primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK_p1	matK	1	TGCTAGGTCTCATAACCCAA	GACTGTATCGCACTATGTGTCA	20	22	45.0	45.5	55.5	58.0	0.0	0.0	4.491792	1978	3492	1612-1612	1.000000	44.1
matK_p2	matK	2	TGCTAGGTCTCATAACCCAA	TGACTGTATCGCACTATGTGTC	20	22	45.0	45.5	55.5	58.0	0.0	0.0	4.491792	1978	3492	1613-1613	1.000000	44.1
matK_p3	matK	3	TGCTAGGTCTCATAACCCAA	ACCTCATTTTGACTGTATCGCA	20	22	45.0	40.9	55.5	58.1	0.0	0.0	4.525125	1978	3492	1622-1622	1.000000	43.8
matK_p4	matK	4	GGTATGCTAGGTCTCATAACCC	GACTGTATCGCACTATGTGTCA	22	22	50.0	45.5	57.5	58.0	0.0	0.0	4.567598	1978	3492	1616-1616	1.000000	43.5
matK_p5	matK	5	GGTATGCTAGGTCTCATAACCC	TGACTGTATCGCACTATGTGTC	22	22	50.0	45.5	57.5	58.0	0.0	0.0	4.567598	1978	3492	1617-1617	1.000000	43.5
trnK-AAA-rps16_p1	trnK-AAA-rps16	1	AAAGCCGAGTACTCTACCGT	AAAGGCGGGGATTTTTACGT	20	20	50.0	45.0	58.2	58.1	20.3	0.0	0.254642	4260	4946	752-773	1.000000	80.2
trnK-AAA-rps16_p2	trnK-AAA-rps16	2	AAGCCGAGTACTCTACCGTT	AAAGGCGGGGATTTTTACGT	20	20	50.0	45.0	58.2	58.1	20.3	0.0	0.254642	4260	4946	751-772	1.000000	80.2
trnK-AAA-rps16_p3	trnK-AAA-rps16	3	TCTACCGCATATCTGGACGA	AAAGGCGGGGATTTTTACGT	20	20	50.0	45.0	57.7	58.1	0.0	0.0	0.351811	4260	4946	817-838	1.000000	79.2
trnK-AAA-rps16_p4	trnK-AAA-rps16	4	CCGCATATCTGGACGAATCC	AAAGGCGGGGATTTTTACGT	20	20	55.0	45.0	58.3	58.1	0.0	0.0	0.371428	4260	4946	813-834	1.000000	79.1
trnK-AAA-rps16_p5	trnK-AAA-rps16	5	ACCGCATATCTGGACGAATC	AAAGGCGGGGATTTTTACGT	20	20	50.0	45.0	57.6	58.1	0.0	0.0	0.526761	4260	4946	814-835	1.000000	77.8
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	CCACTCAGCCATCTCTCCTA	TGAATCAAACCGAGAGACCC	20	20	55.0	50.0	57.9	57.2	0.0	0.0	0.841705	7629	8245	720-738	1.000000	73.5
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.8	57.2	0.0	0.0	0.926353	7629	8245	733-751	1.000000	72.8
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	58.2	57.2	2.0	0.0	0.965511	7629	8245	731-749	1.000000	72.5
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	CCACTCAGCCATCTCTCCTA	GAATCAAACCGAGAGACCCT	20	20	55.0	50.0	57.9	56.9	0.0	0.0	1.140559	7629	8245	719-737	1.000000	71.1
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	20	20	50.0	50.0	57.6	57.2	0.9	0.0	1.172484	7629	8245	728-746	1.000000	70.8
rpoB-trnC-UGC_p1	rpoB-trnC-UGC	1	GTTCCCTCTTTTCCATCCCC	AGGGGATTTCAAGCCTGTTG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.216529	25853	26928	1196-1197	1.000000	78.3
