primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
groEL-ycf65_p1	groEL-ycf65	1	TGCGACTAAAGCAGCTGTAG	TCCCCAAACGAATGAAAGCA	20	20	50.0	45.0	58.0	58.0	17.1	0.0	0.015415	1588	2625	1868-1868	0.500000	59.9
groEL-ycf65_p2	groEL-ycf65	2	CGCGTTCTGGACTTCAAAAC	TCCCCAAACGAATGAAAGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030424	1588	2625	1566-1566	0.500000	59.8
groEL-ycf65_p3	groEL-ycf65	3	CGTTCTGGACTTCAAAACGC	TCCCCAAACGAATGAAAGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030424	1588	2625	1564-1564	0.500000	59.8
groEL-ycf65_p4	groEL-ycf65	4	GCGTTCTGGACTTCAAAACG	TCCCCAAACGAATGAAAGCA	20	20	50.0	45.0	58.0	58.0	0.9	0.0	0.030424	1588	2625	1565-1565	0.500000	59.8
groEL-ycf65_p5	groEL-ycf65	5	GGACTTCAAAACGCAGCTTC	TCCCCAAACGAATGAAAGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.049647	1588	2625	1558-1558	0.500000	59.6
trnA-UGC-rnl_p1	trnA-UGC-rnl	1	GGGGGTATAGCTCAGTTGGT	TCATCGCTTCTGAATGCCAA	20	20	55.0	45.0	58.5	57.9	3.8	0.0	0.604124	9501	9572	197-200	1.000000	80.0
trnA-UGC-rnl_p2	trnA-UGC-rnl	2	GGGGGTATAGCTCAGTTGGT	TTCATCGCTTCTGAATGCCA	20	20	55.0	45.0	58.5	57.9	3.8	0.0	0.604124	9501	9572	198-201	1.000000	80.0
trnA-UGC-rnl_p3	trnA-UGC-rnl	3	GGGGGTATAGCTCAGTTGGT	CGCTTCTGAATGCCAAGGTA	20	20	55.0	50.0	58.5	58.3	3.8	0.0	0.753906	9501	9572	193-196	1.000000	78.9
trnA-UGC-rnl_p4	trnA-UGC-rnl	4	GGGGGTATAGCTCAGTTGGT	GCCCTTCATCGCTTCTGAAT	20	20	55.0	50.0	58.5	58.3	3.8	0.0	0.818576	9501	9572	202-205	1.000000	78.2
trnA-UGC-rnl_p5	trnA-UGC-rnl	5	GGGGGTATAGCTCAGTTGGT	AATGCTTACTTCCAGCTCCC	20	20	55.0	50.0	58.5	57.6	3.8	0.0	0.918587	9501	9572	245-248	1.000000	76.5
ccsA_p1	ccsA	1	AAGCGGAAACTGCTACACAA	AGGCTGGACAAATTGCTTCA	20	20	45.0	45.0	58.0	58.0	5.5	0.0	0.038558	16087	17064	2163-2163	0.500000	59.7
ccsA_p2	ccsA	2	AGCGGAAACTGCTACACAAA	AGGCTGGACAAATTGCTTCA	20	20	45.0	45.0	58.0	58.0	3.1	0.0	0.038558	16087	17064	2162-2162	0.500000	59.7
ccsA_p3	ccsA	3	AAATCGTCGTATGGCTGCAT	AGGCTGGACAAATTGCTTCA	20	20	45.0	45.0	58.0	58.0	0.5	0.0	0.046793	16087	17064	2145-2145	0.500000	59.6
ccsA_p4	ccsA	4	AAGCGGAAACTGCTACACAA	TGAATGACATAGCGTCCTGC	20	20	45.0	50.0	58.0	58.1	5.5	0.0	0.098232	16087	17064	1788-1788	0.500000	59.2
ccsA_p5	ccsA	5	AGCGGAAACTGCTACACAAA	TGAATGACATAGCGTCCTGC	20	20	45.0	50.0	58.0	58.1	3.1	0.0	0.098232	16087	17064	1787-1787	0.500000	59.2
minE-rpl32_p1	minE-rpl32	1	TTGTATTAGCCCATGACCGC	CGTCTAGCAAACTGGAGCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.242439	27195	28101	1248-1248	0.500000	58.1
minE-rpl32_p2	minE-rpl32	2	GCTTGTCGATTAGATGGGCA	CGTCTAGCAAACTGGAGCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.250674	27195	28101	1464-1464	0.500000	58.0
minE-rpl32_p3	minE-rpl32	3	CCGCTCTACGTTAAATGCCT	CGTCTAGCAAACTGGAGCAA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.268300	27195	28101	1232-1232	0.500000	57.9
