primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ccs1_p1	ccs1	1	TCCACTGCTCTACCAACTGA	AGAGCATATTTTAACTACACGACT	20	24	50.0	33.3	58.0	56.0	0.0	0.0	6.036226	3430	4737	1438-1438	1.000000	40.1
ccs1_p2	ccs1	2	TCCCGGCTTGTTTGCTTATT	AGAGCATATTTTAACTACACGACT	20	24	45.0	33.3	58.1	56.0	0.0	0.0	6.108673	3430	4737	1414-1414	1.000000	40.1
ccs1_p3	ccs1	3	ATCCCGGCTTGTTTGCTTAT	AGAGCATATTTTAACTACACGACT	20	24	45.0	33.3	57.9	56.0	0.0	0.0	6.170420	3430	4737	1415-1415	1.000000	40.1
ccs1_p4	ccs1	4	TCCACTGCTCTACCAACTGA	AGAGCATATTTTAACTACACGACTT	20	25	50.0	32.0	58.0	56.6	0.0	0.0	6.384724	3430	4737	1438-1438	1.000000	40.1
ccs1_p5	ccs1	5	CCACTGCTCTACCAACTGAG	AGAGCATATTTTAACTACACGACT	20	24	55.0	33.3	57.6	56.0	0.0	0.0	6.403353	3430	4737	1437-1437	1.000000	40.1
ycf55_p1	ycf55	1	AGGCCATAAATCACCACAGC	ACGTAAAGCCTATTTGAACCTCT	20	23	50.0	39.1	58.2	57.8	5.0	0.0	3.394185	5916	6635	800-800	1.000000	52.9
ycf55_p2	ycf55	2	GGCCATAAATCACCACAGCT	ACGTAAAGCCTATTTGAACCTCT	20	23	50.0	39.1	58.2	57.8	0.0	0.0	3.394185	5916	6635	799-799	1.000000	52.9
ycf55_p3	ycf55	3	AGGCCATAAATCACCACAGC	ACGTAAAGCCTATTTGAACCTC	20	22	50.0	40.9	58.2	56.4	5.0	0.0	3.787521	5916	6635	800-800	1.000000	49.8
ycf55_p4	ycf55	4	GGCCATAAATCACCACAGCT	ACGTAAAGCCTATTTGAACCTC	20	22	50.0	40.9	58.2	56.4	0.0	0.0	3.787521	5916	6635	799-799	1.000000	49.8
ycf55_p5	ycf55	5	GGCCATAAATCACCACAGCT	ACGTAAAGCCTATTTGAACCT	20	21	50.0	38.1	58.2	55.2	0.0	0.0	4.010915	5916	6635	799-799	1.000000	48.0
trg1_p1	trg1	1	ATTTCTTCCGAAGCACCGAT	TATGCAAAGACTCGAGACGG	20	20	45.0	50.0	57.6	57.4	3.0	21.7	0.964985	7884	8603	781-781	1.000000	72.4
trg1_p2	trg1	2	ACGTACCATTTTGCCCAGAT	TATGCAAAGACTCGAGACGG	20	20	45.0	50.0	57.5	57.4	0.0	21.7	1.069140	7884	8603	855-855	1.000000	71.5
trg1_p3	trg1	3	TCTTCCGAAGCACCGATAAG	TATGCAAAGACTCGAGACGG	20	20	50.0	50.0	57.4	57.4	3.0	21.7	1.140940	7884	8603	778-778	1.000000	71.0
trg1_p4	trg1	4	ATTTCTTCCGAAGCACCGAT	TTATGCAAAGACTCGAGACGG	20	21	45.0	47.6	57.6	58.1	3.0	21.7	1.527160	7884	8603	782-782	1.000000	67.9
trg1_p5	trg1	5	TTCTTCCGAAGCACCGATAA	TATGCAAAGACTCGAGACGG	20	20	45.0	50.0	57.0	57.4	3.0	21.7	1.604307	7884	8603	779-779	1.000000	67.3
rpoB_p1	rpoB	1	TACCACTGCTATACCCGGAG	TACCAACTAGAGGCCTCTGG	20	20	55.0	55.0	58.0	57.8	0.7	40.2	0.169600	10414	10533	228-228	1.000000	79.7
rpoB_p2	rpoB	2	ACGTCAATCTGTACCACTGC	TACCAACTAGAGGCCTCTGG	20	20	50.0	55.0	57.9	57.8	0.0	40.2	0.241007	10414	10533	239-239	1.000000	79.1