rpoB-trnC-UGC_p2	rpoB-trnC-UGC	2	GTTCCCTCTTTTCCATCCCC	GGGGATTTCAAGCCTGTTGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.512808	25853	26928	1195-1196	1.000000	76.0
rpoB-trnC-UGC_p3	rpoB-trnC-UGC	3	GTTCCCTCTTTTCCATCCCC	GTAGGGGATTTCAAGCCTGT	20	20	55.0	50.0	57.9	57.2	0.0	0.0	0.954303	25853	26928	1198-1199	1.000000	72.4
rpoB-trnC-UGC_p4	rpoB-trnC-UGC	4	TGTTCCCTCTTTTCCATCCC	AGGGGATTTCAAGCCTGTTG	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.970435	25853	26928	1197-1198	1.000000	72.3
rpoB-trnC-UGC_p5	rpoB-trnC-UGC	5	TCTTTTCCATCCCCGAGCAT	AGGGGATTTCAAGCCTGTTG	20	20	50.0	50.0	59.1	58.1	0.0	0.0	1.152149	25853	26928	1190-1191	1.000000	70.9
rbcL-psaI_p1	rbcL-psaI	1	GCAATTAAACCCGGCTCAAC	CTTGATAGCCATTCCCACCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.022318	56148	56267	2361-2368	1.000000	84.5
rbcL-psaI_p2	rbcL-psaI	2	GCAATTAAACCCGGCTCAAC	TAGCCATTCCCAATGTTCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.060535	56148	56267	2443-2450	1.000000	84.0
rbcL-psaI_p3	rbcL-psaI	3	GCAATTAAACCCGGCTCAAC	TTTCCGACGAATCCGAATCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.078633	56148	56267	2483-2490	1.000000	83.8
rbcL-psaI_p4	rbcL-psaI	4	ACCTCGACCTCTTTACGGAT	TCCGGCTAGAGGTAGTTACC	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.495848	56148	56267	342-386	1.000000	82.2
rbcL-psaI_p5	rbcL-psaI	5	GCAATTAAACCCGGCTCAAC	GCTGTTCAAATTCGGCTTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026589	56148	56267	2393-2393	0.500000	59.8
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCCTGTGATCGG	20	20	45.0	55.0	57.6	58.1	19.2	0.0	0.542229	63345	64182	1025-1025	1.000000	75.7
petA-psbJ_p2	petA-psbJ	2	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCCTGTGATCGG	20	20	45.0	55.0	57.4	58.1	0.0	0.0	0.699335	63345	64182	999-999	1.000000	74.5
petA-psbJ_p3	petA-psbJ	3	TCCATTACGTGTCCAAGGAC	GCTGGTATTCCTGTGATCGG	20	20	50.0	55.0	57.2	58.1	12.3	0.0	0.883866	63345	64182	1031-1031	1.000000	73.0
petA-psbJ_p4	petA-psbJ	4	CCATTACGTGTCCAAGGACT	GCTGGTATTCCTGTGATCGG	20	20	50.0	55.0	57.2	58.1	19.2	0.0	0.891455	63345	64182	1030-1030	1.000000	72.9
petA-psbJ_p5	petA-psbJ	5	AGGATCGGTTGTTTTGGCAC	GCTGGTATTCCTGTGATCGG	20	20	50.0	55.0	59.0	58.1	0.0	0.0	1.170098	63345	64182	998-998	1.000000	70.7
psbE-petL_p1	psbE-petL	1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	57.9	56.3	0.0	12.5	1.783771	64968	66364	1548-1549	1.000000	67.1
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	64968	66364	1466-1467	1.000000	66.5