minE-rpl32_p4	minE-rpl32	4	TAAAACGCTTGCGCAGTTTT	CGTCTAGCAAACTGGAGCAA	20	20	40.0	50.0	57.8	58.2	29.7	0.0	0.400527	27195	28101	1400-1400	0.500000	56.8
minE-rpl32_p5	minE-rpl32	5	TTAAAACGCTTGCGCAGTTT	CGTCTAGCAAACTGGAGCAA	20	20	40.0	50.0	57.8	58.2	17.6	0.0	0.400527	27195	28101	1401-1401	0.500000	56.8
rbcR-rpl27_p1	rbcR-rpl27	1	TACTCGACGCATTTCTTGGG	GGGAATGTAAGTGTAGGGCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.055117	29769	30846	2101-2101	0.500000	59.6
rbcR-rpl27_p2	rbcR-rpl27	2	TACTCGACGCATTTCTTGGG	TGCGTCCGAAGAAAAAGACA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.061905	29769	30846	2004-2004	0.500000	59.5
rbcR-rpl27_p3	rbcR-rpl27	3	TACTCGACGCATTTCTTGGG	CGGCGATGATACTGAAGGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.062086	29769	30846	2344-2344	0.500000	59.5
rbcR-rpl27_p4	rbcR-rpl27	4	TACTCGACGCATTTCTTGGG	AGGAGGGATCCTTTGGGAAA	20	20	50.0	50.0	58.0	57.9	0.0	12.6	0.079505	29769	30846	2329-2329	0.500000	59.4
rbcR-rpl27_p5	rbcR-rpl27	5	GGTCTTCTTCATTGGGCCAA	GGGAATGTAAGTGTAGGGCG	20	20	50.0	55.0	58.1	58.1	23.3	0.0	0.127179	29769	30846	1713-1713	0.500000	59.0
rnl-trnA-UGC_p1	rnl-trnA-UGC	1	TCATCGCTTCTGAATGCCAA	GGGGGTATAGCTCAGTTGGT	20	20	45.0	55.0	57.9	58.5	0.0	3.8	0.604124	34567	34638	197-200	1.000000	80.0
rnl-trnA-UGC_p2	rnl-trnA-UGC	2	TTCATCGCTTCTGAATGCCA	GGGGGTATAGCTCAGTTGGT	20	20	45.0	55.0	57.9	58.5	0.0	3.8	0.604124	34567	34638	198-201	1.000000	80.0
rnl-trnA-UGC_p3	rnl-trnA-UGC	3	CGCTTCTGAATGCCAAGGTA	GGGGGTATAGCTCAGTTGGT	20	20	50.0	55.0	58.3	58.5	0.0	3.8	0.753906	34567	34638	193-196	1.000000	78.9
rnl-trnA-UGC_p4	rnl-trnA-UGC	4	GCCCTTCATCGCTTCTGAAT	GGGGGTATAGCTCAGTTGGT	20	20	50.0	55.0	58.3	58.5	0.0	3.8	0.818576	34567	34638	202-205	1.000000	78.2
rnl-trnA-UGC_p5	rnl-trnA-UGC	5	AATGCTTACTTCCAGCTCCC	GGGGGTATAGCTCAGTTGGT	20	20	50.0	55.0	57.6	58.5	0.0	3.8	0.918587	34567	34638	245-248	1.000000	76.5
ycf26_p1	ycf26	1	CTCATGCGGCTTATGAAGGT	CAAGTCCCTTCACCAGCTTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054076	38583	39281	1460-1460	0.500000	59.6
ycf26_p2	ycf26	2	TTTGAACCAGTGACCACCTG	CAAGTCCCTTCACCAGCTTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.064452	38583	39281	1080-1080	0.500000	59.5
ycf26_p3	ycf26	3	TTGTTACTAACCGAGCTGCC	CAAGTCCCTTCACCAGCTTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.102067	38583	39281	1484-1484	0.500000	59.2
ycf26_p4	ycf26	4	AAGGTCTCTGTCCTGATCCC	CAAGTCCCTTCACCAGCTTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.140345	38583	39281	1190-1190	0.500000	58.9
ycf26_p5	ycf26	5	GGTCTCTGTCCTGATCCCTT	CAAGTCCCTTCACCAGCTTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.140345	38583	39281	1188-1188	0.500000	58.9
rps4-rbcL_p1	rps4-rbcL	1	CCTACTATTCCAGCCGCAAG	GCAGTAGAAGACTCACCTGC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.132116	40315	42067	2259-2259	0.500000	58.9