rpoB_p3	rpoB	3	CTTAGAACACGATGACGCGA	TACCAACTAGAGGCCTCTGG	20	20	50.0	55.0	58.1	57.8	15.9	40.2	0.269542	10414	10533	287-287	1.000000	78.7
rpoB_p4	rpoB	4	TCTTAGAACACGATGACGCG	TACCAACTAGAGGCCTCTGG	20	20	50.0	55.0	58.1	57.8	3.5	40.2	0.269542	10414	10533	288-288	1.000000	78.7
rpoB_p5	rpoB	5	TGGGCTCAAATATGCAACGT	TACCAACTAGAGGCCTCTGG	20	20	45.0	55.0	58.2	57.8	0.0	40.2	0.327837	10414	10533	255-255	1.000000	78.3
atpI_p1	atpI	1	GCACTATTCACGCCGAACTA	TGTGCGACTTGTGGAAAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.113986	19670	20371	1152-1152	1.000000	79.2
atpI_p2	atpI	2	GCACTATTCACGCCGAACTA	AACAGAAGCAGCTGAAACGA	20	20	50.0	45.0	58.1	58.0	0.0	20.4	0.129168	19670	20371	879-879	1.000000	79.1
atpI_p3	atpI	3	TGCTTATGACCCACGAGTTG	TGTGCGACTTGTGGAAAAGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124565	19670	20371	1810-1810	1.000000	79.0
atpI_p4	atpI	4	GCTTATGACCCACGAGTTGT	TGTGCGACTTGTGGAAAAGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.126021	19670	20371	1809-1809	1.000000	79.0
atpI_p5	atpI	5	GCACTATTCACGCCGAACTA	TAACGGATTGCGCATTAGCT	20	20	50.0	45.0	58.1	58.1	0.0	12.8	0.134623	19670	20371	1483-1483	1.000000	79.0
ccsA_p1	ccsA	1	CTTTGCTCCTGGTTGTAGCA	TGAACAGGAAGAAGTAATCTAGACT	20	25	50.0	36.0	58.1	57.0	3.9	0.0	6.125191	43440	44393	1116-1116	1.000000	40.1
ccsA_p2	ccsA	2	TCTTTGCTCCTGGTTGTAGC	TGAACAGGAAGAAGTAATCTAGACT	20	25	50.0	36.0	57.8	57.0	0.0	0.0	6.206415	43440	44393	1117-1117	1.000000	40.1
ccsA_p3	ccsA	3	CTTTGCTCCTGGTTGTAGCA	AGGAAGAAGTAATCTAGACTAGACCT	20	26	50.0	38.5	58.1	57.9	3.9	14.5	6.213340	43440	44393	1111-1111	1.000000	40.1
ccsA_p4	ccsA	4	TGGATCTTTGCTCCTGGTTG	TGAACAGGAAGAAGTAATCTAGACT	20	25	50.0	36.0	57.8	57.0	0.0	0.0	6.226433	43440	44393	1121-1121	1.000000	40.1
ccsA_p5	ccsA	5	GGATCTTTGCTCCTGGTTGT	TGAACAGGAAGAAGTAATCTAGACT	20	25	50.0	36.0	57.8	57.0	0.0	0.0	6.227987	43440	44393	1120-1120	1.000000	40.1
psbA_p1	psbA	1	AAAAACAGAAGGCTTAGAGC	CCTGGGAGGTTTTCAAGACC	20	20	40.0	55.0	53.8	58.1	0.0	0.0	4.287015	45077	46159	1186-1186	1.000000	46.0
psbA_p2	psbA	2	GCTTAGAGCCTTCTGTTTTT	CCTGGGAGGTTTTCAAGACC	20	20	40.0	55.0	53.8	58.1	0.0	0.0	4.287015	45077	46159	1175-1175	1.000000	46.0
psbA_p3	psbA	3	AAAAACAGAAGGCTTAGAGC	CTGGGAGGTTTTCAAGACCA	20	20	40.0	50.0	53.8	57.3	0.0	0.0	4.855020	45077	46159	1185-1185	1.000000	41.4