psbE-petL_p3	psbE-petL	3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.865987	64968	66364	1549-1550	1.000000	66.4
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	20	20	55.0	45.0	58.2	56.3	0.9	12.5	1.909925	64968	66364	1461-1462	1.000000	66.1
psbE-petL_p5	psbE-petL	5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	20	21	45.0	42.9	57.9	57.0	0.0	12.5	2.027428	64968	66364	1548-1549	1.000000	65.1
rpl2_p1	rpl2	1	ACGTGTCACAGTTAATTAACTCCT	AAGGGGTCTTCAAAAGCGTT	24	20	37.5	45.0	58.1	57.9	24.8	0.0	4.216751	85172	86690	1686-1686	1.000000	46.3
rpl2_p2	rpl2	2	ACGTGTCACAGTTAATTAACTCC	AAGGGGTCTTCAAAAGCGTT	23	20	39.1	45.0	56.8	57.9	24.8	0.0	4.235945	85172	86690	1686-1686	1.000000	46.2
rpl2_p3	rpl2	3	AATGCTTTGCTACAAAAGGA	AAGGGGTCTTCAAAAGCGTT	20	20	35.0	45.0	53.7	57.9	0.0	0.0	4.411456	85172	86690	1720-1720	1.000000	44.8
rpl2_p4	rpl2	4	TGCTTTGCTACAAAAGGATT	AAGGGGTCTTCAAAAGCGTT	20	20	35.0	45.0	53.7	57.9	0.0	0.0	4.411456	85172	86690	1718-1718	1.000000	44.8
rpl2_p5	rpl2	5	ACGTGTCACAGTTAATTAACTCCT	CGCTTCAACCGGGTTATTCT	24	20	37.5	50.0	58.1	58.3	24.8	13.0	4.415223	85172	86690	1648-1648	1.000000	44.7
trnN-AAC-ycf1_p1	trnN-AAC-ycf1	1	CCCCAAGTAGGATTCGAACC	ATAGAGTCCGACCACAACGA	20	20	55.0	50.0	57.7	58.2	0.0	0.0	0.496921	109837	111006	1076-1324	1.000000	80.1
trnN-AAC-ycf1_p2	trnN-AAC-ycf1	2	CCCCAAGTAGGATTCGAACC	ACCACAACGACCGAATTGAT	20	20	55.0	45.0	57.7	57.8	0.0	0.0	0.506112	109837	111006	1066-1314	1.000000	80.0
trnN-AAC-ycf1_p3	trnN-AAC-ycf1	3	CCCCAAGTAGGATTCGAACC	GGGACCTATGGATAATGCGG	20	20	55.0	55.0	57.7	57.6	0.0	0.0	0.730452	109837	111006	1109-1357	1.000000	78.2
trnN-AAC-ycf1_p4	trnN-AAC-ycf1	4	CCCCAAGTAGGATTCGAACC	GACCACAACGACCGAATTGA	20	20	55.0	50.0	57.7	58.5	0.0	0.0	0.822841	109837	111006	1067-1315	1.000000	77.5
trnN-AAC-ycf1_p5	trnN-AAC-ycf1	5	CCCCAAGTAGGATTCGAACC	AATAGAGTCCGACCACAACG	20	20	55.0	50.0	57.7	57.4	0.0	0.0	0.976531	109837	111006	1077-1325	1.000000	76.3
ycf1_p1	ycf1	1	TGTGTAGTACATATCACCAAAA	CGGCTGGAATTTGTCAAACC	22	20	31.8	50.0	52.9	57.9	6.2	0.0	7.212514	115634	115783	314-314	1.000000	40.8
ycf1_p2	ycf1	2	TTGTGTAGTACATATCACCAAA	CGGCTGGAATTTGTCAAACC	22	20	31.8	50.0	52.9	57.9	6.2	0.0	7.212514	115634	115783	315-315	1.000000	40.8
ycf1_p3	ycf1	3	TTGTGTAGTACATATCACCAAAA	CGGCTGGAATTTGTCAAACC	23	20	30.4	50.0	53.7	57.9	6.2	0.0	7.362988	115634	115783	315-315	1.000000	40.8
ycf1_p4	ycf1	4	ATTGTGTAGTACATATCACCAA	CGGCTGGAATTTGTCAAACC	22	20	31.8	50.0	52.6	57.9	6.2	0.0	7.438889	115634	115783	316-316	1.000000	40.8