rps4-rbcL_p2	rps4-rbcL	2	ATGCAAAGCGAATCAAGGGA	GCAGTAGAAGACTCACCTGC	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.165084	40315	42067	2351-2351	0.500000	58.7
rps4-rbcL_p3	rps4-rbcL	3	TGCAAAGCGAATCAAGGGAT	GCAGTAGAAGACTCACCTGC	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.165084	40315	42067	2350-2350	0.500000	58.7
rps4-rbcL_p4	rps4-rbcL	4	CCTACTATTCCAGCCGCAAG	ACAACTGTCCATGTTGCAGT	20	20	55.0	45.0	58.1	57.9	0.0	6.5	0.186057	40315	42067	2274-2274	0.500000	58.5
rps4-rbcL_p5	rps4-rbcL	5	GGAATGGCGCCTACTATTCC	GCAGTAGAAGACTCACCTGC	20	20	55.0	55.0	58.2	58.0	29.9	0.0	0.193512	40315	42067	2268-2268	0.500000	58.5
rne_p1	rne	1	TTATGAGTCCGTTGCCTTCG	AACAAACCCGAATCTTGGCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.009206	46652	47965	2333-2333	0.500000	59.9
rne_p2	rne	2	TTATGAGTCCGTTGCCTTCG	ACAAACCCGAATCTTGGCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.009206	46652	47965	2332-2332	0.500000	59.9
rne_p3	rne	3	TTATGAGTCCGTTGCCTTCG	TCTGAAAAATGGGGTGGTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.012825	46652	47965	2095-2095	0.500000	59.9
rne_p4	rne	4	CTCGCCTTTCAGAATCGGAA	AACAAACCCGAATCTTGGCT	20	20	50.0	45.0	58.0	58.0	3.7	0.0	0.014769	46652	47965	1825-1825	0.500000	59.9
rne_p5	rne	5	CTCGCCTTTCAGAATCGGAA	ACAAACCCGAATCTTGGCTT	20	20	50.0	45.0	58.0	58.0	3.7	0.0	0.014769	46652	47965	1824-1824	0.500000	59.9
trnK-UUU-ycf12_p1	trnK-UUU-ycf12	1	AAGGTAAGTACAGCCCCCAT	TATGGAGCTCCTCTATGCCC	20	20	50.0	55.0	58.0	58.1	0.0	32.3	0.074563	51234	51787	1248-1248	0.500000	59.4
trnK-UUU-ycf12_p2	trnK-UUU-ycf12	2	AAGGTAAGTACAGCCCCCAT	TAGCGCGACACAAAGATGAA	20	20	50.0	45.0	58.0	57.9	0.0	12.1	0.091857	51234	51787	1268-1268	0.500000	59.3
trnK-UUU-ycf12_p3	trnK-UUU-ycf12	3	AAGGTAAGTACAGCCCCCAT	TTAGCGCGACACAAAGATGA	20	20	50.0	45.0	58.0	57.9	0.0	12.1	0.091857	51234	51787	1269-1269	0.500000	59.3
trnK-UUU-ycf12_p4	trnK-UUU-ycf12	4	TCACGGAGGTAACAGGGATT	TATGGAGCTCCTCTATGCCC	20	20	50.0	55.0	58.1	58.1	0.0	32.3	0.108838	51234	51787	1201-1201	0.500000	59.1
trnK-UUU-ycf12_p5	trnK-UUU-ycf12	5	TTCACGGAGGTAACAGGGAT	TATGGAGCTCCTCTATGCCC	20	20	50.0	55.0	58.1	58.1	0.0	32.3	0.108838	51234	51787	1202-1202	0.500000	59.1
rps2_p1	rps2	1	TGGACAAGCAGCTATTTGCA	AGAAGATGGGGGAGACACAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.136449	66242	66940	1755-1755	0.500000	58.9
rps2_p2	rps2	2	TGGACAAGCAGCTATTTGCA	GAAGATGGGGGAGACACAAC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.206999	66242	66940	1754-1754	0.500000	58.3
rps2_p3	rps2	3	TGGACAAGCAGCTATTTGCA	CGAAGGAAAAGCTGACTGGT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.207421	66242	66940	1542-1542	0.500000	58.3
rps2_p4	rps2	4	TGGACAAGCAGCTATTTGCA	AAAGCTGACTGGTTACGTGG	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.212703	66242	66940	1535-1535	0.500000	58.3
