primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5572	6736	1221-1238	1.000000	83.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5572	6736	1232-1249	1.000000	83.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5572	6736	1225-1242	1.000000	79.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	5572	6736	1233-1250	1.000000	78.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5572	6736	1213-1230	1.000000	78.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	TGATCAGAGAGAGCGGAGAG	AAAGTTTTTGGGGACGGGAA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.196330	8060	9122	1264-1355	1.000000	80.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AAGTGATCAGAGAGAGCGGA	AAAGTTTTTGGGGACGGGAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.298563	8060	9122	1267-1358	1.000000	79.9
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AATCTGCCGCTTTAGTCCAC	AAAGTTTTTGGGGACGGGAA	20	20	50.0	45.0	58.3	57.8	0.0	0.0	0.423197	8060	9122	1197-1288	1.000000	79.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GATCAGAGAGAGCGGAGAGA	AAAGTTTTTGGGGACGGGAA	20	20	55.0	45.0	57.7	57.8	0.0	0.0	0.416810	8060	9122	1263-1354	1.000000	79.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATCAGAGAGAGCGGAGAGAG	AAAGTTTTTGGGGACGGGAA	20	20	55.0	45.0	57.7	57.8	0.0	0.0	0.423108	8060	9122	1262-1353	1.000000	78.9
atpA_p1	atpA	1	TGATCTTGTACTAAGAAATGATCT	TTTTCCTTTGTTTCCGAAAAA	24	21	29.2	28.6	53.3	52.9	10.5	0.0	14.804250	10207	11730	1614-1614	0.500000	20.0
atpA_p2	atpA	2	TGATCTTGTACTAAGAAATGATCT	TTTTTCCTTTGTTTCCGAAAA	24	21	29.2	28.6	53.3	52.9	10.5	0.0	14.804250	10207	11730	1615-1615	0.500000	20.0
atpA_p3	atpA	3	TGATCTTGTACTAAGAAATGATCT	TTTTTCCTTTGTTTCCGAAAAA	24	22	29.2	27.3	53.3	53.8	10.5	0.0	14.929921	10207	11730	1615-1615	0.500000	20.0
atpA_p4	atpA	4	ATGATCTTGTACTAAGAAATGATCT	TTTTCCTTTGTTTCCGAAAAA	25	21	28.0	28.6	53.8	52.9	10.5	0.0	15.231285	10207	11730	1615-1615	0.500000	20.0
atpA_p5	atpA	5	ATGATCTTGTACTAAGAAATGATCT	TTTTTCCTTTGTTTCCGAAAA	25	21	28.0	28.6	53.8	52.9	10.5	0.0	15.231285	10207	11730	1616-1616	0.500000	20.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATCCGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	21	20	52.4	50.0	57.7	58.2	14.0	0.0	1.419371	44170	44717	660-660	1.000000	70.7
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATCCGGGATCTAGGC	TTCCAATGCTACGCCTTCAA	21	20	52.4	45.0	57.7	57.8	14.0	0.0	1.460686	44170	44717	609-609	1.000000	70.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCATTTATCCGGGATCTAGGC	AGTTCCAATGCTACGCCTTC	21	20	52.4	50.0	57.7	58.3	14.0	0.0	1.528956	44170	44717	611-611	1.000000	70.0
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	44170	44717	733-733	1.000000	68.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	44170	44717	734-734	1.000000	68.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGTATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	21	20	42.9	50.0	56.9	57.8	0.0	0.0	2.252840	50225	51819	1623-1650	1.000000	67.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCGTATTCGTGAAGCAGAAAC	AGAAGGTCTACGGTTCGAGT	21	20	42.9	50.0	56.7	57.8	0.0	0.0	2.522275	50225	51819	1624-1651	1.000000	65.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCGTATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	22	20	40.9	50.0	58.4	57.8	0.0	0.0	2.552460	50225	51819	1624-1651	1.000000	65.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CGTATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	21	20	42.9	55.0	56.9	57.5	0.0	0.0	2.596612	50225	51819	1622-1649	1.000000	64.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CGTATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	21	20	42.9	55.0	56.9	58.7	0.0	0.0	2.738906	50225	51819	1611-1638	1.000000	63.6