ycf1_p5	ycf1	5	TGCAGAAATACTATCTATTCTTCCTCA	CGGCTGGAATTTGTCAAACC	27	20	33.3	50.0	57.9	57.9	0.0	0.0	7.212306	115634	115783	382-382	1.000000	40.6
ndhD_p1	ndhD	1	AGGCTCTCACAATACAAAGACC	TGAGTACGCGTTCTTTGGAC	22	20	45.5	50.0	58.1	58.2	0.0	20.8	2.281275	124739	126259	1617-1617	1.000000	61.8
ndhD_p2	ndhD	2	GGCTCTCACAATACAAAGACCA	TGAGTACGCGTTCTTTGGAC	22	20	45.5	50.0	58.3	58.2	0.0	20.8	2.550365	124739	126259	1616-1616	1.000000	59.6
ndhD_p3	ndhD	3	AGGCTCTCACAATACAAAGACC	TTGAGTACGCGTTCTTTGGA	22	20	45.5	45.0	58.1	57.5	0.0	20.8	2.568398	124739	126259	1618-1618	1.000000	59.5
ndhD_p4	ndhD	4	GGCTCTCACAATACAAAGACC	TGAGTACGCGTTCTTTGGAC	21	20	47.6	50.0	56.6	58.2	0.0	20.8	2.610195	124739	126259	1616-1616	1.000000	59.2
ndhD_p5	ndhD	5	GGCTCTCACAATACAAAGACCA	TTGAGTACGCGTTCTTTGGA	22	20	45.5	45.0	58.3	57.5	0.0	20.8	2.837488	124739	126259	1617-1617	1.000000	57.3
ycf1-trnN-AAC_p1	ycf1-trnN-AAC	1	ATAGAGTCCGACCACAACGA	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	58.2	57.7	0.0	0.0	0.496921	138541	139710	1076-1324	1.000000	80.1
ycf1-trnN-AAC_p2	ycf1-trnN-AAC	2	ACCACAACGACCGAATTGAT	CCCCAAGTAGGATTCGAACC	20	20	45.0	55.0	57.8	57.7	0.0	0.0	0.506112	138541	139710	1066-1314	1.000000	80.0
ycf1-trnN-AAC_p3	ycf1-trnN-AAC	3	GGGACCTATGGATAATGCGG	CCCCAAGTAGGATTCGAACC	20	20	55.0	55.0	57.6	57.7	0.0	0.0	0.730452	138541	139710	1109-1357	1.000000	78.2
ycf1-trnN-AAC_p4	ycf1-trnN-AAC	4	GACCACAACGACCGAATTGA	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	58.5	57.7	0.0	0.0	0.822841	138541	139710	1067-1315	1.000000	77.5
ycf1-trnN-AAC_p5	ycf1-trnN-AAC	5	AATAGAGTCCGACCACAACG	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	57.4	57.7	0.0	0.0	0.976531	138541	139710	1077-1325	1.000000	76.3
ycf2_p1	ycf2	1	GACGGATAGAGGCGGATTTC	CTCGTCTCAGGTAGTCCCTC	20	20	55.0	60.0	57.9	58.3	0.0	0.0	0.396623	160486	160605	327-345	1.000000	78.5
ycf2_p2	ycf2	2	GACGGATAGAGGCGGATTTC	ATCTCGTCTCAGGTAGTCCC	20	20	55.0	55.0	57.9	57.6	0.0	0.0	0.419120	160486	160605	329-347	1.000000	78.3
ycf2_p3	ycf2	3	GACGGATAGAGGCGGATTTC	ATCCAACCCATCCTCGAACT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.482689	160486	160605	299-317	1.000000	78.0
ycf2_p4	ycf2	4	GACGGATAGAGGCGGATTTC	AATCCAACCCATCCTCGAAC	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.487472	160486	160605	300-318	1.000000	77.9
ycf2_p5	ycf2	5	ACGGATAGAGGCGGATTTCT	CTCGTCTCAGGTAGTCCCTC	20	20	50.0	60.0	58.3	58.3	0.0	0.0	0.623336	160486	160605	326-344	1.000000	76.7