rps2_p5	rps2	5	TGGACAAGCAGCTATTTGCA	CATTGCACGGAATTATGGGC	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.230252	66242	66940	1335-1335	0.500000	58.2
infB_p1	infB	1	TATCGTCGCAAAGCTTCTCC	TTTCTTGTTTACTCGCGGGT	20	20	50.0	45.0	58.1	57.8	0.0	19.2	0.321032	77835	80177	3000-3000	0.500000	57.4
infB_p2	infB	2	TATCGTCGCAAAGCTTCTCC	TCTTGTTTACTCGCGGGTAA	20	20	50.0	45.0	58.1	56.9	0.0	27.0	1.182413	77835	80177	2998-2998	0.500000	50.5
infB_p3	infB	3	TATCGTCGCAAAGCTTCTCC	TTCTTGTTTACTCGCGGGTA	20	20	50.0	45.0	58.1	56.9	0.0	16.4	1.182413	77835	80177	2999-2999	0.500000	50.5
infB_p4	infB	4	CGTCGCAAAGCTTCTCCAAT	TTTCTTGTTTACTCGCGGGT	20	20	50.0	45.0	59.2	57.8	0.0	19.2	1.444826	77835	80177	2997-2997	0.500000	48.4
infB_p5	infB	5	TATCGTCGCAAAGCTTCTCC	TTCTTGTTTACTCGCGGGTAA	20	21	50.0	42.9	58.1	57.6	0.0	27.0	1.461049	77835	80177	2999-2999	0.500000	48.3
ycf36_p1	ycf36	1	CAAGGGATGGGTGGACAAAA	CTCGTAGCCATCCTCCTGTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.025582	88315	88809	973-973	0.500000	59.8
ycf36_p2	ycf36	2	CAAGGGATGGGTGGACAAAA	ATGCATTAGACCAGCTCTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043690	88315	88809	1410-1410	0.500000	59.7
ycf36_p3	ycf36	3	GTGCTGCAATCGTTTGTACC	CTCGTAGCCATCCTCCTGTA	20	20	50.0	55.0	58.0	58.0	2.8	0.0	0.053593	88315	88809	1221-1221	0.500000	59.6
ycf36_p4	ycf36	4	CAAGGGATGGGTGGACAAAA	CATTGGCCTTCGGGTTATGA	20	20	50.0	50.0	58.0	57.9	0.0	3.3	0.059577	88315	88809	807-807	0.500000	59.5
ycf36_p5	ycf36	5	CAAGGGATGGGTGGACAAAA	AAGCATTGTTCTTTGCGAGC	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.064337	88315	88809	1109-1109	0.500000	59.5
rps3_p1	rps3	1	ATCTGTGAGCACTACCCTGT	CCGCTCTTGCTGTAATGGAT	20	20	50.0	50.0	58.1	58.0	2.0	0.0	0.096781	102986	103645	1036-1036	0.500000	59.2
rps3_p2	rps3	2	TGAGCACTACCCTGTAGCTT	CCGCTCTTGCTGTAATGGAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.102374	102986	103645	1031-1031	0.500000	59.2
rps3_p3	rps3	3	ATCTGTGAGCACTACCCTGT	TCCAGAGCTTGTAACCCGTA	20	20	50.0	50.0	58.1	58.1	2.0	0.0	0.128336	102986	103645	914-914	0.500000	59.0
rps3_p4	rps3	4	TGAGCACTACCCTGTAGCTT	TCCAGAGCTTGTAACCCGTA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.133928	102986	103645	909-909	0.500000	58.9
rps3_p5	rps3	5	ATCTGTGAGCACTACCCTGT	AACTACACGATTTCCACGGG	20	20	50.0	50.0	58.1	57.9	2.0	0.0	0.144772	102986	103645	885-885	0.500000	58.8
psbN-psbN_p1	psbN-psbN	1	AGCCCTAGAGGATGGTACAC	TGTACACAGCTTTTGGTCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.113941	117662	119925	2758-2758	0.500000	59.1
psbN-psbN_p2	psbN-psbN	2	GGAGGAAAACTAAACGGGCA	TGTACACAGCTTTTGGTCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.124095	117662	119925	2887-2887	0.500000	59.0