accD_p1	accD	1	CTATCCCGCAGTTTCAGACC	AAAGAAGCCATTGCAATCGC	20	20	55.0	45.0	58.1	58.0	0.0	19.9	0.059104	57767	59437	2152-2520	1.000000	87.5
accD_p2	accD	2	CTATCCCGCAGTTTCAGACC	CCGGGCCTTTTTAACCAGAT	20	20	55.0	50.0	58.1	58.2	0.0	0.8	0.211347	57767	59437	2389-2757	1.000000	86.2
accD_p3	accD	3	GGATCGTTTTCTATCCCGCA	AAAGAAGCCATTGCAATCGC	20	20	50.0	45.0	57.8	58.0	0.0	19.9	0.234729	57767	59437	2162-2530	1.000000	86.1
accD_p4	accD	4	ACTCGGCTCAATCCTTTTCC	AAAGAAGCCATTGCAATCGC	20	20	50.0	45.0	57.9	58.0	0.0	19.9	0.115772	57767	59437	2736-2736	0.500000	59.1
accD_p5	accD	5	CTATCCCGCAGTTTCAGACC	TATTCGCCGGGCCTTTTTAA	20	20	55.0	45.0	58.1	57.9	0.0	14.5	0.184448	57767	59437	2763-2763	0.500000	58.5
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.9	57.8	8.4	0.0	0.239365	60769	61407	647-729	1.000000	84.3
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	20	20	50.0	55.0	58.1	57.8	8.4	0.0	0.275442	60769	61407	649-731	1.000000	84.0
ycf4-cemA_p3	ycf4-cemA	3	GGAACCATTCCCTTGACTCG	ATGAGAGAGAGACCCACCAG	20	20	55.0	55.0	58.3	57.8	0.0	0.0	0.418573	60769	61407	729-811	1.000000	82.6
ycf4-cemA_p4	ycf4-cemA	4	TTTCTTGCGCGTACCAATTG	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.7	57.8	8.4	0.0	0.481579	60769	61407	652-734	1.000000	82.3
ycf4-cemA_p5	ycf4-cemA	5	CCTATTTCTTGCGCGTACCA	ATGAGAGAGAGACCCACCAG	20	20	50.0	55.0	58.4	57.8	8.4	0.0	0.510294	60769	61407	656-738	1.000000	82.1
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.169530	66561	66708	204-209	1.000000	82.5
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	66561	66708	202-207	1.000000	82.5
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.170864	66561	66708	205-210	1.000000	82.5
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.161494	66561	66708	215-220	1.000000	82.4
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.162838	66561	66708	216-221	1.000000	82.4
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	84153	84195	179-179	1.000000	80.1
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	84153	84195	178-178	1.000000	80.1
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	84153	84195	180-180	1.000000	80.1
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	84153	84195	193-193	1.000000	80.0
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	TATCCTGCGCTTGGAAGAAG	20	20	55.0	50.0	58.1	57.7	0.2	22.4	0.444845	84153	84195	191-191	1.000000	77.7
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	107593	107813	335-338	1.000000	82.7
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	107593	107813	333-336	1.000000	82.7
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	107593	107813	331-334	1.000000	82.7
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	GCTGAGGCATCCTAACAGAC	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	107593	107813	333-336	1.000000	82.6
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	CAGCTGAGGCATCCTAACAG	TCCCCTACAGTATCGTCACC	20	20	55.0	55.0	58.0	57.9	8.4	0.0	0.112142	107593	107813	343-346	1.000000	82.4
ndhF-rpl32_p1	ndhF-rpl32	1	CTGCAATTGGTCGTGTGAAC	AGGACTAGACCGAGTGTACAG	20	21	50.0	52.4	58.0	58.0	7.7	5.6	1.047987	112371	113301	1772-1788	1.000000	76.2
ndhF-rpl32_p2	ndhF-rpl32	2	CTGCAATTGGTCGTGTGAAC	GGACTAGACCGAGTGTACAGA	20	21	50.0	52.4	58.0	58.0	7.7	0.0	1.053328	112371	113301	1771-1787	1.000000	76.2
ndhF-rpl32_p3	ndhF-rpl32	3	GCGCAGATTTAGCAATTGCA	AGGACTAGACCGAGTGTACAG	20	21	45.0	52.4	58.1	58.0	30.9	5.6	1.099429	112371	113301	1985-2001	1.000000	75.4