psbA_p4	psbA	4	GCTTAGAGCCTTCTGTTTTT	CTGGGAGGTTTTCAAGACCA	20	20	40.0	50.0	53.8	57.3	0.0	0.0	4.855020	45077	46159	1174-1174	1.000000	41.4
psbA_p5	psbA	5	GCTTAGAGCCTTCTGTTTTT	TCCTGGGAGGTTTTCAAGAC	20	20	40.0	50.0	53.8	57.0	0.0	0.0	5.149302	45077	46159	1176-1176	1.000000	40.3
psbA-rps6_p1	psbA-rps6	1	AAGTGATCCATGCGCAGAAT	CAGAGCTTCAACGAGACGAA	20	20	45.0	50.0	58.0	57.9	18.3	0.0	0.100323	46160	46605	602-603	1.000000	79.7
psbA-rps6_p2	psbA-rps6	2	AGTGATCCATGCGCAGAATT	CAGAGCTTCAACGAGACGAA	20	20	45.0	50.0	58.0	57.9	18.3	0.0	0.100323	46160	46605	601-602	1.000000	79.7
psbA-rps6_p3	psbA-rps6	3	GTGATCCATGCGCAGAATTG	CAGAGCTTCAACGAGACGAA	20	20	50.0	50.0	57.9	57.9	18.3	0.0	0.167227	46160	46605	600-601	1.000000	79.2
psbA-rps6_p4	psbA-rps6	4	CAGAATTGCTCCCAGAAGCT	CAGAGCTTCAACGAGACGAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.177652	46160	46605	588-589	1.000000	79.1
psbA-rps6_p5	psbA-rps6	5	ACAACCGAACCAACCAATGT	CAGAGCTTCAACGAGACGAA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.281584	46160	46605	640-641	1.000000	78.2
psbJ-trnE_p1	psbJ-trnE	1	TCCTCTTTGGATGGTGGCTA	AGGCCTAGGACATTTCCCTT	20	20	50.0	50.0	58.0	58.0	0.0	19.5	0.047506	50198	50371	332-332	1.000000	80.3
psbJ-trnE_p2	psbJ-trnE	2	CCTCTTTGGATGGTGGCTAC	AGGCCTAGGACATTTCCCTT	20	20	55.0	50.0	58.0	58.0	0.0	19.5	0.057043	50198	50371	331-331	1.000000	80.3
psbJ-trnE_p3	psbJ-trnE	3	CTTGCCGCCCTAGGTTTATT	AGGCCTAGGACATTTCCCTT	20	20	50.0	50.0	57.9	58.0	8.3	19.5	0.065703	50198	50371	301-301	1.000000	80.3
psbJ-trnE_p4	psbJ-trnE	4	ATTCCTCTTTGGATGGTGGC	AGGCCTAGGACATTTCCCTT	20	20	50.0	50.0	57.9	58.0	0.0	19.5	0.162893	50198	50371	334-334	1.000000	79.4
psbJ-trnE_p5	psbJ-trnE	5	TCCTCTTTGGATGGTGGCTA	TTACTTGCCCCCATCGTCTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.165873	50198	50371	353-353	1.000000	79.4
ycf80-trnM_p1	ycf80-trnM	1	CCGACAATTACAGGACAGGA	CAGCGTTGTCTCTGTGAGAA	20	20	50.0	50.0	56.9	57.9	0.0	0.0	1.190974	56820	57217	579-579	1.000000	70.7
ycf80-trnM_p2	ycf80-trnM	2	TCCGACAATTACAGGACAGG	CAGCGTTGTCTCTGTGAGAA	20	20	50.0	50.0	56.9	57.9	0.0	0.0	1.190974	56820	57217	580-580	1.000000	70.7
ycf80-trnM_p3	ycf80-trnM	3	CCGACAATTACAGGACAGGA	TATGCATCTGGCTGGATTCG	20	20	50.0	50.0	56.9	57.8	0.0	0.2	1.225273	56820	57217	602-602	1.000000	70.5
ycf80-trnM_p4	ycf80-trnM	4	TCCGACAATTACAGGACAGG	TATGCATCTGGCTGGATTCG	20	20	50.0	50.0	56.9	57.8	0.0	0.2	1.225273	56820	57217	603-603	1.000000	70.5