psbN-psbN_p3	psbN-psbN	3	GCAGAAGTGACTGAGCAAGT	TGTACACAGCTTTTGGTCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.148018	117662	119925	2644-2644	0.500000	58.8
psbN-psbN_p4	psbN-psbN	4	AGCCCTAGAGGATGGTACAC	TAGGTCCCTTCCGCTTTACA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.148223	117662	119925	2901-2901	0.500000	58.8
psbN-psbN_p5	psbN-psbN	5	TGCAGAAGTGACTGAGCAAG	TGTACACAGCTTTTGGTCCC	20	20	50.0	50.0	58.1	58.0	4.7	0.0	0.149331	117662	119925	2645-2645	0.500000	58.8
psbN-psbH_p1	psbN-psbH	1	GGGACCAAAAGCTGTGTACA	GCTGCCTAAACTAGCCCAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.082933	120050	121056	1295-1295	0.500000	59.3
psbN-psbH_p2	psbN-psbH	2	ATTACCCCTCATCCTACCCG	GCTGCCTAAACTAGCCCAAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.084229	120050	121056	1885-1885	0.500000	59.3
psbN-psbH_p3	psbN-psbH	3	TCAGATCTTTCGATCCGGGA	GCTGCCTAAACTAGCCCAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.125958	120050	121056	1311-1311	0.500000	59.0
psbN-psbH_p4	psbN-psbH	4	GGGACCAAAAGCTGTGTACA	TAGCTGCCTAAACTAGCCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.150211	120050	121056	1297-1297	0.500000	58.8
psbN-psbH_p5	psbN-psbH	5	ATTACCCCTCATCCTACCCG	TAGCTGCCTAAACTAGCCCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.151506	120050	121056	1887-1887	0.500000	58.8
dnaB_p1	dnaB	1	TCAGTTGGTAGAGCAGTGGA	ACTCCCGCTGCTTTAGAAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.110286	130136	131941	2611-2611	0.500000	59.1
dnaB_p2	dnaB	2	TCAGTTGGTAGAGCAGTGGA	CTCCCGCTGCTTTAGAAAGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.110286	130136	131941	2610-2610	0.500000	59.1
dnaB_p3	dnaB	3	GAGGGAGATACAGCGGTAGT	ACTCCCGCTGCTTTAGAAAG	20	20	55.0	50.0	58.0	57.9	2.5	0.0	0.116539	130136	131941	2858-2858	0.500000	59.1
dnaB_p4	dnaB	4	GAGGGAGATACAGCGGTAGT	CTCCCGCTGCTTTAGAAAGT	20	20	55.0	50.0	58.0	57.9	2.5	0.0	0.116539	130136	131941	2857-2857	0.500000	59.1
dnaB_p5	dnaB	5	TCAGTTGGTAGAGCAGTGGA	GCCTTAAACCACTCGACCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119106	130136	131941	2045-2045	0.500000	59.0
ycf29_p1	ycf29	1	AGAGTGGCTTGACCTACACT	TTCTAAATCAGGCGGACGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021487	135447	136139	1451-1451	0.500000	59.8
ycf29_p2	ycf29	2	AGAGTGGCTTGACCTACACT	TTGACTCCGTTATTCGCAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021487	135447	136139	2081-2081	0.500000	59.8
ycf29_p3	ycf29	3	AGAGTGGCTTGACCTACACT	ACTCCGTTATTCGCAGGTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022875	135447	136139	2078-2078	0.500000	59.8
ycf29_p4	ycf29	4	AGAGTGGCTTGACCTACACT	GACTCCGTTATTCGCAGGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022875	135447	136139	2079-2079	0.500000	59.8
ycf29_p5	ycf29	5	AGAGTGGCTTGACCTACACT	AAAGTCCATGCCACAATGGT	20	20	50.0	45.0	58.0	58.0	0.0	3.3	0.030914	135447	136139	2022-2022	0.500000	59.8