ndhF-rpl32_p4	ndhF-rpl32	4	TGCGCAGATTTAGCAATTGC	AGGACTAGACCGAGTGTACAG	20	21	45.0	52.4	58.1	58.0	23.1	5.6	1.099429	112371	113301	1986-2002	1.000000	75.4
ndhF-rpl32_p5	ndhF-rpl32	5	GCGCAGATTTAGCAATTGCA	GGACTAGACCGAGTGTACAGA	20	21	45.0	52.4	58.1	58.0	30.9	0.0	1.104770	112371	113301	1984-2000	1.000000	75.3
ccsA-ndhD_p1	ccsA-ndhD	1	AGTTATGGTTCATTCACATGA	TGACAAGGTACAAGCTATCCT	21	21	33.3	42.9	52.8	56.1	0.0	0.0	9.103553	115204	115447	307-308	1.000000	49.4
ccsA-ndhD_p2	ccsA-ndhD	2	AGTTATGGTTCATTCACATGA	TGACAAGGTACAAGCTATCC	21	20	33.3	45.0	52.8	54.5	0.0	0.0	9.703261	115204	115447	307-308	1.000000	49.4
ccsA-ndhD_p3	ccsA-ndhD	3	AGTTATGGTTCATTCACATGA	GACAAGGTACAAGCTATCCT	21	20	33.3	45.0	52.8	54.2	0.0	0.0	10.014134	115204	115447	306-307	1.000000	49.4
ccsA-ndhD_p4	ccsA-ndhD	4	AGTTATGGTTCATTCACATGA	TTGACAAGGTACAAGCTATCCT	21	22	33.3	40.9	52.8	56.8	0.0	0.0	9.358674	115204	115447	308-309	1.000000	49.3
ccsA-ndhD_p5	ccsA-ndhD	5	AGTTATGGTTCATTCACATGA	TTGACAAGGTACAAGCTATCC	21	21	33.3	42.9	52.8	55.3	0.0	0.0	9.860219	115204	115447	308-309	1.000000	49.3
ndhD_p1	ndhD	1	TGCTTTCTATCTCATTAATGAAAA	ACCGAGAAAAACCCGTACTC	24	20	25.0	50.0	52.8	57.6	18.5	0.0	9.680748	115448	116950	1605-1605	1.000000	41.7
ndhD_p2	ndhD	2	TTGCTTTCTATCTCATTAATGAAA	ACCGAGAAAAACCCGTACTC	24	20	25.0	50.0	52.8	57.6	18.5	0.0	9.680748	115448	116950	1606-1606	1.000000	41.7
ndhD_p3	ndhD	3	TTTGCTTTCTATCTCATTAATGAA	ACCGAGAAAAACCCGTACTC	24	20	25.0	50.0	52.8	57.6	18.5	0.0	9.680748	115448	116950	1607-1607	1.000000	41.7
ndhD_p4	ndhD	4	TGCTTTCTATCTCATTAATGAAAA	CCGAGAAAAACCCGTACTCG	24	20	25.0	55.0	52.8	58.7	18.5	0.0	9.891677	115448	116950	1604-1604	1.000000	41.7
ndhD_p5	ndhD	5	TTGCTTTCTATCTCATTAATGAAA	CCGAGAAAAACCCGTACTCG	24	20	25.0	55.0	52.8	58.7	18.5	0.0	9.891677	115448	116950	1605-1605	1.000000	41.7
ycf1_p1	ycf1	1	CTCTACGACGTCTGGGAGAT	ACCTTGACCAATACGTATGCC	20	21	55.0	47.6	58.0	58.1	14.7	9.2	1.129644	124570	124689	814-814	0.500000	51.0
ycf1_p2	ycf1	2	CTCTACGACGTCTGGGAGAT	CCTTGACCAATACGTATGCCA	20	21	55.0	47.6	58.0	58.1	14.7	9.2	1.130932	124570	124689	813-813	0.500000	51.0
ycf1_p3	ycf1	3	TTCTCTACGACGTCTGGGAG	ACCTTGACCAATACGTATGCC	20	21	55.0	47.6	58.3	58.1	14.7	9.2	1.350555	124570	124689	816-816	0.500000	49.2
ycf1_p4	ycf1	4	TTCTCTACGACGTCTGGGAG	CCTTGACCAATACGTATGCCA	20	21	55.0	47.6	58.3	58.1	14.7	9.2	1.351843	124570	124689	815-815	0.500000	49.2
ycf1_p5	ycf1	5	ACGACGTCTGGGAGATAGAA	ACCTTGACCAATACGTATGCC	20	21	50.0	47.6	57.6	58.1	14.7	9.2	1.500934	124570	124689	810-810	0.500000	48.0
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	130440	130660	335-338	1.000000	82.7
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	130440	130660	333-336	1.000000	82.7
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.087359	130440	130660	331-334	1.000000	82.7
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	AGTATCGTCACCGCAGTAGA	GCTGAGGCATCCTAACAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.087359	130440	130660	333-336	1.000000	82.6
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	TCCCCTACAGTATCGTCACC	CAGCTGAGGCATCCTAACAG	20	20	55.0	55.0	57.9	58.0	0.0	8.4	0.112142	130440	130660	343-346	1.000000	82.4
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	154058	154100	179-179	1.000000	80.1
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	154058	154100	178-178	1.000000	80.1
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	154058	154100	180-180	1.000000	80.1
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	154058	154100	193-193	1.000000	80.0
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	TATCCTGCGCTTGGAAGAAG	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	57.7	58.1	22.4	0.2	0.444845	154058	154100	191-191	1.000000	77.7