF 5 ATGGGGAAGATGGGGAAGAA AATATGTAGGGGGTGGACGT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.185952 116876 116915 794-794 0.250000 48.5 rps15-ycf1_p1 rps15-ycf1 1 AACACCATGCATTGATGGGT TGGATTTGCTTGTTCCGGAA 20 20 45.0 45.0 58.1 58.0 8.9 14.3 0.070329 129850 130220 1115-1136 1.000000 82.8 rps15-ycf1_p2 rps15-ycf1 2 AACACCATGCATTGATGGGT TTGGATTTGCTTGTTCCGGA 20 20 45.0 45.0 58.1 58.0 8.9 0.0 0.070329 129850 130220 1116-1137 1.000000 82.8 rps15-ycf1_p3 rps15-ycf1 3 TGCATTGATGGGTGATGAGG TGGATTTGCTTGTTCCGGAA 20 20 50.0 45.0 57.9 58.0 0.0 14.3 0.073216 129850 130220 1108-1129 1.000000 82.8 rps15-ycf1_p4 rps15-ycf1 4 TGCATTGATGGGTGATGAGG TTGGATTTGCTTGTTCCGGA 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.073216 129850 130220 1109-1130 1.000000 82.8 rps15-ycf1_p5 rps15-ycf1 5 GGTGATGAGGGCCCATATTG TGGATTTGCTTGTTCCGGAA 20 20 55.0 45.0 58.1 58.0 27.6 14.3 0.096566 129850 130220 1098-1119 1.000000 82.6 ycf1_p1 ycf1 1 TTCTCGATCGCTCTCATCCT AAGATGGGGAAGATGGGGAA 20 20 50.0 50.0 58.0 58.0 11.1 0.0 0.026025 134334 134483 1544-1670 1.000000 84.1 ycf1_p2 ycf1 2 TTCTCGATCGCTCTCATCCT ATGGGGAAGATGGGGAAGAA 20 20 50.0 50.0 58.0 58.0 11.1 0.0 0.026025 134334 134483 1541-1667 1.000000 84.1 ycf1_p3 ycf1 3 GTCCTTGAAGCAGGTTCCAA AAGATGGGGAAGATGGGGAA 20 20 50.0 50.0 58.0 58.0 9.9 0.0 0.019033 134334 134483 1278-1404 1.000000 82.7 ycf1_p4 ycf1 4 GTCCTTGAAGCAGGTTCCAA ATGGGGAAGATGGGGAAGAA 20 20 50.0 50.0 58.0 58.0 9.9 0.0 0.019033 134334 134483 1275-1401 1.000000 82.7 ycf1_p5 ycf1 5 GTCCTTGAAGCAGGTTCCAA TTGGGGAAGATGGGGAAGAT 20 20 50.0 50.0 58.0 58.0 9.9 0.0 0.019033 134334 134483 1284-1410 1.000000 82.6 trnN-GUU-trnR-ACG_p1 trnN-GUU-trnR-ACG 1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 20 20 55.0 55.0 58.0 57.8 0.0 18.9 0.218238 136567 137140 708-715 1.000000 79.5 trnN-GUU-trnR-ACG_p2 trnN-GUU-trnR-ACG 2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 20 20 55.0 55.0 58.0 57.7 0.0 18.7 0.288158 136567 137140 745-752 1.000000 78.9 trnN-GUU-trnR-ACG_p3 trnN-GUU-trnR-ACG 3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 58.2 57.8 18.9 18.9 0.372539 136567 137140 659-666 1.000000 78.3 trnN-GUU-trnR-ACG_p4 trnN-GUU-trnR-ACG 4 TGGTCGTAGGTTCGAATCCT GAACAGATCCAGTGGAGACG 20 20 50.0 55.0 58.2 57.7 18.9 18.7 0.442458 136567 137140 696-703 1.000000 77.7 trnN-GUU-trnR-ACG_p5 trnN-GUU-trnR-ACG 5 CGATTGGTCGTAGGTTCGAA CAGAGGATTAGAGCACGTGG 20 20 50.0 55.0 57.7 57.8 0.0 18.9 0.483095 136567 137140 663-670 1.000000 77.5