ycf80-trnM_p5	ycf80-trnM	5	CCGACAATTACAGGACAGGAA	CAGCGTTGTCTCTGTGAGAA	21	20	47.6	50.0	57.7	57.9	0.0	0.0	1.468446	56820	57217	579-579	1.000000	68.5
petD-petB_p1	petD-petB	1	CATGCAGGCTCACCGTAATA	TTTACCTGTGGTAGCAGCAG	20	20	50.0	50.0	57.8	57.5	0.0	14.0	0.711637	63121	63198	252-252	1.000000	74.9
petD-petB_p2	petD-petB	2	CATGCAGGCTCACCGTAATA	TACCTGTGGTAGCAGCAGTT	20	20	50.0	50.0	57.8	58.7	0.0	17.4	0.890878	63121	63198	250-250	1.000000	73.5
petD-petB_p3	petD-petB	3	CATGCAGGCTCACCGTAATA	TTACCTGTGGTAGCAGCAGT	20	20	50.0	50.0	57.8	58.7	0.0	17.4	0.890878	63121	63198	251-251	1.000000	73.5
petD-petB_p4	petD-petB	4	AGATCATTTGGCCATGCAGG	TTTACCTGTGGTAGCAGCAG	20	20	50.0	50.0	58.6	57.5	6.1	14.0	1.062691	63121	63198	264-264	1.000000	72.1
petD-petB_p5	petD-petB	5	CATGCAGGCTCACCGTAATA	TTTTACCTGTGGTAGCAGCA	20	20	50.0	45.0	57.8	57.1	0.0	0.0	1.179936	63121	63198	253-253	1.000000	71.2
ycf46-ycf24_p1	ycf46-ycf24	1	TGCGCAATACCTCATCAAGT	TGCATCTGAAGCAGATCGTC	20	20	45.0	50.0	57.6	58.1	8.2	3.1	0.469677	71654	71708	218-218	1.000000	77.0
ycf46-ycf24_p2	ycf46-ycf24	2	TGCGCAATACCTCATCAAGT	GCATCTGAAGCAGATCGTCT	20	20	45.0	50.0	57.6	57.8	8.2	6.9	0.623590	71654	71708	217-217	1.000000	75.7
ycf46-ycf24_p3	ycf46-ycf24	3	TGCGCAATACCTCATCAAGT	TTGCATCTGAAGCAGATCGT	20	20	45.0	45.0	57.6	57.6	8.2	7.7	0.793199	71654	71708	219-219	1.000000	74.4
ycf46-ycf24_p4	ycf46-ycf24	4	TTGCGCAATACCTCATCAAGT	TGCATCTGAAGCAGATCGTC	21	20	42.9	50.0	58.3	58.1	21.0	3.1	1.357717	71654	71708	219-219	1.000000	69.9
ycf46-ycf24_p5	ycf46-ycf24	5	TGCGCAATACCTCATCAAGT	GTTTGCATCTGAAGCAGATCG	20	21	45.0	47.6	57.6	58.0	8.2	7.7	1.450525	71654	71708	221-221	1.000000	69.1
tatC-groEL_p1	tatC-groEL	1	GCCATTTCATCCCCTTCAGT	TCGACACTTCAAAATGCAGC	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.836531	76380	76424	224-224	1.000000	73.7
tatC-groEL_p2	tatC-groEL	2	GCCATTTCATCCCCTTCAGT	CGACACTTCAAAATGCAGCT	20	20	50.0	45.0	57.9	57.3	0.0	0.0	0.843770	76380	76424	223-223	1.000000	73.6
tatC-groEL_p3	tatC-groEL	3	ATCCCCTTCAGTATCCAGCA	TCGACACTTCAAAATGCAGC	20	20	50.0	45.0	57.8	57.3	0.0	0.0	0.883300	76380	76424	216-216	1.000000	73.3
tatC-groEL_p4	tatC-groEL	4	TCCCCTTCAGTATCCAGCAT	TCGACACTTCAAAATGCAGC	20	20	50.0	45.0	57.8	57.3	0.0	0.0	0.883300	76380	76424	215-215	1.000000	73.3
tatC-groEL_p5	tatC-groEL	5	TCCCCTTCAGTATCCAGCAT	CGACACTTCAAAATGCAGCT	20	20	50.0	45.0	57.8	57.3	0.0	0.0	0.890540	76380	76424	214-214	1.000000	73.2
groEL_p1	groEL	1	GCGGGTGCTTGAAAAACTTT	GACTCATAAGCCCAAGGTCG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.124086	76425	78029	1991-1991	1.000000	79.3
groEL_p2	groEL	2	ATTCTCCGGGTGCAAATTGT	GACTCATAAGCCCAAGGTCG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.135319	76425	78029	2029-2029	1.000000	79.2
groEL_p3	groEL	3	GCGGGTGCTTGAAAAACTTT	GATTGGGTCAACTGGGACTC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.180754	76425	78029	2414-2414	1.000000	78.8
groEL_p4	groEL	4	ATTCTCCGGGTGCAAATTGT	GATTGGGTCAACTGGGACTC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.191987	76425	78029	2452-2452	1.000000	78.7
groEL_p5	groEL	5	ATTGCGGGTGCTTGAAAAAC	GACTCATAAGCCCAAGGTCG	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.194110	76425	78029	1994-1994	1.000000	78.7
psbB_p1	psbB	1	AGTCCCTATTAGCAGAAAAGTGT	AAACAGCGTAAGAAAGTCAA	23	20	39.1	35.0	57.2	53.4	0.0	0.0	8.369974	85109	86638	1660-1660	1.000000	40.1
psbB_p2	psbB	2	AGTCCCTATTAGCAGAAAAGTGT	AACAGCGTAAGAAAGTCAAA	23	20	39.1	35.0	57.2	53.4	0.0	0.0	8.369974	85109	86638	1659-1659	1.000000	40.1
psbB_p3	psbB	3	AGTCCCTATTAGCAGAAAAGTGT	ACAGCGTAAGAAAGTCAAAA	23	20	39.1	35.0	57.2	53.4	0.0	0.0	8.369974	85109	86638	1658-1658	1.000000	40.1
psbB_p4	psbB	4	AGTCCCTATTAGCAGAAAAGTGT	AACAGCGTAAGAAAGTCAAAA	23	21	39.1	33.3	57.2	54.3	0.0	0.0	8.484794	85109	86638	1659-1659	1.000000	40.1
psbB_p5	psbB	5	AGTCCCTATTAGCAGAAAAGTGT	ACAGCGTAAGAAAGTCAAAAA	23	21	39.1	33.3	57.2	54.3	0.0	0.0	8.484794	85109	86638	1658-1658	1.000000	40.1
trnW-trnS_p1	trnW-trnS	1	AGTTCGCGCTCTTAGTTCAG	ACCCGTGATAGGAGAGGATG	20	20	50.0	55.0	58.0	58.0	5.6	0.0	0.018533	92853	92911	234-234	1.000000	80.2
trnW-trnS_p2	trnW-trnS	2	GGGATAGTTCGCGCTCTTAG	ACCCGTGATAGGAGAGGATG	20	20	55.0	55.0	57.9	58.0	5.6	0.0	0.055788	92853	92911	239-239	1.000000	79.9
trnW-trnS_p3	trnW-trnS	3	GCGCTCTTAGTTCAGTTGGT	ACCCGTGATAGGAGAGGATG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.205481	92853	92911	229-229	1.000000	78.7
trnW-trnS_p4	trnW-trnS	4	GTTCGCGCTCTTAGTTCAGT	ACCCGTGATAGGAGAGGATG	20	20	50.0	55.0	58.3	58.0	5.6	0.0	0.303162	92853	92911	233-233	1.000000	77.9
trnW-trnS_p5	trnW-trnS	5	TCGCGCTCTTAGTTCAGTTG	ACCCGTGATAGGAGAGGATG	20	20	50.0	55.0	58.3	58.0	5.6	0.0	0.304340	92853	92911	231-231	1.000000	77.9